BagrA.18414.a

TrwJ4 protein (Trw type IV secretion machinery)

CENTER ID: BagrA.18414.a
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BagrA.18414.a.B2.GE35637 mature protein 23 314
BagrA.18414.a.HZ2.GE41785 mature protein 23 314
BagrA.18414.a.MB2.GE41784 mature protein 23 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|634504.3.peg.2108
RefSeq: YP_002972713.1
UniProt: C6AAT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQKLIIIIAI SAFLGTTNLV RSDTITKQGG AHSRIPIIPG MGSGGLSTSK DYKPEPPKGY KPPSALPPDV KIDLSTLGTM RNRIPSKPSG SKPSNTTPVS PTTQVPKSTF VSAPPAEYLE IINLLKKGEI IELLKKQLEL KKEQLSQVEK AYQAITKSQK TEPQKIDFSS FFLKEPEALY KSDKLSRESY QKVLEEENKI YGSFDQMGKA ISGRLKFASI LDKAVSLETF ENVENRFKYL QHLLDEIQTK ENLKDTADLQ AHIDGSLTMI QNEFIKMEMV AHLRNAEQSL IKRKRRELDM KIFDHEAKRM PTIR
NT Sequence
atgcaaaagc tgattattat catagcaatt agtgcgtttc ttggaacaac aaacttagta agaagtgaca ctattactaa gcaaggagga gctcatagcc gtattcctat aattcctggt atggggtcag gaggtctcag tacaagcaaa gattataaac cagaaccacc aaaaggctat aagccaccat ctgcacttcc acccgatgtt aaaatcgatc tttctacttt gggaacgatg agaaatcgca taccgtccaa acctagtggc tctaagccat ctaacacaac tccagtatct cccacaactc aggtacctaa atcaactttt gtatctgctc cacctgcaga atatctagag attattaatc tattaaaaaa aggggaaatt attgagctgt taaaaaaaca gcttgagttg aaaaaagaac aactttcgca agtagaaaaa gcttatcagg ctataacaaa gagtcagaaa acagaacctc aaaaaataga cttttctagt ttttttctca aagaaccaga agcgctctat aagagtgata agctttcaag ggaatcatat caaaaggtat tagaggaaga aaacaaaatt tatgggtctt ttgatcaaat gggcaaagct atttcggggc gcttaaaatt tgcatcgata ctagataaag ctgtaagttt agaaactttt gaaaacgtag aaaatcgttt taaatacctc caacatctat tagatgaaat tcaaacaaaa gaaaacctga aagatactgc agatcttcaa gcacatatcg atggttcgtt aaccatgatc caaaatgaat ttataaagat ggaaatggta gcgcatttac gtaatgctga gcaatcactt atcaaaagga aaagacgcga acttgatatg aaaatttttg accatgaagc aaaaaggatg cctaccatac gataa
Details for BagrA.18414.a.B2.GE35637
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MGSGGLSTSK DYKPEPPKGY KPPSALPPDV KIDLSTLGTM RNRIPSKPSG SKPSNTTPVS PTTQVPKSTF VSAPPAEYLE IINLLKKGEI IELLKKQLEL KKEQLSQVEK AYQAITKSQK TEPQKIDFSS FFLKEPEALY KSDKLSRESY QKVLEEENKI YGSFDQMGKA ISGRLKFASI LDKAVSLETF ENVENRFKYL QHLLDEIQTK ENLKDTADLQ AHIDGSLTMI QNEFIKMEMV AHLRNAEQSL IKRKRRELDM KIFDHEAKRM PTIR
Validated NT Sequence
tactcactta tcgtatggta ggcatccttt ttgcttcatg gtcaaaaatt ttcatatcaa gttcgcgtct tttccttttg ataagtgatt gctcagcatt acgtaaatgc gctaccattt ccatctttat aaattcattt tggatcatgg ttaacgaacc atcgatatgt gcttgaagat ctgcagtatc tttcaggttt tcttttgttt gaatttcatc taatagatgt tggaggtatt taaaacgatt ttctacgttt tcaaaagttt ctaaacttac agctttatct agtatcgatg caaattttaa gcgccccgaa atagctttgc ccatttgatc aaaagaccca taaattttgt tttcttcctc taataccttt tgatatgatt cccttgaaag cttatcactc ttatagagcg cttctggttc tttgagaaaa aaactagaaa agtctatttt ttgaggttct gttttctgac tctttgttat agcctgataa gctttttcta cttgcgaaag ttgttctttt ttcaactcaa gctgtttttt taacagctca ataatttccc ctttttttaa tagattaata atctctagat attctgcagg tggagcagat acaaaagttg atttaggtac ctgagttgtg ggagatactg gagttgtgtt agatggctta gagccactag gtttggacgg tatgcgattt ctcatcgttc ccaaagtaga aagatcgatt ttaacatcgg gtggaagtgc agatggtggc ttatagcctt ttggtggttc tggtttataa tctttgcttg tactgagacc tcctgacccc ataccaggaa ttataggaat acggctatga gctcctcctt gct
Expected Protein Sequence
MAHHHHHHMD TITKQGGAHS RIPIIPGMGS GGLSTSKDYK PEPPKGYKPP SALPPDVKID LSTLGTMRNR IPSKPSGSKP SNTTPVSPTT QVPKSTFVSA PPAEYLEIIN LLKKGEIIEL LKKQLELKKE QLSQVEKAYQ AITKSQKTEP QKIDFSSFFL KEPEALYKSD KLSRESYQKV LEEENKIYGS FDQMGKAISG RLKFASILDK AVSLETFENV ENRFKYLQHL LDEIQTKENL KDTADLQAHI DGSLTMIQNE FIKMEMVAHL RNAEQSLIKR KRRELDMKIF DHEAKRMPTI R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca ctattactaa gcaaggagga gctcatagcc gtattcctat aattcctggt atggggtcag gaggtctcag tacaagcaaa gattataaac cagaaccacc aaaaggctat aagccaccat ctgcacttcc acccgatgtt aaaatcgatc tttctacttt gggaacgatg agaaatcgca taccgtccaa acctagtggc tctaagccat ctaacacaac tccagtatct cccacaactc aggtacctaa atcaactttt gtatctgctc cacctgcaga atatctagag attattaatc tattaaaaaa aggggaaatt attgagctgt taaaaaaaca gcttgagttg aaaaaagaac aactttcgca agtagaaaaa gcttatcagg ctataacaaa gagtcagaaa acagaacctc aaaaaataga cttttctagt ttttttctca aagaaccaga agcgctctat aagagtgata agctttcaag ggaatcatat caaaaggtat tagaggaaga aaacaaaatt tatgggtctt ttgatcaaat gggcaaagct atttcggggc gcttaaaatt tgcatcgata ctagataaag ctgtaagttt agaaactttt gaaaacgtag aaaatcgttt taaatacctc caacatctat tagatgaaat tcaaacaaaa gaaaacctga aagatactgc agatcttcaa gcacatatcg atggttcgtt aaccatgatc caaaatgaat ttataaagat ggaaatggta gcgcatttac gtaatgctga gcaatcactt atcaaaagga aaagacgcga acttgatatg aaaatttttg accatgaagc aaaaaggatg cctaccatac gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BagrA.18414.a.HZ2.GE41785
HARVESTED ON: 8/7/2017
SEQUENCED ON: data unavailable
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD TITKQGGAHS RIPIIPGMGS GGLSTSKDYK PEPPKGYKPP SALPPDVKID LSTLGTMRNR IPSKPSGSKP SNTTPVSPTT QVPKSTFVSA PPAEYLEIIN LLKKGEIIEL LKKQLELKKE QLSQVEKAYQ AITKSQKTEP QKIDFSSFFL KEPEALYKSD KLSRESYQKV LEEENKIYGS FDQMGKAISG RLKFASILDK AVSLETFENV ENRFKYLQHL LDEIQTKENL KDTADLQAHI DGSLTMIQNE FIKMEMVAHL RNAEQSLIKR KRRELDMKIF DHEAKRM
Validated NT Sequence
aggcatcctt tttgcttcat ggtcaaaaat tttcatatca agttcgcgtc ttttcctttt gataagtgat tgctcagcat tacgtaaatg cgctaccatt tccatcttta taaattcatt ttggatcatg gttaacgaac catcgatatg tgcttgaaga tctgcagtat ctttcaggtt ttcttttgtt tgaatttcat ctaatagatg ttggaggtat ttaaaacgat tttctacgtt ttcaaaagtt tctaaactta cagctttatc tagtatcgat gcaaatttta agcgccccga aatagctttg cccatttgat caaaagaccc ataaattttg ttttcttcct ctaatacctt ttgatatgat tcccttgaaa gcttatcact cttatagagc gcttctggtt ctttgagaaa aaaactagaa aaatctattt tttgaggttc tgttttctga ctctttgtta tagcctgata agctttttct acttgcgaaa gttgttcttt tttcaactca agctgttttt ttaacagctc aataatttcc ccttttttta atagattaat aatctctaga tattctgcag gtggagcaga tacaaaagtt gatttaggta cctgagttgt gggagatact ggagttgtgt tagatggctt agagccacta ggtttggacg gtatgcgatt tctcatcgtt cccaaagtag aaagatcgat tttaacatcg ggtggaagtg cagatggtgg cttatagcct tttggtggtt ctggtttata atctttgctt gtactgagac ctcctgaccc cataccagga attataggaa tacggctatg agctcctcct tgcttagtaa tagtgtccat cgaaccagga ccctgggtct gagcttccag ggtaccacca atctgttctc tgtgagcctc aataatatcg ttatcctcca tgtccaaatc ttcaggggtc tgatcagctt gaattctaat accgtcgtac aagaatctta aggagtccat ttccttaccc tgtcttttag cgaacgcttc catcagcctt cttaaaggag tggtcttttt gatcttgaag aagatctctg aagatccatc ggacaccttt aaattgatgt gagtctcagg cttgacttct ggcttgacct ctggcttagc ttcttgattg acttctgagt ccgacttcat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSDT ITKQGGAHSR IPIIPGMGSG GLSTSKDYKP EPPKGYKPPS ALPPDVKIDL STLGTMRNRI PSKPSGSKPS NTTPVSPTTQ VPKSTFVSAP PAEYLEIINL LKKGEIIELL KKQLELKKEQ LSQVEKAYQA ITKSQKTEPQ KIDFSSFFLK EPEALYKSDK LSRESYQKVL EEENKIYGSF DQMGKAISGR LKFASILDKA VSLETFENVE NRFKYLQHLL DEIQTKENLK DTADLQAHID GSLTMIQNEF IKMEMVAHLR NAEQSLIKRK RRELDMKIFD HEAKRMPTIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg acactattac taagcaagga ggagctcata gccgtattcc tataattcct ggtatggggt caggaggtct cagtacaagc aaagattata aaccagaacc accaaaaggc tataagccac catctgcact tccacccgat gttaaaatcg atctttctac tttgggaacg atgagaaatc gcataccgtc caaacctagt ggctctaagc catctaacac aactccagta tctcccacaa ctcaggtacc taaatcaact tttgtatctg ctccacctgc agaatatcta gagattatta atctattaaa aaaaggggaa attattgagc tgttaaaaaa acagcttgag ttgaaaaaag aacaactttc gcaagtagaa aaagcttatc aggctataac aaagagtcag aaaacagaac ctcaaaaaat agacttttct agtttttttc tcaaagaacc agaagcgctc tataagagtg ataagctttc aagggaatca tatcaaaagg tattagagga agaaaacaaa atttatgggt cttttgatca aatgggcaaa gctatttcgg ggcgcttaaa atttgcatcg atactagata aagctgtaag tttagaaact tttgaaaacg tagaaaatcg ttttaaatac ctccaacatc tattagatga aattcaaaca aaagaaaacc tgaaagatac tgcagatctt caagcacata tcgatggttc gttaaccatg atccaaaatg aatttataaa gatggaaatg gtagcgcatt tacgtaatgc tgagcaatca cttatcaaaa ggaaaagacg cgaacttgat atgaaaattt ttgaccatga agcaaaaagg atgcctacca tacgataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BagrA.18414.a.MB2.GE41784
HARVESTED ON: 8/7/2017
SEQUENCED ON: 9/20/2017
EXPECTED MW: 78kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS DTITKQGGAH SRIPIIPGMG SGGLSTSKDY KPEPPKGYKP PSALPPDVKI DLSTLGTMRN RIPSKPSGSK PSNTTPVSPT TQVPKSTFVS APPAEYLEII NLLKKGEIIE LLKKQLELKK EQLSQVEKAY QAITKSQKTE PQKIDFSSFF LKEPEALYKS DKLSRESYQK VLEEENKIYG SFDQMGKAIS GRLKFASILD KAVSLETFEN VENRFKYLQH LLDEIQTKEN LKDTADLQAH IDGSLTMIQN EFIKMEMVAH LRNAEQSLIK RKRRELDMKI FDHEAKRMPT IR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggacac tattactaag caaggaggag ctcatagccg tattcctata attcctggta tggggtcagg aggtctcagt acaagcaaag attataaacc agaaccacca aaaggctata agccaccatc tgcacttcca cccgatgtta aaatcgatct ttctactttg ggaacgatga gaaatcgcat accgtccaaa cctagtggct ctaagccatc taacacaact ccagtatctc ccacaactca ggtacctaaa tcaacttttg tatctgctcc acctgcagaa tatctagaga ttattaatct attaaaaaaa ggggaaatta ttgagctgtt aaaaaaacag cttgagttga aaaaagaaca actttcgcaa gtagaaaaag cttatcaggc tataacaaag agtcagaaaa cagaacctca aaaaatagac ttttctagtt tttttctcaa agaaccagaa gcgctctata agagtgataa gctttcaagg gaatcatatc aaaaggtatt agaggaagaa aacaaaattt atgggtcttt tgatcaaatg ggcaaagcta tttcggggcg cttaaaattt gcatcgatac tagataaagc tgtaagttta gaaacttttg aaaacgtaga aaatcgtttt aaatacctcc aacatctatt agatgaaatt caaacaaaag aaaacctgaa agatactgca gatcttcaag cacatatcga tggttcgtta accatgatcc aaaatgaatt tataaagatg gaaatggtag cgcatttacg taatgctgag caatcactta tcaaaaggaa aagacgcgaa cttgatatga aaatttttga ccatgaagca aaaaggatgc ctaccatacg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa