BaheA.00113.a

3-oxoacyl-(acyl carrier protein) synthase I

CENTER ID:	BaheA.00113.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.00113.a.A1.GE25177	Full length( BaheA.00113.a )		1	406

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_150424
PATRIC ID:	fig\|283166.5.peg.148
RefSeq:	YP_032987.1
UniProt:	A0A0H3LX56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRVVVTGMG IVSAIGNNPG TVLTSLYEAK SGISYAPQYA KLGFRSRVYG KPDINIEELV DRRALRFHGR GTAWNHIAMD QAIADAGLEP HEVSNEHTGI IMGSGGASTQ SIVEAADITR QKSPKRVGPF VVPKAMSSTA SATLATFFKI KGVNYSISSA CATSNHCIGN AYEMIQYGKQ DRIFAGGCED LDWTLSVLFD AMGAMSSKYN DVPEKASRAY DAHRDGFVIA GGAGVLVLEE LECAKARGAK IYGEIVGYGA TSDGHDMVAP SGEGAERCMR MALATVKNKI DYINPHATAT PVGDPPEIEA IRRIFGAGDQ CPPISATKSL TGHSLGAAGV QEAIYTLLMM NHNFICESAH IEELDPAFAD MPIVRERRDH QQLNTVLSNT FGFGGTNATL VFQRYG

NT Sequence

ATGCGTCGAG TTGTTGTAAC CGGAATGGGA ATTGTCTCCG CGATTGGGAA TAACCCAGGA ACCGTTTTAA CCAGTTTATA TGAAGCAAAA TCCGGGATTT CTTACGCACC TCAGTATGCT AAATTAGGCT TCCGTAGTAG AGTTTACGGT AAACCTGATA TTAATATAGA AGAACTTGTT GATAGACGTG CTCTGCGTTT TCATGGACGC GGAACGGCAT GGAATCATAT CGCCATGGAT CAAGCAATTG CTGATGCGGG TTTAGAGCCC CATGAAGTAT CCAATGAACA CACAGGGATT ATTATGGGCT CTGGAGGAGC TTCAACGCAG TCAATCGTTG AAGCTGCTGA CATTACACGT CAAAAAAGTC CAAAGCGTGT TGGCCCCTTT GTCGTACCTA AAGCAATGAG TTCTACAGCA TCCGCAACAC TTGCAACTTT TTTTAAAATT AAAGGAGTGA ACTATTCAAT CTCTTCTGCT TGTGCTACCT CTAATCATTG TATTGGAAAT GCCTATGAGA TGATACAATA TGGCAAACAG GATCGCATTT TTGCTGGTGG CTGCGAAGAT CTGGATTGGA CATTATCTGT TTTGTTTGAT GCCATGGGTG CTATGTCCAG CAAATATAAT GACGTACCAG AAAAAGCTTC TCGTGCTTAT GATGCTCATC GGGATGGATT TGTTATTGCA GGTGGAGCTG GCGTTTTGGT TCTTGAAGAA CTAGAATGCG CTAAAGCACG TGGTGCAAAA ATCTATGGAG AAATTGTTGG TTATGGTGCG ACATCTGATG GGCATGATAT GGTTGCTCCA TCAGGAGAAG GAGCAGAGCG GTGCATGCGC ATGGCACTTG CAACCGTGAA AAATAAAATT GATTACATTA ATCCTCATGC AACAGCAACG CCTGTTGGGG ATCCTCCTGA AATAGAAGCA ATTCGTCGTA TCTTTGGAGC AGGTGATCAA TGTCCACCAA TTTCCGCCAC AAAATCTCTG ACTGGTCATT CCTTGGGAGC AGCAGGTGTT CAAGAAGCAA TCTATACACT GTTAATGATG AATCATAATT TTATTTGCGA AAGTGCCCAT ATTGAAGAGC TTGATCCAGC TTTTGCCGAT ATGCCAATTG TTCGTGAACG CCGTGATCAT CAACAACTTA ATACTGTCTT GTCAAATACC TTTGGCTTTG GCGGTACCAA TGCAACGCTC GTTTTCCAAC GTTATGGA

Details for BaheA.00113.a.A1.GE25177

HARVESTED ON:	5/6/2008
SEQUENCED ON:	7/29/2008
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRRVVVTGM GIVSAIGNNP GTVLTSLYEA KSGISYAPQY AKLGFRSRVY GKPDINIEEL VDRRALRFHG RGTAWNHIAM DQAIADAGLE PHEVSNEHTG IIMGSGGAST QSIVEAADIT RQKSPKRVGP FVVPKAMSST ASATLATFFK IKGVNYSISS ACATSNHCIG NAYEMIQYGK QDRIFAGGCE DLDWTLSVLF DAMGAMSSKY NDVPEKASRA YDAHRDGFVI AGGAGVLVLE ELECAKARGA KIYGEIVGYG ATSDGHDMVA PSGEGAERCM RMALATVKNK IDYINPHATA TPVGDPPEIE AIRRIFGAGD QCPPISATKS LTGHSLGAAG VQEAIYTLLM MNHNFICESA HIEELDPAFA DMPIVRERRD HQQLNTVLSN TFGFGGTNAT LVFQR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtc gagttgttgt aaccggaatg ggaattgtct ccgcgattgg gaataaccca ggaaccgttt taaccagttt atatgaagca aaatccggga tttcttacgc acctcagtat gctaaattag gcttccgtag tagagtttac ggtaaacctg atattaatat agaagaactt gttgatagac gtgctctgcg ttttcatgga cgcggaacgg catggaatca tatcgccatg gatcaagcaa ttgctgatgc gggtttagag ccccatgaag tatccaatga acacacaggg attattatgg gctctggagg agcttcaacg cagtcaatcg ttgaagctgc tgacattaca cgtcaaaaaa gtccaaagcg tgttggcccc tttgtcgtac ctaaagcaat gagttctaca gcatccgcaa cacttgcaac tttttttaaa attaaaggag tgaactattc aatctcttct gcttgtgcta cctctaatca ttgtattgga aatgcctatg agatgataca atatggcaaa caggatcgca tttttgctgg tggctgcgaa gatctggatt ggacattatc tgttttgttt gatgccatgg gtgctatgtc cagcaaatat aatgacgtac cagaaaaagc ttctcgtgct tatgatgctc atcgggatgg atttgttatt gcaggtggag ctggcgtttt ggttcttgaa gaactagaat gcgctaaagc acgtggtgca aaaatctatg gagaaattgt tggttatggt gcgacatctg atgggcatga tatggttgct ccatcaggag aaggagcaga gcggtgcatg cgcatggcac ttgcaaccgt gaaaaataaa attgattaca ttaatcctca tgcaacagca acgcctgttg gggatcctcc tgaaatagaa gcaattcgtc gtatctttgg agcaggtgat caatgtccac caatttccgc cacaaaatct ctgactggtc attccttggg agcagcaggt gttcaagaag caatctatac actgttaatg atgaatcata attttatttg cgaaagtgcc catattgaag agcttgatcc agcttttgcc gatatgccaa ttgttcgtga acgccgtgat catcaacaac ttaatactgt cttgtcaaat acctttggct ttggcggtac caatgcaacg ctcgttttcc aacgtt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtcgagtt gttgtaaccg gaatgggaat tgtctccgcg attgggaata acccaggaac cgttttaacc agtttatatg aagcaaaatc cgggatttct tacgcacctc agtatgctaa attaggcttc cgtagtagag tttacggtaa acctgatatt aatatagaag aacttgttga tagacgtgct ctgcgttttc atggacgcgg aacggcatgg aatcatatcg ccatggatca agcaattgct gatgcgggtt tagagcccca tgaagtatcc aatgaacaca cagggattat tatgggctct ggaggagctt caacgcagtc aatcgttgaa gctgctgaca ttacacgtca aaaaagtcca aagcgtgttg gcccctttgt cgtacctaaa gcaatgagtt ctacagcatc cgcaacactt gcaacttttt ttaaaattaa aggagtgaac tattcaatct cttctgcttg tgctacctct aatcattgta ttggaaatgc ctatgagatg atacaatatg gcaaacagga tcgcattttt gctggtggct gcgaagatct ggattggaca ttatctgttt tgtttgatgc catgggtgct atgtccagca aatataatga cgtaccagaa aaagcttctc gtgcttatga tgctcatcgg gatggatttg ttattgcagg tggagctggc gttttggttc ttgaagaact agaatgcgct aaagcacgtg gtgcaaaaat ctatggagaa attgttggtt atggtgcgac atctgatggg catgatatgg ttgctccatc aggagaagga gcagagcggt gcatgcgcat ggcacttgca accgtgaaaa ataaaattga ttacattaat cctcatgcaa cagcaacgcc tgttggggat cctcctgaaa tagaagcaat tcgtcgtatc tttggagcag gtgatcaatg tccaccaatt tccgccacaa aatctctgac tggtcattcc ttgggagcag caggtgttca agaagcaatc tatacactgt taatgatgaa tcataatttt atttgcgaaa gtgcccatat tgaagagctt gatccagctt ttgccgatat gccaattgtt cgtgaacgcc gtgatcatca acaacttaat actgtcttgt caaatacctt tggctttggc ggtaccaatg caacgctcgt tttccaacgt tatggaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa