BaheA.00113.c

3-oxoacyl-(acyl carrier protein) synthase II

CENTER ID: BaheA.00113.c
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00113.c.AE1.GE43401 Full length( BaheA.00113.c ) 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.00113.c.AE1.PS38626 Full length( BaheA.00113.c ) 1 424
BaheA.00113.c.A1.PW27584 Full length( BaheA.00113.c ) 1 424
BaheA.00113.c.A1.PW30565 Full length( BaheA.00113.c ) 1 424

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.797
RefSeq: YP_033556.1
UniProt: A0A0H3LWJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQYNDHFGRP IVAITGAGIV TSLGKGKHEN WQKLTSGVSG IHKITRFPVD ELKTRIAGTV DFLKESTYGA SALSEKLANL AAEEALEQAD LGKTNFNGPL FLAAPPVELE WKARLTLDQE GELNSAPSYV HLLEICSHKL HETLFETTQF GAIAENLQKK FGTKGLPITL STACASGATA IQLGVEAIRR GETNRALTIA TDGSVSAESL IRFSLLSALS TQNDPAEKAA KPFSRDRDGF VMAEGSGALV LESLKSALDR NAIILGILAG CGETADDFHR TRSKPDASPA IEAVRKALED ANITINEIDY INAHGTSTPE NEKMEYLALS TVFGDRLEHI PVSSNKSMIG HTLTAAGAIE AVFSLLTIQS GILPPTINYD DPDPAIPLDV VPNHSRKACV NAVLSNSFGF GGQNMSLVIT AYKG
NT Sequence
ATGCAATATA ACGATCATTT TGGACGTCCA ATTGTAGCAA TAACTGGAGC AGGAATTGTA ACATCACTGG GGAAAGGTAA ACACGAAAAT TGGCAAAAAT TAACAAGTGG TGTTAGCGGT ATCCATAAAA TAACGCGCTT TCCTGTTGAT GAATTAAAAA CACGTATTGC TGGAACAGTC GATTTTCTTA AAGAAAGCAC GTATGGTGCT TCTGCTCTTT CTGAAAAACT TGCTAATCTT GCAGCAGAGG AAGCTTTAGA GCAAGCAGAT CTTGGTAAAA CGAATTTTAA TGGACCACTC TTTTTAGCAG CTCCTCCTGT TGAGCTTGAA TGGAAAGCGC GTCTTACTCT TGATCAAGAA GGAGAATTAA ATAGTGCGCC TTCCTATGTG CATCTTCTTG AAATCTGCAG CCATAAACTC CACGAGACGC TGTTTGAAAC CACACAATTT GGTGCAATTG CAGAAAATCT TCAGAAAAAA TTTGGCACAA AAGGACTTCC TATTACACTT TCCACTGCTT GTGCATCTGG TGCAACAGCC ATTCAATTAG GTGTTGAAGC TATTCGACGG GGAGAAACAA ATCGTGCACT CACAATTGCT ACAGATGGTT CAGTTTCAGC AGAATCCCTT ATACGCTTTT CATTATTATC TGCTCTTTCA ACCCAAAATG ATCCTGCAGA AAAAGCAGCA AAACCCTTTA GCCGTGACCG AGATGGTTTT GTTATGGCAG AAGGATCAGG TGCTCTTGTT CTTGAATCCC TCAAAAGCGC ACTAGATCGT AATGCGATAA TTTTAGGAAT TTTAGCTGGC TGTGGAGAAA CAGCAGATGA TTTTCACCGT ACGCGTTCAA AACCTGATGC ATCTCCAGCA ATTGAAGCCG TTCGCAAAGC TTTAGAGGAT GCGAATATAA CCATCAATGA AATTGATTAT ATTAATGCAC ATGGCACCTC AACACCCGAA AATGAAAAGA TGGAGTATCT CGCATTATCA ACAGTCTTCG GTGATAGACT AGAACATATT CCTGTTTCTT CTAATAAATC AATGATTGGC CACACATTAA CTGCTGCCGG TGCAATAGAG GCTGTTTTTT CGCTTTTAAC GATTCAATCG GGAATACTTC CTCCTACAAT CAATTATGAT GATCCTGATC CTGCTATCCC TTTAGATGTT GTTCCTAACC ATAGCCGTAA GGCCTGTGTC AATGCGGTTT TGTCCAACTC ATTTGGATTC GGCGGTCAAA ATATGAGTCT TGTTATCACA GCCTATAAAG GA
Details for BaheA.00113.c.AE1.GE43401
HARVESTED ON: 9/18/2019
SEQUENCED ON: 10/1/2019
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 29
PERCENT COVERAGE: 36
Validated AA Sequence
MMSILRMANP AKLRARIISX GREYPVDQLI NHVYLPVRQR LVLDHNTSRI MSSMFDGALI EYTAASLFEM RRKPGKEAIL MAWNVEERAR LWLEAWRLSL SGWHISVLAD PIESPRPELF PTQTLIVWTG MAPTRRQNEL LQHWGEQGYK VIFHAP
Validated NT Sequence
tctctcccat gcaatataac gatcattttg gcgcaggttt tgccggtttg ttactcctgt cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca cttgaaattc aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag cgtggccaga gaaaaaataa ttttcagcac attctttcac atgatttcag taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tccacattaa atataatgtt tcatccaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa agtattattg ggtcgtgtac aggcggcggn nntttatgac cgcaaggagg aattgtggct tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc tggcaacgcc gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt gatgaagaag acatacaacg tatcgaagag atcaagcgtt ggataagtaa tggcgtccct gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagc cgcttgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg agaattattt canngggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt gacggcgcat taattgaata cacagcagcc tcgcttttcg aaatgcgccg taagcccggt aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagcc tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgcca cgcccggaac tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga aggcaaaacg aacttttaca acattggggt gagcaaggtt ataaagtcat ctttcatgcc ccctaatttc acctttatcc cgccccttag acagaatgtt ataatctgaa cccacaacag cgtttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag atattccctc tatcacactc tgtacatatc acagcctata aaggagctgg gcaccaccat catcatcatt aacgga
Expected Protein Sequence
MQYNDHFGRP IVAITGAGIV TSLGKGKHEN WQKLTSGVSG IHKITRFPVD ELKTRIAGTV DFLKESTYGA SALSEKLANL AAEEALEQAD LGKTNFNGPL FLAAPPVELE WKARLTLDQE GELNSAPSYV HLLEICSHKL HETLFETTQF GAIAENLQKK FGTKGLPITL STACASGATA IQLGVEAIRR GETNRALTIA TDGSVSAESL IRFSLLSALS TQNDPAEKAA KPFSRDRDGF VMAEGSGALV LESLKSALDR NAIILGILAG CGETADDFHR TRSKPDASPA IEAVRKALED ANITINEIDY INAHGTSTPE NEKMEYLALS TVFGDRLEHI PVSSNKSMIG HTLTAAGAIE AVFSLLTIQS GILPPTINYD DPDPAIPLDV VPNHSRKACV NAVLSNSFGF GGQNMSLVIT AYKGGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gcaatataac gatcattttg gacgtccaat tgtagcaata actggagcag gaattgtaac atcactgggg aaaggtaaac acgaaaattg gcaaaaatta acaagtggtg ttagcggtat ccataaaata acgcgctttc ctgttgatga attaaaaaca cgtattgctg gaacagtcga ttttcttaaa gaaagcacgt atggtgcttc tgctctttct gaaaaacttg ctaatcttgc agcagaggaa gctttagagc aagcagatct tggtaaaacg aattttaatg gaccactctt tttagcagct cctcctgttg agcttgaatg gaaagcgcgt cttactcttg atcaagaagg agaattaaat agtgcgcctt cctatgtgca tcttcttgaa atctgcagcc ataaactcca cgagacgctg tttgaaacca cacaatttgg tgcaattgca gaaaatcttc agaaaaaatt tggcacaaaa ggacttccta ttacactttc cactgcttgt gcatctggtg caacagccat tcaattaggt gttgaagcta ttcgacgggg agaaacaaat cgtgcactca caattgctac agatggttca gtttcagcag aatcccttat acgcttttca ttattatctg ctctttcaac ccaaaatgat cctgcagaaa aagcagcaaa accctttagc cgtgaccgag atggttttgt tatggcagaa ggatcaggtg ctcttgttct tgaatccctc aaaagcgcac tagatcgtaa tgcgataatt ttaggaattt tagctggctg tggagaaaca gcagatgatt ttcaccgtac gcgttcaaaa cctgatgcat ctccagcaat tgaagccgtt cgcaaagctt tagaggatgc gaatataacc atcaatgaaa ttgattatat taatgcacat ggcacctcaa cacccgaaaa tgaaaagatg gagtatctcg cattatcaac agtcttcggt gatagactag aacatattcc tgtttcttct aataaatcaa tgattggcca cacattaact gctgccggtg caatagaggc tgttttttcg cttttaacga ttcaatcggg aatacttcct cctacaatca attatgatga tcctgatcct gctatccctt tagatgttgt tcctaaccat agccgtaagg cctgtgtcaa tgcggttttg tccaactcat ttggattcgg cggtcaaaat atgagtcttg ttatcacagc ctataaagga gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for BaheA.00113.c.AE1.PS38626
PURIFICATION DATe: 12/31/2019
CONCENTRATION: 24.18mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 29
PERCENT COVERAGE: 36
Protocol Notes
notes unavailable
Validated AA Sequence
MMSILRMANP AKLRARIISX GREYPVDQLI NHVYLPVRQR LVLDHNTSRI MSSMFDGALI EYTAASLFEM RRKPGKEAIL MAWNVEERAR LWLEAWRLSL SGWHISVLAD PIESPRPELF PTQTLIVWTG MAPTRRQNEL LQHWGEQGYK VIFHAP
Validated NT Sequence
tctctcccat gcaatataac gatcattttg gcgcaggttt tgccggtttg ttactcctgt cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca cttgaaattc aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag cgtggccaga gaaaaaataa ttttcagcac attctttcac atgatttcag taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tccacattaa atataatgtt tcatccaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa agtattattg ggtcgtgtac aggcggcggn nntttatgac cgcaaggagg aattgtggct tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc tggcaacgcc gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt gatgaagaag acatacaacg tatcgaagag atcaagcgtt ggataagtaa tggcgtccct gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagc cgcttgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg agaattattt canngggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt gacggcgcat taattgaata cacagcagcc tcgcttttcg aaatgcgccg taagcccggt aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagcc tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgcca cgcccggaac tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga aggcaaaacg aacttttaca acattggggt gagcaaggtt ataaagtcat ctttcatgcc ccctaatttc acctttatcc cgccccttag acagaatgtt ataatctgaa cccacaacag cgtttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag atattccctc tatcacactc tgtacatatc acagcctata aaggagctgg gcaccaccat catcatcatt aacgga
Expressed Protein Sequence
MQYNDHFGRP IVAITGAGIV TSLGKGKHEN WQKLTSGVSG IHKITRFPVD ELKTRIAGTV DFLKESTYGA SALSEKLANL AAEEALEQAD LGKTNFNGPL FLAAPPVELE WKARLTLDQE GELNSAPSYV HLLEICSHKL HETLFETTQF GAIAENLQKK FGTKGLPITL STACASGATA IQLGVEAIRR GETNRALTIA TDGSVSAESL IRFSLLSALS TQNDPAEKAA KPFSRDRDGF VMAEGSGALV LESLKSALDR NAIILGILAG CGETADDFHR TRSKPDASPA IEAVRKALED ANITINEIDY INAHGTSTPE NEKMEYLALS TVFGDRLEHI PVSSNKSMIG HTLTAAGAIE AVFSLLTIQS GILPPTINYD DPDPAIPLDV VPNHSRKACV NAVLSNSFGF GGQNMSLVIT AYKGGHHHHH H
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GCAATATAAC GATCATTTTG GACGTCCAAT TGTAGCAATA ACTGGAGCAG GAATTGTAAC ATCACTGGGG AAAGGTAAAC ACGAAAATTG GCAAAAATTA ACAAGTGGTG TTAGCGGTAT CCATAAAATA ACGCGCTTTC CTGTTGATGA ATTAAAAACA CGTATTGCTG GAACAGTCGA TTTTCTTAAA GAAAGCACGT ATGGTGCTTC TGCTCTTTCT GAAAAACTTG CTAATCTTGC AGCAGAGGAA GCTTTAGAGC AAGCAGATCT TGGTAAAACG AATTTTAATG GACCACTCTT TTTAGCAGCT CCTCCTGTTG AGCTTGAATG GAAAGCGCGT CTTACTCTTG ATCAAGAAGG AGAATTAAAT AGTGCGCCTT CCTATGTGCA TCTTCTTGAA ATCTGCAGCC ATAAACTCCA CGAGACGCTG TTTGAAACCA CACAATTTGG TGCAATTGCA GAAAATCTTC AGAAAAAATT TGGCACAAAA GGACTTCCTA TTACACTTTC CACTGCTTGT GCATCTGGTG CAACAGCCAT TCAATTAGGT GTTGAAGCTA TTCGACGGGG AGAAACAAAT CGTGCACTCA CAATTGCTAC AGATGGTTCA GTTTCAGCAG AATCCCTTAT ACGCTTTTCA TTATTATCTG CTCTTTCAAC CCAAAATGAT CCTGCAGAAA AAGCAGCAAA ACCCTTTAGC CGTGACCGAG ATGGTTTTGT TATGGCAGAA GGATCAGGTG CTCTTGTTCT TGAATCCCTC AAAAGCGCAC TAGATCGTAA TGCGATAATT TTAGGAATTT TAGCTGGCTG TGGAGAAACA GCAGATGATT TTCACCGTAC GCGTTCAAAA CCTGATGCAT CTCCAGCAAT TGAAGCCGTT CGCAAAGCTT TAGAGGATGC GAATATAACC ATCAATGAAA TTGATTATAT TAATGCACAT GGCACCTCAA CACCCGAAAA TGAAAAGATG GAGTATCTCG CATTATCAAC AGTCTTCGGT GATAGACTAG AACATATTCC TGTTTCTTCT AATAAATCAA TGATTGGCCA CACATTAACT GCTGCCGGTG CAATAGAGGC TGTTTTTTCG CTTTTAACGA TTCAATCGGG AATACTTCCT CCTACAATCA ATTATGATGA TCCTGATCCT GCTATCCCTT TAGATGTTGT TCCTAACCAT AGCCGTAAGG CCTGTGTCAA TGCGGTTTTG TCCAACTCAT TTGGATTCGG CGGTCAAAAT ATGAGTCTTG TTATCACAGC CTATAAAGGA GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for BaheA.00113.c.A1.PW27584
PURIFICATION DATe: 9/22/2009
CONCENTRATION: 25.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 23
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MQYNDHFGRP IVAITGAGIV TSLGKGKHEN WQKLTSGVSG IHKITRFPVD ELKTRIAGTV DFLKESTYGA SALSEKLANL AAEEALEQAD LGKTNFNGPL FLAAPPVELE WKARLTLDQE GELNSAPSYV HLLEICSHKL HETLFETTQF GAIAENLQKK FGTKGLPITL STACASGATA IQLGVEAIRR GETNRALTIA TDGSVSAESL IRFSLLSALS TQNDPAEKAA KPFSRDRDGF VMAEGSGALV LESLKSALDR NAIILGILAG CGETADDFHR TRSKPDASPA IEAVRKALED ANITINEIDY INAHGTSTPE NEKMEYLALS TVFGDRLEHI PVSSNKSMIG HTLTAAGAIE AVFSLLTIQS GILPPTINYD DPDPAIPLDV VPNHSRKACV NAVLSNSFGF GGQNMSLVIT AY
Validated NT Sequence
tataggctgt gataacaaga ctcatatttt gaccgccgaa tccaaatgag ttggacaaaa ccgcattgac acaggcctta cggctatggt taggaacaac atctaaaggg atagcaggat caggatcatc ataattgatt gtaggaggaa gtattcccga ttgaatcgtt aaaagcgaaa aaacagcctc tattgcaccg gcagcagtta atgtgtggcc aatcattgat ttattagaag aaacaggaat atgttctagt ctatcaccga agactgttga taatgcgaga tactccatct tttcattttc gggtgttgag gtgccatgtg cattaatata atcaatttca ttgatggtta tattcgcatc ctctaaagct ttgcgaacgg cttcaattgc tggagatgca tcaggttttg aacgcgtacg gtgaaaatca tctgctgttt ctccacagcc agctaaaatt cctaaaatta tcgcattacg atctagtgcg cttttgaggg attcaagaac aagagcacct gatccttctg ccataacaaa accatctcgg tcacggctaa agggttttgc tgctttttct gcaggatcat tttgggttga aagagcagat aataatgaaa agcgtataag ggattctgct gaaactgaac catctgtagc aattgtgagt gcacgatttg tttctccccg tcgaatagct tcaacaccta attgaatggc tgttgcacca gatgcacaag cagtggaaag tgtaatagga agtccttttg tgccaaattt tttctgaaga ttttctgcaa ttgcaccaaa ttgtgtggtt tcaaacagcg tctcgtggag tttatggctg cagatttcaa gaagatgcac ataggaaggc gcactattta attctccttc ttgatcaaga gtaagacgcg ctttccattc aagctcaaca ggaggagctg ctaaaaagag tggtccatta aaattcgttt taccaagatc tgcttgctct aaagcttcct ctgctgcaag attagcaagt ttttcagaaa gagcagaagc accatacgtg ctttctttaa gaaaatcgac tgttccagca atacgtgttt ttaattcatc aacaggaaag cgcgttattt tatggatacc gctaacacca cttgttaatt tttgccaatt ttcgtgttta cctttcccca gtgatgttac aattcctgct ccagttattg ctacaattgg acgtccaaaa tgatcgttat attgcatcga accaggaccc tgggtctgag cttccagggt accc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNDHFGR PIVAITGAGI VTSLGKGKHE NWQKLTSGVS GIHKITRFPV DELKTRIAGT VDFLKESTYG ASALSEKLAN LAAEEALEQA DLGKTNFNGP LFLAAPPVEL EWKARLTLDQ EGELNSAPSY VHLLEICSHK LHETLFETTQ FGAIAENLQK KFGTKGLPIT LSTACASGAT AIQLGVEAIR RGETNRALTI ATDGSVSAES LIRFSLLSAL STQNDPAEKA AKPFSRDRDG FVMAEGSGAL VLESLKSALD RNAIILGILA GCGETADDFH RTRSKPDASP AIEAVRKALE DANITINEID YINAHGTSTP ENEKMEYLAL STVFGDRLEH IPVSSNKSMI GHTLTAAGAI EAVFSLLTIQ SGILPPTINY DDPDPAIPLD VVPNHSRKAC VNAVLSNSFG FGGQNMSLVI TAYKG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATATAAC GATCATTTTG GACGTCCAAT TGTAGCAATA ACTGGAGCAG GAATTGTAAC ATCACTGGGG AAAGGTAAAC ACGAAAATTG GCAAAAATTA ACAAGTGGTG TTAGCGGTAT CCATAAAATA ACGCGCTTTC CTGTTGATGA ATTAAAAACA CGTATTGCTG GAACAGTCGA TTTTCTTAAA GAAAGCACGT ATGGTGCTTC TGCTCTTTCT GAAAAACTTG CTAATCTTGC AGCAGAGGAA GCTTTAGAGC AAGCAGATCT TGGTAAAACG AATTTTAATG GACCACTCTT TTTAGCAGCT CCTCCTGTTG AGCTTGAATG GAAAGCGCGT CTTACTCTTG ATCAAGAAGG AGAATTAAAT AGTGCGCCTT CCTATGTGCA TCTTCTTGAA ATCTGCAGCC ATAAACTCCA CGAGACGCTG TTTGAAACCA CACAATTTGG TGCAATTGCA GAAAATCTTC AGAAAAAATT TGGCACAAAA GGACTTCCTA TTACACTTTC CACTGCTTGT GCATCTGGTG CAACAGCCAT TCAATTAGGT GTTGAAGCTA TTCGACGGGG AGAAACAAAT CGTGCACTCA CAATTGCTAC AGATGGTTCA GTTTCAGCAG AATCCCTTAT ACGCTTTTCA TTATTATCTG CTCTTTCAAC CCAAAATGAT CCTGCAGAAA AAGCAGCAAA ACCCTTTAGC CGTGACCGAG ATGGTTTTGT TATGGCAGAA GGATCAGGTG CTCTTGTTCT TGAATCCCTC AAAAGCGCAC TAGATCGTAA TGCGATAATT TTAGGAATTT TAGCTGGCTG TGGAGAAACA GCAGATGATT TTCACCGTAC GCGTTCAAAA CCTGATGCAT CTCCAGCAAT TGAAGCCGTT CGCAAAGCTT TAGAGGATGC GAATATAACC ATCAATGAAA TTGATTATAT TAATGCACAT GGCACCTCAA CACCCGAAAA TGAAAAGATG GAGTATCTCG CATTATCAAC AGTCTTCGGT GATAGACTAG AACATATTCC TGTTTCTTCT AATAAATCAA TGATTGGCCA CACATTAACT GCTGCCGGTG CAATAGAGGC TGTTTTTTCG CTTTTAACGA TTCAATCGGG AATACTTCCT CCTACAATCA ATTATGATGA TCCTGATCCT GCTATCCCTT TAGATGTTGT TCCTAACCAT AGCCGTAAGG CCTGTGTCAA TGCGGTTTTG TCCAACTCAT TTGGATTCGG CGGTCAAAAT ATGAGTCTTG TTATCACAGC CTATAAAGGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BaheA.00113.c.A1.PW30565
PURIFICATION DATe: 9/22/2010
CONCENTRATION: 15.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MQYNDHFGRP IVAITGAGIV TSLGKGKHEN WQKLTSGVSG IHKITRFPVD ELKTRIAGTV DFLKESTYGA SALSEKLANL AAEEALEQAD LGKTNFNGPL FLAAPPVELE WKARLTLDQE GELNSAPSYV HLLEICSHKL HETLFETTQF GAIAENLQKK FGTKGLPITL STACASGATA IQLGVEAIRR GETNRALTIA TDGSVSAESL IRFSLLSALS TQNDPAEKAA KPFSRDRDGF VMAEGSGALV LESLKSALDR NAIILGILAG CGETADDFHR TRSKPDASPA IEAVRKALED ANITINEIDY INAHGTSTPE NEKMEYLALS TVFGDRLEHI PVSSNKSMIG HTLTAAGAIE AVFSLLTIQS GILPPTINYD DPDPAIPLDV VPNHSRKACV NAVLSNSFGF GGQNMSLVIT AY
Validated NT Sequence
tataggctgt gataacaaga ctcatatttt gaccgccgaa tccaaatgag ttggacaaaa ccgcattgac acaggcctta cggctatggt taggaacaac atctaaaggg atagcaggat caggatcatc ataattgatt gtaggaggaa gtattcccga ttgaatcgtt aaaagcgaaa aaacagcctc tattgcaccg gcagcagtta atgtgtggcc aatcattgat ttattagaag aaacaggaat atgttctagt ctatcaccga agactgttga taatgcgaga tactccatct tttcattttc gggtgttgag gtgccatgtg cattaatata atcaatttca ttgatggtta tattcgcatc ctctaaagct ttgcgaacgg cttcaattgc tggagatgca tcaggttttg aacgcgtacg gtgaaaatca tctgctgttt ctccacagcc agctaaaatt cctaaaatta tcgcattacg atctagtgcg cttttgaggg attcaagaac aagagcacct gatccttctg ccataacaaa accatctcgg tcacggctaa agggttttgc tgctttttct gcaggatcat tttgggttga aagagcagat aataatgaaa agcgtataag ggattctgct gaaactgaac catctgtagc aattgtgagt gcacgatttg tttctccccg tcgaatagct tcaacaccta attgaatggc tgttgcacca gatgcacaag cagtggaaag tgtaatagga agtccttttg tgccaaattt tttctgaaga ttttctgcaa ttgcaccaaa ttgtgtggtt tcaaacagcg tctcgtggag tttatggctg cagatttcaa gaagatgcac ataggaaggc gcactattta attctccttc ttgatcaaga gtaagacgcg ctttccattc aagctcaaca ggaggagctg ctaaaaagag tggtccatta aaattcgttt taccaagatc tgcttgctct aaagcttcct ctgctgcaag attagcaagt ttttcagaaa gagcagaagc accatacgtg ctttctttaa gaaaatcgac tgttccagca atacgtgttt ttaattcatc aacaggaaag cgcgttattt tatggatacc gctaacacca cttgttaatt tttgccaatt ttcgtgttta cctttcccca gtgatgttac aattcctgct ccagttattg ctacaattgg acgtccaaaa tgatcgttat attgcatcga accaggaccc tgggtctgag cttccagggt accc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNDHFGR PIVAITGAGI VTSLGKGKHE NWQKLTSGVS GIHKITRFPV DELKTRIAGT VDFLKESTYG ASALSEKLAN LAAEEALEQA DLGKTNFNGP LFLAAPPVEL EWKARLTLDQ EGELNSAPSY VHLLEICSHK LHETLFETTQ FGAIAENLQK KFGTKGLPIT LSTACASGAT AIQLGVEAIR RGETNRALTI ATDGSVSAES LIRFSLLSAL STQNDPAEKA AKPFSRDRDG FVMAEGSGAL VLESLKSALD RNAIILGILA GCGETADDFH RTRSKPDASP AIEAVRKALE DANITINEID YINAHGTSTP ENEKMEYLAL STVFGDRLEH IPVSSNKSMI GHTLTAAGAI EAVFSLLTIQ SGILPPTINY DDPDPAIPLD VVPNHSRKAC VNAVLSNSFG FGGQNMSLVI TAYKG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATATAAC GATCATTTTG GACGTCCAAT TGTAGCAATA ACTGGAGCAG GAATTGTAAC ATCACTGGGG AAAGGTAAAC ACGAAAATTG GCAAAAATTA ACAAGTGGTG TTAGCGGTAT CCATAAAATA ACGCGCTTTC CTGTTGATGA ATTAAAAACA CGTATTGCTG GAACAGTCGA TTTTCTTAAA GAAAGCACGT ATGGTGCTTC TGCTCTTTCT GAAAAACTTG CTAATCTTGC AGCAGAGGAA GCTTTAGAGC AAGCAGATCT TGGTAAAACG AATTTTAATG GACCACTCTT TTTAGCAGCT CCTCCTGTTG AGCTTGAATG GAAAGCGCGT CTTACTCTTG ATCAAGAAGG AGAATTAAAT AGTGCGCCTT CCTATGTGCA TCTTCTTGAA ATCTGCAGCC ATAAACTCCA CGAGACGCTG TTTGAAACCA CACAATTTGG TGCAATTGCA GAAAATCTTC AGAAAAAATT TGGCACAAAA GGACTTCCTA TTACACTTTC CACTGCTTGT GCATCTGGTG CAACAGCCAT TCAATTAGGT GTTGAAGCTA TTCGACGGGG AGAAACAAAT CGTGCACTCA CAATTGCTAC AGATGGTTCA GTTTCAGCAG AATCCCTTAT ACGCTTTTCA TTATTATCTG CTCTTTCAAC CCAAAATGAT CCTGCAGAAA AAGCAGCAAA ACCCTTTAGC CGTGACCGAG ATGGTTTTGT TATGGCAGAA GGATCAGGTG CTCTTGTTCT TGAATCCCTC AAAAGCGCAC TAGATCGTAA TGCGATAATT TTAGGAATTT TAGCTGGCTG TGGAGAAACA GCAGATGATT TTCACCGTAC GCGTTCAAAA CCTGATGCAT CTCCAGCAAT TGAAGCCGTT CGCAAAGCTT TAGAGGATGC GAATATAACC ATCAATGAAA TTGATTATAT TAATGCACAT GGCACCTCAA CACCCGAAAA TGAAAAGATG GAGTATCTCG CATTATCAAC AGTCTTCGGT GATAGACTAG AACATATTCC TGTTTCTTCT AATAAATCAA TGATTGGCCA CACATTAACT GCTGCCGGTG CAATAGAGGC TGTTTTTTCG CTTTTAACGA TTCAATCGGG AATACTTCCT CCTACAATCA ATTATGATGA TCCTGATCCT GCTATCCCTT TAGATGTTGT TCCTAACCAT AGCCGTAAGG CCTGTGTCAA TGCGGTTTTG TCCAACTCAT TTGGATTCGG CGGTCAAAAT ATGAGTCTTG TTATCACAGC CTATAAAGGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA