BaheA.00113.b
3-oxoacyl-(acyl carrier protein) synthase II
| CENTER ID: | BaheA.00113.b |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00113.b.A1.GE25178 | Full length( BaheA.00113.b ) | 1 | 420 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00113.b.A1.PW25216 | Full length( BaheA.00113.b ) | 1 | 420 |
Structures
3E60
DEPOSITED: 8/14/2008
DETERMINATION: XRay
CLONE: BaheA.00113.b.A1.GE25178
PROTEIN: BaheA.00113.b.A1.PW25216
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126985 |
| PATRIC ID: | fig|283166.5.peg.586 |
| RefSeq: | YP_033372.1 |
| UniProt: | A0A0H3LW83 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRVVITGLG LVSPLAGDVE YSWKRLLEGK SGVRRITEFD VSDLSCQIAA RIPVGDGTNG TYNADLHMES KEQRKVDAFI VYAIAAADQA LADAEWFPKS DEDQICTGVL IGSGIGGIEG IVEAGYTLRD KGPRRISPFF IPGRLINLAS GYVSIKYGLR GPNHSVVTAC STGAHAIGDA ARLIALGDAD VMLAGGTESP INRISLAGFS ACRALSTCRN DDPERASRPY DVDRDGFVMG EGAAIVVLEE LEHAKKRGAR IYAEIIGYGL SGDAYHITAP SESGEGAQRS MMAALKRAQV NVSELDYINA HGTSTMADVI ELAAVERVLG YYAPQVSMSS TKSSIGHLLG AAGAAEAIFC VLAIRDNIAP ATLNLENPSI ETKIDLVPHK PRERKIDTVL SNSFGFGGTN ASLVMRRFSE
NT Sequence
ATGAGGCGTG TTGTTATTAC TGGTTTAGGA TTGGTATCTC CATTAGCTGG TGATGTCGAG TATAGTTGGA AACGACTTCT TGAAGGAAAA AGTGGTGTTC GACGCATTAC TGAATTTGAT GTATCCGACT TATCGTGCCA GATTGCTGCG CGTATCCCCG TAGGGGATGG TACAAATGGT ACATATAATG CTGATTTGCA TATGGAATCC AAGGAGCAGC GTAAGGTTGA TGCATTTATT GTTTATGCAA TTGCAGCCGC TGACCAAGCA CTTGCAGATG CTGAGTGGTT TCCCAAGAGT GATGAAGACC AGATTTGCAC AGGTGTGTTA ATTGGTTCGG GTATTGGTGG CATTGAAGGG ATTGTTGAGG CTGGTTATAC GCTTCGTGAT AAAGGTCCAC GGCGTATTTC TCCTTTTTTT ATTCCAGGAC GTTTGATTAA TCTTGCTTCT GGTTATGTAT CTATTAAATA TGGTTTGCGT GGTCCTAATC ATTCGGTTGT AACGGCCTGT TCAACAGGTG CACATGCCAT TGGTGATGCA GCACGTTTGA TTGCGTTGGG TGATGCAGAT GTGATGCTAG CAGGTGGCAC TGAATCCCCG ATAAATCGAA TATCTCTTGC TGGTTTTTCT GCTTGTCGAG CTCTTTCTAC TTGTCGTAAT GATGATCCTG AACGGGCATC ACGTCCTTAT GATGTTGATC GTGATGGTTT TGTGATGGGG GAAGGAGCAG CGATTGTTGT TTTAGAAGAG CTTGAACATG CAAAAAAACG AGGTGCTCGT ATTTATGCTG AGATTATTGG CTATGGTCTA TCTGGTGACG CTTACCACAT TACAGCACCT TCAGAGAGTG GTGAAGGTGC GCAGCGTAGT ATGATGGCTG CTCTTAAGCG TGCACAAGTG AATGTAAGCG AACTTGATTA CATTAATGCT CACGGTACAT CGACGATGGC TGATGTTATA GAATTGGCTG CTGTTGAGCG TGTTTTGGGC TATTATGCAC CACAAGTATC CATGTCATCA ACAAAGTCAT CTATAGGACA TTTACTTGGT GCAGCTGGTG CAGCTGAGGC TATTTTTTGT GTTTTAGCTA TACGAGATAA TATAGCTCCA GCAACACTTA ATCTTGAAAA TCCATCAATT GAAACGAAAA TTGACCTTGT TCCACATAAA CCGCGCGAGC GAAAAATTGA TACAGTTCTA TCCAATTCTT TTGGATTTGG TGGAACAAAT GCGTCTTTAG TGATGCGGCG TTTTTCGGAA
Details for BaheA.00113.b.A1.GE25178
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVITGL GLVSPLAGDV EYSWKRLLEG KSGVRRITEF DVSDLSCQIA ARIPVGDGTN GTYNADLHME SKEQRKVDAF IVYAIAAADQ ALADAEWFPK SDEDQICTGV LIGSGIGGIE GIVEAGYTLR DKGPRRISPF FIPGRLINLA SGYVSIKYGL RGPNHSVVTA CSTGAHAIGD AARLIALGDA DVMLAGGTES PINRISLAGF SACRALSTCR NDDPERASRP YDVDRDGFVM GEGAAIVVLE ELEHAKKRGA RIYAEIIGYG LSGDAYHITA PSESGEGAQR SMMAALKRAQ VNVSELDYIN AHGTSTMADV IELAAVERVL GYYAPQVSMS STKSSIGHLL GAAGAAEAIF CVLAIRDNIA PATLNLENPS IETKIDLVPH KPRERKIDTV LSNSFGFGGT NASLVMRRFS E
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaggc gtgttgttat tactggttta ggattggtat ctccattagc tggtgatgtc gagtatagtt ggaaacgact tcttgaagga aaaagtggtg ttcgacgcat tactgaattt gatgtatccg acttatcgtg ccagattgct gcgcgtatcc ccgtagggga tggtacaaat ggtacatata atgctgattt gcatatggaa tccaaggagc agcgtaaggt tgatgcattt attgtttatg caattgcagc cgctgaccaa gcacttgcag atgctgagtg gtttcccaag agtgatgaag accagatttg cacaggtgtg ttaattggtt cgggtattgg tggcattgaa gggattgttg aggctggtta tacgcttcgt gataaaggtc cacggcgtat ttctcctttt tttattccag gacgtttgat taatcttgct tctggttatg tatctattaa atatggtttg cgtggtccta atcattcggt tgtaacggcc tgttcaacag gtgcacatgc cattggtgat gcagcacgtt tgattgcgtt gggtgatgca gatgtgatgc tagcaggtgg cactgaatcc ccgataaatc gaatatctct tgctggtttt tctgcttgtc gagctctttc tacttgtcgt aatgatgatc ctgaacgggc atcacgtcct tatgatgttg atcgtgatgg ttttgtgatg ggggaaggag cagcgattgt tgttttagaa gagcttgaac atgcaaaaaa acgaggtgct cgtatttatg ctgagattat tggctatggt ctatctggtg acgcttacca cattacagca ccttcagaga gtggtgaagg tgcgcagcgt agtatgatgg ctgctcttaa gcgtgcacaa gtgaatgtaa gcgaacttga ttacattaat gctcacggta catcgacgat ggctgatgtt atagaattgg ctgctgttga gcgtgttttg ggctattatg caccacaagt atccatgtca tcaacaaagt catctatagg acatttactt ggtgcagctg gtgcagctga ggctattttt tgtgttttag ctatacgaga taatatagct ccagcaacac ttaatcttga aaatccatca attgaaacga aaattgacct tgttccacat aaaccgcgcg agcgaaaaat tgatacagtt ctatccaatt cttttggatt tggtggaaca aatgcgtctt tagtgatgcg gcgtttttcg gaataata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVITGL GLVSPLAGDV EYSWKRLLEG KSGVRRITEF DVSDLSCQIA ARIPVGDGTN GTYNADLHME SKEQRKVDAF IVYAIAAADQ ALADAEWFPK SDEDQICTGV LIGSGIGGIE GIVEAGYTLR DKGPRRISPF FIPGRLINLA SGYVSIKYGL RGPNHSVVTA CSTGAHAIGD AARLIALGDA DVMLAGGTES PINRISLAGF SACRALSTCR NDDPERASRP YDVDRDGFVM GEGAAIVVLE ELEHAKKRGA RIYAEIIGYG LSGDAYHITA PSESGEGAQR SMMAALKRAQ VNVSELDYIN AHGTSTMADV IELAAVERVL GYYAPQVSMS STKSSIGHLL GAAGAAEAIF CVLAIRDNIA PATLNLENPS IETKIDLVPH KPRERKIDTV LSNSFGFGGT NASLVMRRFS E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaggcgtgtt gttattactg gtttaggatt ggtatctcca ttagctggtg atgtcgagta tagttggaaa cgacttcttg aaggaaaaag tggtgttcga cgcattactg aatttgatgt atccgactta tcgtgccaga ttgctgcgcg tatccccgta ggggatggta caaatggtac atataatgct gatttgcata tggaatccaa ggagcagcgt aaggttgatg catttattgt ttatgcaatt gcagccgctg accaagcact tgcagatgct gagtggtttc ccaagagtga tgaagaccag atttgcacag gtgtgttaat tggttcgggt attggtggca ttgaagggat tgttgaggct ggttatacgc ttcgtgataa aggtccacgg cgtatttctc ctttttttat tccaggacgt ttgattaatc ttgcttctgg ttatgtatct attaaatatg gtttgcgtgg tcctaatcat tcggttgtaa cggcctgttc aacaggtgca catgccattg gtgatgcagc acgtttgatt gcgttgggtg atgcagatgt gatgctagca ggtggcactg aatccccgat aaatcgaata tctcttgctg gtttttctgc ttgtcgagct ctttctactt gtcgtaatga tgatcctgaa cgggcatcac gtccttatga tgttgatcgt gatggttttg tgatggggga aggagcagcg attgttgttt tagaagagct tgaacatgca aaaaaacgag gtgctcgtat ttatgctgag attattggct atggtctatc tggtgacgct taccacatta cagcaccttc agagagtggt gaaggtgcgc agcgtagtat gatggctgct cttaagcgtg cacaagtgaa tgtaagcgaa cttgattaca ttaatgctca cggtacatcg acgatggctg atgttataga attggctgct gttgagcgtg ttttgggcta ttatgcacca caagtatcca tgtcatcaac aaagtcatct ataggacatt tacttggtgc agctggtgca gctgaggcta ttttttgtgt tttagctata cgagataata tagctccagc aacacttaat cttgaaaatc catcaattga aacgaaaatt gaccttgttc cacataaacc gcgcgagcga aaaattgata cagttctatc caattctttt ggatttggtg gaacaaatgc gtctttagtg atgcggcgtt tttcggaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BaheA.00113.b.A1.PW25216
| PURIFICATION DATe: | 6/16/2008 |
| CONCENTRATION: | 6.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 66 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVITGL GLVSPLAGDV EYSWKRLLEG KSGVRRITEF DVSDLSCQIA ARIPVGDGTN GTYNADLHME SKEQRKVDAF IVYAIAAADQ ALADAEWFPK SDEDQICTGV LIGSGIGGIE GIVEAGYTLR DKGPRRISPF FIPGRLINLA SGYVSIKYGL RGPNHSVVTA CSTGAHAIGD AARLIALGDA DVMLAGGTES PINRISLAGF SACRALSTCR NDDPERASRP YDVDRDGFVM GEGAAIVVLE ELEHAKKRGA RIYAEIIGYG LSGDAYHITA PSESGEGAQR SMMAALKRAQ VNVSELDYIN AHGTSTMADV IELAAVERVL GYYAPQVSMS STKSSIGHLL GAAGAAEAIF CVLAIRDNIA PATLNLENPS IETKIDLVPH KPRERKIDTV LSNSFGFGGT NASLVMRRFS E
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaggc gtgttgttat tactggttta ggattggtat ctccattagc tggtgatgtc gagtatagtt ggaaacgact tcttgaagga aaaagtggtg ttcgacgcat tactgaattt gatgtatccg acttatcgtg ccagattgct gcgcgtatcc ccgtagggga tggtacaaat ggtacatata atgctgattt gcatatggaa tccaaggagc agcgtaaggt tgatgcattt attgtttatg caattgcagc cgctgaccaa gcacttgcag atgctgagtg gtttcccaag agtgatgaag accagatttg cacaggtgtg ttaattggtt cgggtattgg tggcattgaa gggattgttg aggctggtta tacgcttcgt gataaaggtc cacggcgtat ttctcctttt tttattccag gacgtttgat taatcttgct tctggttatg tatctattaa atatggtttg cgtggtccta atcattcggt tgtaacggcc tgttcaacag gtgcacatgc cattggtgat gcagcacgtt tgattgcgtt gggtgatgca gatgtgatgc tagcaggtgg cactgaatcc ccgataaatc gaatatctct tgctggtttt tctgcttgtc gagctctttc tacttgtcgt aatgatgatc ctgaacgggc atcacgtcct tatgatgttg atcgtgatgg ttttgtgatg ggggaaggag cagcgattgt tgttttagaa gagcttgaac atgcaaaaaa acgaggtgct cgtatttatg ctgagattat tggctatggt ctatctggtg acgcttacca cattacagca ccttcagaga gtggtgaagg tgcgcagcgt agtatgatgg ctgctcttaa gcgtgcacaa gtgaatgtaa gcgaacttga ttacattaat gctcacggta catcgacgat ggctgatgtt atagaattgg ctgctgttga gcgtgttttg ggctattatg caccacaagt atccatgtca tcaacaaagt catctatagg acatttactt ggtgcagctg gtgcagctga ggctattttt tgtgttttag ctatacgaga taatatagct ccagcaacac ttaatcttga aaatccatca attgaaacga aaattgacct tgttccacat aaaccgcgcg agcgaaaaat tgatacagtt ctatccaatt cttttggatt tggtggaaca aatgcgtctt tagtgatgcg gcgtttttcg gaataata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVITGL GLVSPLAGDV EYSWKRLLEG KSGVRRITEF DVSDLSCQIA ARIPVGDGTN GTYNADLHME SKEQRKVDAF IVYAIAAADQ ALADAEWFPK SDEDQICTGV LIGSGIGGIE GIVEAGYTLR DKGPRRISPF FIPGRLINLA SGYVSIKYGL RGPNHSVVTA CSTGAHAIGD AARLIALGDA DVMLAGGTES PINRISLAGF SACRALSTCR NDDPERASRP YDVDRDGFVM GEGAAIVVLE ELEHAKKRGA RIYAEIIGYG LSGDAYHITA PSESGEGAQR SMMAALKRAQ VNVSELDYIN AHGTSTMADV IELAAVERVL GYYAPQVSMS STKSSIGHLL GAAGAAEAIF CVLAIRDNIA PATLNLENPS IETKIDLVPH KPRERKIDTV LSNSFGFGGT NASLVMRRFS E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGGCGTGTT GTTATTACTG GTTTAGGATT GGTATCTCCA TTAGCTGGTG ATGTCGAGTA TAGTTGGAAA CGACTTCTTG AAGGAAAAAG TGGTGTTCGA CGCATTACTG AATTTGATGT ATCCGACTTA TCGTGCCAGA TTGCTGCGCG TATCCCCGTA GGGGATGGTA CAAATGGTAC ATATAATGCT GATTTGCATA TGGAATCCAA GGAGCAGCGT AAGGTTGATG CATTTATTGT TTATGCAATT GCAGCCGCTG ACCAAGCACT TGCAGATGCT GAGTGGTTTC CCAAGAGTGA TGAAGACCAG ATTTGCACAG GTGTGTTAAT TGGTTCGGGT ATTGGTGGCA TTGAAGGGAT TGTTGAGGCT GGTTATACGC TTCGTGATAA AGGTCCACGG CGTATTTCTC CTTTTTTTAT TCCAGGACGT TTGATTAATC TTGCTTCTGG TTATGTATCT ATTAAATATG GTTTGCGTGG TCCTAATCAT TCGGTTGTAA CGGCCTGTTC AACAGGTGCA CATGCCATTG GTGATGCAGC ACGTTTGATT GCGTTGGGTG ATGCAGATGT GATGCTAGCA GGTGGCACTG AATCCCCGAT AAATCGAATA TCTCTTGCTG GTTTTTCTGC TTGTCGAGCT CTTTCTACTT GTCGTAATGA TGATCCTGAA CGGGCATCAC GTCCTTATGA TGTTGATCGT GATGGTTTTG TGATGGGGGA AGGAGCAGCG ATTGTTGTTT TAGAAGAGCT TGAACATGCA AAAAAACGAG GTGCTCGTAT TTATGCTGAG ATTATTGGCT ATGGTCTATC TGGTGACGCT TACCACATTA CAGCACCTTC AGAGAGTGGT GAAGGTGCGC AGCGTAGTAT GATGGCTGCT CTTAAGCGTG CACAAGTGAA TGTAAGCGAA CTTGATTACA TTAATGCTCA CGGTACATCG ACGATGGCTG ATGTTATAGA ATTGGCTGCT GTTGAGCGTG TTTTGGGCTA TTATGCACCA CAAGTATCCA TGTCATCAAC AAAGTCATCT ATAGGACATT TACTTGGTGC AGCTGGTGCA GCTGAGGCTA TTTTTTGTGT TTTAGCTATA CGAGATAATA TAGCTCCAGC AACACTTAAT CTTGAAAATC CATCAATTGA AACGAAAATT GACCTTGTTC CACATAAACC GCGCGAGCGA AAAATTGATA CAGTTCTATC CAATTCTTTT GGATTTGGTG GAACAAATGC GTCTTTAGTG ATGCGGCGTT TTTCGGAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA