SSGCID: Target Page

BaheA.00171.a

3-oxoacyl-(acyl carrier protein) synthase III

CENTER ID:	BaheA.00171.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00171.a.A1.GE25192	Full length( BaheA.00171.a )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00171.a.A1.PS00030	Full length( BaheA.00171.a )		1	324

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129509
PATRIC ID:	fig\|283166.5.peg.816
RefSeq:	YP_033575.1
UniProt:	Q6G3K4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIRSVVRGIG GALPKKSLSN DDIAKFVETS DSWIVQRTGI RQRYIANEKE TTVSLGVEAA QAALINAGLT IKDIDCIILA TSTPNHTFPA SAVEIQHALG MSHGFAFDIQ AVCSGFIFAL TTGDAYLRCG TAKRILVIGS DTFSRILDWQ DRTTCVLFGD GAGAAVLEAQ EIERGVASDH GILSTKLRSN GAYMDKLYVD GGPSTTQKTG YLRMEGREVF KYAVGMITDV VDECFAAAGM DSSQLDWFVP HQANKRIIEA SAKKLGISID KVVITVDRHG NTSAASVPLA LTMAICDGKI KKGDLVMLEA MGGGFTWGAI LIRW

NT Sequence

ATGATTCGAT CTGTTGTGCG TGGTATAGGA GGTGCCTTAC CCAAAAAAAG CTTATCGAAT GATGATATTG CGAAGTTTGT TGAAACATCA GATTCTTGGA TTGTTCAGCG TACGGGAATA CGCCAACGCT ACATTGCCAA TGAAAAGGAA ACAACAGTTT CTTTGGGAGT AGAAGCTGCA CAAGCTGCAC TTATAAATGC TGGTTTAACA ATTAAAGATA TTGATTGTAT TATTTTGGCG ACATCAACAC CAAATCACAC TTTTCCCGCT TCTGCTGTCG AAATTCAACA TGCTTTGGGA ATGAGCCATG GATTTGCGTT TGATATTCAG GCTGTTTGCT CGGGTTTTAT TTTTGCTTTA ACAACAGGAG ATGCTTATTT ACGCTGTGGA ACAGCCAAAA GGATTTTGGT TATCGGATCT GACACATTTT CTCGAATTTT AGATTGGCAA GATCGTACGA CATGTGTTTT GTTCGGTGAT GGTGCGGGTG CTGCTGTTTT AGAAGCACAA GAAATTGAGA GAGGCGTTGC TTCTGATCAT GGCATTTTAT CTACAAAATT GCGCTCTAAT GGTGCGTATA TGGATAAACT TTATGTTGAT GGTGGTCCTT CAACAACGCA AAAAACTGGC TATTTACGTA TGGAAGGACG AGAAGTTTTT AAATATGCAG TTGGTATGAT AACCGATGTA GTTGATGAGT GTTTTGCAGC TGCAGGTATG GATTCTTCAC AATTAGATTG GTTTGTACCT CATCAGGCCA ATAAACGTAT TATTGAAGCG TCAGCTAAAA AACTAGGAAT TTCTATAGAT AAAGTGGTAA TCACCGTTGA TCGACACGGT AATACGTCTG CAGCATCTGT GCCATTGGCT TTAACAATGG CTATTTGCGA TGGAAAAATT AAAAAAGGAG ATCTTGTCAT GTTAGAAGCT ATGGGAGGAG GGTTTACATG GGGTGCAATT CTTATTCGTT GG

Details for BaheA.00171.a.A1.GE25192

HARVESTED ON:	5/6/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	37kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	67

Validated AA Sequence

MGTLEAQTQG PGSMIRSVVR GIGGALPKKS LSNDDIAKFV ETSDSWIVQR TGIRQRYIAN EKETTVSLGV EAAQAALINA GLTIKDIDCI ILATSTPNHT FPASAVEIQH ALGMSHGFAF DIQAVCSGFI FALTTGDAYL RCGTAKRILV IGSDTFSRIL DWQDRTTCVL FGDGAGAAVL EAQEIERGVA SDHGILSTKL RSNGAYMDKL YVDGGSFNNA KNWLFTYGXX RSF

Validated NT Sequence

accntggctc ntcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga ttcgatctgt tgtgcgtggt ataggaggtg ccttacccaa aaaaagctta tcgaatgatg atattgcgaa gtttgttgaa acatcagatt cttggattgt tcagcgtacg ggaatacgcc aacgctacat tgccaatgaa aaggaaacaa cagtttcttt gggagtagaa gctgcacaag ctgcacttat aaatgctggt ttaacaatta aagatattga ttgtattatt ttggcgacat caacaccaaa tcacactttt cccgcttctg ctgtcgaaat tcaacatgct ttgggaatga gccatggatt tgcgtttgat attcaggctg tttgctcggg ttttattttt gctttaacaa caggagatgc ttatttacgc tgtggaacag ccaaaaggat tttggttatc ggatctgaca cattttctcg aattttagat tggcaagatc gtacgacatg tgttttgttc ggtgatggtg cgggtgctgc tgttttagaa gcacaagaaa ttgagagagg cgttgcttct gatcatggca ttttatctac aaaattgcgc tctaatggtg cgtatatgga taaactttat gttgatggtg gntccttcaa caacgcaaaa aactggctat ttacgtatgg aangannaga agtttttaaa tatgcagttg gntatgataa ccgatgtagt tgatgagtgt tttgcagctg cnngtatgga ttcttcacna ttanattggt ttgtacctca tcangccnat naacntatta ttgaancgtc nnctnaaaac tangaatttc tata

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIRSVVRGI GGALPKKSLS NDDIAKFVET SDSWIVQRTG IRQRYIANEK ETTVSLGVEA AQAALINAGL TIKDIDCIIL ATSTPNHTFP ASAVEIQHAL GMSHGFAFDI QAVCSGFIFA LTTGDAYLRC GTAKRILVIG SDTFSRILDW QDRTTCVLFG DGAGAAVLEA QEIERGVASD HGILSTKLRS NGAYMDKLYV DGGPSTTQKT GYLRMEGREV FKYAVGMITD VVDECFAAAG MDSSQLDWFV PHQANKRIIE ASAKKLGISI DKVVITVDRH GNTSAASVPL ALTMAICDGK IKKGDLVMLE AMGGGFTWGA ILIRW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcgatct gttgtgcgtg gtataggagg tgccttaccc aaaaaaagct tatcgaatga tgatattgcg aagtttgttg aaacatcaga ttcttggatt gttcagcgta cgggaatacg ccaacgctac attgccaatg aaaaggaaac aacagtttct ttgggagtag aagctgcaca agctgcactt ataaatgctg gtttaacaat taaagatatt gattgtatta ttttggcgac atcaacacca aatcacactt ttcccgcttc tgctgtcgaa attcaacatg ctttgggaat gagccatgga tttgcgtttg atattcaggc tgtttgctcg ggttttattt ttgctttaac aacaggagat gcttatttac gctgtggaac agccaaaagg attttggtta tcggatctga cacattttct cgaattttag attggcaaga tcgtacgaca tgtgttttgt tcggtgatgg tgcgggtgct gctgttttag aagcacaaga aattgagaga ggcgttgctt ctgatcatgg cattttatct acaaaattgc gctctaatgg tgcgtatatg gataaacttt atgttgatgg tggtccttca acaacgcaaa aaactggcta tttacgtatg gaaggacgag aagtttttaa atatgcagtt ggtatgataa ccgatgtagt tgatgagtgt tttgcagctg caggtatgga ttcttcacaa ttagattggt ttgtacctca tcaggccaat aaacgtatta ttgaagcgtc agctaaaaaa ctaggaattt ctatagataa agtggtaatc accgttgatc gacacggtaa tacgtctgca gcatctgtgc cattggcttt aacaatggct atttgcgatg gaaaaattaa aaaaggagat cttgtcatgt tagaagctat gggaggaggg tttacatggg gtgcaattct tattcgttgg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BaheA.00171.a.A1.PS00030

PURIFICATION DATe:	6/24/2008
CONCENTRATION:	8.74mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	100µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	67

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTCGATCT GTTGTGCGTG GTATAGGAGG TGCCTTACCC AAAAAAAGCT TATCGAATGA TGATATTGCG AAGTTTGTTG AAACATCAGA TTCTTGGATT GTTCAGCGTA CGGGAATACG CCAACGCTAC ATTGCCAATG AAAAGGAAAC AACAGTTTCT TTGGGAGTAG AAGCTGCACA AGCTGCACTT ATAAATGCTG GTTTAACAAT TAAAGATATT GATTGTATTA TTTTGGCGAC ATCAACACCA AATCACACTT TTCCCGCTTC TGCTGTCGAA ATTCAACATG CTTTGGGAAT GAGCCATGGA TTTGCGTTTG ATATTCAGGC TGTTTGCTCG GGTTTTATTT TTGCTTTAAC AACAGGAGAT GCTTATTTAC GCTGTGGAAC AGCCAAAAGG ATTTTGGTTA TCGGATCTGA CACATTTTCT CGAATTTTAG ATTGGCAAGA TCGTACGACA TGTGTTTTGT TCGGTGATGG TGCGGGTGCT GCTGTTTTAG AAGCACAAGA AATTGAGAGA GGCGTTGCTT CTGATCATGG CATTTTATCT ACAAAATTGC GCTCTAATGG TGCGTATATG GATAAACTTT ATGTTGATGG TGGTCCTTCA ACAACGCAAA AAACTGGCTA TTTACGTATG GAAGGACGAG AAGTTTTTAA ATATGCAGTT GGTATGATAA CCGATGTAGT TGATGAGTGT TTTGCAGCTG CAGGTATGGA TTCTTCACAA TTAGATTGGT TTGTACCTCA TCAGGCCAAT AAACGTATTA TTGAAGCGTC AGCTAAAAAA CTAGGAATTT CTATAGATAA AGTGGTAATC ACCGTTGATC GACACGGTAA TACGTCTGCA GCATCTGTGC CATTGGCTTT AACAATGGCT ATTTGCGATG GAAAAATTAA AAAAGGAGAT CTTGTCATGT TAGAAGCTAT GGGAGGAGGG TTTACATGGG GTGCAATTCT TATTCGTTGG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA