BaheA.00010.c
3-ketoacyl-(acyl-carrier-protein) reductase
| CENTER ID: | BaheA.00010.c |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00010.c.A1.GE25173 | Full length( BaheA.00010.c ) | 1 | 245 | ||
| BaheA.00010.c.B1.GE25269 | Full length( BaheA.00010.c ) | 1 | 245 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00010.c.A1.PW25211 | Full length( BaheA.00010.c ) | 1 | 245 |
Structures
3GRP
DEPOSITED: 3/26/2009
DETERMINATION: XRay
CLONE: BaheA.00010.c.A1.GE25173
PROTEIN: BaheA.00010.c.A1.PS00035
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|283166.5.peg.584 |
| RefSeq: | YP_033370.1 |
| UniProt: | A0A0H3LWT6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKLTGRKAL VTGATGGIGE AIARCFHAQG AIVGLHGTRE DKLKEIAADL GKDVFVFSAN LSDRKSIKQL AEVAEREMEG IDILVNNAGI TRDGLFVRMQ DQDWDDVLAV NLTAASTLTR ELIHSMMRRR YGRIINITSI VGVVGNPGQT NYCAAKAGLI GFSKALAQEI ASRNITVNCI APGFIKSAMT DKLNEKQKEA IMAMIPMKRM GIGEEIAFAT VYLASDEAAY LTGQTLHING GMAMI
NT Sequence
ATGTTTAAAT TAACAGGTCG TAAAGCTCTT GTGACGGGAG CAACGGGAGG AATTGGTGAG GCAATTGCGC GTTGTTTCCA TGCACAAGGG GCAATTGTTG GGCTCCATGG AACACGTGAA GACAAGCTGA AAGAAATTGC TGCAGATTTA GGAAAAGATG TTTTTGTGTT CTCTGCCAAT CTTTCTGACC GTAAATCCAT TAAGCAACTG GCTGAAGTTG CGGAAAGAGA AATGGAGGGT ATCGATATCT TGGTTAACAA TGCTGGAATC ACCCGAGATG GTCTTTTTGT TCGTATGCAG GATCAAGACT GGGATGATGT CTTAGCAGTC AATCTAACAG CTGCTTCTAC TTTGACACGT GAATTGATAC ATTCGATGAT GCGCCGTCGC TACGGACGGA TTATTAACAT AACATCTATT GTTGGTGTTG TCGGAAATCC TGGGCAAACG AATTATTGTG CTGCAAAAGC TGGTTTGATA GGTTTTTCTA AAGCATTGGC ACAAGAAATT GCTTCACGAA ATATCACTGT CAATTGTATT GCTCCAGGAT TCATTAAATC TGCAATGACA GACAAACTGA ATGAAAAGCA AAAAGAAGCT ATTATGGCTA TGATTCCAAT GAAACGTATG GGAATAGGGG AAGAAATAGC TTTTGCTACT GTTTATTTAG CGAGTGATGA AGCGGCATAT CTGACAGGTC AAACACTTCA TATCAATGGT GGGATGGCAA TGATC
Details for BaheA.00010.c.A1.GE25173
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLTGRKA LVTGATGGIG EAIARCFHAQ GAIVGLHGTR EDKLKEIAAD LGKDVFVFSA NLSDRKSIKQ LAEVAEREME GIDILVNNAG ITRDGLFVRM QDQDWDDVLA VNLTAASTLT RELIHSMMRR RYGRIINITS IVGVVGNPGQ TNYCAAKAGL IGFSKALAQE IASRNITVNC IAPGFIKSAM TDKLNEKQKE AIMAMIPMKR MGIGEEIAFA TVYLASDEAA YLTGQTLHIN GGMAMI
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaaatta acaggtcgta aagctcttgt gacgggagca acgggaggaa ttggtgaggc aattgcgcgt tgtttccatg cacaaggggc aattgttggg ctccatggaa cacgtgaaga caagctgaaa gaaattgctg cagatttagg aaaagatgtt tttgtgttct ctgccaatct ttctgaccgt aaatccatta agcaactggc tgaagttgcg gaaagagaaa tggagggtat cgatatcttg gttaacaatg ctggaatcac ccgagatggt ctttttgttc gtatgcagga tcaagactgg gatgatgtct tagcagtcaa tctaacagct gcttctactt tgacacgtga attgatacat tcgatgatgc gccgtcgcta cggacggatt attaacataa catctattgt tggtgttgtc ggaaatcctg ggcaaacgaa ttattgtgct gcaaaagctg gtttgatagg tttttctaaa gcattggcac aagaaattgc ttcacgaaat atcactgtca attgtattgc tccaggattc attaaatctg caatgacaga caaactgaat gaaaagcaaa aagaagctat tatggctatg attccaatga aacgtatggg aataggggaa gaaatagctt ttgctactgt ttatttagcg agtgatgaag cggcatatct gacaggtcaa acacttcata tcaatggtgg gatggcaatg atctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLTGRKA LVTGATGGIG EAIARCFHAQ GAIVGLHGTR EDKLKEIAAD LGKDVFVFSA NLSDRKSIKQ LAEVAEREME GIDILVNNAG ITRDGLFVRM QDQDWDDVLA VNLTAASTLT RELIHSMMRR RYGRIINITS IVGVVGNPGQ TNYCAAKAGL IGFSKALAQE IASRNITVNC IAPGFIKSAM TDKLNEKQKE AIMAMIPMKR MGIGEEIAFA TVYLASDEAA YLTGQTLHIN GGMAMI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaaatta acaggtcgta aagctcttgt gacgggagca acgggaggaa ttggtgaggc aattgcgcgt tgtttccatg cacaaggggc aattgttggg ctccatggaa cacgtgaaga caagctgaaa gaaattgctg cagatttagg aaaagatgtt tttgtgttct ctgccaatct ttctgaccgt aaatccatta agcaactggc tgaagttgcg gaaagagaaa tggagggtat cgatatcttg gttaacaatg ctggaatcac ccgagatggt ctttttgttc gtatgcagga tcaagactgg gatgatgtct tagcagtcaa tctaacagct gcttctactt tgacacgtga attgatacat tcgatgatgc gccgtcgcta cggacggatt attaacataa catctattgt tggtgttgtc ggaaatcctg ggcaaacgaa ttattgtgct gcaaaagctg gtttgatagg tttttctaaa gcattggcac aagaaattgc ttcacgaaat atcactgtca attgtattgc tccaggattc attaaatctg caatgacaga caaactgaat gaaaagcaaa aagaagctat tatggctatg attccaatga aacgtatggg aataggggaa gaaatagctt ttgctactgt ttatttagcg agtgatgaag cggcatatct gacaggtcaa acacttcata tcaatggtgg gatggcaatg atcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BaheA.00010.c.B1.GE25269
| HARVESTED ON: | 6/3/2008 |
| SEQUENCED ON: | 9/9/2008 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMF KLTGRKALVT GATGGIGEAI ARCFHAQGAI VGLHGTREDK LKEIAADLGK DVFVFSANLS DRKSIKQLAE VAEREMEGID ILVNNAGITR DGLFVRMQDQ DWDDVLAVNL TAASTLTREL IHSMMRRRYG RIINITSIVG VVGNPGQTNY CAAKAGLIGF SKALAQEIAS RNITVNCIAP GFIKSAMTDK LNEKQKEAIM AMIPMKRMGI GEEIAFATVY LASXEAAYLT GQTLHIXGGM AMI
Validated NT Sequence
ttnnttnntt taagaagnga gatataccat ggctcaccac caccaccacc atatgtttaa attaacaggt cgtaaagctc ttgtgacggg agcaacggga ggaattggtg aggcaattgc gcgttgtttc catgcacaag gggcaattgt tgggctccat ggaacacgtg aagacaagct gaaagaaatt gctgcagatt taggaaaaga tgtttttgtg ttctctgcca atctttctga ccgtaaatcc attaagcaac tggctgaagt tgcggaaaga gaaatggagg gtatcgatat cttggttaac aatgctggaa tcacccgaga tggtcttttt gttcgtatgc aggatcaaga ctgggatgat gtcttagcag tcaatctaac agctgcttct actttgacac gtgaattgat acattcgatg atgcgccgtc gctacggacg gattattaac ataacatcta ttgttggtgt tgtcggaaat cctgggcaaa cgaattattg tgctgcaaaa gctggtttga taggtttttc taaagcattg gcacaagaaa ttgcttcacg aaatatcact gtcaattgta ttgctccagg attcattaaa tctgcaatga cagacaaact gaatgaaaag caaaaagaag ctattatggc tatgattccn atgaaacgta tgggaatagg ggaagaaata gcttttgcta ctgtttattt agcgagtgan gaagcggcat atctgacagg tcaaacactt catatcantg gtgggatggc aatgatctaa gtgagtnaga
Expected Protein Sequence
MAHHHHHHMF KLTGRKALVT GATGGIGEAI ARCFHAQGAI VGLHGTREDK LKEIAADLGK DVFVFSANLS DRKSIKQLAE VAEREMEGID ILVNNAGITR DGLFVRMQDQ DWDDVLAVNL TAASTLTREL IHSMMRRRYG RIINITSIVG VVGNPGQTNY CAAKAGLIGF SKALAQEIAS RNITVNCIAP GFIKSAMTDK LNEKQKEAIM AMIPMKRMGI GEEIAFATVY LASDEAAYLT GQTLHINGGM AMI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aattaacagg tcgtaaagct cttgtgacgg gagcaacggg aggaattggt gaggcaattg cgcgttgttt ccatgcacaa ggggcaattg ttgggctcca tggaacacgt gaagacaagc tgaaagaaat tgctgcagat ttaggaaaag atgtttttgt gttctctgcc aatctttctg accgtaaatc cattaagcaa ctggctgaag ttgcggaaag agaaatggag ggtatcgata tcttggttaa caatgctgga atcacccgag atggtctttt tgttcgtatg caggatcaag actgggatga tgtcttagca gtcaatctaa cagctgcttc tactttgaca cgtgaattga tacattcgat gatgcgccgt cgctacggac ggattattaa cataacatct attgttggtg ttgtcggaaa tcctgggcaa acgaattatt gtgctgcaaa agctggtttg ataggttttt ctaaagcatt ggcacaagaa attgcttcac gaaatatcac tgtcaattgt attgctccag gattcattaa atctgcaatg acagacaaac tgaatgaaaa gcaaaaagaa gctattatgg ctatgattcc aatgaaacgt atgggaatag gggaagaaat agcttttgct actgtttatt tagcgagtga tgaagcggca tatctgacag gtcaaacact tcatatcaat ggtgggatgg caatgatctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaheA.00010.c.A1.PW25211
| PURIFICATION DATe: | 9/13/2010 |
| CONCENTRATION: | 27.03mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLTGRKA LVTGATGGIG EAIARCFHAQ GAIVGLHGTR EDKLKEIAAD LGKDVFVFSA NLSDRKSIKQ LAEVAEREME GIDILVNNAG ITRDGLFVRM QDQDWDDVLA VNLTAASTLT RELIHSMMRR RYGRIINITS IVGVVGNPGQ TNYCAAKAGL IGFSKALAQE IASRNITVNC IAPGFIKSAM TDKLNEKQKE AIMAMIPMKR MGIGEEIAFA TVYLASDEAA YLTGQTLHIN GGMAMI
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaaatta acaggtcgta aagctcttgt gacgggagca acgggaggaa ttggtgaggc aattgcgcgt tgtttccatg cacaaggggc aattgttggg ctccatggaa cacgtgaaga caagctgaaa gaaattgctg cagatttagg aaaagatgtt tttgtgttct ctgccaatct ttctgaccgt aaatccatta agcaactggc tgaagttgcg gaaagagaaa tggagggtat cgatatcttg gttaacaatg ctggaatcac ccgagatggt ctttttgttc gtatgcagga tcaagactgg gatgatgtct tagcagtcaa tctaacagct gcttctactt tgacacgtga attgatacat tcgatgatgc gccgtcgcta cggacggatt attaacataa catctattgt tggtgttgtc ggaaatcctg ggcaaacgaa ttattgtgct gcaaaagctg gtttgatagg tttttctaaa gcattggcac aagaaattgc ttcacgaaat atcactgtca attgtattgc tccaggattc attaaatctg caatgacaga caaactgaat gaaaagcaaa aagaagctat tatggctatg attccaatga aacgtatggg aataggggaa gaaatagctt ttgctactgt ttatttagcg agtgatgaag cggcatatct gacaggtcaa acacttcata tcaatggtgg gatggcaatg atctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLTGRKA LVTGATGGIG EAIARCFHAQ GAIVGLHGTR EDKLKEIAAD LGKDVFVFSA NLSDRKSIKQ LAEVAEREME GIDILVNNAG ITRDGLFVRM QDQDWDDVLA VNLTAASTLT RELIHSMMRR RYGRIINITS IVGVVGNPGQ TNYCAAKAGL IGFSKALAQE IASRNITVNC IAPGFIKSAM TDKLNEKQKE AIMAMIPMKR MGIGEEIAFA TVYLASDEAA YLTGQTLHIN GGMAMI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTAAATTA ACAGGTCGTA AAGCTCTTGT GACGGGAGCA ACGGGAGGAA TTGGTGAGGC AATTGCGCGT TGTTTCCATG CACAAGGGGC AATTGTTGGG CTCCATGGAA CACGTGAAGA CAAGCTGAAA GAAATTGCTG CAGATTTAGG AAAAGATGTT TTTGTGTTCT CTGCCAATCT TTCTGACCGT AAATCCATTA AGCAACTGGC TGAAGTTGCG GAAAGAGAAA TGGAGGGTAT CGATATCTTG GTTAACAATG CTGGAATCAC CCGAGATGGT CTTTTTGTTC GTATGCAGGA TCAAGACTGG GATGATGTCT TAGCAGTCAA TCTAACAGCT GCTTCTACTT TGACACGTGA ATTGATACAT TCGATGATGC GCCGTCGCTA CGGACGGATT ATTAACATAA CATCTATTGT TGGTGTTGTC GGAAATCCTG GGCAAACGAA TTATTGTGCT GCAAAAGCTG GTTTGATAGG TTTTTCTAAA GCATTGGCAC AAGAAATTGC TTCACGAAAT ATCACTGTCA ATTGTATTGC TCCAGGATTC ATTAAATCTG CAATGACAGA CAAACTGAAT GAAAAGCAAA AAGAAGCTAT TATGGCTATG ATTCCAATGA AACGTATGGG AATAGGGGAA GAAATAGCTT TTGCTACTGT TTATTTAGCG AGTGATGAAG CGGCATATCT GACAGGTCAA ACACTTCATA TCAATGGTGG GATGGCAATG ATCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA