BagrA.18407.b

TrwE (Trw type IV secretion machinery)

CENTER ID: BagrA.18407.b
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.18407.b.B2.GE35927 soluble domain 86 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q0WXF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAGFQWYTK HNRDSIFGGL MFDNKEGEGK GTKLDNVEQK HIEGTYGGSE LGSERRPTIP GTRALLVVGL IVIVTIPIAL TWKAFKMRNT VEVEEENPQQ IVQQIIPNYV PRIIEEPKTV EEAEKPAQIE DSNLDIPPAL SKLLQTAIPP HMLQDSEELA RKRMLSSSLN NGGSEGSTDT NNKASNKDRG SGVLFDNLQP VRLGQSHAVQ LRNRDFLITQ GTQIDCTLET KIITSQPGMT TCHLTRDIYS TSGRVVLLDR GSKVVGFYQS GIQQGQTRVF VQWSRIETPS GVVVNLDSPG TGPLGEAGIG GWVDRRFWER FGSAIMVSII GDLGEWVKGK VSKISKENKE NSQNKGAQNA ESIAIDVLQN SINITPTLYK NQGERVNIFV ARDLDFSDVY SLVTR
NT Sequence
atggccgcgg gattccaatg gtataccaaa cacaacaggg acagtatctt cggtggttta atgtttgaca ataaggaagg agagggaaaa ggtacaaaac tggacaacgt cgagcaaaaa catattgaag gtacttatgg agggtcggaa ttaggttctg aacgtcgtcc gacaattcca ggtacccgtg cattgttagt ggtaggactt attgtcatag taacgatacc gattgccttg acttggaaag cttttaaaat gcgtaatacc gtggaagttg aggaagaaaa cccacaacaa atagtacagc aaattatacc aaactacgtc cctcggatta tagaagagcc aaagactgta gaagaagcag aaaaaccagc acaaatagaa gattcaaacc tagatattcc accggccttg agtaagcttc ttcaaacagc cattcctcca cacatgctcc aagattctga agaattagca cgcaaacgta tgctcagttc tagccttaac aacggtggta gcgaaggatc tacagataca aataataaag catcgaataa agatagaggt agtggcgtat tatttgataa ccttcagccc gtgcgtttag gtcaatcaca tgcggtgcaa cttcgtaacc gtgacttttt gattacgcaa ggaacgcaaa tagattgcac gctagaaaca aagatcatta catcacaacc cggaatgacg acatgtcatt taacacgcga tatttattcc acaagtggtc gtgttgtttt gcttgaccgt ggttctaaag tcgttggttt ttaccaaagt ggcatacaac aaggacaaac gcgtgtgttt gtgcaatggt cacgcatcga aacgccttct ggtgttgttg tcaatcttga ctcacctggc acaggtcctt tgggtgaagc gggtattggt ggctgggttg acagacgctt ttgggagaga tttggtagtg caattatggt aagcattatc ggtgatttag gagaatgggt aaaaggaaaa gttagtaaaa tcagtaaaga aaataaagag aactcacaaa ataaaggtgc tcagaatgct gaatctatag cgatcgatgt tcttcaaaac tccatcaata tcacaccaac actttacaaa aaccaaggcg aacgagtaaa tatttttgtt gctcgtgatc tggatttcag cgatgtctac agtcttgtca cacgttaa
Details for BagrA.18407.b.B2.GE35927
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 69
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMK MRNTVEVEEE NPQQIVQQII PNYVPRIIEE PKTVEEAEKP AQIEDSNLDI PPALSKLLQT AIPPHMLQDS EELARKRMLS SSLNNGGSEG STDTNNKASN KDRGSGVLFD NLQPVRLGQS HAVQLRNRDF LITQGTXIDC TLETKIITSQ PGMTTCHLTR DIYSTSGRVV LLDRGSKVVG FYQSGIQXXX TRVFVQWXRI EXXXXXXQXX XXXXXXGXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
tttntttaac tttaagaagg aganatacca tggctcacca ccaccaccac catatgaaaa tgcgtaatac cgtggaagtt gaggaagaaa acccacaaca aatagtacag caaattatac caaactacgt ccctcggatt atagaagagc caaagactgt agaagaagca gaaaaaccag cacaaataga agattcaaac ctagatattc caccggcctt gagtaagctt cttcaaacag ccattcctcc acacatgctc caagattctg aagaattagc acgcaaacgt atgctcagtt ctagccttaa caacggtggt agcgaaggat ctacagatac aaataataaa gcatcgaata aagatagagg tagtggcgta ttatttgata accttcagcc cgtgcgttta ggtcaatcac atgcggtgca acttcgtaac cgtgactttt tgattacgca aggaacgcan atagattgca cgctagaaac aaagatcatt acatcacaac ccggaatgac gacatgtcat ttaacacgcg atatttattc cacaagtggt cgtgttgttt tgcttgaccg tggntctaaa gtcgttggtt tttaccaaag tggnatacaa cangnanaaa cgcgtgtgtt tgtgcaatgg ncacgcatcg aancnnnncn gntgtntngt caatnntnnn nnnnnngnnn nntcntnggn tnnancnggn nnnnnnnnnt ggnnnnnnnn nnnttnngnn nnnattnnnn nnantngnna nnnntnnnnn gannnnnnna angnnnannn nnnnnnnnan nnnnnnnnna anncnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncnnnnnn nnnannnnnn nnnnnnannn nnnnnnnnnn nncnnnnnnn nnnannnnnc nnnnag
Expected Protein Sequence
MAHHHHHHMK MRNTVEVEEE NPQQIVQQII PNYVPRIIEE PKTVEEAEKP AQIEDSNLDI PPALSKLLQT AIPPHMLQDS EELARKRMLS SSLNNGGSEG STDTNNKASN KDRGSGVLFD NLQPVRLGQS HAVQLRNRDF LITQGTQIDC TLETKIITSQ PGMTTCHLTR DIYSTSGRVV LLDRGSKVVG FYQSGIQQGQ TRVFVQWSRI ETPSGVVVNL DSPGTGPLGE AGIGGWVDRR FWERFGSAIM VSIIGDLGEW VKGKVSKISK ENKENSQNKG AQNAESIAID VLQNSINITP TLYKNQGERV NIFVARDLDF SDVYSLVTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tgcgtaatac cgtggaagtt gaggaagaaa acccacaaca aatagtacag caaattatac caaactacgt ccctcggatt atagaagagc caaagactgt agaagaagca gaaaaaccag cacaaataga agattcaaac ctagatattc caccggcctt gagtaagctt cttcaaacag ccattcctcc acacatgctc caagattctg aagaattagc acgcaaacgt atgctcagtt ctagccttaa caacggtggt agcgaaggat ctacagatac aaataataaa gcatcgaata aagatagagg tagtggcgta ttatttgata accttcagcc cgtgcgttta ggtcaatcac atgcggtgca acttcgtaac cgtgactttt tgattacgca aggaacgcaa atagattgca cgctagaaac aaagatcatt acatcacaac ccggaatgac gacatgtcat ttaacacgcg atatttattc cacaagtggt cgtgttgttt tgcttgaccg tggttctaaa gtcgttggtt tttaccaaag tggcatacaa caaggacaaa cgcgtgtgtt tgtgcaatgg tcacgcatcg aaacgccttc tggtgttgtt gtcaatcttg actcacctgg cacaggtcct ttgggtgaag cgggtattgg tggctgggtt gacagacgct tttgggagag atttggtagt gcaattatgg taagcattat cggtgattta ggagaatggg taaaaggaaa agttagtaaa atcagtaaag aaaataaaga gaactcacaa aataaaggtg ctcagaatgc tgaatctata gcgatcgatg ttcttcaaaa ctccatcaat atcacaccaa cactttacaa aaaccaaggc gaacgagtaa atatttttgt tgctcgtgat ctggatttca gcgatgtcta cagtcttgtc acacgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t