BaheA.00133.a

cysteinyl-tRNA synthetase

CENTER ID: BaheA.00133.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00133.a.A1.GE25180 Full length( BaheA.00133.a ) 1 503
BaheA.00133.a.B1.GE25276 Full length( BaheA.00133.a ) 1 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00133.a.A1.PS00074 Full length( BaheA.00133.a ) 1 503
BaheA.00133.a.B1.PS00096 Full length( BaheA.00133.a ) 1 503
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126755
PATRIC ID: fig|283166.5.peg.564
RefSeq: YP_033354.1
UniProt: Q6G5G9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRELRFYNTL TRKKENFIPI DATRVRLYVC GPTVYDYAHI GNARPVIVFD ILFRLLRHVY GNEHVIYARN ITDVDDKINA RAAYEYPELA LNDAIRQLTQ LTYTQFQQDT MALGCLPPTI EPRATDHLEE MRSLIERLLE KGHAYKAENH VLFSVSSIRN DPHYGSFAKR SLDEMRAGAR VDVASYKREE MDFVLWKPSA EGEPGWTSPA GIPVLGRPGW HIECSAMSMA KLLAPYGGGL TCDDPTENVF DIHGGGLDLI FPHHENEIAQ SCSAFGTERM ANLWMHNGFL QVEGKKMSKS LSNFITIRSI LESDFFEFNG VLTNEMKQNW AGLSARFSML QTHYREPLNW TAQRLMQSSS ELYRWYELLR CEKTEMEKKN EVIDDSLINA LSDDLNTPKA LTLLRKFYKR GNAVSLASGM NFFGLLRQEW VRDGKCPLFM RKTSLDEKFI DQRIAERLRL IHNKEWTAAD TIRDKLAAEG IVLKDIKDPQ TGERTTMWEI KRF
NT Sequence
ATGAGAGAGT TGCGATTTTA TAATACGCTT ACACGTAAAA AAGAGAATTT TATACCGATA GATGCAACAA GAGTCCGTCT TTATGTTTGT GGTCCGACGG TTTATGATTA TGCGCATATA GGTAACGCAC GTCCTGTGAT TGTTTTTGAT ATTTTATTTC GCTTATTGCG CCATGTTTAT GGCAATGAGC ATGTTATATA TGCTCGTAAT ATTACAGATG TGGATGATAA GATTAATGCA CGCGCAGCTT ATGAATATCC AGAACTAGCA CTAAACGACG CTATTCGCCA ATTAACGCAG CTTACATATA CTCAATTTCA ACAAGATACA ATGGCACTTG GTTGTCTACC GCCCACCATT GAACCGCGCG CAACTGATCA TTTAGAGGAG ATGCGTTCTT TAATTGAAAG ATTACTTGAA AAGGGGCATG CTTATAAAGC GGAAAATCAT GTTTTATTTT CGGTAAGCAG TATAAGAAAT GATCCCCATT ATGGATCATT TGCAAAACGC TCATTAGATG AAATGAGAGC GGGGGCACGT GTTGATGTTG CATCTTATAA AAGAGAAGAG ATGGATTTTG TTTTATGGAA GCCTTCTGCT GAGGGGGAAC CAGGTTGGAC ATCGCCGGCA GGAATTCCTG TTTTGGGGCG TCCGGGGTGG CATATTGAAT GTTCAGCAAT GTCCATGGCA AAGTTATTAG CACCCTATGG TGGTGGTTTA ACCTGCGATG ATCCAACAGA AAATGTTTTT GATATCCATG GTGGTGGACT TGATTTGATT TTTCCTCATC ATGAAAATGA GATTGCCCAA AGTTGCTCAG CTTTTGGGAC TGAACGAATG GCTAATCTTT GGATGCATAA CGGTTTTTTG CAAGTTGAAG GCAAGAAAAT GTCTAAAAGT CTTAGCAATT TCATCACCAT TCGTTCTATT TTAGAGAGTG ATTTTTTTGA ATTCAATGGT GTTTTAACAA ATGAAATGAA ACAAAATTGG GCTGGTTTAT CTGCCCGTTT TTCGATGTTA CAAACGCATT ATCGTGAACC GTTAAATTGG ACCGCTCAGC GTTTAATGCA GTCCAGTAGC GAATTGTATC GTTGGTATGA GTTGCTTCGT TGTGAAAAGA CGGAGATGGA GAAGAAGAAT GAGGTCATTG ATGACTCTTT GATAAACGCA CTCAGTGATG ACCTTAACAC TCCCAAAGCA CTTACTCTTT TACGAAAATT TTATAAACGA GGGAATGCTG TTTCTCTTGC AAGTGGGATG AATTTCTTTG GACTCTTACG GCAAGAGTGG GTTAGAGATG GAAAATGCCC ATTATTTATG AGAAAAACCT CTCTTGATGA AAAATTTATT GATCAGCGCA TTGCCGAAAG GTTACGACTT ATCCATAATA AAGAGTGGAC AGCTGCGGAT ACAATTCGTG ATAAGCTTGC AGCCGAGGGG ATTGTCTTAA AAGATATCAA GGATCCACAA ACTGGTGAAC GTACGACTAT GTGGGAAATA AAACGGTTC
Details for BaheA.00133.a.A1.GE25180
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRELRFYNT LTRKKENFIP IDATRVRLYV CGPTVYDYAH IGNARPVIVF DILFRLLRHV YGNEHVIYAR NITDVDDKIN ARAAYEYPEL ALNDAIRQLT QLTYTQFQQD TMALGCLPPT IEPRATDHLE EMRSLIERLL EKGHAYKAEN HVLFSVSSIR NDPHYGSFAK RSLDEMRAGA RVDVASYKRE EMDFVLWKPS AEGEPGWTSP AGIPVLGRPG WHIECSAMSM AKLLAPYGGG LTCDDPTENV FDIHGGGLDL IFPHHENEIA QSCSAFGTER MANLWMHNGF LQVEGKKMSK SLSNFITIRS ILESDFFEFN GVLTNEMKQN WAGLSARFSM LQTHYREPLN WTAQRLMQSS SELYRWYELL RCEKTEMEKK NEVIDDSLIN ALSDDLNTPK ALTLLRKFYK RGNAVSLASG MNFFGLLRQE WVRDGKCPLF MRKTSLDEKF IDQRIAERLR LIHNKEWTAA DTIRDKLAAE GIVLKDIKDP QTGERTTMWE IKRF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagag agttgcgatt ttataatacg cttacacgta aaaaagagaa ttttataccg atagatgcaa caagagtccg tctttatgtt tgtggtccga cggtttatga ttatgcgcat ataggtaacg cacgtcctgt gattgttttt gatattttat ttcgcttatt gcgccatgtt tatggcaatg agcatgttat atatgctcgt aatattacag atgtggatga taagattaat gcacgcgcag cttatgaata tccagaacta gcactaaacg acgctattcg ccaattaacg cagcttacat atactcaatt tcaacaagat acaatggcac ttggttgtct accgcccacc attgaaccgc gcgcaactga tcatttagag gagatgcgtt ctttaattga aagattactt gaaaaggggc atgcttataa agcggaaaat catgttttat tttcggtaag cagtataaga aatgatcccc attatggatc atttgcaaaa cgctcattag atgaaatgag agcgggggca cgtgttgatg ttgcatctta taaaagagaa gagatggatt ttgttttatg gaagccttct gctgaggggg aaccaggttg gacatcgccg gcaggaattc ctgttttggg gcgtccgggg tggcatattg aatgttcagc aatgtccatg gcaaagttat tagcacccta tggtggtggt ttaacctgcg atgatccaac agaaaatgtt tttgatatcc atggtggtgg acttgatttg atttttcctc atcatgaaaa tgagattgcc caaagttgct cagcttttgg gactgaacga atggctaatc tttggatgca taacggtttt ttgcaagttg aaggcaagaa aatgtctaaa agtcttagca atttcatcac cattcgttct attttagaga gtgatttttt tgaattcaat ggtgttttaa caaatgaaat gaaacaaaat tgggctggtt tatctgcccg tttttcgatg ttacaaacgc attatcgtga accgttaaat tggaccgctc agcgtttaat gcagtccagt agcgaattgt atcgttggta tgagttgctt cgttgtgaaa agacggagat ggagaagaag aatgaggtca ttgatgactc tttgataaac gcactcagtg atgaccttaa cactcccaaa gcacttactc ttttacgaaa attttataaa cgagggaatg ctgtttctct tgcaagtggg atgaatttct ttggactctt acggcaagag tgggttagag atggaaaatg cccattattt atgagaaaaa cctctcttga tgaaaaattt attgatcagc gcattgccga aaggttacga cttatccata ataaagagtg gacagctgcg gatacaattc gtgataagct tgcagccgag gggattgtct taaaagatat caaggatcca caaactggtg aacgtacgac tatgtgggaa ataaaacggt tct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRELRFYNT LTRKKENFIP IDATRVRLYV CGPTVYDYAH IGNARPVIVF DILFRLLRHV YGNEHVIYAR NITDVDDKIN ARAAYEYPEL ALNDAIRQLT QLTYTQFQQD TMALGCLPPT IEPRATDHLE EMRSLIERLL EKGHAYKAEN HVLFSVSSIR NDPHYGSFAK RSLDEMRAGA RVDVASYKRE EMDFVLWKPS AEGEPGWTSP AGIPVLGRPG WHIECSAMSM AKLLAPYGGG LTCDDPTENV FDIHGGGLDL IFPHHENEIA QSCSAFGTER MANLWMHNGF LQVEGKKMSK SLSNFITIRS ILESDFFEFN GVLTNEMKQN WAGLSARFSM LQTHYREPLN WTAQRLMQSS SELYRWYELL RCEKTEMEKK NEVIDDSLIN ALSDDLNTPK ALTLLRKFYK RGNAVSLASG MNFFGLLRQE WVRDGKCPLF MRKTSLDEKF IDQRIAERLR LIHNKEWTAA DTIRDKLAAE GIVLKDIKDP QTGERTTMWE IKRF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagagagttg cgattttata atacgcttac acgtaaaaaa gagaatttta taccgataga tgcaacaaga gtccgtcttt atgtttgtgg tccgacggtt tatgattatg cgcatatagg taacgcacgt cctgtgattg tttttgatat tttatttcgc ttattgcgcc atgtttatgg caatgagcat gttatatatg ctcgtaatat tacagatgtg gatgataaga ttaatgcacg cgcagcttat gaatatccag aactagcact aaacgacgct attcgccaat taacgcagct tacatatact caatttcaac aagatacaat ggcacttggt tgtctaccgc ccaccattga accgcgcgca actgatcatt tagaggagat gcgttcttta attgaaagat tacttgaaaa ggggcatgct tataaagcgg aaaatcatgt tttattttcg gtaagcagta taagaaatga tccccattat ggatcatttg caaaacgctc attagatgaa atgagagcgg gggcacgtgt tgatgttgca tcttataaaa gagaagagat ggattttgtt ttatggaagc cttctgctga gggggaacca ggttggacat cgccggcagg aattcctgtt ttggggcgtc cggggtggca tattgaatgt tcagcaatgt ccatggcaaa gttattagca ccctatggtg gtggtttaac ctgcgatgat ccaacagaaa atgtttttga tatccatggt ggtggacttg atttgatttt tcctcatcat gaaaatgaga ttgcccaaag ttgctcagct tttgggactg aacgaatggc taatctttgg atgcataacg gttttttgca agttgaaggc aagaaaatgt ctaaaagtct tagcaatttc atcaccattc gttctatttt agagagtgat ttttttgaat tcaatggtgt tttaacaaat gaaatgaaac aaaattgggc tggtttatct gcccgttttt cgatgttaca aacgcattat cgtgaaccgt taaattggac cgctcagcgt ttaatgcagt ccagtagcga attgtatcgt tggtatgagt tgcttcgttg tgaaaagacg gagatggaga agaagaatga ggtcattgat gactctttga taaacgcact cagtgatgac cttaacactc ccaaagcact tactctttta cgaaaatttt ataaacgagg gaatgctgtt tctcttgcaa gtgggatgaa tttctttgga ctcttacggc aagagtgggt tagagatgga aaatgcccat tatttatgag aaaaacctct cttgatgaaa aatttattga tcagcgcatt gccgaaaggt tacgacttat ccataataaa gagtggacag ctgcggatac aattcgtgat aagcttgcag ccgaggggat tgtcttaaaa gatatcaagg atccacaaac tggtgaacgt acgactatgt gggaaataaa acggttcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BaheA.00133.a.B1.GE25276
HARVESTED ON: 6/3/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Validated AA Sequence
MAHHHHHHMR ELRFYNTLTR KKENFIPIDA TRVRLYVCGP TVYDYAHIGN ARPVIVFDIL FRLLRHVYGN EHVIYARNIT DVDDKINARA AYEYPELALN DAIRQLTQLT YTQFQQDTMA LGCLPPTIEP RATDHLEEMR SLIERLLEKG HAYKAENHVL FSVSSIRNDP HYGSFAKRSL DEMRAGARVD VASYKREEMD FVLWKPSAEG EPGWTSPAGI PVLGRPGWHI ECSAMSMAKL LAPYGGGLTC DDPTENVFDI HGGGLDLIFP HHENEIAQSC SAFGTERMAN LWMHNGFFAS
Validated NT Sequence
atggctcacc accaccacca ccatatgaga gagttgcgat tttataatac gcttacacgt aaaaaagaga attttatacc gatagatgca acaagagtcc gtctttatgt ttgtggtccg acggtttatg attatgcgca tataggtaac gcacgtcctg tgattgtttt tgatatttta tttcgcttat tgcgccatgt ttatggcaat gagcatgtta tatatgctcg taatattaca gatgtggatg ataagattaa tgcacgcgca gcttatgaat atccagaact agcactaaac gacgctattc gccaattaac gcagcttaca tatactcaat ttcaacaaga tacaatggca cttggttgtc taccgcccac cattgaaccg cgcgcaactg atcatttaga ggagatgcgt tctttaattg aaagattact tgaaaagggg catgcttata aagcggaaaa tcatgtttta ttttcggtaa gcagtataag aaatgatccc cattatggat catttgcaaa acgctcatta gatgaaatga gagcgggggc acgtgttgat gttgcatctt ataaaagaga agagatggat tttgttttat ggaagccttc tgctgagggg gaaccaggtt ggacatcgcc ggcaggaatt cctgttttgg ggcgtccggg gtggcatatt gaatgttcag caatgtccat ggcaaagtta ttagcaccct atggtggtgg tttaacctgc gatgatccaa cagaaaatgt ttttgatatc catggtggtg gacttgattt gatttttcct catcatgaaa atgagattgc ccaaagttgc tcagcttttg ggactgaacg aatggctaat ctttggatgc ataacggttt ttttgcaagt tgaaggcaag aaaatgtcta aaagtcttag caatttcatc accattcgtt ctattttaga gagtgatttt tttgaattca atggtgtttt aacaaatgaa atgaaacaaa attgggctgg tttatctgcc cgtttttcga tgttacaaac gcattatcgt gaaccgttaa attggaccgc tcagcgttta atgcagtcca gtagcgaatt gtatcgttgg tatgagttgc ttcgttgtga aaagacggag atggagaaga agaatgaggt cattgatgac tctttgataa acgcactcag tgatgacctt aacactccca aagcacttac tcttttacga aaattttata aacgagggaa tgctgtttct cttgcaagtg ggatgaattt ctttggactc ttacggcaag agtgggttag agatggaaaa tgcccattat ttatgagaaa aacctctctt gatgaaaaat ttattgatca gcgcattgcc gaaaggttac gacttatcca taataaagag tggacagctg cggatacaat tcgtgataag cttgcagccg aggggattgt cttaaaagat atcaaggatc cacaaactgg tgaacgtacg
Expected Protein Sequence
MAHHHHHHMR ELRFYNTLTR KKENFIPIDA TRVRLYVCGP TVYDYAHIGN ARPVIVFDIL FRLLRHVYGN EHVIYARNIT DVDDKINARA AYEYPELALN DAIRQLTQLT YTQFQQDTMA LGCLPPTIEP RATDHLEEMR SLIERLLEKG HAYKAENHVL FSVSSIRNDP HYGSFAKRSL DEMRAGARVD VASYKREEMD FVLWKPSAEG EPGWTSPAGI PVLGRPGWHI ECSAMSMAKL LAPYGGGLTC DDPTENVFDI HGGGLDLIFP HHENEIAQSC SAFGTERMAN LWMHNGFLQV EGKKMSKSLS NFITIRSILE SDFFEFNGVL TNEMKQNWAG LSARFSMLQT HYREPLNWTA QRLMQSSSEL YRWYELLRCE KTEMEKKNEV IDDSLINALS DDLNTPKALT LLRKFYKRGN AVSLASGMNF FGLLRQEWVR DGKCPLFMRK TSLDEKFIDQ RIAERLRLIH NKEWTAADTI RDKLAAEGIV LKDIKDPQTG ERTTMWEIKR F
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagag agttgcgatt ttataatacg cttacacgta aaaaagagaa ttttataccg atagatgcaa caagagtccg tctttatgtt tgtggtccga cggtttatga ttatgcgcat ataggtaacg cacgtcctgt gattgttttt gatattttat ttcgcttatt gcgccatgtt tatggcaatg agcatgttat atatgctcgt aatattacag atgtggatga taagattaat gcacgcgcag cttatgaata tccagaacta gcactaaacg acgctattcg ccaattaacg cagcttacat atactcaatt tcaacaagat acaatggcac ttggttgtct accgcccacc attgaaccgc gcgcaactga tcatttagag gagatgcgtt ctttaattga aagattactt gaaaaggggc atgcttataa agcggaaaat catgttttat tttcggtaag cagtataaga aatgatcccc attatggatc atttgcaaaa cgctcattag atgaaatgag agcgggggca cgtgttgatg ttgcatctta taaaagagaa gagatggatt ttgttttatg gaagccttct gctgaggggg aaccaggttg gacatcgccg gcaggaattc ctgttttggg gcgtccgggg tggcatattg aatgttcagc aatgtccatg gcaaagttat tagcacccta tggtggtggt ttaacctgcg atgatccaac agaaaatgtt tttgatatcc atggtggtgg acttgatttg atttttcctc atcatgaaaa tgagattgcc caaagttgct cagcttttgg gactgaacga atggctaatc tttggatgca taacggtttt ttgcaagttg aaggcaagaa aatgtctaaa agtcttagca atttcatcac cattcgttct attttagaga gtgatttttt tgaattcaat ggtgttttaa caaatgaaat gaaacaaaat tgggctggtt tatctgcccg tttttcgatg ttacaaacgc attatcgtga accgttaaat tggaccgctc agcgtttaat gcagtccagt agcgaattgt atcgttggta tgagttgctt cgttgtgaaa agacggagat ggagaagaag aatgaggtca ttgatgactc tttgataaac gcactcagtg atgaccttaa cactcccaaa gcacttactc ttttacgaaa attttataaa cgagggaatg ctgtttctct tgcaagtggg atgaatttct ttggactctt acggcaagag tgggttagag atggaaaatg cccattattt atgagaaaaa cctctcttga tgaaaaattt attgatcagc gcattgccga aaggttacga cttatccata ataaagagtg gacagctgcg gatacaattc gtgataagct tgcagccgag gggattgtct taaaagatat caaggatcca caaactggtg aacgtacgac tatgtgggaa ataaaacggt tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaheA.00133.a.A1.PS00074
PURIFICATION DATe: 10/2/2008
CONCENTRATION: 43.54mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 26
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRELRFYNT LTRKKENFIP IDATRVRLYV CGPTVYDYAH IGNARPVIVF DILFRLLRHV YGNEHVIYAR NITDVDDKIN ARAAYEYPEL ALNDAIRQLT QLTYTQFQQD TMALGCLPPT IEPRATDHLE EMRSLIERLL EKGHAYKAEN HVLFSVSSIR NDPHYGSFAK RSLDEMRAGA RVDVASYKRE EMDFVLWKPS AEGEPGWTSP AGIPVLGRPG WHIECSAMSM AKLLAPYGGG LTCDDPTENV FDIHGGGLDL IFPHHENEIA QSCSAFGTER MANLWMHNGF LQVEGKKMSK SLSNFITIRS ILESDFFEFN GVLTNEMKQN WAGLSARFSM LQTHYREPLN WTAQRLMQSS SELYRWYELL RCEKTEMEKK NEVIDDSLIN ALSDDLNTPK ALTLLRKFYK RGNAVSLASG MNFFGLLRQE WVRDGKCPLF MRKTSLDEKF IDQRIAERLR LIHNKEWTAA DTIRDKLAAE GIVLKDIKDP QTGERTTMWE IKRF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagag agttgcgatt ttataatacg cttacacgta aaaaagagaa ttttataccg atagatgcaa caagagtccg tctttatgtt tgtggtccga cggtttatga ttatgcgcat ataggtaacg cacgtcctgt gattgttttt gatattttat ttcgcttatt gcgccatgtt tatggcaatg agcatgttat atatgctcgt aatattacag atgtggatga taagattaat gcacgcgcag cttatgaata tccagaacta gcactaaacg acgctattcg ccaattaacg cagcttacat atactcaatt tcaacaagat acaatggcac ttggttgtct accgcccacc attgaaccgc gcgcaactga tcatttagag gagatgcgtt ctttaattga aagattactt gaaaaggggc atgcttataa agcggaaaat catgttttat tttcggtaag cagtataaga aatgatcccc attatggatc atttgcaaaa cgctcattag atgaaatgag agcgggggca cgtgttgatg ttgcatctta taaaagagaa gagatggatt ttgttttatg gaagccttct gctgaggggg aaccaggttg gacatcgccg gcaggaattc ctgttttggg gcgtccgggg tggcatattg aatgttcagc aatgtccatg gcaaagttat tagcacccta tggtggtggt ttaacctgcg atgatccaac agaaaatgtt tttgatatcc atggtggtgg acttgatttg atttttcctc atcatgaaaa tgagattgcc caaagttgct cagcttttgg gactgaacga atggctaatc tttggatgca taacggtttt ttgcaagttg aaggcaagaa aatgtctaaa agtcttagca atttcatcac cattcgttct attttagaga gtgatttttt tgaattcaat ggtgttttaa caaatgaaat gaaacaaaat tgggctggtt tatctgcccg tttttcgatg ttacaaacgc attatcgtga accgttaaat tggaccgctc agcgtttaat gcagtccagt agcgaattgt atcgttggta tgagttgctt cgttgtgaaa agacggagat ggagaagaag aatgaggtca ttgatgactc tttgataaac gcactcagtg atgaccttaa cactcccaaa gcacttactc ttttacgaaa attttataaa cgagggaatg ctgtttctct tgcaagtggg atgaatttct ttggactctt acggcaagag tgggttagag atggaaaatg cccattattt atgagaaaaa cctctcttga tgaaaaattt attgatcagc gcattgccga aaggttacga cttatccata ataaagagtg gacagctgcg gatacaattc gtgataagct tgcagccgag gggattgtct taaaagatat caaggatcca caaactggtg aacgtacgac tatgtgggaa ataaaacggt tct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRELRFYNT LTRKKENFIP IDATRVRLYV CGPTVYDYAH IGNARPVIVF DILFRLLRHV YGNEHVIYAR NITDVDDKIN ARAAYEYPEL ALNDAIRQLT QLTYTQFQQD TMALGCLPPT IEPRATDHLE EMRSLIERLL EKGHAYKAEN HVLFSVSSIR NDPHYGSFAK RSLDEMRAGA RVDVASYKRE EMDFVLWKPS AEGEPGWTSP AGIPVLGRPG WHIECSAMSM AKLLAPYGGG LTCDDPTENV FDIHGGGLDL IFPHHENEIA QSCSAFGTER MANLWMHNGF LQVEGKKMSK SLSNFITIRS ILESDFFEFN GVLTNEMKQN WAGLSARFSM LQTHYREPLN WTAQRLMQSS SELYRWYELL RCEKTEMEKK NEVIDDSLIN ALSDDLNTPK ALTLLRKFYK RGNAVSLASG MNFFGLLRQE WVRDGKCPLF MRKTSLDEKF IDQRIAERLR LIHNKEWTAA DTIRDKLAAE GIVLKDIKDP QTGERTTMWE IKRF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGAGAGTTG CGATTTTATA ATACGCTTAC ACGTAAAAAA GAGAATTTTA TACCGATAGA TGCAACAAGA GTCCGTCTTT ATGTTTGTGG TCCGACGGTT TATGATTATG CGCATATAGG TAACGCACGT CCTGTGATTG TTTTTGATAT TTTATTTCGC TTATTGCGCC ATGTTTATGG CAATGAGCAT GTTATATATG CTCGTAATAT TACAGATGTG GATGATAAGA TTAATGCACG CGCAGCTTAT GAATATCCAG AACTAGCACT AAACGACGCT ATTCGCCAAT TAACGCAGCT TACATATACT CAATTTCAAC AAGATACAAT GGCACTTGGT TGTCTACCGC CCACCATTGA ACCGCGCGCA ACTGATCATT TAGAGGAGAT GCGTTCTTTA ATTGAAAGAT TACTTGAAAA GGGGCATGCT TATAAAGCGG AAAATCATGT TTTATTTTCG GTAAGCAGTA TAAGAAATGA TCCCCATTAT GGATCATTTG CAAAACGCTC ATTAGATGAA ATGAGAGCGG GGGCACGTGT TGATGTTGCA TCTTATAAAA GAGAAGAGAT GGATTTTGTT TTATGGAAGC CTTCTGCTGA GGGGGAACCA GGTTGGACAT CGCCGGCAGG AATTCCTGTT TTGGGGCGTC CGGGGTGGCA TATTGAATGT TCAGCAATGT CCATGGCAAA GTTATTAGCA CCCTATGGTG GTGGTTTAAC CTGCGATGAT CCAACAGAAA ATGTTTTTGA TATCCATGGT GGTGGACTTG ATTTGATTTT TCCTCATCAT GAAAATGAGA TTGCCCAAAG TTGCTCAGCT TTTGGGACTG AACGAATGGC TAATCTTTGG ATGCATAACG GTTTTTTGCA AGTTGAAGGC AAGAAAATGT CTAAAAGTCT TAGCAATTTC ATCACCATTC GTTCTATTTT AGAGAGTGAT TTTTTTGAAT TCAATGGTGT TTTAACAAAT GAAATGAAAC AAAATTGGGC TGGTTTATCT GCCCGTTTTT CGATGTTACA AACGCATTAT CGTGAACCGT TAAATTGGAC CGCTCAGCGT TTAATGCAGT CCAGTAGCGA ATTGTATCGT TGGTATGAGT TGCTTCGTTG TGAAAAGACG GAGATGGAGA AGAAGAATGA GGTCATTGAT GACTCTTTGA TAAACGCACT CAGTGATGAC CTTAACACTC CCAAAGCACT TACTCTTTTA CGAAAATTTT ATAAACGAGG GAATGCTGTT TCTCTTGCAA GTGGGATGAA TTTCTTTGGA CTCTTACGGC AAGAGTGGGT TAGAGATGGA AAATGCCCAT TATTTATGAG AAAAACCTCT CTTGATGAAA AATTTATTGA TCAGCGCATT GCCGAAAGGT TACGACTTAT CCATAATAAA GAGTGGACAG CTGCGGATAC AATTCGTGAT AAGCTTGCAG CCGAGGGGAT TGTCTTAAAA GATATCAAGG ATCCACAAAC TGGTGAACGT ACGACTATGT GGGAAATAAA ACGGTTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BaheA.00133.a.B1.PS00096
PURIFICATION DATe: 11/4/2008
CONCENTRATION: 47mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR ELRFYNTLTR KKENFIPIDA TRVRLYVCGP TVYDYAHIGN ARPVIVFDIL FRLLRHVYGN EHVIYARNIT DVDDKINARA AYEYPELALN DAIRQLTQLT YTQFQQDTMA LGCLPPTIEP RATDHLEEMR SLIERLLEKG HAYKAENHVL FSVSSIRNDP HYGSFAKRSL DEMRAGARVD VASYKREEMD FVLWKPSAEG EPGWTSPAGI PVLGRPGWHI ECSAMSMAKL LAPYGGGLTC DDPTENVFDI HGGGLDLIFP HHENEIAQSC SAFGTERMAN LWMHNGFFAS
Validated NT Sequence
atggctcacc accaccacca ccatatgaga gagttgcgat tttataatac gcttacacgt aaaaaagaga attttatacc gatagatgca acaagagtcc gtctttatgt ttgtggtccg acggtttatg attatgcgca tataggtaac gcacgtcctg tgattgtttt tgatatttta tttcgcttat tgcgccatgt ttatggcaat gagcatgtta tatatgctcg taatattaca gatgtggatg ataagattaa tgcacgcgca gcttatgaat atccagaact agcactaaac gacgctattc gccaattaac gcagcttaca tatactcaat ttcaacaaga tacaatggca cttggttgtc taccgcccac cattgaaccg cgcgcaactg atcatttaga ggagatgcgt tctttaattg aaagattact tgaaaagggg catgcttata aagcggaaaa tcatgtttta ttttcggtaa gcagtataag aaatgatccc cattatggat catttgcaaa acgctcatta gatgaaatga gagcgggggc acgtgttgat gttgcatctt ataaaagaga agagatggat tttgttttat ggaagccttc tgctgagggg gaaccaggtt ggacatcgcc ggcaggaatt cctgttttgg ggcgtccggg gtggcatatt gaatgttcag caatgtccat ggcaaagtta ttagcaccct atggtggtgg tttaacctgc gatgatccaa cagaaaatgt ttttgatatc catggtggtg gacttgattt gatttttcct catcatgaaa atgagattgc ccaaagttgc tcagcttttg ggactgaacg aatggctaat ctttggatgc ataacggttt ttttgcaagt tgaaggcaag aaaatgtcta aaagtcttag caatttcatc accattcgtt ctattttaga gagtgatttt tttgaattca atggtgtttt aacaaatgaa atgaaacaaa attgggctgg tttatctgcc cgtttttcga tgttacaaac gcattatcgt gaaccgttaa attggaccgc tcagcgttta atgcagtcca gtagcgaatt gtatcgttgg tatgagttgc ttcgttgtga aaagacggag atggagaaga agaatgaggt cattgatgac tctttgataa acgcactcag tgatgacctt aacactccca aagcacttac tcttttacga aaattttata aacgagggaa tgctgtttct cttgcaagtg ggatgaattt ctttggactc ttacggcaag agtgggttag agatggaaaa tgcccattat ttatgagaaa aacctctctt gatgaaaaat ttattgatca gcgcattgcc gaaaggttac gacttatcca taataaagag tggacagctg cggatacaat tcgtgataag cttgcagccg aggggattgt cttaaaagat atcaaggatc cacaaactgg tgaacgtacg
Expressed Protein Sequence
MAHHHHHHMR ELRFYNTLTR KKENFIPIDA TRVRLYVCGP TVYDYAHIGN ARPVIVFDIL FRLLRHVYGN EHVIYARNIT DVDDKINARA AYEYPELALN DAIRQLTQLT YTQFQQDTMA LGCLPPTIEP RATDHLEEMR SLIERLLEKG HAYKAENHVL FSVSSIRNDP HYGSFAKRSL DEMRAGARVD VASYKREEMD FVLWKPSAEG EPGWTSPAGI PVLGRPGWHI ECSAMSMAKL LAPYGGGLTC DDPTENVFDI HGGGLDLIFP HHENEIAQSC SAFGTERMAN LWMHNGFLQV EGKKMSKSLS NFITIRSILE SDFFEFNGVL TNEMKQNWAG LSARFSMLQT HYREPLNWTA QRLMQSSSEL YRWYELLRCE KTEMEKKNEV IDDSLINALS DDLNTPKALT LLRKFYKRGN AVSLASGMNF FGLLRQEWVR DGKCPLFMRK TSLDEKFIDQ RIAERLRLIH NKEWTAADTI RDKLAAEGIV LKDIKDPQTG ERTTMWEIKR F
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAG AGTTGCGATT TTATAATACG CTTACACGTA AAAAAGAGAA TTTTATACCG ATAGATGCAA CAAGAGTCCG TCTTTATGTT TGTGGTCCGA CGGTTTATGA TTATGCGCAT ATAGGTAACG CACGTCCTGT GATTGTTTTT GATATTTTAT TTCGCTTATT GCGCCATGTT TATGGCAATG AGCATGTTAT ATATGCTCGT AATATTACAG ATGTGGATGA TAAGATTAAT GCACGCGCAG CTTATGAATA TCCAGAACTA GCACTAAACG ACGCTATTCG CCAATTAACG CAGCTTACAT ATACTCAATT TCAACAAGAT ACAATGGCAC TTGGTTGTCT ACCGCCCACC ATTGAACCGC GCGCAACTGA TCATTTAGAG GAGATGCGTT CTTTAATTGA AAGATTACTT GAAAAGGGGC ATGCTTATAA AGCGGAAAAT CATGTTTTAT TTTCGGTAAG CAGTATAAGA AATGATCCCC ATTATGGATC ATTTGCAAAA CGCTCATTAG ATGAAATGAG AGCGGGGGCA CGTGTTGATG TTGCATCTTA TAAAAGAGAA GAGATGGATT TTGTTTTATG GAAGCCTTCT GCTGAGGGGG AACCAGGTTG GACATCGCCG GCAGGAATTC CTGTTTTGGG GCGTCCGGGG TGGCATATTG AATGTTCAGC AATGTCCATG GCAAAGTTAT TAGCACCCTA TGGTGGTGGT TTAACCTGCG ATGATCCAAC AGAAAATGTT TTTGATATCC ATGGTGGTGG ACTTGATTTG ATTTTTCCTC ATCATGAAAA TGAGATTGCC CAAAGTTGCT CAGCTTTTGG GACTGAACGA ATGGCTAATC TTTGGATGCA TAACGGTTTT TTGCAAGTTG AAGGCAAGAA AATGTCTAAA AGTCTTAGCA ATTTCATCAC CATTCGTTCT ATTTTAGAGA GTGATTTTTT TGAATTCAAT GGTGTTTTAA CAAATGAAAT GAAACAAAAT TGGGCTGGTT TATCTGCCCG TTTTTCGATG TTACAAACGC ATTATCGTGA ACCGTTAAAT TGGACCGCTC AGCGTTTAAT GCAGTCCAGT AGCGAATTGT ATCGTTGGTA TGAGTTGCTT CGTTGTGAAA AGACGGAGAT GGAGAAGAAG AATGAGGTCA TTGATGACTC TTTGATAAAC GCACTCAGTG ATGACCTTAA CACTCCCAAA GCACTTACTC TTTTACGAAA ATTTTATAAA CGAGGGAATG CTGTTTCTCT TGCAAGTGGG ATGAATTTCT TTGGACTCTT ACGGCAAGAG TGGGTTAGAG ATGGAAAATG CCCATTATTT ATGAGAAAAA CCTCTCTTGA TGAAAAATTT ATTGATCAGC GCATTGCCGA AAGGTTACGA CTTATCCATA ATAAAGAGTG GACAGCTGCG GATACAATTC GTGATAAGCT TGCAGCCGAG GGGATTGTCT TAAAAGATAT CAAGGATCCA CAAACTGGTG AACGTACGAC TATGTGGGAA ATAAAACGGT TCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT