SSGCID: Target Page

BagrA.18411.a

VirB10 protein (VirB type IV secretion machinery)

CENTER ID:	BagrA.18411.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.18411.a.B2.GE35929	soluble domain		52	386

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.18411.a.B2.PS01683	soluble domain		52	386

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.1549
RefSeq:	YP_002972365.1
UniProt:	C6AES1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNTVDEKSI NDRDTIKNAQ GKSQQNNVGK AIALIILFSV CLYLAYSTLV TEKKQPIESS KEGKVIKQTE LFRPAKPTPS PLEQPQRNNA LLPKVELPTP NINQLNSDDS LLEAAQRAPV LAYANTQQGK IDSQTNNGIL PHQLESKPDE TTQRFNHLLK PTVLEGIRAS TLGNRNYIIA MGTSIPCILE TAINSDQQGF ASCIVSRDIL SDNGRVVLLD KGTQIVGEYR AGLKKGQTRL FVLWNRAKTP NGIIISLASP ATDSLGRSGI DGDIDNHWLE RIGSALLVSL IKDATNYANK RLAKKQEKEE TETLSSGQNI ANILVENYAN IPPTLSKNQG EMVNIFVARD LDFSSVYKLK VIENKKQITN RAVSRNFYKN SVIPLK

NT Sequence

atgaataaca ccgtggatga aaaaagcatc aatgatcgcg atacaataaa aaatgctcaa ggaaaaagtc aacagaacaa tgtagggaaa gctattgctc ttattattct tttcagtgtc tgtctttatt tagcctattc aacacttgtt acagaaaaaa aacaaccaat tgaatcttca aaagaaggaa aagttataaa acaaacagag ctttttcgcc ctgcaaagcc taccccttcc ccccttgaac agcctcaacg aaataacgct ctattgccaa aagtcgagct accaacacca aacatcaatc aattaaattc tgatgactca cttctggaag cagcacaacg tgcccctgta ttagcttatg caaatacaca acaaggcaaa atagattcac aaaccaataa tggtattttg cctcaccaac ttgaaagtaa acctgatgaa acgacacaac gttttaatca tcttctcaaa cccacagtgc ttgaaggtat tcgagcttca acccttggca atcgaaacta catcatcgcg atgggaactt ctatcccttg tattttagaa acagccatca acagtgatca acaaggtttt gccagttgta tcgtctccag agatatctta tcagacaatg gtcgtgttgt ccttctcgat aaaggtaccc aaattgttgg tgaatatcgc gccggcttga aaaaaggcca aacacgtctc tttgtgctat ggaatagagc aaaaacccct aatggtatca ttatatcctt agcttcacct gcaacagaca gtttagggcg ttctggtata gatggagata ttgataacca ttggttagag aggattggat ctgcgcttct tgtctccctg ataaaagatg cgacaaacta tgccaataaa cgtttagcaa aaaaacaaga aaaagaagaa actgaaacac tctcttcagg acaaaacatc gcaaacattc tcgtcgaaaa ttacgccaat attcctccaa cattatccaa aaaccaaggg gaaatggtaa atatctttgt tgcccgcgat ttggattttt ccagcgtcta taaattgaaa gtcatcgaaa ataaaaaaca gattaccaat cgagctgttt caagaaactt ttacaaaaat tctgtgatac ctttaaaatg a

Details for BagrA.18411.a.B2.GE35929

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MEKKQPIESS KEGKVIKQTE LFRPAKPTPS PLEQPQRNNA LLPKVELPTP NINQLNSDDS LLEAAQRAPV LAYANTQQGK IDSQTNNGIL PHQLESKPDE TTQRFNHLLK PTVLEGIRAS TLGNRNYIIA MGTSIPCILE TAINSDQQGF ASCIVSRDIL SDNGRVVLLD KGTQIVGEYR AGLKKGQTRL FVLWNRAKTP NGIIISLASP ATDSLGRSGI DGDIDNHWLE RIGSALLVSL IKDATNYANK RLAKKQEKEE TETLSSGQNI ANILVENYAN IPPTLSKNQG EMVNIFVARD LDFSSVYKLK VIENKKQITN RAVSRNFY

Validated NT Sequence

tttgtaaaag tttcttgaaa cagctcgatt ggtaatctgt tttttatttt cgatgacttt caatttatag acgctggaaa aatccaaatc gcgggcaaca aagatattta ccatttcccc ttggtttttg gataatgttg gaggaatatt ggcgtaattt tcgacgagaa tgtttgcgat gttttgtcct gaagagagtg tttcagtttc ttctttttct tgtttttttg ctaaacgttt attggcatag tttgtcgcat cttttatcag ggagacaaga agcgcagatc caatcctctc taaccaatgg ttatcaatat ctccatctat accagaacgc cctaaactgt ctgttgcagg tgaagctaag gatataatga taccattagg ggtttttgct ctattccata gcacaaagag acgtgtttgg ccttttttca agccggcgcg atattcacca acaatttggg tacctttatc gagaaggaca acacgaccat tgtctgataa gatatctctg gagacgatac aactggcaaa accttgttga tcactgttga tggctgtttc taaaatacaa gggatagaag ttcccatcgc gatgatgtag tttcgattgc caagggttga agctcgaata ccttcaagca ctgtgggttt gagaagatga ttaaaacgtt gtgtcgtttc atcaggttta ctttcaagtt ggtgaggcaa aataccatta ttggtttgtg aatctatttt gccttgttgt gtatttgcat aagctaatac aggggcacgt tgtgctgctt ccagaagtga gtcatcagaa tttaattgat tgatgtttgg tgttggtagc tcgacttttg gcaatagagc gttatttcgt tgaggctgtt caagggggga aggggtaggc tttgcagggc gaaaaagctc tgtttgtttt ataacttttc cttcttttga agattcaatt ggttgttttt tttccatatg gtggtggtgg tgg

Expected Protein Sequence

MAHHHHHHME KKQPIESSKE GKVIKQTELF RPAKPTPSPL EQPQRNNALL PKVELPTPNI NQLNSDDSLL EAAQRAPVLA YANTQQGKID SQTNNGILPH QLESKPDETT QRFNHLLKPT VLEGIRASTL GNRNYIIAMG TSIPCILETA INSDQQGFAS CIVSRDILSD NGRVVLLDKG TQIVGEYRAG LKKGQTRLFV LWNRAKTPNG IIISLASPAT DSLGRSGIDG DIDNHWLERI GSALLVSLIK DATNYANKRL AKKQEKEETE TLSSGQNIAN ILVENYANIP PTLSKNQGEM VNIFVARDLD FSSVYKLKVI ENKKQITNRA VSRNFYKNSV IPLK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaaaacaacc aattgaatct tcaaaagaag gaaaagttat aaaacaaaca gagctttttc gccctgcaaa gcctacccct tccccccttg aacagcctca acgaaataac gctctattgc caaaagtcga gctaccaaca ccaaacatca atcaattaaa ttctgatgac tcacttctgg aagcagcaca acgtgcccct gtattagctt atgcaaatac acaacaaggc aaaatagatt cacaaaccaa taatggtatt ttgcctcacc aacttgaaag taaacctgat gaaacgacac aacgttttaa tcatcttctc aaacccacag tgcttgaagg tattcgagct tcaacccttg gcaatcgaaa ctacatcatc gcgatgggaa cttctatccc ttgtatttta gaaacagcca tcaacagtga tcaacaaggt tttgccagtt gtatcgtctc cagagatatc ttatcagaca atggtcgtgt tgtccttctc gataaaggta cccaaattgt tggtgaatat cgcgccggct tgaaaaaagg ccaaacacgt ctctttgtgc tatggaatag agcaaaaacc cctaatggta tcattatatc cttagcttca cctgcaacag acagtttagg gcgttctggt atagatggag atattgataa ccattggtta gagaggattg gatctgcgct tcttgtctcc ctgataaaag atgcgacaaa ctatgccaat aaacgtttag caaaaaaaca agaaaaagaa gaaactgaaa cactctcttc aggacaaaac atcgcaaaca ttctcgtcga aaattacgcc aatattcctc caacattatc caaaaaccaa ggggaaatgg taaatatctt tgttgcccgc gatttggatt tttccagcgt ctataaattg aaagtcatcg aaaataaaaa acagattacc aatcgagctg tttcaagaaa cttttacaaa aattctgtga tacctttaaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BagrA.18411.a.B2.PS01683

PURIFICATION DATe:	11/13/2012
CONCENTRATION:	37.5mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA AAAAACAACC AATTGAATCT TCAAAAGAAG GAAAAGTTAT AAAACAAACA GAGCTTTTTC GCCCTGCAAA GCCTACCCCT TCCCCCCTTG AACAGCCTCA ACGAAATAAC GCTCTATTGC CAAAAGTCGA GCTACCAACA CCAAACATCA ATCAATTAAA TTCTGATGAC TCACTTCTGG AAGCAGCACA ACGTGCCCCT GTATTAGCTT ATGCAAATAC ACAACAAGGC AAAATAGATT CACAAACCAA TAATGGTATT TTGCCTCACC AACTTGAAAG TAAACCTGAT GAAACGACAC AACGTTTTAA TCATCTTCTC AAACCCACAG TGCTTGAAGG TATTCGAGCT TCAACCCTTG GCAATCGAAA CTACATCATC GCGATGGGAA CTTCTATCCC TTGTATTTTA GAAACAGCCA TCAACAGTGA TCAACAAGGT TTTGCCAGTT GTATCGTCTC CAGAGATATC TTATCAGACA ATGGTCGTGT TGTCCTTCTC GATAAAGGTA CCCAAATTGT TGGTGAATAT CGCGCCGGCT TGAAAAAAGG CCAAACACGT CTCTTTGTGC TATGGAATAG AGCAAAAACC CCTAATGGTA TCATTATATC CTTAGCTTCA CCTGCAACAG ACAGTTTAGG GCGTTCTGGT ATAGATGGAG ATATTGATAA CCATTGGTTA GAGAGGATTG GATCTGCGCT TCTTGTCTCC CTGATAAAAG ATGCGACAAA CTATGCCAAT AAACGTTTAG CAAAAAAACA AGAAAAAGAA GAAACTGAAA CACTCTCTTC AGGACAAAAC ATCGCAAACA TTCTCGTCGA AAATTACGCC AATATTCCTC CAACATTATC CAAAAACCAA GGGGAAATGG TAAATATCTT TGTTGCCCGC GATTTGGATT TTTCCAGCGT CTATAAATTG AAAGTCATCG AAAATAAAAA ACAGATTACC AATCGAGCTG TTTCAAGAAA CTTTTACAAA AATTCTGTGA TACCTTTAAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT