BaheA.00189.a
dihydrodipicolinate reductase
| CENTER ID: | BaheA.00189.a |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00189.a.A1.GE25694 | Full length( BaheA.00189.a ) | 1 | 267 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00189.a.A1.PS00326 | Full length( BaheA.00189.a ) | 1 | 267 |
Structures
3IJP
DEPOSITED: 8/4/2009
DETERMINATION: XRay
CLONE: BaheA.00189.a.A1.GE25694
PROTEIN: BaheA.00189.a.A1.PS00326
Publications by SSGCID
The crystal structure of dihydrodipicolinate reductase from the human-pathogenic bacterium Bartonella henselae strain Houston-1 at 2.3 A resolution
Abendroth J, Hudson AO, Myler P, Staker B, Reers A, Cala A, Nadeau M, Weatherhead A, Dobson R
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2016
volume 72, issue 12, pages 886-891
PMID: 27917836; PMCID: PMC5137465
PDB:
3IJP
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131385 |
| PATRIC ID: | fig|283166.5.peg.1313 |
| RefSeq: | YP_033998.1 |
| UniProt: | Q6G2G3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLTVVGANG RMGRELITAI QRRKDVELCA VLVRKGSSFV DKDASILIGS DFLGVRITDD PESAFSNTEG ILDFSQPQAS VLYANYAAQK SLIHIIGTTG FSKTEEAQIA DFAKYTTIVK SGNMSLGVNL LANLVKRAAK ALDDDFDIEI YEMHHANKVD SPSGTALLLG QAAAEGRNIM LKNVSVNGRS GHTGKREKGT IGFACSRGGT VIGDHSITFA GENERIVLSH IAQERSIFAN GALKAALWAK NHENGLYSML DVLGLNE
NT Sequence
ATGCGCCTTA CAGTTGTTGG TGCGAATGGA AGAATGGGAC GCGAACTTAT TACAGCTATT CAGCGTAGGA AAGACGTAGA ACTTTGTGCT GTGCTTGTAC GAAAAGGATC ATCTTTTGTA GATAAAGATG CCAGTATATT GATTGGTTCA GATTTTCTCG GTGTCCGCAT CACTGATGAT CCAGAAAGCG CCTTTTCTAA TACTGAAGGT ATTTTAGATT TTTCTCAACC ACAAGCAAGT GTTCTCTATG CCAACTATGC TGCTCAAAAA AGTCTTATTC ATATTATTGG CACAACAGGG TTTAGTAAAA CAGAAGAAGC GCAAATTGCA GACTTTGCAA AATATACAAC TATTGTCAAA TCCGGAAATA TGAGTCTTGG AGTTAATCTC TTGGCAAATC TTGTAAAGAG AGCCGCAAAA GCATTAGATG ATGATTTCGA TATTGAAATT TATGAAATGC ATCACGCGAA CAAAGTTGAT TCTCCTTCTG GAACAGCTCT CCTTCTTGGG CAAGCAGCCG CCGAAGGCCG CAATATTATG CTAAAAAATG TGAGTGTCAA CGGACGTAGT GGCCATACAG GGAAACGTGA AAAAGGCACA ATTGGCTTTG CCTGTTCACG AGGTGGAACA GTTATTGGCG ATCATAGCAT CACTTTTGCA GGCGAAAATG AACGTATCGT TCTTTCACAC ATAGCACAAG AACGTTCTAT TTTTGCCAAT GGCGCATTAA AAGCAGCTTT ATGGGCAAAG AACCACGAAA ATGGTCTTTA TTCAATGCTT GATGTTTTGG GACTGAACGA A
Details for BaheA.00189.a.A1.GE25694
| HARVESTED ON: | 10/17/2008 |
| SEQUENCED ON: | 2/8/2016 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLTVVGAN GRMGRELITA IQRRKDVELC AVLVRKGSSF VDKDASILIG SDFLGVRITD DPESAFSNTE GILDFSQPQA SVLYANYAAQ KSLIHIIGTT GFSKTEEAQI ADFAKYTTIV KSGNMSLGVN LLANLVKRAA KALDDDFDIE IYEMHHANKV DSPSGTALLL GQAAAEGRNI MLKNVSVNGR SGHTGKREKG TIGFACSRGG TVIGDHSITF AGENERIVLS HIAQERSIFA NGALKAALWA KNHENGLYSM LDVLGL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc ttacagttgt tggtgcgaat ggaagaatgg gacgcgaact tattacagct attcagcgta ggaaagacgt agaactttgt gctgtgcttg tacgaaaagg atcatctttt gtagataaag atgccagtat attgattggt tcagattttc tcggtgtccg catcactgat gatccagaaa gcgccttttc taatactgaa ggtattttag atttttctca accacaagca agtgttctct atgccaacta tgctgctcaa aaaagtctta ttcatattat tggcacaaca gggtttagta aaacagaaga agcgcaaatt gcagactttg caaaatatac aactattgtc aaatccggaa atatgagtct tggagttaat ctcttggcaa atcttgtaaa gagagccgca aaagcattag atgatgattt cgatattgaa atttatgaaa tgcatcacgc gaacaaagtt gattctcctt ctggaacagc tctccttctt gggcaagcag ccgccgaagg ccgcaatatt atgctaaaaa atgtgagtgt caacggacgt agtggccata cagggaaacg tgaaaaaggc acaattggct ttgcctgttc acgaggtgga acagttattg gcgatcatag catcactttt gcaggcgaaa atgaacgtat cgttctttca cacatagcac aagaacgttc tatttttgcc aatggcgcat taaaagcagc tttatgggca aagaaccacg aaaatggtct ttattcaatg cttgatgttt tgggactga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLTVVGAN GRMGRELITA IQRRKDVELC AVLVRKGSSF VDKDASILIG SDFLGVRITD DPESAFSNTE GILDFSQPQA SVLYANYAAQ KSLIHIIGTT GFSKTEEAQI ADFAKYTTIV KSGNMSLGVN LLANLVKRAA KALDDDFDIE IYEMHHANKV DSPSGTALLL GQAAAEGRNI MLKNVSVNGR SGHTGKREKG TIGFACSRGG TVIGDHSITF AGENERIVLS HIAQERSIFA NGALKAALWA KNHENGLYSM LDVLGLNE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgccttaca gttgttggtg cgaatggaag aatgggacgc gaacttatta cagctattca gcgtaggaaa gacgtagaac tttgtgctgt gcttgtacga aaaggatcat cttttgtaga taaagatgcc agtatattga ttggttcaga ttttctcggt gtccgcatca ctgatgatcc agaaagcgcc ttttctaata ctgaaggtat tttagatttt tctcaaccac aagcaagtgt tctctatgcc aactatgctg ctcaaaaaag tcttattcat attattggca caacagggtt tagtaaaaca gaagaagcgc aaattgcaga ctttgcaaaa tatacaacta ttgtcaaatc cggaaatatg agtcttggag ttaatctctt ggcaaatctt gtaaagagag ccgcaaaagc attagatgat gatttcgata ttgaaattta tgaaatgcat cacgcgaaca aagttgattc tccttctgga acagctctcc ttcttgggca agcagccgcc gaaggccgca atattatgct aaaaaatgtg agtgtcaacg gacgtagtgg ccatacaggg aaacgtgaaa aaggcacaat tggctttgcc tgttcacgag gtggaacagt tattggcgat catagcatca cttttgcagg cgaaaatgaa cgtatcgttc tttcacacat agcacaagaa cgttctattt ttgccaatgg cgcattaaaa gcagctttat gggcaaagaa ccacgaaaat ggtctttatt caatgcttga tgttttggga ctgaacgaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BaheA.00189.a.A1.PS00326
| PURIFICATION DATe: | 6/22/2009 |
| CONCENTRATION: | 6.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLTVVGAN GRMGRELITA IQRRKDVELC AVLVRKGSSF VDKDASILIG SDFLGVRITD DPESAFSNTE GILDFSQPQA SVLYANYAAQ KSLIHIIGTT GFSKTEEAQI ADFAKYTTIV KSGNMSLGVN LLANLVKRAA KALDDDFDIE IYEMHHANKV DSPSGTALLL GQAAAEGRNI MLKNVSVNGR SGHTGKREKG TIGFACSRGG TVIGDHSITF AGENERIVLS HIAQERSIFA NGALKAALWA KNHENGLYSM LDVLGL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc ttacagttgt tggtgcgaat ggaagaatgg gacgcgaact tattacagct attcagcgta ggaaagacgt agaactttgt gctgtgcttg tacgaaaagg atcatctttt gtagataaag atgccagtat attgattggt tcagattttc tcggtgtccg catcactgat gatccagaaa gcgccttttc taatactgaa ggtattttag atttttctca accacaagca agtgttctct atgccaacta tgctgctcaa aaaagtctta ttcatattat tggcacaaca gggtttagta aaacagaaga agcgcaaatt gcagactttg caaaatatac aactattgtc aaatccggaa atatgagtct tggagttaat ctcttggcaa atcttgtaaa gagagccgca aaagcattag atgatgattt cgatattgaa atttatgaaa tgcatcacgc gaacaaagtt gattctcctt ctggaacagc tctccttctt gggcaagcag ccgccgaagg ccgcaatatt atgctaaaaa atgtgagtgt caacggacgt agtggccata cagggaaacg tgaaaaaggc acaattggct ttgcctgttc acgaggtgga acagttattg gcgatcatag catcactttt gcaggcgaaa atgaacgtat cgttctttca cacatagcac aagaacgttc tatttttgcc aatggcgcat taaaagcagc tttatgggca aagaaccacg aaaatggtct ttattcaatg cttgatgttt tgggactga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLTVVGAN GRMGRELITA IQRRKDVELC AVLVRKGSSF VDKDASILIG SDFLGVRITD DPESAFSNTE GILDFSQPQA SVLYANYAAQ KSLIHIIGTT GFSKTEEAQI ADFAKYTTIV KSGNMSLGVN LLANLVKRAA KALDDDFDIE IYEMHHANKV DSPSGTALLL GQAAAEGRNI MLKNVSVNGR SGHTGKREKG TIGFACSRGG TVIGDHSITF AGENERIVLS HIAQERSIFA NGALKAALWA KNHENGLYSM LDVLGLNE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCTTACA GTTGTTGGTG CGAATGGAAG AATGGGACGC GAACTTATTA CAGCTATTCA GCGTAGGAAA GACGTAGAAC TTTGTGCTGT GCTTGTACGA AAAGGATCAT CTTTTGTAGA TAAAGATGCC AGTATATTGA TTGGTTCAGA TTTTCTCGGT GTCCGCATCA CTGATGATCC AGAAAGCGCC TTTTCTAATA CTGAAGGTAT TTTAGATTTT TCTCAACCAC AAGCAAGTGT TCTCTATGCC AACTATGCTG CTCAAAAAAG TCTTATTCAT ATTATTGGCA CAACAGGGTT TAGTAAAACA GAAGAAGCGC AAATTGCAGA CTTTGCAAAA TATACAACTA TTGTCAAATC CGGAAATATG AGTCTTGGAG TTAATCTCTT GGCAAATCTT GTAAAGAGAG CCGCAAAAGC ATTAGATGAT GATTTCGATA TTGAAATTTA TGAAATGCAT CACGCGAACA AAGTTGATTC TCCTTCTGGA ACAGCTCTCC TTCTTGGGCA AGCAGCCGCC GAAGGCCGCA ATATTATGCT AAAAAATGTG AGTGTCAACG GACGTAGTGG CCATACAGGG AAACGTGAAA AAGGCACAAT TGGCTTTGCC TGTTCACGAG GTGGAACAGT TATTGGCGAT CATAGCATCA CTTTTGCAGG CGAAAATGAA CGTATCGTTC TTTCACACAT AGCACAAGAA CGTTCTATTT TTGCCAATGG CGCATTAAAA GCAGCTTTAT GGGCAAAGAA CCACGAAAAT GGTCTTTATT CAATGCTTGA TGTTTTGGGA CTGAACGAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A