BaheA.00154.a

chorismate synthase

CENTER ID:	BaheA.00154.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00154.a.A1.GE25185	Full length( BaheA.00154.a )		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128304
PATRIC ID:	fig\|283166.5.peg.473
RefSeq:	YP_033270.1
UniProt:	Q6G4D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSHNTFGHLF RVTTWGESHG EALGCVIDGC PPGITFTLSD IQSYLNKRKP GQSKYTTQRR ELDQVEILSG VIVQDDGITF VTTGTPISLL IRNMDQRSKD YSAIVHQYRP GHADYTYDVK YGIRDFRGGG RASARETAAR VAAGAVARKI IPGVIVRGAV IAIGPHTINR NRWNWSEVDN NPFFTPDAEM VQVFSDYIDK VHKNGSSVGA VVEIVAENIP AGLGAPIYAK LDQDIASLLM SINAVKGVEI GDGFAAARLT GEENADEMRM GSDGKPLFLS NHAGGIVGGI SSGQPIIARF AVKPTSSILI PRRSIDVDGN DVNVITKGRH DPCVGIRAVP VGEAMVACAL ADHYLRHRGQ VGCFKR

NT Sequence

ATGTCACATA ATACATTTGG TCATCTATTT CGTGTAACAA CATGGGGTGA AAGTCATGGT GAGGCTCTTG GTTGTGTCAT TGATGGTTGT CCTCCAGGCA TTACTTTTAC TCTTTCAGAT ATTCAATCTT ATCTTAATAA ACGTAAACCG GGACAATCCA AATACACGAC GCAGCGCCGT GAGTTGGATC AAGTAGAGAT ACTTTCAGGA GTTATTGTTC AAGACGATGG GATAACATTT GTGACAACAG GAACACCAAT TTCCCTGTTG ATTCGAAATA TGGATCAGCG TTCTAAGGAT TATAGCGCAA TAGTTCATCA GTATCGTCCA GGGCATGCAG ATTATACGTA TGATGTTAAG TATGGCATTC GTGATTTTCG AGGTGGCGGA CGTGCTTCAG CACGTGAGAC GGCAGCACGT GTTGCAGCAG GTGCTGTTGC TCGTAAAATT ATTCCTGGTG TGATTGTACG GGGAGCTGTG ATAGCAATTG GTCCTCATAC GATTAATCGC AATCGTTGGA ACTGGTCAGA GGTTGATAAC AATCCTTTTT TTACGCCTGA TGCAGAGATG GTACAGGTTT TTAGTGATTA TATCGATAAG GTTCATAAAA ATGGTTCATC TGTTGGTGCT GTTGTCGAGA TTGTTGCAGA AAATATTCCA GCAGGTTTGG GAGCACCTAT TTACGCAAAA CTTGATCAGG ATATCGCATC ATTACTTATG TCGATTAATG CGGTGAAAGG TGTCGAAATA GGTGATGGTT TTGCAGCAGC ACGCTTGACG GGGGAAGAAA ATGCAGATGA AATGCGGATG GGGAGTGATG GAAAACCGCT CTTTTTATCC AATCATGCTG GTGGTATTGT AGGCGGAATA TCGAGTGGAC AGCCGATTAT TGCACGTTTT GCAGTAAAGC CTACTTCATC AATTTTGATT CCTCGTCGTT CAATTGATGT TGATGGTAAT GATGTAAATG TTATAACGAA GGGGCGTCAT GATCCATGTG TTGGGATTCG TGCTGTTCCT GTTGGTGAAG CAATGGTAGC TTGTGCTCTT GCGGATCATT ATCTCAGGCA TCGCGGCCAG GTAGGGTGCT TCAAAAGG

Details for BaheA.00154.a.A1.GE25185

HARVESTED ON:	5/6/2008
SEQUENCED ON:	7/29/2008
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSHNTFGHL FRVTTWGESH GEALGCVIDG CPPGITFTLS DIQSYLNKRK PGQSKYTTQR RELDQVEILS GVIVQDDGIT FVTTGTPISL LIRNMDQRSK DYSAIVHQYR PGHADYTYDV KYGIRDFRGG GRASARETAA RVAAGAVARK IIPGVIVRGA VIAIGPHTIN RNRWNWSEVD NNPFFTPDAE MVQVFSDYID KVHKNGSSVG AVVEIVAENI PAGLGAPIYA KLDQDIASLL MSINAVKGVE IGDGFAAARL TGEENADEMR MGSDGKPLFL SNHAGGIVGG ISSGQPIIAR FAVKPTSSIL IPRRSIDVDG NDVNVITKGR HDPCVGIRAV PVGEAMVACA LADHYLRHRG QVGCF

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac ataatacatt tggtcatcta tttcgtgtaa caacatgggg tgaaagtcat ggtgaggctc ttggttgtgt cattgatggt tgtcctccag gcattacttt tactctttca gatattcaat cttatcttaa taaacgtaaa ccgggacaat ccaaatacac gacgcagcgc cgtgagttgg atcaagtaga gatactttca ggagttattg ttcaagacga tgggataaca tttgtgacaa caggaacacc aatttccctg ttgattcgaa atatggatca gcgttctaag gattatagcg caatagttca tcagtatcgt ccagggcatg cagattatac gtatgatgtt aagtatggca ttcgtgattt tcgaggtggc ggacgtgctt cagcacgtga gacggcagca cgtgttgcag caggtgctgt tgctcgtaaa attattcctg gtgtgattgt acggggagct gtgatagcaa ttggtcctca tacgattaat cgcaatcgtt ggaactggtc agaggttgat aacaatcctt tttttacgcc tgatgcagag atggtacagg tttttagtga ttatatcgat aaggttcata aaaatggttc atctgttggt gctgttgtcg agattgttgc agaaaatatt ccagcaggtt tgggagcacc tatttacgca aaacttgatc aggatatcgc atcattactt atgtcgatta atgcggtgaa aggtgtcgaa ataggtgatg gttttgcagc agcacgcttg acgggggaag aaaatgcaga tgaaatgcgg atggggagtg atggaaaacc gctcttttta tccaatcatg ctggtggtat tgtaggcgga atatcgagtg gacagccgat tattgcacgt tttgcagtaa agcctacttc atcaattttg attcctcgtc gttcaattga tgttgatggt aatgatgtaa atgttataac gaaggggcgt catgatccat gtgttgggat tcgtgctgtt cctgttggtg aagcaatggt agcttgtgct cttgcggatc attatctcag gcatcgcggc caggtagggt gcttca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcacataat acatttggtc atctatttcg tgtaacaaca tggggtgaaa gtcatggtga ggctcttggt tgtgtcattg atggttgtcc tccaggcatt acttttactc tttcagatat tcaatcttat cttaataaac gtaaaccggg acaatccaaa tacacgacgc agcgccgtga gttggatcaa gtagagatac tttcaggagt tattgttcaa gacgatggga taacatttgt gacaacagga acaccaattt ccctgttgat tcgaaatatg gatcagcgtt ctaaggatta tagcgcaata gttcatcagt atcgtccagg gcatgcagat tatacgtatg atgttaagta tggcattcgt gattttcgag gtggcggacg tgcttcagca cgtgagacgg cagcacgtgt tgcagcaggt gctgttgctc gtaaaattat tcctggtgtg attgtacggg gagctgtgat agcaattggt cctcatacga ttaatcgcaa tcgttggaac tggtcagagg ttgataacaa tccttttttt acgcctgatg cagagatggt acaggttttt agtgattata tcgataaggt tcataaaaat ggttcatctg ttggtgctgt tgtcgagatt gttgcagaaa atattccagc aggtttggga gcacctattt acgcaaaact tgatcaggat atcgcatcat tacttatgtc gattaatgcg gtgaaaggtg tcgaaatagg tgatggtttt gcagcagcac gcttgacggg ggaagaaaat gcagatgaaa tgcggatggg gagtgatgga aaaccgctct ttttatccaa tcatgctggt ggtattgtag gcggaatatc gagtggacag ccgattattg cacgttttgc agtaaagcct acttcatcaa ttttgattcc tcgtcgttca attgatgttg atggtaatga tgtaaatgtt ataacgaagg ggcgtcatga tccatgtgtt gggattcgtg ctgttcctgt tggtgaagca atggtagctt gtgctcttgc ggatcattat ctcaggcatc gcggccaggt agggtgcttc aaaaggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa