SSGCID: Target Page

BagrA.18422.a

Cell filamentation protein Fic (VirB-translocated Bep effector protein)

CENTER ID:	BagrA.18422.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.18422.a.B1.GE35696	full length		1	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.1964
RefSeq:	YP_002972613.1
UniProt:	C6AAI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGKYKGSGDP YTDPETGVMY NRFGIKDKAT LQRVESTISY VKSFEFVHTP IRGEFDLGYM KEIHRRLFGD IYEWAGQVRL VDIAKGNSMF ASYNQIESYA PKITQKLAKE QYLCGLGAYD FSQRAGYYMG ELNALHPFRE GNGRTQREFM VQLAREAGYH INWKGIERQE MTRASIEAHF GNSEYLSALI YKNLTKFTEN KRDDVSQGIG KNIAFGVDLN EECLEQQKEK IETPWDREKT SFAKRQKEEL EQLQNDLNVV AKATEKAPYL AAYMDNSNLE QKVLSALRNE KSYIDMFKKL NNHLAFIYRD PQEAALRIEQ TILAGKGDKL PDILEREPDR AGELRGSDRF IDKLKAIGKE RKAALYHVPL AISTMRELQS FYKNSYETHI NRLTRERERL KVEVPSLSQE AVTFMKNVEA GCDSYLKIPE QVSEEFLKLQ SALDKRFGQD AIHKYGFDLS KVISKEQSYN KERIKEFQMV VKFLQQRQQE KINAIARARS RDVIR

NT Sequence

gtggggaagt ataaggggag cggtgatcct tatactgatc ctgaaactgg tgtgatgtat aatcggtttg gtattaaaga caaagccaca ctgcagcgtg tagaatctac gatttcttat gtaaaatctt tcgaatttgt acatactcct attcgtggtg agtttgatct gggttacatg aaagagatcc atagaagact gtttggtgac atatatgaat gggcgggaca agttcgttta gtggatattg ccaaaggcaa tagtatgttt gctagttata atcagattga aagttatgca ccgaagatta cacaaaagct tgcaaaagaa cagtatttgt gtggtcttgg cgcttatgac tttagccaaa gggctgggta ttacatgggt gagttgaatg cattacatcc gtttcgagag gggaatgggc gtactcagcg tgaatttatg gtgcaacttg ctcgtgaagc tggttatcat atcaactgga agggtattga gcgacaagaa atgacacgtg cgtcaattga agcacatttt ggtaattcag aatatctgtc tgcactcatc tataagaacc ttaccaagtt tacagaaaat aaaagggatg atgtttctca aggtattggc aaaaatattg cttttggcgt tgatcttaat gaagagtgct tggaacaaca aaaagagaag attgaaactc cttgggatag agaaaagaca agttttgcta aacgtcaaaa agaagaactt gaacaattac aaaatgattt gaatgttgtt gcaaaagcaa cagaaaaagc gccatacctt gctgcttata tggataactc taatctagaa cagaaggtgc taagcgctct tagaaatgaa aaatcttaca ttgatatgtt taaaaaatta aacaatcatt tagcatttat ttatagagat ccacaagagg ctgcccttag gattgaacaa acaattttag caggtaaagg cgataaattg cctgatattt tggaaaggga acctgataga gcgggtgaac tgcgtgggtc ggatcgtttt attgataaat tgaaggctat aggaaaagaa cgaaaagcag cactttatca tgttcctctc gctatttcta ctatgagaga gcttcaatcc ttttataaga attcttatga gacacatata aataggctta cacgggagcg tgagcgattg aaagttgaag taccctcgct ttcacaagaa gcagtgactt ttatgaaaaa tgttgaagct ggttgtgata gttatttaaa gataccagag caggttagtg aagaattttt aaagctgcaa agtgctttag ataaacgttt tggacaggat gccattcata aatatggttt tgatttatca aaagtgatct ccaaagaaca gtcttataat aaagaacgca taaaagagtt tcaaatggtt gttaaatttt tgcaacagag acaacaggag aaaatcaacg caatagcgcg tgcaagatca agagatgtga tacggtga

Details for BagrA.18422.a.B1.GE35696

HARVESTED ON:	7/10/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	59kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatggtg gggaagtata aggggagcgg tgatccttat actgatcctg aaactggtgt gatgtataat cggtttggta ttaaagacaa agccacactg cagcgtgtag aatctacgat ttcttatgta aaatctttcg aatttgtaca tactcctatt cgtggtgagt ttgatctggg ttacatgaaa gagatccata gaagactgtt tggtgacata tatgaatggg cgggacaagt tcgtttagtg gatattgcca aaggcaatag tatgtttgct agttataatc agattgaaag ttatgcaccg aagattacac aaaagcttgc aaaagaacag tatttgtgtg gtcttggcgc ttatgacttt agccaaaggg ctgggtatta catgggtgag ttgaatgcat tacatccgtt tcgagagggg aatgggcgta ctcagcgtga atttatggtg caacttgctc gtgaagctgg ttatcatatc aactggaagg gtattgagcg acaagaaatg acacgtgcgt caattgaagc acattttggt aattcagaat atctgtctgc actcatctat aagaacctta ccaagtttac agaaaataaa agggatgatg tttctcaagg tattggcaaa aatattgctt ttggcgttga tcttaatgaa gagtgcttgg aacaacaaaa agagaagatt gaaactcctt gnnatagaga aaagacaagt tttgctaaac gtcaaaaaga agaacttgaa caattacaaa atgatttgaa tgttgttgca aaagcaacag aaaaagcgcc ataccttgct gcttatatgg ataactctaa tctagaacag aaggngctaa gcgctcttag aaatgaaaaa tcttacnttg atatgtttaa aaaattnaac nntcatttag catttattta tagaga

Expected Protein Sequence

MAHHHHHHMV GKYKGSGDPY TDPETGVMYN RFGIKDKATL QRVESTISYV KSFEFVHTPI RGEFDLGYMK EIHRRLFGDI YEWAGQVRLV DIAKGNSMFA SYNQIESYAP KITQKLAKEQ YLCGLGAYDF SQRAGYYMGE LNALHPFREG NGRTQREFMV QLAREAGYHI NWKGIERQEM TRASIEAHFG NSEYLSALIY KNLTKFTENK RDDVSQGIGK NIAFGVDLNE ECLEQQKEKI ETPWDREKTS FAKRQKEELE QLQNDLNVVA KATEKAPYLA AYMDNSNLEQ KVLSALRNEK SYIDMFKKLN NHLAFIYRDP QEAALRIEQT ILAGKGDKLP DILEREPDRA GELRGSDRFI DKLKAIGKER KAALYHVPLA ISTMRELQSF YKNSYETHIN RLTRERERLK VEVPSLSQEA VTFMKNVEAG CDSYLKIPEQ VSEEFLKLQS ALDKRFGQDA IHKYGFDLSK VISKEQSYNK ERIKEFQMVV KFLQQRQQEK INAIARARSR DVIR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg ggaagtataa ggggagcggt gatccttata ctgatcctga aactggtgtg atgtataatc ggtttggtat taaagacaaa gccacactgc agcgtgtaga atctacgatt tcttatgtaa aatctttcga atttgtacat actcctattc gtggtgagtt tgatctgggt tacatgaaag agatccatag aagactgttt ggtgacatat atgaatgggc gggacaagtt cgtttagtgg atattgccaa aggcaatagt atgtttgcta gttataatca gattgaaagt tatgcaccga agattacaca aaagcttgca aaagaacagt atttgtgtgg tcttggcgct tatgacttta gccaaagggc tgggtattac atgggtgagt tgaatgcatt acatccgttt cgagagggga atgggcgtac tcagcgtgaa tttatggtgc aacttgctcg tgaagctggt tatcatatca actggaaggg tattgagcga caagaaatga cacgtgcgtc aattgaagca cattttggta attcagaata tctgtctgca ctcatctata agaaccttac caagtttaca gaaaataaaa gggatgatgt ttctcaaggt attggcaaaa atattgcttt tggcgttgat cttaatgaag agtgcttgga acaacaaaaa gagaagattg aaactccttg ggatagagaa aagacaagtt ttgctaaacg tcaaaaagaa gaacttgaac aattacaaaa tgatttgaat gttgttgcaa aagcaacaga aaaagcgcca taccttgctg cttatatgga taactctaat ctagaacaga aggtgctaag cgctcttaga aatgaaaaat cttacattga tatgtttaaa aaattaaaca atcatttagc atttatttat agagatccac aagaggctgc ccttaggatt gaacaaacaa ttttagcagg taaaggcgat aaattgcctg atattttgga aagggaacct gatagagcgg gtgaactgcg tgggtcggat cgttttattg ataaattgaa ggctatagga aaagaacgaa aagcagcact ttatcatgtt cctctcgcta tttctactat gagagagctt caatcctttt ataagaattc ttatgagaca catataaata ggcttacacg ggagcgtgag cgattgaaag ttgaagtacc ctcgctttca caagaagcag tgacttttat gaaaaatgtt gaagctggtt gtgatagtta tttaaagata ccagagcagg ttagtgaaga atttttaaag ctgcaaagtg ctttagataa acgttttgga caggatgcca ttcataaata tggttttgat ttatcaaaag tgatctccaa agaacagtct tataataaag aacgcataaa agagtttcaa atggttgtta aatttttgca acagagacaa caggagaaaa tcaacgcaat agcgcgtgca agatcaagag atgtgatacg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat