BagrA.18412.a

VirB4 protein (VirB type IV secretion machinery)

CENTER ID: BagrA.18412.a
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.18412.a.B1.GE35930 full length 1 784
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|634504.3.peg.1543
RefSeq: YP_002972359.1
UniProt: C6AER5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIMKRETLP EEYIPYIRYI NQHVVALNSR CLMVVMAVEG VNFDTADIDQ LNSLHNQLNT LLKNIADERV ALYSHIIRRR ETIYPEGHFL SSFATTLDEK YKKKMVSQEL YRNDLFISLL WNPAVDKTEQ LVSFFQRLSK ARKTQSEPDE EAIRKLEELS QDLMQGLEEY EVRLLSIYEH EGILFSEQSE FLHQLVGGRR ERIPLTFGTI ASTIYSDRVI FGKEIIEIRH ESNERFAGMF GWKEYPSKTR PGMTDGLLTV PFEFILTQSF VFKSKAAAGA IMSRKQNQMI NAADRASSQI DALDEALDDL ESNRFVLGEH HISLAVFADH PKILTENLSK ARAHLTNGGA VIAREDLGLE AAWWAQLPGN FSYRARSGAI TSRNFAALSP FHSFPVGKLN DNVWGAAVAL LKTQAGSPYY FNFHYGDLGN TFVCGPSGSG KTVIVNFLLA QLQKHNPTMV FFDKDQGAEI FVRAGGGKYK PLKNGQPTGI SPLKGMEYTE KNKIFLRNWI LKLVSAEGQI VTEEERQDIA KAIDSLENLP PTQRSLGALQ LFFDNTSKEG IAIRLQRWIR GNDLGWVFDN DQDDLNLNAQ FIGYDMTDFL DNEEIRRPLM MYLFHRILDL IDGRRIIIVV DEFWKALEDD SFKAFAQDRL KTIRKQNGMM LFATQSPKDA LNSTIAHTII EQCPTQIFFP NQKANYNDYV EAFKLTEREF ELIQSELSRE SRRFLVKQGQ SSVVAELNLR GMNDEIAVLS GTTKNIELMN EIINEYGADP DKWLPIFYQR RKNQ
NT Sequence
atgtcaataa tgaaacggga aactttgcct gaagaatata ttccctatat acgctatatc aaccaacatg tcgttgcttt aaattcacgc tgtttaatgg ttgtgatggc tgttgagggc gtaaattttg acactgcgga tattgatcaa ttaaattctt tacacaacca attaaacacc cttttaaaaa atattgcaga tgaacgggtt gctttatatt ctcacatcat ccgccgtcgt gaaacgatct acccagaagg tcattttctt tcatcttttg caacaacatt agatgagaaa tataaaaaga aaatggtttc gcaagaactg tatagaaatg atctgtttat ttcactgctt tggaatcccg cagtagataa aacagaacaa ctggtttcat tttttcagcg cttgagtaaa gcgaggaaaa cacaatctga acccgatgag gaagctattc gtaaactcga agagttaagt caagacctta tgcaaggctt agaagagtat gaagtccgtc tgctatcaat ctatgaacat gaaggtattt tattttctga acaaagtgaa tttctccacc agctggtggg gggaagacgt gagcgtattc ctctcacatt tggaactatt gcttcaacga tttactcgga tcgtgttatt tttgggaaag aaattatcga aattcgccat gaaagcaatg agcgctttgc tggcatgttt ggatggaaag aatacccttc taaaacacgc cctggtatga ctgatggttt gcttacggta ccatttgaat tcattttaac acaatcattt gtctttaaaa gtaaggcggc cgccggtgcg attatgagcc gcaagcaaaa tcaaatgatc aatgcagctg accgtgctag ttcacaaatt gatgcactcg atgaagcgct agatgattta gaatcaaacc gttttgtttt gggcgagcat cacatttctt tagctgtttt tgctgatcat cccaaaatat tgactgaaaa cttatcaaaa gcacgagccc atttaaccaa tgggggagcc gtaattgcca gagaagattt aggattggaa gcagcatggt gggcacagct tcctggaaac tttagctatc gtgcacgttc aggtgccatt accagcagaa actttgccgc tttatcgccc ttccattctt tccctgttgg caaactaaac gataatgttt ggggagctgc tgtcgcattg ctaaaaacac aggcaggttc accttactat tttaattttc attatggcga tcttggaaat acgtttgttt gtggtccatc gggatctggt aaaacagtga ttgttaattt tcttcttgca caattacaaa aacacaatcc aacaatggta ttttttgaca aagatcaagg agcagagatt tttgtacgag ctggaggagg gaaatataaa cctctaaaaa atgggcaacc cacggggatt tctcccttaa agggcatgga atacactgaa aaaaataaaa tctttcttcg taattggatc ttaaagctgg taagcgctga agggcaaatc gtaacagaag aagagcgaca agatatcgcc aaagccatcg attctttaga aaacttaccc ccaacacaac gctctcttgg tgcacttcaa ctgttttttg acaacacatc aaaagaaggt atcgccatac gactacaacg ctggattaga ggcaatgatt tagggtgggt ttttgataat gatcaagatg atctcaattt aaatgcgcag ttcattggat atgacatgac cgatttctta gacaatgaag aaattcggcg ccccttaatg atgtatctct ttcaccgcat tcttgatttg attgatgggc ggcgcatcat tattgtcgtt gatgaattct ggaaagcttt ggaagatgat tcattcaaag cttttgcgca agatcggcta aaaacaatcc gtaaacaaaa tggcatgatg ctctttgcaa cacaaagccc taaagatgct ttgaactcaa caatcgcgca cacaattatt gagcaatgcc ccacccaaat attttttcca aatcaaaaag caaattacaa tgattatgtc gaagctttca aacttactga gcgtgaattt gaactgatac agtcagaatt aagcagagaa tcccgtcgtt ttctcgtcaa acaaggacaa agttccgtcg ttgcagaact taacttacgt ggaatgaatg atgagatcgc cgttttaagc ggcacaacaa aaaacattga actcatgaat gaaattatca acgaatatgg agcagaccct gacaaatggc tgcccatatt ttatcaaagg agaaaaaatc aatga
Details for BagrA.18412.a.B1.GE35930
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 90kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMS IMKRETLPEE YIPYIRYINQ HVVALNSRCL MVVMAVEGVN FDTADIDQLN SLHNQLNTLL KNIADERVAL YSHIIRRRET IYPEGHFLSS FATTLDEKYK KKMVSQELYR NDLFISLLWN PAVDKIEQLV SFFQRLSKAR KTQSEPDEEA IRKLEELSQD LMQGLEEYEV RLLSIYEHEG ILFSEQSEFL HQLVGGRRER IPLTFGTIAS TIYSDRVIFG KEIIEIRHES NERFAGMFGW KEYPSKTRPG MTDGLLTVPF EFILTQSFVF KSXXAAGAXM SRKXNXMIXA AXXAXXXXXA XXEXLDXXXX XXXXXAXXXX XXXDXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXTXXXXX X
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatgtcaata atgaaacggg aaactttgcc tgaagaatat attccctata tacgctatat caaccaacat gtcgttgctt taaattcacg ctgtttaatg gttgtgatgg ctgttgaggg cgtaaatttt gacactgcgg atattgatca attaaattct ttacacaacc aattaaacac ccttttaaaa aatattgcag atgaacgggt tgctttatat tctcacatca tccgccgtcg tgaaacgatc tacccagaag gtcattttct ttcatctttt gcaacaacat tagatgagaa atataaaaag aaaatggttt cgcaagaact gtatagaaat gatctgttta tttcactgct ttggaatccc gcagtagata aaatagaaca actggtttca ttttttcagc gcttgagtaa agcgaggaaa acacaatctg aacccgatga ggaagctatt cgtaaactcg aagagttaag tcaagacctt atgcaaggct tagaagagta tgaagtccgt ctgctatcaa tctatgaaca tgaaggtatt ttattttctg aacaaagtga atttctccac cagctggtgg ggggaagacg tgagcgtatt cctctcacat ttggaactat tgcttcaacg atttactcgg atcgtgttat ttttgggaaa gaaattatcg aaattcgcca tgaaagcaat gagcgctttg ctggcatgtt tggatggaaa gaataccctt ctaaaacacg ccctggtatg actgatggtt tgcttacggt accatttgaa ttcattttaa cacaatcatt tgtctttaaa agtanngngg ccgccggtgc gatnatgagc cgcaagcnaa atcnaatgat cantgcagct ganngtgcta nttnnnnaan ngntgcnntc gangaancnc tagatgantn annnnnannn nnttnnnttt nggcnancat nnnnnnnntn ancnctgatc ntncnnannn nnacngnnan tnnnnnannn nnnnnnnnnn nnnnnnnnna tnnnnnnnna ngnnnnnnnn antnnngnnn nnnnnnnnna cnnnnnnnnn tnnnnnncnn nt
Expected Protein Sequence
MAHHHHHHMS IMKRETLPEE YIPYIRYINQ HVVALNSRCL MVVMAVEGVN FDTADIDQLN SLHNQLNTLL KNIADERVAL YSHIIRRRET IYPEGHFLSS FATTLDEKYK KKMVSQELYR NDLFISLLWN PAVDKTEQLV SFFQRLSKAR KTQSEPDEEA IRKLEELSQD LMQGLEEYEV RLLSIYEHEG ILFSEQSEFL HQLVGGRRER IPLTFGTIAS TIYSDRVIFG KEIIEIRHES NERFAGMFGW KEYPSKTRPG MTDGLLTVPF EFILTQSFVF KSKAAAGAIM SRKQNQMINA ADRASSQIDA LDEALDDLES NRFVLGEHHI SLAVFADHPK ILTENLSKAR AHLTNGGAVI AREDLGLEAA WWAQLPGNFS YRARSGAITS RNFAALSPFH SFPVGKLNDN VWGAAVALLK TQAGSPYYFN FHYGDLGNTF VCGPSGSGKT VIVNFLLAQL QKHNPTMVFF DKDQGAEIFV RAGGGKYKPL KNGQPTGISP LKGMEYTEKN KIFLRNWILK LVSAEGQIVT EEERQDIAKA IDSLENLPPT QRSLGALQLF FDNTSKEGIA IRLQRWIRGN DLGWVFDNDQ DDLNLNAQFI GYDMTDFLDN EEIRRPLMMY LFHRILDLID GRRIIIVVDE FWKALEDDSF KAFAQDRLKT IRKQNGMMLF ATQSPKDALN STIAHTIIEQ CPTQIFFPNQ KANYNDYVEA FKLTEREFEL IQSELSRESR RFLVKQGQSS VVAELNLRGM NDEIAVLSGT TKNIELMNEI INEYGADPDK WLPIFYQRRK NQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa taatgaaacg ggaaactttg cctgaagaat atattcccta tatacgctat atcaaccaac atgtcgttgc tttaaattca cgctgtttaa tggttgtgat ggctgttgag ggcgtaaatt ttgacactgc ggatattgat caattaaatt ctttacacaa ccaattaaac acccttttaa aaaatattgc agatgaacgg gttgctttat attctcacat catccgccgt cgtgaaacga tctacccaga aggtcatttt ctttcatctt ttgcaacaac attagatgag aaatataaaa agaaaatggt ttcgcaagaa ctgtatagaa atgatctgtt tatttcactg ctttggaatc ccgcagtaga taaaacagaa caactggttt cattttttca gcgcttgagt aaagcgagga aaacacaatc tgaacccgat gaggaagcta ttcgtaaact cgaagagtta agtcaagacc ttatgcaagg cttagaagag tatgaagtcc gtctgctatc aatctatgaa catgaaggta ttttattttc tgaacaaagt gaatttctcc accagctggt ggggggaaga cgtgagcgta ttcctctcac atttggaact attgcttcaa cgatttactc ggatcgtgtt atttttggga aagaaattat cgaaattcgc catgaaagca atgagcgctt tgctggcatg tttggatgga aagaataccc ttctaaaaca cgccctggta tgactgatgg tttgcttacg gtaccatttg aattcatttt aacacaatca tttgtcttta aaagtaaggc ggccgccggt gcgattatga gccgcaagca aaatcaaatg atcaatgcag ctgaccgtgc tagttcacaa attgatgcac tcgatgaagc gctagatgat ttagaatcaa accgttttgt tttgggcgag catcacattt ctttagctgt ttttgctgat catcccaaaa tattgactga aaacttatca aaagcacgag cccatttaac caatggggga gccgtaattg ccagagaaga tttaggattg gaagcagcat ggtgggcaca gcttcctgga aactttagct atcgtgcacg ttcaggtgcc attaccagca gaaactttgc cgctttatcg cccttccatt ctttccctgt tggcaaacta aacgataatg tttggggagc tgctgtcgca ttgctaaaaa cacaggcagg ttcaccttac tattttaatt ttcattatgg cgatcttgga aatacgtttg tttgtggtcc atcgggatct ggtaaaacag tgattgttaa ttttcttctt gcacaattac aaaaacacaa tccaacaatg gtattttttg acaaagatca aggagcagag atttttgtac gagctggagg agggaaatat aaacctctaa aaaatgggca acccacgggg atttctccct taaagggcat ggaatacact gaaaaaaata aaatctttct tcgtaattgg atcttaaagc tggtaagcgc tgaagggcaa atcgtaacag aagaagagcg acaagatatc gccaaagcca tcgattcttt agaaaactta cccccaacac aacgctctct tggtgcactt caactgtttt ttgacaacac atcaaaagaa ggtatcgcca tacgactaca acgctggatt agaggcaatg atttagggtg ggtttttgat aatgatcaag atgatctcaa tttaaatgcg cagttcattg gatatgacat gaccgatttc ttagacaatg aagaaattcg gcgcccctta atgatgtatc tctttcaccg cattcttgat ttgattgatg ggcggcgcat cattattgtc gttgatgaat tctggaaagc tttggaagat gattcattca aagcttttgc gcaagatcgg ctaaaaacaa tccgtaaaca aaatggcatg atgctctttg caacacaaag ccctaaagat gctttgaact caacaatcgc gcacacaatt attgagcaat gccccaccca aatatttttt ccaaatcaaa aagcaaatta caatgattat gtcgaagctt tcaaacttac tgagcgtgaa tttgaactga tacagtcaga attaagcaga gaatcccgtc gttttctcgt caaacaagga caaagttccg tcgttgcaga acttaactta cgtggaatga atgatgagat cgccgtttta agcggcacaa caaaaaacat tgaactcatg aatgaaatta tcaacgaata tggagcagac cctgacaaat ggctgcccat attttatcaa aggagaaaaa atcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat