BagrA.18404.b

Targeted effector protein YopP1 (Yop-like family protein)

CENTER ID: BagrA.18404.b
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.18404.b.B1.GE35631 full length 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|634504.3.peg.1906
RefSeq: YP_002972577.1
UniProt: C6AAE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPQDSSPPH SSQIPESTSA PEEASAQKAA KTPEGARANA SLDNLASHLE RLNIEKIRNT PFNRAELKDI VAGLEQHNAD GSWIKNYYDI LDLYMMPALV DQANRKYPEM NLKLATPPDG FALALKETLE NGIKSSRLIV NAHDRGIHFA VVDHQTIEDK TSLIFLEPTS LRDTLPALLA LRTRQSIEDH QLPNIHFAMA EMDIQRSSSE CGMFSLSLAK KLYLEADKLK RLHKDNIKGM LCEPDTPLPA EKLDPYLPAR LYKHVQGRRR LREYLKANPE AAHEKVNKKG ETLTERFERS LSVTETKTVS VSQHRKRATE YKSLMM
NT Sequence
atgaagccac aagattcttc cccccctcat tcctcacaaa tacccgaaag tacaagcgca cctgaagaag caagcgcaca aaaagctgcg aagacacccg aaggagcgcg tgcaaatgca tcactagaca atcttgcttc acatttagaa cggctcaaca tcgaaaaaat tagaaatact ccttttaatc gtgcggaatt aaaagatatt gttgctggtt tagaacagca taatgccgat ggaagttgga taaaaaatta ttatgatatt ttagatcttt acatgatgcc agccttggta gatcaagcaa atcgtaaata tccagagatg aatcttaaac ttgcgacgcc ccctgacggc tttgcccttg cgcttaaaga aacgctcgaa aacggcataa aatcttccag gctgattgtt aatgcgcatg ataggggaat ccattttgcg gtcgttgatc atcaaactat agaagacaaa acatctttga tctttttaga acctacctca ctgcgtgaca cgctccccgc cttacttgca ttaagaacgc gacaatccat tgaagatcac caattgccta acattcattt tgccatggca gaaatggata ttcaacgcag ttcctctgaa tgtgggatgt tcagcttaag ccttgccaaa aagctttatc tcgaagctga taaattaaaa agactgcaca aagataatat taaaggcatg ttatgtgaac cagatacgcc tttacctgct gaaaagttag atccatatct tccagctcgt ttatacaaac acgtgcaagg aagaagacgt cttagggaat atctaaaagc caatccagaa gcagcacatg aaaaagtaaa caaaaaagga gaaactctaa ccgagaggtt tgaaagaagc ttaagtgtaa cagagacaaa aactgtttct gtttcacaac acagaaaaag agcgacggaa tacaaatctc tgatgatgta a
Details for BagrA.18404.b.B1.GE35631
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMK PQDSSPPHSS QIPESTSAPE EASAQKAAKT PEGARANASL DNLASHLERL NIEKIRNTPF NRAELKDIVA GLEQHNADGS WIKNYYDILD LYMMPALVDQ ANRKYPEMNL KLATPPDGFA LALKETLENG IKSSRLIVNA HDRGIHFAVV DHQTIEDKTS LIFLEPTSLR DTLPALLALR TRQSIEDHQL PNIHFAMAEM DIQRSSSECG MFSLSLAKKL YLEADKLKRL HKDNIKGMLC EPDTPLPAEK LDPYLPARLY KHVQGRRRLX EYLKANPEAX HEKVNKKGEL
Validated NT Sequence
ttantttaag aaggaganat accatggctc accaccacca ccaccatatg aagccacaag attcttcccc ccctcattcc tcacaaatac ccgaaagtac aagcgcacct gaagaagcaa gcgcacaaaa agctgcgaag acacccgaag gagcgcgtgc aaatgcatca ctagacaatc ttgcttcaca tttagaacgg ctcaacatcg aaaaaattag aaatactcct tttaatcgtg cggaattaaa agatattgtt gctggtttag aacagcataa tgccgatgga agttggataa aaaattatta tgatatttta gatctttata tgatgccagc cttggtagat caagcaaatc gtaaatatcc agagatgaat cttaaacttg cgacgccccc tgacggcttt gcccttgcgc ttaaagaaac gctcgaaaac ggcataaaat cttccaggct gattgttaat gcgcatgata ggggaatcca ttttgcggtc gttgatcatc aaactataga agacaaaaca tctttgatct ttttagaacc tacctcactg cgtgacacgc tccccgcctt acttgcatta agaacgcgac aatccattga agatcaccaa ttgcctaaca ttcattttgc catggcagaa atggatattc aacgcagttc ctctgaatgt gggatgttca gcttaagcct tgccaaaaag ctttatctcg aagctgataa attaaaaaga ctgcacaaag ataatattaa aggcatgtta tgtgaaccag atacgccttt acctgctgaa aagttagatc catatcttcc agctcgttta tacaaacacg tgcaaggaag aagacgtctt anggaatatc taaaagccaa tccagaagca ncacatgaaa aagtaaacaa aaaaggagaa ctctaaccga naggtttgaa agaagcttnn gtgnaacnna nannaaactg tttctgnttc ncagcacaga aaaagagcga ngnnnacnaa tctctnanna ngnnannnan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaann aaaaanaaaa aaaaaaaaaa aa
Expected Protein Sequence
MAHHHHHHMK PQDSSPPHSS QIPESTSAPE EASAQKAAKT PEGARANASL DNLASHLERL NIEKIRNTPF NRAELKDIVA GLEQHNADGS WIKNYYDILD LYMMPALVDQ ANRKYPEMNL KLATPPDGFA LALKETLENG IKSSRLIVNA HDRGIHFAVV DHQTIEDKTS LIFLEPTSLR DTLPALLALR TRQSIEDHQL PNIHFAMAEM DIQRSSSECG MFSLSLAKKL YLEADKLKRL HKDNIKGMLC EPDTPLPAEK LDPYLPARLY KHVQGRRRLR EYLKANPEAA HEKVNKKGET LTERFERSLS VTETKTVSVS QHRKRATEYK SLMM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc cacaagattc ttccccccct cattcctcac aaatacccga aagtacaagc gcacctgaag aagcaagcgc acaaaaagct gcgaagacac ccgaaggagc gcgtgcaaat gcatcactag acaatcttgc ttcacattta gaacggctca acatcgaaaa aattagaaat actcctttta atcgtgcgga attaaaagat attgttgctg gtttagaaca gcataatgcc gatggaagtt ggataaaaaa ttattatgat attttagatc tttacatgat gccagccttg gtagatcaag caaatcgtaa atatccagag atgaatctta aacttgcgac gccccctgac ggctttgccc ttgcgcttaa agaaacgctc gaaaacggca taaaatcttc caggctgatt gttaatgcgc atgatagggg aatccatttt gcggtcgttg atcatcaaac tatagaagac aaaacatctt tgatcttttt agaacctacc tcactgcgtg acacgctccc cgccttactt gcattaagaa cgcgacaatc cattgaagat caccaattgc ctaacattca ttttgccatg gcagaaatgg atattcaacg cagttcctct gaatgtggga tgttcagctt aagccttgcc aaaaagcttt atctcgaagc tgataaatta aaaagactgc acaaagataa tattaaaggc atgttatgtg aaccagatac gcctttacct gctgaaaagt tagatccata tcttccagct cgtttataca aacacgtgca aggaagaaga cgtcttaggg aatatctaaa agccaatcca gaagcagcac atgaaaaagt aaacaaaaaa ggagaaactc taaccgagag gtttgaaaga agcttaagtg taacagagac aaaaactgtt tctgtttcac aacacagaaa aagagcgacg gaatacaaat ctctgatgat gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat