BaheA.00148.a

site-specific tyrosine recombinase XerC

CENTER ID:	BaheA.00148.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00148.a.B1.GE25278	Full length( BaheA.00148.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00148.a.B1.PW25316	Full length( BaheA.00148.a )		1	326

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_137884
PATRIC ID:	fig\|283166.5.peg.1841
RefSeq:	YP_034281.1
UniProt:	A0A0H3M721

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLPKKDSRKD KNFSLIPAAP PVLAARKNWL DNLLGTRRMA TYTAQAYERD TRQFLFFLCQ HLGHTPTFKD LANLRVIDLR AYLAYRRKHD ISARSLSRGM AGIRSFFNYL SREGIASIPA AKLVRTPKHP KSLPKPLNIK SALHLVKQEN QQENEPWIIA RNTAVLMLLY GCGMRISEAL SLTPEQFSDP QKTSLFVTGK GGKTRLVPLI KVVYEAIQNY LKCCPYPLLD NQPMFRGARG GPLQPAIIQK TVRNLRAYLG LPETATPHTL RHSFATHLLS RGGDLRTIQE LLGHACLSTT QVYTHVDTKH LLEIYQKAHP RSLKDF

NT Sequence

ATGCTACCAA AAAAGGACAG TAGAAAAGAC AAAAATTTTT CTCTGATTCC AGCCGCCCCT CCTGTTTTAG CTGCAAGGAA GAATTGGCTG GATAATCTTT TAGGAACACG TCGTATGGCA ACATATACAG CACAAGCCTA TGAGCGTGAT ACACGACAGT TTTTGTTTTT TCTCTGTCAA CATCTCGGAC ATACACCAAC CTTTAAGGAT CTCGCCAATT TGCGTGTCAT TGATTTGCGT GCTTATCTAG CTTACCGCCG CAAACACGAC ATAAGTGCTC GCTCTTTAAG CCGTGGAATG GCGGGTATAC GTTCTTTTTT CAACTACCTA TCCCGTGAAG GAATCGCCAG CATTCCTGCT GCTAAACTTG TCCGAACCCC CAAACACCCA AAATCGCTAC CAAAACCTTT GAACATAAAA TCAGCCCTGC ATTTAGTTAA ACAAGAAAAT CAACAGGAAA ATGAACCATG GATCATCGCC CGTAACACTG CTGTTTTAAT GCTTCTTTAT GGCTGTGGAA TGCGGATATC AGAAGCTTTA AGCCTCACAC CAGAACAATT TTCTGATCCG CAAAAAACAA GTTTATTCGT GACTGGGAAA GGAGGGAAAA CACGTCTTGT CCCCCTTATT AAAGTGGTTT ACGAAGCAAT ACAAAATTAT CTTAAATGCT GCCCATATCC TTTATTGGAC AATCAACCAA TGTTCCGCGG TGCGCGAGGT GGTCCCTTGC AGCCAGCTAT TATTCAAAAA ACTGTGCGAA ACTTACGTGC ATACCTTGGT TTACCAGAAA CAGCCACTCC CCATACACTG CGCCATTCTT TTGCCACCCA TCTTTTATCA CGAGGAGGAG ATTTACGTAC CATTCAAGAG CTCCTAGGTC ACGCTTGTCT TTCCACCACC CAAGTCTACA CCCATGTAGA TACCAAGCAC TTGTTAGAAA TTTATCAAAA AGCCCACCCT CGTAGCTTGA AAGACTTT

Details for BaheA.00148.a.B1.GE25278

HARVESTED ON:	6/3/2008
SEQUENCED ON:	7/20/2010
EXPECTED MW:	37kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHML PKKDSRKDKN FSLIPAAPPV LAARKNWLDN LLGTRRMATY TAQAYERDTR QFLFFLCQHL GHTPTFKDLA NLRVIDLRAY LAYRRKHDIS ARSLSRGMAG IRSFFNYLSR EGIASIPAAK LVRTPKHPKS LPKPLNIKSA LHLVKQENQQ ENEPWIIARN TAVLMLLYGC GMRISEALSL TPEQFSDPQK TSLFVTGKGG KTRLVPLIKV VYEAIQNYLK CCPYPLLDNQ PMFRGARGGP LQPAIIQKTV RNLRAYLGLP ETATPHTLRH SFATHLLSRG GDLRTIQELL GHACLSTTQV YXXVDXKHLX

Validated NT Sequence

tttaagaagg aganatacca tggctcacca ccaccaccac catatgctac caaaaaagga cagtagaaaa gacaaaaatt tttctctgat tccagccgcc cctcctgttt tagctgcaag gaagaattgg ctggataatc ttttaggaac acgtcgtatg gcaacatata cagcacaagc ctatgagcgt gatacacgac agtttttgtt ttttctctgt caacatctcg gacatacacc aacctttaag gatctcgcca atttgcgtgt cattgatttg cgtgcttatc tagcttaccg ccgcaaacac gacataagtg ctcgctcttt aagccgtgga atggcgggta tacgttcttt tttcaactac ctatcccgtg aaggaatcgc cagcattcct gctgctaaac ttgtccgaac ccccaaacac ccaaaatcgc taccaaaacc tttgaacata aaatcagccc tgcatttagt taaacaagaa aatcaacagg aaaatgaacc atggatcatc gcccgtaaca ctgctgtttt aatgcttctt tatggctgtg gaatgcggat atcagaagct ttaagcctca caccagaaca attttctgat ccgcaaaaaa caagtttatt cgtgactggg aaaggaggga aaacacgtct tgtccccctt attaaagtgg tttacgaagc aatacaaaat tatcttaaat gctgcccata tcctttattg gacaatcaac caatgttccg cggtgcgcga ggtggtccct tgcagccagc tattattcaa aaaactgtgc gaaacttacg tgcatacctt ggtttaccag aaacagccac tccccataca ctgcgccatt cttttgccac ccatctttta tcacgaggag gagatttacg taccattcaa gagctcctag gtcacgcttg tctttccacc acccaagtct acncccntgt agatnccaag cacttgntag aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac caaaaaagga cagtagaaaa gacaaaaatt tttctctgat tccagccgcc cctcctgttt tagctgcaag gaagaattgg ctggataatc ttttaggaac acgtcgtatg gcaacatata cagcacaagc ctatgagcgt gatacacgac agtttttgtt ttttctctgt caacatctcg gacatacacc aacctttaag gatctcgcca atttgcgtgt cattgatttg cgtgcttatc tagcttaccg ccgcaaacac gacataagtg ctcgctcttt aagccgtgga atggcgggta tacgttcttt tttcaactac ctatcccgtg aaggaatcgc cagcattcct gctgctaaac ttgtccgaac ccccaaacac ccaaaatcgc taccaaaacc tttgaacata aaatcagccc tgcatttagt taaacaagaa aatcaacagg aaaatgaacc atggatcatc gcccgtaaca ctgctgtttt aatgcttctt tatggctgtg gaatgcggat atcagaagct ttaagcctca caccagaaca attttctgat ccgcaaaaaa caagtttatt cgtgactggg aaaggaggga aaacacgtct tgtccccctt attaaagtgg tttacgaagc aatacaaaat tatcttaaat gctgcccata tcctttattg gacaatcaac caatgttccg cggtgcgcga ggtggtccct tgcagccagc tattattcaa aaaactgtgc gaaacttacg tgcatacctt ggtttaccag aaacagccac tccccataca ctgcgccatt cttttgccac ccatctttta tcacgaggag gagatttacg taccattcaa gagctcctag gtcacgcttg tctttccacc acccaagtct acacccatgt agataccaag cacttgttag aaatttatca aaaagcccac cctcgtagct tgaaagactt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BaheA.00148.a.B1.PW25316

PURIFICATION DATe:	1/19/2010
CONCENTRATION:	4.71mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	98
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAC CAAAAAAGGA CAGTAGAAAA GACAAAAATT TTTCTCTGAT TCCAGCCGCC CCTCCTGTTT TAGCTGCAAG GAAGAATTGG CTGGATAATC TTTTAGGAAC ACGTCGTATG GCAACATATA CAGCACAAGC CTATGAGCGT GATACACGAC AGTTTTTGTT TTTTCTCTGT CAACATCTCG GACATACACC AACCTTTAAG GATCTCGCCA ATTTGCGTGT CATTGATTTG CGTGCTTATC TAGCTTACCG CCGCAAACAC GACATAAGTG CTCGCTCTTT AAGCCGTGGA ATGGCGGGTA TACGTTCTTT TTTCAACTAC CTATCCCGTG AAGGAATCGC CAGCATTCCT GCTGCTAAAC TTGTCCGAAC CCCCAAACAC CCAAAATCGC TACCAAAACC TTTGAACATA AAATCAGCCC TGCATTTAGT TAAACAAGAA AATCAACAGG AAAATGAACC ATGGATCATC GCCCGTAACA CTGCTGTTTT AATGCTTCTT TATGGCTGTG GAATGCGGAT ATCAGAAGCT TTAAGCCTCA CACCAGAACA ATTTTCTGAT CCGCAAAAAA CAAGTTTATT CGTGACTGGG AAAGGAGGGA AAACACGTCT TGTCCCCCTT ATTAAAGTGG TTTACGAAGC AATACAAAAT TATCTTAAAT GCTGCCCATA TCCTTTATTG GACAATCAAC CAATGTTCCG CGGTGCGCGA GGTGGTCCCT TGCAGCCAGC TATTATTCAA AAAACTGTGC GAAACTTACG TGCATACCTT GGTTTACCAG AAACAGCCAC TCCCCATACA CTGCGCCATT CTTTTGCCAC CCATCTTTTA TCACGAGGAG GAGATTTACG TACCATTCAA GAGCTCCTAG GTCACGCTTG TCTTTCCACC ACCCAAGTCT ACACCCATGT AGATACCAAG CACTTGTTAG AAATTTATCA AAAAGCCCAC CCTCGTAGCT TGAAAGACTT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT