BaheA.00010.b
enoyl-(acyl carrier protein) reductase
| CENTER ID: | BaheA.00010.b |
| ORGANISM: | Bartonella henselae str. Houston-1 |
| ASSOCIATED DISEASE: | Bacillary angiomatosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00010.b.A1.GE25172 | Full length( BaheA.00010.b ) | 1 | 272 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaheA.00010.b.A1.PW25210 | Full length( BaheA.00010.b ) | 1 | 272 | ||
| BaheA.00010.b.A1.PW25955 | Full length( BaheA.00010.b ) | 1 | 272 | unavailable | |
| BaheA.00010.b.A1.PW26984 | Full length( BaheA.00010.b ) | 1 | 272 |
Structures
4EIT
DEPOSITED: 4/5/2012
DETERMINATION: XRay
CLONE: BaheA.00010.b.A1.GE25005
PROTEIN: BaheA.00010.b.A1.PW26981
5TF4
DEPOSITED: 9/24/2016
DETERMINATION: XRay
CLONE: BaheA.00010.b.A1.GE25172
PROTEIN: BaheA.00010.b.A1.PW25955
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130466 |
| PATRIC ID: | fig|283166.5.peg.472 |
| RefSeq: | YP_033269.1 |
| UniProt: | A0A0H3M2Q2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKGNGLLYG KRGLILGLAN NRSIAWGIAK TASSAGAELA FTYQGEAMKK RVEPLAEEVK GFVCGHCDVS DSASIDAVFN TIEKKWGKLD FLVHAIGFSD KEELSGRYVD ISESNFMMTM NISVYSLTAL TKRAEKLMSD GGSILTLTYY GAEKVVPNYN VMGVAKAALE ASVKYLAVDL GPKHIRVNAI SAGPIKTLAA SGIGDFRYIL KWNEYNAPLR RTVTIEEVGD SALYLLSDLS RSVTGEVHHV DSGYNIIGMK AVDAPDISVV KE
NT Sequence
ATGGCTAAGG GTAATGGTTT ATTGTATGGC AAGCGTGGTT TAATTTTGGG ATTAGCCAAT AATCGTTCTA TCGCTTGGGG AATAGCTAAA ACAGCAAGTA GCGCGGGTGC AGAGCTTGCT TTTACCTATC AAGGTGAGGC GATGAAAAAG CGTGTTGAGC CTTTGGCTGA AGAAGTAAAG GGTTTTGTTT GTGGTCATTG TGATGTTTCT GATAGTGCAT CCATTGATGC GGTTTTTAAC ACAATAGAGA AGAAATGGGG CAAGCTCGAT TTTTTAGTTC ATGCTATTGG TTTTTCTGAT AAAGAGGAAC TAAGCGGTCG TTATGTCGAT ATAAGCGAAT CGAATTTTAT GATGACCATG AATATTTCTG TTTATTCCTT AACAGCTTTG ACGAAGCGTG CAGAGAAATT GATGTCGGAT GGTGGGTCAA TTTTAACGCT GACTTATTAT GGTGCGGAAA AGGTTGTTCC CAACTATAAC GTGATGGGAG TTGCAAAAGC AGCTCTTGAA GCGAGCGTGA AATATCTGGC AGTGGATTTA GGTCCTAAAC ATATTCGTGT GAATGCTATA TCTGCTGGGC CAATCAAAAC CTTAGCTGCT TCTGGCATTG GTGATTTCCG TTATATTTTA AAATGGAATG AATATAATGC TCCTTTACGT CGTACGGTGA CAATCGAAGA GGTAGGTGAT TCTGCTCTTT ATTTGCTTTC GGATTTGTCT CGTTCTGTTA CCGGTGAAGT GCATCATGTG GATTCAGGTT ATAACATCAT AGGTATGAAA GCTGTTGATG CACCAGATAT TTCCGTCGTT AAAGAA
Details for BaheA.00010.b.A1.GE25172
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV V
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agggtaatgg tttattgtat ggcaagcgtg gtttaatttt gggattagcc aataatcgtt ctatcgcttg gggaatagct aaaacagcaa gtagcgcggg tgcagagctt gcttttacct atcaaggtga ggcgatgaaa aagcgtgttg agcctttggc tgaagaagta aagggttttg tttgtggtca ttgtgatgtt tctgatagtg catccattga tgcggttttt aacacaatag agaagaaatg gggcaagctc gattttttag ttcatgctat tggtttttct gataaagagg aactaagcgg tcgttatgtc gatataagcg aatcgaattt tatgatgacc atgaatattt ctgtttattc cttaacagct ttgacgaagc gtgcagagaa attgatgtcg gatggtgggt caattttaac gctgacttat tatggtgcgg aaaaggttgt tcccaactat aacgtgatgg gagttgcaaa agcagctctt gaagcgagcg tgaaatatct ggcagtggat ttaggtccta aacatattcg tgtgaatgct atatctgctg ggccaatcaa aaccttagct gcttctggca ttggtgattt ccgttatatt ttaaaatgga atgaatataa tgctccttta cgtcgtacgg tgacaatcga agaggtaggt gattctgctc tttatttgct ttcggatttg tctcgttctg ttaccggtga agtgcatcat gtggattcag gttataacat cataggtatg aaagctgttg atgcaccaga tatttccgtc gtta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV VKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaagggt aatggtttat tgtatggcaa gcgtggttta attttgggat tagccaataa tcgttctatc gcttggggaa tagctaaaac agcaagtagc gcgggtgcag agcttgcttt tacctatcaa ggtgaggcga tgaaaaagcg tgttgagcct ttggctgaag aagtaaaggg ttttgtttgt ggtcattgtg atgtttctga tagtgcatcc attgatgcgg tttttaacac aatagagaag aaatggggca agctcgattt tttagttcat gctattggtt tttctgataa agaggaacta agcggtcgtt atgtcgatat aagcgaatcg aattttatga tgaccatgaa tatttctgtt tattccttaa cagctttgac gaagcgtgca gagaaattga tgtcggatgg tgggtcaatt ttaacgctga cttattatgg tgcggaaaag gttgttccca actataacgt gatgggagtt gcaaaagcag ctcttgaagc gagcgtgaaa tatctggcag tggatttagg tcctaaacat attcgtgtga atgctatatc tgctgggcca atcaaaacct tagctgcttc tggcattggt gatttccgtt atattttaaa atggaatgaa tataatgctc ctttacgtcg tacggtgaca atcgaagagg taggtgattc tgctctttat ttgctttcgg atttgtctcg ttctgttacc ggtgaagtgc atcatgtgga ttcaggttat aacatcatag gtatgaaagc tgttgatgca ccagatattt ccgtcgttaa agaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.00010.b.A1.PW25210
| PURIFICATION DATe: | 5/25/2008 |
| CONCENTRATION: | 32mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV V
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agggtaatgg tttattgtat ggcaagcgtg gtttaatttt gggattagcc aataatcgtt ctatcgcttg gggaatagct aaaacagcaa gtagcgcggg tgcagagctt gcttttacct atcaaggtga ggcgatgaaa aagcgtgttg agcctttggc tgaagaagta aagggttttg tttgtggtca ttgtgatgtt tctgatagtg catccattga tgcggttttt aacacaatag agaagaaatg gggcaagctc gattttttag ttcatgctat tggtttttct gataaagagg aactaagcgg tcgttatgtc gatataagcg aatcgaattt tatgatgacc atgaatattt ctgtttattc cttaacagct ttgacgaagc gtgcagagaa attgatgtcg gatggtgggt caattttaac gctgacttat tatggtgcgg aaaaggttgt tcccaactat aacgtgatgg gagttgcaaa agcagctctt gaagcgagcg tgaaatatct ggcagtggat ttaggtccta aacatattcg tgtgaatgct atatctgctg ggccaatcaa aaccttagct gcttctggca ttggtgattt ccgttatatt ttaaaatgga atgaatataa tgctccttta cgtcgtacgg tgacaatcga agaggtaggt gattctgctc tttatttgct ttcggatttg tctcgttctg ttaccggtga agtgcatcat gtggattcag gttataacat cataggtatg aaagctgttg atgcaccaga tatttccgtc gtta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV VKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAGGGT AATGGTTTAT TGTATGGCAA GCGTGGTTTA ATTTTGGGAT TAGCCAATAA TCGTTCTATC GCTTGGGGAA TAGCTAAAAC AGCAAGTAGC GCGGGTGCAG AGCTTGCTTT TACCTATCAA GGTGAGGCGA TGAAAAAGCG TGTTGAGCCT TTGGCTGAAG AAGTAAAGGG TTTTGTTTGT GGTCATTGTG ATGTTTCTGA TAGTGCATCC ATTGATGCGG TTTTTAACAC AATAGAGAAG AAATGGGGCA AGCTCGATTT TTTAGTTCAT GCTATTGGTT TTTCTGATAA AGAGGAACTA AGCGGTCGTT ATGTCGATAT AAGCGAATCG AATTTTATGA TGACCATGAA TATTTCTGTT TATTCCTTAA CAGCTTTGAC GAAGCGTGCA GAGAAATTGA TGTCGGATGG TGGGTCAATT TTAACGCTGA CTTATTATGG TGCGGAAAAG GTTGTTCCCA ACTATAACGT GATGGGAGTT GCAAAAGCAG CTCTTGAAGC GAGCGTGAAA TATCTGGCAG TGGATTTAGG TCCTAAACAT ATTCGTGTGA ATGCTATATC TGCTGGGCCA ATCAAAACCT TAGCTGCTTC TGGCATTGGT GATTTCCGTT ATATTTTAAA ATGGAATGAA TATAATGCTC CTTTACGTCG TACGGTGACA ATCGAAGAGG TAGGTGATTC TGCTCTTTAT TTGCTTTCGG ATTTGTCTCG TTCTGTTACC GGTGAAGTGC ATCATGTGGA TTCAGGTTAT AACATCATAG GTATGAAAGC TGTTGATGCA CCAGATATTT CCGTCGTTAA AGAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BaheA.00010.b.A1.PW25955
| PURIFICATION DATe: | 10/21/2008 |
| CONCENTRATION: | 20.81mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV V
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agggtaatgg tttattgtat ggcaagcgtg gtttaatttt gggattagcc aataatcgtt ctatcgcttg gggaatagct aaaacagcaa gtagcgcggg tgcagagctt gcttttacct atcaaggtga ggcgatgaaa aagcgtgttg agcctttggc tgaagaagta aagggttttg tttgtggtca ttgtgatgtt tctgatagtg catccattga tgcggttttt aacacaatag agaagaaatg gggcaagctc gattttttag ttcatgctat tggtttttct gataaagagg aactaagcgg tcgttatgtc gatataagcg aatcgaattt tatgatgacc atgaatattt ctgtttattc cttaacagct ttgacgaagc gtgcagagaa attgatgtcg gatggtgggt caattttaac gctgacttat tatggtgcgg aaaaggttgt tcccaactat aacgtgatgg gagttgcaaa agcagctctt gaagcgagcg tgaaatatct ggcagtggat ttaggtccta aacatattcg tgtgaatgct atatctgctg ggccaatcaa aaccttagct gcttctggca ttggtgattt ccgttatatt ttaaaatgga atgaatataa tgctccttta cgtcgtacgg tgacaatcga agaggtaggt gattctgctc tttatttgct ttcggatttg tctcgttctg ttaccggtga agtgcatcat gtggattcag gttataacat cataggtatg aaagctgttg atgcaccaga tatttccgtc gtta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV VKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAGGGT AATGGTTTAT TGTATGGCAA GCGTGGTTTA ATTTTGGGAT TAGCCAATAA TCGTTCTATC GCTTGGGGAA TAGCTAAAAC AGCAAGTAGC GCGGGTGCAG AGCTTGCTTT TACCTATCAA GGTGAGGCGA TGAAAAAGCG TGTTGAGCCT TTGGCTGAAG AAGTAAAGGG TTTTGTTTGT GGTCATTGTG ATGTTTCTGA TAGTGCATCC ATTGATGCGG TTTTTAACAC AATAGAGAAG AAATGGGGCA AGCTCGATTT TTTAGTTCAT GCTATTGGTT TTTCTGATAA AGAGGAACTA AGCGGTCGTT ATGTCGATAT AAGCGAATCG AATTTTATGA TGACCATGAA TATTTCTGTT TATTCCTTAA CAGCTTTGAC GAAGCGTGCA GAGAAATTGA TGTCGGATGG TGGGTCAATT TTAACGCTGA CTTATTATGG TGCGGAAAAG GTTGTTCCCA ACTATAACGT GATGGGAGTT GCAAAAGCAG CTCTTGAAGC GAGCGTGAAA TATCTGGCAG TGGATTTAGG TCCTAAACAT ATTCGTGTGA ATGCTATATC TGCTGGGCCA ATCAAAACCT TAGCTGCTTC TGGCATTGGT GATTTCCGTT ATATTTTAAA ATGGAATGAA TATAATGCTC CTTTACGTCG TACGGTGACA ATCGAAGAGG TAGGTGATTC TGCTCTTTAT TTGCTTTCGG ATTTGTCTCG TTCTGTTACC GGTGAAGTGC ATCATGTGGA TTCAGGTTAT AACATCATAG GTATGAAAGC TGTTGATGCA CCAGATATTT CCGTCGTTAA AGAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BaheA.00010.b.A1.PW26984
| PURIFICATION DATe: | 6/1/2009 |
| CONCENTRATION: | 19.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV V
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agggtaatgg tttattgtat ggcaagcgtg gtttaatttt gggattagcc aataatcgtt ctatcgcttg gggaatagct aaaacagcaa gtagcgcggg tgcagagctt gcttttacct atcaaggtga ggcgatgaaa aagcgtgttg agcctttggc tgaagaagta aagggttttg tttgtggtca ttgtgatgtt tctgatagtg catccattga tgcggttttt aacacaatag agaagaaatg gggcaagctc gattttttag ttcatgctat tggtttttct gataaagagg aactaagcgg tcgttatgtc gatataagcg aatcgaattt tatgatgacc atgaatattt ctgtttattc cttaacagct ttgacgaagc gtgcagagaa attgatgtcg gatggtgggt caattttaac gctgacttat tatggtgcgg aaaaggttgt tcccaactat aacgtgatgg gagttgcaaa agcagctctt gaagcgagcg tgaaatatct ggcagtggat ttaggtccta aacatattcg tgtgaatgct atatctgctg ggccaatcaa aaccttagct gcttctggca ttggtgattt ccgttatatt ttaaaatgga atgaatataa tgctccttta cgtcgtacgg tgacaatcga agaggtaggt gattctgctc tttatttgct ttcggatttg tctcgttctg ttaccggtga agtgcatcat gtggattcag gttataacat cataggtatg aaagctgttg atgcaccaga tatttccgtc gtta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKGNGLLY GKRGLILGLA NNRSIAWGIA KTASSAGAEL AFTYQGEAMK KRVEPLAEEV KGFVCGHCDV SDSASIDAVF NTIEKKWGKL DFLVHAIGFS DKEELSGRYV DISESNFMMT MNISVYSLTA LTKRAEKLMS DGGSILTLTY YGAEKVVPNY NVMGVAKAAL EASVKYLAVD LGPKHIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIEEVG DSALYLLSDL SRSVTGEVHH VDSGYNIIGM KAVDAPDISV VKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAGGGT AATGGTTTAT TGTATGGCAA GCGTGGTTTA ATTTTGGGAT TAGCCAATAA TCGTTCTATC GCTTGGGGAA TAGCTAAAAC AGCAAGTAGC GCGGGTGCAG AGCTTGCTTT TACCTATCAA GGTGAGGCGA TGAAAAAGCG TGTTGAGCCT TTGGCTGAAG AAGTAAAGGG TTTTGTTTGT GGTCATTGTG ATGTTTCTGA TAGTGCATCC ATTGATGCGG TTTTTAACAC AATAGAGAAG AAATGGGGCA AGCTCGATTT TTTAGTTCAT GCTATTGGTT TTTCTGATAA AGAGGAACTA AGCGGTCGTT ATGTCGATAT AAGCGAATCG AATTTTATGA TGACCATGAA TATTTCTGTT TATTCCTTAA CAGCTTTGAC GAAGCGTGCA GAGAAATTGA TGTCGGATGG TGGGTCAATT TTAACGCTGA CTTATTATGG TGCGGAAAAG GTTGTTCCCA ACTATAACGT GATGGGAGTT GCAAAAGCAG CTCTTGAAGC GAGCGTGAAA TATCTGGCAG TGGATTTAGG TCCTAAACAT ATTCGTGTGA ATGCTATATC TGCTGGGCCA ATCAAAACCT TAGCTGCTTC TGGCATTGGT GATTTCCGTT ATATTTTAAA ATGGAATGAA TATAATGCTC CTTTACGTCG TACGGTGACA ATCGAAGAGG TAGGTGATTC TGCTCTTTAT TTGCTTTCGG ATTTGTCTCG TTCTGTTACC GGTGAAGTGC ATCATGTGGA TTCAGGTTAT AACATCATAG GTATGAAAGC TGTTGATGCA CCAGATATTT CCGTCGTTAA AGAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA