BaheA.00157.a

DNA topoisomerase IV subunit A

CENTER ID: BaheA.00157.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00157.a.A1.GE25186 Full length( BaheA.00157.a ) 1 749
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00157.a.A1.PS00084 Full length( BaheA.00157.a ) 1 749
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_140520
PATRIC ID: fig|283166.5.peg.1025
RefSeq: YP_033769.1
UniProt: A0A0H3LXK1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDKMNLPFD KEHIESIELR SALQERYLAY ALSTITHRAL PDVRDGLKPV HRRIVHAMRL LKLNPGHSYA KCARIVGDVM GKFHPHGDAS IYDALVRLAQ NFAVRYPLVD GQGNFGNIDG DNAAAMRYTE ARMTEVAALL LEGINENAID FRLTYNEEDE EPIVLPGAFP NLLANGSSGI AVGMATSIPP HNVSELCDAA LHLIAHPNAR DEELNHFVLG PDFPTGGILV EPKKSIEESY RVGRGFFRLR SRWHQETGNR GSYVLVVTEI PYQVQKSRLI EKTAELLLAR RLPMLEDIRD ESAEDIRIVL IPKNRTVDPE ILMESLFKLT DFEIRFPLNL NVLSFGKVPN VLSLRESLKQ WLEHRQEVLI RRSRYQLDEI ERRLEILHGY LITYLNLDEV IQIIREEDEP KKKLIDRFEL TENQAEAILN MRLRSLRKLE EFEIRKEFET LRAEKEKLQN LLDSRTKQWK IISEEIIKVR KVFGPHTLLG KRRTTFEEAP RHDFDDIYQA MIEKEPVTIV ISEKGWMRAL KGHLSDYNAL SFKEGDCLKL AFPAFTTDKI VVISTGGKFF AIAANSLPGG RGHGEPIRIL VDMDDEHDLL TAFVHHTQEK LLLVSSHGNG FIVPATEVIA NTRKGKQVMN VKFPDKVKLC VPVKGDHVAV VGENRKMLIF AIEQIPEMSR GKGVRLQRYK QGGVVDVKTF NLSEGLSWQD TAERCFNRQA SDLIEWMGVR SGVGRVVPKG FPKSGKFTN
NT Sequence
ATGTCTGATA AAATGAATTT ACCTTTTGAT AAAGAACATA TTGAGTCAAT AGAATTACGT TCTGCTTTAC AAGAGCGTTA TTTGGCTTAT GCGCTTTCTA CTATTACGCA CCGTGCGTTG CCTGATGTGC GTGATGGTTT GAAACCTGTG CATAGGCGTA TTGTTCACGC TATGCGTTTA CTCAAACTTA ATCCAGGACA TTCTTATGCG AAATGCGCAA GGATTGTTGG GGATGTCATG GGAAAATTTC ATCCACATGG TGATGCATCT ATTTATGATG CATTAGTGCG CTTGGCTCAG AATTTTGCTG TCCGTTATCC ATTGGTTGAT GGACAAGGCA ATTTTGGGAA TATTGATGGT GATAATGCTG CTGCTATGCG TTATACGGAA GCACGCATGA CTGAAGTAGC TGCATTGCTG CTTGAAGGAA TTAATGAAAA CGCTATTGAT TTTCGTTTAA CCTATAATGA GGAAGATGAA GAACCGATCG TTTTACCTGG AGCTTTTCCT AATCTTTTAG CAAATGGTTC ATCAGGTATT GCTGTTGGTA TGGCAACATC TATTCCACCC CATAATGTTT CTGAACTTTG TGATGCGGCG CTTCATTTGA TTGCGCATCC CAATGCCCGT GATGAGGAAT TAAATCACTT TGTGCTTGGA CCTGATTTTC CAACAGGTGG CATTTTAGTT GAACCTAAAA AGAGTATTGA GGAATCTTAT CGTGTTGGGC GTGGTTTTTT TCGGTTGCGT TCGCGTTGGC ATCAGGAAAC CGGTAACCGT GGTTCTTATG TGCTTGTTGT GACTGAAATT CCCTATCAAG TTCAAAAGTC ACGACTTATT GAAAAAACAG CAGAATTATT GCTTGCACGG CGGTTACCAA TGCTTGAAGA TATTCGTGAT GAGTCAGCAG AAGATATTCG CATTGTACTT ATTCCTAAAA ATCGTACCGT CGATCCTGAG ATTCTTATGG AGTCTCTTTT TAAATTGACT GATTTTGAAA TAAGATTTCC TCTCAATCTT AATGTTTTGT CTTTTGGAAA AGTACCGAAT GTTCTTTCTT TGCGGGAAAG TTTAAAACAA TGGCTTGAAC ATCGTCAAGA GGTGCTTATT CGCCGTTCTC GTTATCAGCT TGACGAAATT GAACGACGGT TGGAAATCCT CCATGGATAT CTTATCACAT ATTTGAATCT TGATGAGGTG ATCCAGATTA TTCGGGAAGA GGATGAACCA AAAAAAAAGC TTATCGATCG TTTTGAATTA ACAGAAAATC AAGCTGAAGC TATTCTTAAT ATGCGCTTGC GTTCTTTACG TAAACTTGAG GAATTTGAAA TTCGTAAAGA ATTTGAAACC TTGAGGGCTG AAAAAGAAAA ACTGCAAAAT TTGTTAGATT CTCGGACAAA GCAATGGAAA ATAATTTCAG AGGAAATCAT AAAAGTTCGT AAAGTTTTTG GTCCTCATAC TCTCTTAGGG AAAAGGCGTA CAACATTTGA AGAAGCACCA AGACATGATT TTGATGATAT TTATCAGGCA ATGATTGAAA AAGAACCAGT TACTATTGTT ATTTCTGAAA AGGGTTGGAT GCGTGCTTTG AAGGGGCATT TGAGTGATTA CAATGCACTT TCTTTCAAAG AAGGGGATTG TTTGAAATTA GCATTTCCAG CGTTTACTAC AGATAAAATT GTGGTGATAA GTACTGGCGG AAAGTTTTTT GCTATTGCTG CAAATAGTTT GCCGGGAGGA CGGGGGCATG GGGAACCCAT TCGTATTTTA GTTGATATGG ATGACGAGCA TGATCTCCTG ACAGCTTTTG TGCATCATAC ACAGGAAAAA CTTCTCTTAG TTTCATCTCA TGGAAATGGT TTTATTGTAC CTGCAACTGA AGTGATTGCT AATACACGAA AAGGCAAGCA AGTTATGAAT GTGAAATTTC CTGATAAGGT AAAACTTTGT GTTCCAGTAA AAGGTGATCA TGTTGCAGTG GTTGGTGAAA ACCGTAAAAT GCTCATTTTT GCCATTGAAC AAATACCAGA AATGAGTCGT GGTAAAGGTG TTCGTTTACA ACGTTACAAA CAAGGGGGAG TTGTTGATGT CAAAACTTTT AATTTATCAG AAGGTTTAAG TTGGCAAGAT ACAGCTGAAC GTTGCTTTAA CCGCCAAGCG AGTGATCTCA TTGAGTGGAT GGGAGTACGT TCTGGGGTAG GGCGTGTGGT TCCGAAAGGC TTTCCAAAAT CAGGGAAATT TACCAAT
Details for BaheA.00157.a.A1.GE25186
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDKMNLPF DKEHIESIEL RSALQERYLA YALSTITHRA LPDVRDGLKP VHRRIVHAMR LLKLNPGHSY AKCARIVGDV MGKFHPHGDA SIYDALVRLA QNFAVRYPLV DGQGNFGNID GDNAAAMRYT EARMTEVAAL LLEGINENAI DFRLTYNEED EEPIVLPGAF PNLLANGSSG IAVGMATSIP PHNVSELCDA ALHLIAHPNA RDEELNHFVL GPDFPTGGIL VEPKKSIEES YRVGRGFFRL RSRWHXETGN RGXYVLVVTE IPYQVXXSXL IE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg ataaaatgaa tttacctttt gataaagaac atattgagtc aatagaatta cgttctgctt tacaagagcg ttatttggct tatgcgcttt ctactattac gcaccgtgcg ttgcctgatg tgcgtgatgg tttgaaacct gtgcataggc gtattgttca cgctatgcgt ttactcaaac ttaatccagg acattcttat gcgaaatgcg caaggattgt tggggatgtc atgggaaaat ttcatccaca tggtgatgca tctatttatg atgcattagt gcgcttggct cagaattttg ctgtccgtta tccattggtt gatggacaag gcaattttgg gaatattgat ggtgataatg ctgctgctat gcgttatacg gaagcacgca tgactgaagt agctgcattg ctgcttgaag gaattaatga aaacgctatt gattttcgtt taacctataa tgaggaagat gaagaaccga tcgttttacc tggagctttt cctaatcttt tagcaaatgg ttcatcaggt attgctgttg gtatggcaac atctattcca ccccataatg tttctgaact ttgtgatgcg gcgcttcatt tgattgcgca tcccaatgcc cgtgatgagg aattaaatca ctttgtgctt ggacctgatt ttccnacagg tggcatttta gttgaaccta aaaagagtat tgaggaatct tatcgtgttg ggcgtggttt ttttcggttg cgttcgcgtt ggcatcnnga aaccggtaac cgtggttnnt atgtgcttgt tgtgactgaa attccctatc aagttnaaaa ntcacnactt attgaaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDKMNLPF DKEHIESIEL RSALQERYLA YALSTITHRA LPDVRDGLKP VHRRIVHAMR LLKLNPGHSY AKCARIVGDV MGKFHPHGDA SIYDALVRLA QNFAVRYPLV DGQGNFGNID GDNAAAMRYT EARMTEVAAL LLEGINENAI DFRLTYNEED EEPIVLPGAF PNLLANGSSG IAVGMATSIP PHNVSELCDA ALHLIAHPNA RDEELNHFVL GPDFPTGGIL VEPKKSIEES YRVGRGFFRL RSRWHQETGN RGSYVLVVTE IPYQVQKSRL IEKTAELLLA RRLPMLEDIR DESAEDIRIV LIPKNRTVDP EILMESLFKL TDFEIRFPLN LNVLSFGKVP NVLSLRESLK QWLEHRQEVL IRRSRYQLDE IERRLEILHG YLITYLNLDE VIQIIREEDE PKKKLIDRFE LTENQAEAIL NMRLRSLRKL EEFEIRKEFE TLRAEKEKLQ NLLDSRTKQW KIISEEIIKV RKVFGPHTLL GKRRTTFEEA PRHDFDDIYQ AMIEKEPVTI VISEKGWMRA LKGHLSDYNA LSFKEGDCLK LAFPAFTTDK IVVISTGGKF FAIAANSLPG GRGHGEPIRI LVDMDDEHDL LTAFVHHTQE KLLLVSSHGN GFIVPATEVI ANTRKGKQVM NVKFPDKVKL CVPVKGDHVA VVGENRKMLI FAIEQIPEMS RGKGVRLQRY KQGGVVDVKT FNLSEGLSWQ DTAERCFNRQ ASDLIEWMGV RSGVGRVVPK GFPKSGKFTN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgataaa atgaatttac cttttgataa agaacatatt gagtcaatag aattacgttc tgctttacaa gagcgttatt tggcttatgc gctttctact attacgcacc gtgcgttgcc tgatgtgcgt gatggtttga aacctgtgca taggcgtatt gttcacgcta tgcgtttact caaacttaat ccaggacatt cttatgcgaa atgcgcaagg attgttgggg atgtcatggg aaaatttcat ccacatggtg atgcatctat ttatgatgca ttagtgcgct tggctcagaa ttttgctgtc cgttatccat tggttgatgg acaaggcaat tttgggaata ttgatggtga taatgctgct gctatgcgtt atacggaagc acgcatgact gaagtagctg cattgctgct tgaaggaatt aatgaaaacg ctattgattt tcgtttaacc tataatgagg aagatgaaga accgatcgtt ttacctggag cttttcctaa tcttttagca aatggttcat caggtattgc tgttggtatg gcaacatcta ttccacccca taatgtttct gaactttgtg atgcggcgct tcatttgatt gcgcatccca atgcccgtga tgaggaatta aatcactttg tgcttggacc tgattttcca acaggtggca ttttagttga acctaaaaag agtattgagg aatcttatcg tgttgggcgt ggtttttttc ggttgcgttc gcgttggcat caggaaaccg gtaaccgtgg ttcttatgtg cttgttgtga ctgaaattcc ctatcaagtt caaaagtcac gacttattga aaaaacagca gaattattgc ttgcacggcg gttaccaatg cttgaagata ttcgtgatga gtcagcagaa gatattcgca ttgtacttat tcctaaaaat cgtaccgtcg atcctgagat tcttatggag tctcttttta aattgactga ttttgaaata agatttcctc tcaatcttaa tgttttgtct tttggaaaag taccgaatgt tctttctttg cgggaaagtt taaaacaatg gcttgaacat cgtcaagagg tgcttattcg ccgttctcgt tatcagcttg acgaaattga acgacggttg gaaatcctcc atggatatct tatcacatat ttgaatcttg atgaggtgat ccagattatt cgggaagagg atgaaccaaa aaaaaagctt atcgatcgtt ttgaattaac agaaaatcaa gctgaagcta ttcttaatat gcgcttgcgt tctttacgta aacttgagga atttgaaatt cgtaaagaat ttgaaacctt gagggctgaa aaagaaaaac tgcaaaattt gttagattct cggacaaagc aatggaaaat aatttcagag gaaatcataa aagttcgtaa agtttttggt cctcatactc tcttagggaa aaggcgtaca acatttgaag aagcaccaag acatgatttt gatgatattt atcaggcaat gattgaaaaa gaaccagtta ctattgttat ttctgaaaag ggttggatgc gtgctttgaa ggggcatttg agtgattaca atgcactttc tttcaaagaa ggggattgtt tgaaattagc atttccagcg tttactacag ataaaattgt ggtgataagt actggcggaa agttttttgc tattgctgca aatagtttgc cgggaggacg ggggcatggg gaacccattc gtattttagt tgatatggat gacgagcatg atctcctgac agcttttgtg catcatacac aggaaaaact tctcttagtt tcatctcatg gaaatggttt tattgtacct gcaactgaag tgattgctaa tacacgaaaa ggcaagcaag ttatgaatgt gaaatttcct gataaggtaa aactttgtgt tccagtaaaa ggtgatcatg ttgcagtggt tggtgaaaac cgtaaaatgc tcatttttgc cattgaacaa ataccagaaa tgagtcgtgg taaaggtgtt cgtttacaac gttacaaaca agggggagtt gttgatgtca aaacttttaa tttatcagaa ggtttaagtt ggcaagatac agctgaacgt tgctttaacc gccaagcgag tgatctcatt gagtggatgg gagtacgttc tggggtaggg cgtgtggttc cgaaaggctt tccaaaatca gggaaattta ccaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BaheA.00157.a.A1.PS00084
PURIFICATION DATe: 10/7/2008
CONCENTRATION: 5.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 39
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDKMNLPF DKEHIESIEL RSALQERYLA YALSTITHRA LPDVRDGLKP VHRRIVHAMR LLKLNPGHSY AKCARIVGDV MGKFHPHGDA SIYDALVRLA QNFAVRYPLV DGQGNFGNID GDNAAAMRYT EARMTEVAAL LLEGINENAI DFRLTYNEED EEPIVLPGAF PNLLANGSSG IAVGMATSIP PHNVSELCDA ALHLIAHPNA RDEELNHFVL GPDFPTGGIL VEPKKSIEES YRVGRGFFRL RSRWHXETGN RGXYVLVVTE IPYQVXXSXL IE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg ataaaatgaa tttacctttt gataaagaac atattgagtc aatagaatta cgttctgctt tacaagagcg ttatttggct tatgcgcttt ctactattac gcaccgtgcg ttgcctgatg tgcgtgatgg tttgaaacct gtgcataggc gtattgttca cgctatgcgt ttactcaaac ttaatccagg acattcttat gcgaaatgcg caaggattgt tggggatgtc atgggaaaat ttcatccaca tggtgatgca tctatttatg atgcattagt gcgcttggct cagaattttg ctgtccgtta tccattggtt gatggacaag gcaattttgg gaatattgat ggtgataatg ctgctgctat gcgttatacg gaagcacgca tgactgaagt agctgcattg ctgcttgaag gaattaatga aaacgctatt gattttcgtt taacctataa tgaggaagat gaagaaccga tcgttttacc tggagctttt cctaatcttt tagcaaatgg ttcatcaggt attgctgttg gtatggcaac atctattcca ccccataatg tttctgaact ttgtgatgcg gcgcttcatt tgattgcgca tcccaatgcc cgtgatgagg aattaaatca ctttgtgctt ggacctgatt ttccnacagg tggcatttta gttgaaccta aaaagagtat tgaggaatct tatcgtgttg ggcgtggttt ttttcggttg cgttcgcgtt ggcatcnnga aaccggtaac cgtggttnnt atgtgcttgt tgtgactgaa attccctatc aagttnaaaa ntcacnactt attgaaaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDKMNLPF DKEHIESIEL RSALQERYLA YALSTITHRA LPDVRDGLKP VHRRIVHAMR LLKLNPGHSY AKCARIVGDV MGKFHPHGDA SIYDALVRLA QNFAVRYPLV DGQGNFGNID GDNAAAMRYT EARMTEVAAL LLEGINENAI DFRLTYNEED EEPIVLPGAF PNLLANGSSG IAVGMATSIP PHNVSELCDA ALHLIAHPNA RDEELNHFVL GPDFPTGGIL VEPKKSIEES YRVGRGFFRL RSRWHQETGN RGSYVLVVTE IPYQVQKSRL IEKTAELLLA RRLPMLEDIR DESAEDIRIV LIPKNRTVDP EILMESLFKL TDFEIRFPLN LNVLSFGKVP NVLSLRESLK QWLEHRQEVL IRRSRYQLDE IERRLEILHG YLITYLNLDE VIQIIREEDE PKKKLIDRFE LTENQAEAIL NMRLRSLRKL EEFEIRKEFE TLRAEKEKLQ NLLDSRTKQW KIISEEIIKV RKVFGPHTLL GKRRTTFEEA PRHDFDDIYQ AMIEKEPVTI VISEKGWMRA LKGHLSDYNA LSFKEGDCLK LAFPAFTTDK IVVISTGGKF FAIAANSLPG GRGHGEPIRI LVDMDDEHDL LTAFVHHTQE KLLLVSSHGN GFIVPATEVI ANTRKGKQVM NVKFPDKVKL CVPVKGDHVA VVGENRKMLI FAIEQIPEMS RGKGVRLQRY KQGGVVDVKT FNLSEGLSWQ DTAERCFNRQ ASDLIEWMGV RSGVGRVVPK GFPKSGKFTN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGATAAA ATGAATTTAC CTTTTGATAA AGAACATATT GAGTCAATAG AATTACGTTC TGCTTTACAA GAGCGTTATT TGGCTTATGC GCTTTCTACT ATTACGCACC GTGCGTTGCC TGATGTGCGT GATGGTTTGA AACCTGTGCA TAGGCGTATT GTTCACGCTA TGCGTTTACT CAAACTTAAT CCAGGACATT CTTATGCGAA ATGCGCAAGG ATTGTTGGGG ATGTCATGGG AAAATTTCAT CCACATGGTG ATGCATCTAT TTATGATGCA TTAGTGCGCT TGGCTCAGAA TTTTGCTGTC CGTTATCCAT TGGTTGATGG ACAAGGCAAT TTTGGGAATA TTGATGGTGA TAATGCTGCT GCTATGCGTT ATACGGAAGC ACGCATGACT GAAGTAGCTG CATTGCTGCT TGAAGGAATT AATGAAAACG CTATTGATTT TCGTTTAACC TATAATGAGG AAGATGAAGA ACCGATCGTT TTACCTGGAG CTTTTCCTAA TCTTTTAGCA AATGGTTCAT CAGGTATTGC TGTTGGTATG GCAACATCTA TTCCACCCCA TAATGTTTCT GAACTTTGTG ATGCGGCGCT TCATTTGATT GCGCATCCCA ATGCCCGTGA TGAGGAATTA AATCACTTTG TGCTTGGACC TGATTTTCCA ACAGGTGGCA TTTTAGTTGA ACCTAAAAAG AGTATTGAGG AATCTTATCG TGTTGGGCGT GGTTTTTTTC GGTTGCGTTC GCGTTGGCAT CAGGAAACCG GTAACCGTGG TTCTTATGTG CTTGTTGTGA CTGAAATTCC CTATCAAGTT CAAAAGTCAC GACTTATTGA AAAAACAGCA GAATTATTGC TTGCACGGCG GTTACCAATG CTTGAAGATA TTCGTGATGA GTCAGCAGAA GATATTCGCA TTGTACTTAT TCCTAAAAAT CGTACCGTCG ATCCTGAGAT TCTTATGGAG TCTCTTTTTA AATTGACTGA TTTTGAAATA AGATTTCCTC TCAATCTTAA TGTTTTGTCT TTTGGAAAAG TACCGAATGT TCTTTCTTTG CGGGAAAGTT TAAAACAATG GCTTGAACAT CGTCAAGAGG TGCTTATTCG CCGTTCTCGT TATCAGCTTG ACGAAATTGA ACGACGGTTG GAAATCCTCC ATGGATATCT TATCACATAT TTGAATCTTG ATGAGGTGAT CCAGATTATT CGGGAAGAGG ATGAACCAAA AAAAAAGCTT ATCGATCGTT TTGAATTAAC AGAAAATCAA GCTGAAGCTA TTCTTAATAT GCGCTTGCGT TCTTTACGTA AACTTGAGGA ATTTGAAATT CGTAAAGAAT TTGAAACCTT GAGGGCTGAA AAAGAAAAAC TGCAAAATTT GTTAGATTCT CGGACAAAGC AATGGAAAAT AATTTCAGAG GAAATCATAA AAGTTCGTAA AGTTTTTGGT CCTCATACTC TCTTAGGGAA AAGGCGTACA ACATTTGAAG AAGCACCAAG ACATGATTTT GATGATATTT ATCAGGCAAT GATTGAAAAA GAACCAGTTA CTATTGTTAT TTCTGAAAAG GGTTGGATGC GTGCTTTGAA GGGGCATTTG AGTGATTACA ATGCACTTTC TTTCAAAGAA GGGGATTGTT TGAAATTAGC ATTTCCAGCG TTTACTACAG ATAAAATTGT GGTGATAAGT ACTGGCGGAA AGTTTTTTGC TATTGCTGCA AATAGTTTGC CGGGAGGACG GGGGCATGGG GAACCCATTC GTATTTTAGT TGATATGGAT GACGAGCATG ATCTCCTGAC AGCTTTTGTG CATCATACAC AGGAAAAACT TCTCTTAGTT TCATCTCATG GAAATGGTTT TATTGTACCT GCAACTGAAG TGATTGCTAA TACACGAAAA GGCAAGCAAG TTATGAATGT GAAATTTCCT GATAAGGTAA AACTTTGTGT TCCAGTAAAA GGTGATCATG TTGCAGTGGT TGGTGAAAAC CGTAAAATGC TCATTTTTGC CATTGAACAA ATACCAGAAA TGAGTCGTGG TAAAGGTGTT CGTTTACAAC GTTACAAACA AGGGGGAGTT GTTGATGTCA AAACTTTTAA TTTATCAGAA GGTTTAAGTT GGCAAGATAC AGCTGAACGT TGCTTTAACC GCCAAGCGAG TGATCTCATT GAGTGGATGG GAGTACGTTC TGGGGTAGGG CGTGTGGTTC CGAAAGGCTT TCCAAAATCA GGGAAATTTA CCAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA