BagrA.18408.a

TrwK protein (Trw type IV secretion machinery)

CENTER ID: BagrA.18408.a
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.18408.a.B1.GE35928 full length 1 823
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|634504.3.peg.2099
RefSeq: YP_002972703.1
UniProt: C6AAS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVESSKRL ASETPVSLFL PYSHHITDTI ISTKNAEYLS IWKIDGRSHQ SASEEDIFQW IKELNNTLRG IASANLSFWT HIVRRRVYEY PDSTFDQMFC YQLDEKYRQS FTGYNLMVND LYLTVIFQPI ADKIMSFFSK HERETLDQKK MRQDSCIKEL NDINRTLGQS LNRYGAELLG AYEKNGHAYS SALEFLGMLV NGEYRPMPIC HDRFSDYMSI NRPFFSKWGA LGELRTTSGM RRFGMLEIKE YDATTKPGQL NVLLESDFEF VLTQSFSVLS RHAAKDYLQR HQRNLIDARD VATSQIEQID EALNQLISGH FVMGEHHCTL TIYGNTIQQV RDHMAKASAA LLDVAVLPKP VDLAIEAGYW AQLPGNWKWR PRPAPITSLN FLSFSSFHNF MSGKPTGNPW GPAVTILKTT SKTPLYFNFH ASKLEEDSTD KRLLGNTALI GQSGSGKTVL LGFLVAQAQK FKPTTVVFDK DRGMEIAIRA MGGKYLTFKP GRKSGFNPFQ LPPTQENLIF LKQFVKKLAE AGGEVTHHDE EEINKAVMSV MSSNIDQSLR RLSFLIQFLP NPRLSDYNAH PSVHARLLKW CEGGDYGWLF DNPHDALDLS THQIYGFDIT EFLDNFETRT PVMMYLLYRT EGMISGQRFM YIFDEFWKPL QDPYFEDLAK NKQKTIRKQN GIFVYATQEP SDALESNIAK TLIQQCATYI FLANPKANYE DYTKGFKLTD TEFELVKGLG EFSRQFLIKQ GDQSALAELN LGKFHLTSGG KTIEHDFSDE LLVLSGTPDN AELAESIIAE VGDDPAIWLP IFLQHAKNDR RET
NT Sequence
atgacagctg ttgaaagtag caaacgcctt gcatcagaga cacctgtaag cctgtttctg ccttattcac atcatattac agatacgatt atctctacta aaaatgcgga atatttatcg atttggaaga ttgacgggcg ttcacatcaa agcgcttcag aagaagatat ttttcaatgg ataaaagagc ttaacaatac tctacggggt attgcatcag ccaatttatc attttggaca catattgtgc gtcgccgtgt ttatgaatat cctgattcaa catttgatca aatgttttgc tatcaacttg atgaaaaata tcggcaaagc tttactggtt ataatttgat ggtcaatgat ttatatctaa ctgtcatttt tcaaccgata gcggataaaa ttatgtcgtt cttttctaaa catgaacgtg aaacgcttga tcaaaaaaag atgcgacaag actcatgtat taaagaactc aatgacatca atcgtacttt aggccaatct ttaaaccgtt acggtgcaga acttcttggt gcttatgaaa aaaatgggca tgcttattct tctgcacttg agttccttgg aatgcttgtc aatggtgaat atcgccccat gccaatttgc catgaccgtt tttctgacta tatgtctatc aatcggcctt ttttctcaaa atggggtgct cttggtgaat tgcgaacaac cagtggaatg cgtcgatttg gaatgctgga aattaaagaa tatgatgcaa caaccaaacc aggacaatta aatgttttgt tggaaagtga cttcgagttc gtattgacgc agagcttttc ggtgctctcc cgacatgctg ctaaagacta tttacaacga catcagcgta accttattga tgcacgagat gtggcaacga gccaaattga acaaattgac gaagcactaa accagcttat tagcgggcat tttgtcatgg gagaacatca ctgcacattg actatctatg gtaatacaat tcaacaagtt cgcgaccata tggctaaagc aagtgcagca ttactagacg ttgcggtact gcccaaacct gtagacttag cgatagaggc tggctattgg gcacaacttc ctggcaattg gaaatggaga ccacgtcctg ccccgattac atcattgaac tttttgtcat tttcatcttt tcataatttt atgtcaggaa aacccactgg caacccatgg ggtccagccg tcacaattct taaaacaacc agtaaaacac cgctttattt caactttcat gcctccaaac ttgaagagga ctcaacagat aaacgtttgc tcggtaatac cgcacttatc ggacaatctg gttccggtaa aaccgtatta ctcggctttt tggtagcaca agcacaaaaa tttaagccga caacggttgt ttttgataaa gatcgtggta tggaaattgc cattcgagcg atgggtggaa aatatttaac attcaagcca ggcaggaaaa gtggtttcaa tcctttccaa ctcccaccca cacaagaaaa tctgattttt ctgaaacaat ttgttaaaaa attagcagaa gctggtggtg aggtcacgca tcacgacgag gaagaaatta acaaagcggt tatgtctgtt atgagcagta acattgacca atcactccgt cgtttgtcct ttctgataca atttttacca aatccacgtt taagtgatta taatgctcac ccatctgttc atgctcgctt actaaaatgg tgtgaaggtg gtgattatgg atggttattt gataatcccc atgatgctct tgatctctca acacatcaaa tttacggctt tgatatcacg gaatttttag ataattttga aactcgcacc ccagtgatga tgtatttgct ttaccgcaca gaaggcatga ttagcggaca gcgatttatg tatatctttg atgagttttg gaagcctttg caagatccgt actttgaaga tttggcgaaa aataagcaaa agactatccg taaacaaaat ggtatttttg tttacgcaac acaagagcct agcgatgcct tggaaagtaa tattgccaaa acactcattc aacagtgtgc gacttacatt tttctagcca accccaaggc aaactatgaa gattatacaa aaggctttaa actaacagat acagaatttg aactcgttaa aggacttggt gagtttagtc gccaattcct cattaagcag ggtgatcaat ctgcgcttgc agaactgaat cttggtaaat tccatctgac aagcggtgga aaaaccatcg aacacgactt tagtgacgag ctcttggtgt tatcaggtac accagataac gcagaattag ccgaaagcat tatcgcggaa gttggtgatg atccggcaat atggttacca attttcttac agcacgcaaa aaatgacagg agggaaacat ga
Details for BagrA.18408.a.B1.GE35928
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 94kDa
OBSERVED MW: 94kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 38
Validated AA Sequence
MAHHHHHHMT AVESSKRLAS ETPVSLFLPY SHHITDTIIS TKNAEYLSIW KIDGRSHQSA SEEDIFQWIK ELNNTLRGIA SANLSFWTHI VRRRVYEYPD STFDQMFCYQ LDEKYRQSFT GYNLMVNDLY LTVIFQPIAD KIMSFFSKHE RETLDQKKMR QDSCIKELND INRTLGQSLN RYGAELLGAY EKNGHAYSSA LEFLGMLVNG EYRPMPICHD RFSDYMSINR PFFSKWGALG ELRTTSGMRR FGMLEIKEYD ATTKPXQLNV LLESDFEFVL TXXFSVLSRX AAKXYLQRXQ RNXXXARDXX RAXXXX
Validated NT Sequence
tttgtttact ttaagaagga gatataccat ggctcaccac caccaccacc atatgacagc tgttgaaagt agcaaacgcc ttgcatcaga gacacctgta agcctgtttc tgccttattc acatcatatt acagatacga ttatctctac taaaaatgcg gaatatttat cgatttggaa gattgacggg cgttcacatc aaagcgcttc agaagaagat atttttcaat ggataaaaga gcttaacaat actctacggg gtattgcatc agccaattta tcattttgga cacatattgt gcgtcgccgt gtttatgaat atcctgattc aacatttgat caaatgtttt gctatcaact tgatgaaaaa tatcggcaaa gctttactgg ttataatttg atggtcaatg atttatatct aactgtcatt tttcaaccga tagcggataa aattatgtcg ttcttttcta aacatgaacg tgaaacgctt gatcaaaaaa agatgcgaca agactcatgt attaaagaac tcaatgacat caatcgtact ttaggccaat ctttaaaccg ttacggtgca gaacttcttg gtgcttatga aaaaaatggg catgcttatt cttctgcact tgagttcctt ggaatgcttg tcaatggtga atatcgcccc atgccaattt gccatgaccg tttttctgac tatatgtcta tcaatcggcc ttttttctca aaatggggtg ctcttggtga attgcgaaca accagtggaa tgcgtcgatt tggaatgctg gaaattaaag aatatgatgc aacaaccaaa ccnngacaat taaatgtttt gttggaaagt gacttcgagt tcgtattgac gcnnancttt tcggtgctct cccgacntgc tgctaaanac tatttacaac gacntcagcg taacnnnant gnngcacgag atgngcnncg agccnannga ncaaantgac naancnctaa accagctnna ncnggcantt nncatggnan anatcnnngc ntngacnatc tangnnnnnn nnnnnnnnnn nnnnnnnngn tnnnnaancn tnctnnnnnn nnngnncnnn cnnnnnnnnn annnnnnnnn nnnnngnnnn tnnnnnannn nnntt
Expected Protein Sequence
MAHHHHHHMT AVESSKRLAS ETPVSLFLPY SHHITDTIIS TKNAEYLSIW KIDGRSHQSA SEEDIFQWIK ELNNTLRGIA SANLSFWTHI VRRRVYEYPD STFDQMFCYQ LDEKYRQSFT GYNLMVNDLY LTVIFQPIAD KIMSFFSKHE RETLDQKKMR QDSCIKELND INRTLGQSLN RYGAELLGAY EKNGHAYSSA LEFLGMLVNG EYRPMPICHD RFSDYMSINR PFFSKWGALG ELRTTSGMRR FGMLEIKEYD ATTKPGQLNV LLESDFEFVL TQSFSVLSRH AAKDYLQRHQ RNLIDARDVA TSQIEQIDEA LNQLISGHFV MGEHHCTLTI YGNTIQQVRD HMAKASAALL DVAVLPKPVD LAIEAGYWAQ LPGNWKWRPR PAPITSLNFL SFSSFHNFMS GKPTGNPWGP AVTILKTTSK TPLYFNFHAS KLEEDSTDKR LLGNTALIGQ SGSGKTVLLG FLVAQAQKFK PTTVVFDKDR GMEIAIRAMG GKYLTFKPGR KSGFNPFQLP PTQENLIFLK QFVKKLAEAG GEVTHHDEEE INKAVMSVMS SNIDQSLRRL SFLIQFLPNP RLSDYNAHPS VHARLLKWCE GGDYGWLFDN PHDALDLSTH QIYGFDITEF LDNFETRTPV MMYLLYRTEG MISGQRFMYI FDEFWKPLQD PYFEDLAKNK QKTIRKQNGI FVYATQEPSD ALESNIAKTL IQQCATYIFL ANPKANYEDY TKGFKLTDTE FELVKGLGEF SRQFLIKQGD QSALAELNLG KFHLTSGGKT IEHDFSDELL VLSGTPDNAE LAESIIAEVG DDPAIWLPIF LQHAKNDRRE T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag ctgttgaaag tagcaaacgc cttgcatcag agacacctgt aagcctgttt ctgccttatt cacatcatat tacagatacg attatctcta ctaaaaatgc ggaatattta tcgatttgga agattgacgg gcgttcacat caaagcgctt cagaagaaga tatttttcaa tggataaaag agcttaacaa tactctacgg ggtattgcat cagccaattt atcattttgg acacatattg tgcgtcgccg tgtttatgaa tatcctgatt caacatttga tcaaatgttt tgctatcaac ttgatgaaaa atatcggcaa agctttactg gttataattt gatggtcaat gatttatatc taactgtcat ttttcaaccg atagcggata aaattatgtc gttcttttct aaacatgaac gtgaaacgct tgatcaaaaa aagatgcgac aagactcatg tattaaagaa ctcaatgaca tcaatcgtac tttaggccaa tctttaaacc gttacggtgc agaacttctt ggtgcttatg aaaaaaatgg gcatgcttat tcttctgcac ttgagttcct tggaatgctt gtcaatggtg aatatcgccc catgccaatt tgccatgacc gtttttctga ctatatgtct atcaatcggc cttttttctc aaaatggggt gctcttggtg aattgcgaac aaccagtgga atgcgtcgat ttggaatgct ggaaattaaa gaatatgatg caacaaccaa accaggacaa ttaaatgttt tgttggaaag tgacttcgag ttcgtattga cgcagagctt ttcggtgctc tcccgacatg ctgctaaaga ctatttacaa cgacatcagc gtaaccttat tgatgcacga gatgtggcaa cgagccaaat tgaacaaatt gacgaagcac taaaccagct tattagcggg cattttgtca tgggagaaca tcactgcaca ttgactatct atggtaatac aattcaacaa gttcgcgacc atatggctaa agcaagtgca gcattactag acgttgcggt actgcccaaa cctgtagact tagcgataga ggctggctat tgggcacaac ttcctggcaa ttggaaatgg agaccacgtc ctgccccgat tacatcattg aactttttgt cattttcatc ttttcataat tttatgtcag gaaaacccac tggcaaccca tggggtccag ccgtcacaat tcttaaaaca accagtaaaa caccgcttta tttcaacttt catgcctcca aacttgaaga ggactcaaca gataaacgtt tgctcggtaa taccgcactt atcggacaat ctggttccgg taaaaccgta ttactcggct ttttggtagc acaagcacaa aaatttaagc cgacaacggt tgtttttgat aaagatcgtg gtatggaaat tgccattcga gcgatgggtg gaaaatattt aacattcaag ccaggcagga aaagtggttt caatcctttc caactcccac ccacacaaga aaatctgatt tttctgaaac aatttgttaa aaaattagca gaagctggtg gtgaggtcac gcatcacgac gaggaagaaa ttaacaaagc ggttatgtct gttatgagca gtaacattga ccaatcactc cgtcgtttgt cctttctgat acaattttta ccaaatccac gtttaagtga ttataatgct cacccatctg ttcatgctcg cttactaaaa tggtgtgaag gtggtgatta tggatggtta tttgataatc cccatgatgc tcttgatctc tcaacacatc aaatttacgg ctttgatatc acggaatttt tagataattt tgaaactcgc accccagtga tgatgtattt gctttaccgc acagaaggca tgattagcgg acagcgattt atgtatatct ttgatgagtt ttggaagcct ttgcaagatc cgtactttga agatttggcg aaaaataagc aaaagactat ccgtaaacaa aatggtattt ttgtttacgc aacacaagag cctagcgatg ccttggaaag taatattgcc aaaacactca ttcaacagtg tgcgacttac atttttctag ccaaccccaa ggcaaactat gaagattata caaaaggctt taaactaaca gatacagaat ttgaactcgt taaaggactt ggtgagttta gtcgccaatt cctcattaag cagggtgatc aatctgcgct tgcagaactg aatcttggta aattccatct gacaagcggt ggaaaaacca tcgaacacga ctttagtgac gagctcttgg tgttatcagg tacaccagat aacgcagaat tagccgaaag cattatcgcg gaagttggtg atgatccggc aatatggtta ccaattttct tacagcacgc aaaaaatgac aggagggaaa catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat