SSGCID: Target Page

BagrA.18404.a

Targeted effector protein YopP2 (Yop-like family protein)

CENTER ID:	BagrA.18404.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.18404.a.B1.GE35630	full length		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.18404.a.B1.PS01646	full length		1	309
BagrA.18404.a.B1.PS01647	full length		1	309

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.1908
RefSeq:	YP_002972578.1
UniProt:	C6AAE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPQDSKDTA AHASQIAESN IENESLESLI ARLEQLNAPQ KEESTLSQEK LKAIITDLED DLVTGRWINS YYENIDIRLM PALVEKANSK YPEMNLKLAL TAEEFSHALK EVVESDAKSA QFIVNSGSKI HFAVLDYQKV DDKISLIMLE PTTFQNITAC KLGIKINQTL QCLQLPPYSF TTAEMDIQRS SSECGMFSLS LAKKLHLESD KLARLHKDNV KGVLCEPNSS LSAEKLDSYL PISLYKHAQG RKRLEQYLKT NPDAEHETVN KKGETLRERF EKNLRTTEGK TVSVSSHQKR LTEYKSLMM

NT Sequence

atgaagccac aagattcaaa agatactgcc gctcatgcct cacaaatagc agaaagtaac atcgaaaatg aatccttaga aagccttatt gcccgtttag aacaactcaa cgctcctcaa aaagaagaga gcaccttgag tcaggaaaag ttgaaagcta ttattacaga cttagaggat gatctcgtca ccggccgttg gattaactct tattatgaaa atatagatat tagattgatg ccggctctcg tagaaaaagc aaacagcaaa tatccagaaa tgaatctcaa attggctctt acagcagaag agttttcgca cgcactaaag gaagtggttg agagcgacgc aaaatctgct caatttatag taaattcggg aagcaaaatc cactttgcag tccttgatta tcaaaaagtt gatgacaaaa tctctttgat tatgcttgaa cctacaacat ttcagaatat cactgcttgc aaactaggga taaaaataaa tcaaaccctt caatgtcttc aactgcctcc ttattctttt accacggcag aaatggatat tcagcgaagc tcttctgaat gtgggatgtt tagcttaagc ctcgccaaaa agcttcatct tgaatctgat aaattagcaa gattgcacaa agataatgtt aagggtgtat tatgtgaacc aaattcatct ttatctgctg aaaagttaga ttcctatctt cccatcagtt tatacaagca tgcgcaagga agaaaacgtc tagaacaata tttaaaaacc aatccggatg ctgaacatga aacagtaaac aaaaaaggtg aaactctgag agagagattt gaaaaaaact taagaacaac agaaggaaaa actgtttccg tttcctccca ccaaaaaagg ctcactgaat acaaatcttt gatgatgtaa

Details for BagrA.18404.a.B1.GE35630

HARVESTED ON:	7/10/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK PQDSKDTAAH ASQIAESNIE NESLESLIAR LEQLNAPQKE ESTLSQEKLK AIITDLEDDL VTGRWINSYY ENIDIRLMPA LVEKANSKYP EMNLKLALTA EEFSHALKEV VESDAKSARF IVNSGSKIHF AVLDYQKVDD KISLIMLEPT TFQNITACKL GIKINQTLQC LQLPPYSFTT AEMDIQRSSS ECGMFSLSLA KKLHLESDKL ARLHKDNVKG VLCEPNSSLS AEKLDSYLPI SLYKHAQGRK RLEQYLKTNP DAEHETVNKK GETLRERFEK NLRTTEGKTV SVSSHQKRLT EYKSLMM

Validated NT Sequence

tttaagaagg aganatacca tggctcacca ccaccaccac catatgaagc cacaagattc aaaagatact gccgctcatg cctcacaaat agcagaaagt aacatcgaaa atgaatcctt agaaagcctt attgcccgtt tagaacaact caacgctcct caaaaagaag agagcacctt gagtcaggaa aagttgaaag ctattattac agacttagag gatgatctcg tcaccggccg ttggattaac tcttattatg aaaatataga tattagattg atgccggctc tcgtagaaaa agcaaacagc aaatatccag aaatgaatct caaattggct cttacagcag aagagttttc gcacgcacta aaggaagtgg ttgagagcga cgcaaaatct gctcgattta tagtaaattc gggaagtaaa atccactttg cagtccttga ttatcaaaaa gttgatgaca aaatctcttt gattatgctt gaacctacaa catttcagaa tatcactgct tgcaaactag ggataaaaat aaatcaaacc cttcaatgtc ttcaactgcc tccttattct tttaccacgg cagaaatgga tattcagcga agctcttctg aatgtgggat gtttagctta agcctcgcca aaaagcttca tcttgaatct gataaattag caagattgca caaagataat gttaagggtg tattatgtga acccaattca tctttatctg ctgaaaagtt agattcctat cttcccatca gtttatacaa gcatgcgcaa ggaagaaaac gtctagaaca atatttaaaa accaatccgg atgctgaaca tgaaacagta aacaaaaaag gtgaaactct gagagagaga tttgaaaaaa acttaagaac aacagaagga aaaactgttt ccgtttcctc ccaccaaaaa aggctcactg aatacaaatc tttgatgatg taagtgagta

Expected Protein Sequence

MAHHHHHHMK PQDSKDTAAH ASQIAESNIE NESLESLIAR LEQLNAPQKE ESTLSQEKLK AIITDLEDDL VTGRWINSYY ENIDIRLMPA LVEKANSKYP EMNLKLALTA EEFSHALKEV VESDAKSAQF IVNSGSKIHF AVLDYQKVDD KISLIMLEPT TFQNITACKL GIKINQTLQC LQLPPYSFTT AEMDIQRSSS ECGMFSLSLA KKLHLESDKL ARLHKDNVKG VLCEPNSSLS AEKLDSYLPI SLYKHAQGRK RLEQYLKTNP DAEHETVNKK GETLRERFEK NLRTTEGKTV SVSSHQKRLT EYKSLMM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc cacaagattc aaaagatact gccgctcatg cctcacaaat agcagaaagt aacatcgaaa atgaatcctt agaaagcctt attgcccgtt tagaacaact caacgctcct caaaaagaag agagcacctt gagtcaggaa aagttgaaag ctattattac agacttagag gatgatctcg tcaccggccg ttggattaac tcttattatg aaaatataga tattagattg atgccggctc tcgtagaaaa agcaaacagc aaatatccag aaatgaatct caaattggct cttacagcag aagagttttc gcacgcacta aaggaagtgg ttgagagcga cgcaaaatct gctcaattta tagtaaattc gggaagcaaa atccactttg cagtccttga ttatcaaaaa gttgatgaca aaatctcttt gattatgctt gaacctacaa catttcagaa tatcactgct tgcaaactag ggataaaaat aaatcaaacc cttcaatgtc ttcaactgcc tccttattct tttaccacgg cagaaatgga tattcagcga agctcttctg aatgtgggat gtttagctta agcctcgcca aaaagcttca tcttgaatct gataaattag caagattgca caaagataat gttaagggtg tattatgtga accaaattca tctttatctg ctgaaaagtt agattcctat cttcccatca gtttatacaa gcatgcgcaa ggaagaaaac gtctagaaca atatttaaaa accaatccgg atgctgaaca tgaaacagta aacaaaaaag gtgaaactct gagagagaga tttgaaaaaa acttaagaac aacagaagga aaaactgttt ccgtttcctc ccaccaaaaa aggctcactg aatacaaatc tttgatgatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for BagrA.18404.a.B1.PS01646

PURIFICATION DATe:	10/3/2012
CONCENTRATION:	5.12mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC CACAAGATTC AAAAGATACT GCCGCTCATG CCTCACAAAT AGCAGAAAGT AACATCGAAA ATGAATCCTT AGAAAGCCTT ATTGCCCGTT TAGAACAACT CAACGCTCCT CAAAAAGAAG AGAGCACCTT GAGTCAGGAA AAGTTGAAAG CTATTATTAC AGACTTAGAG GATGATCTCG TCACCGGCCG TTGGATTAAC TCTTATTATG AAAATATAGA TATTAGATTG ATGCCGGCTC TCGTAGAAAA AGCAAACAGC AAATATCCAG AAATGAATCT CAAATTGGCT CTTACAGCAG AAGAGTTTTC GCACGCACTA AAGGAAGTGG TTGAGAGCGA CGCAAAATCT GCTCAATTTA TAGTAAATTC GGGAAGCAAA ATCCACTTTG CAGTCCTTGA TTATCAAAAA GTTGATGACA AAATCTCTTT GATTATGCTT GAACCTACAA CATTTCAGAA TATCACTGCT TGCAAACTAG GGATAAAAAT AAATCAAACC CTTCAATGTC TTCAACTGCC TCCTTATTCT TTTACCACGG CAGAAATGGA TATTCAGCGA AGCTCTTCTG AATGTGGGAT GTTTAGCTTA AGCCTCGCCA AAAAGCTTCA TCTTGAATCT GATAAATTAG CAAGATTGCA CAAAGATAAT GTTAAGGGTG TATTATGTGA ACCAAATTCA TCTTTATCTG CTGAAAAGTT AGATTCCTAT CTTCCCATCA GTTTATACAA GCATGCGCAA GGAAGAAAAC GTCTAGAACA ATATTTAAAA ACCAATCCGG ATGCTGAACA TGAAACAGTA AACAAAAAAG GTGAAACTCT GAGAGAGAGA TTTGAAAAAA ACTTAAGAAC AACAGAAGGA AAAACTGTTT CCGTTTCCTC CCACCAAAAA AGGCTCACTG AATACAAATC TTTGATGATG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT

Details for BagrA.18404.a.B1.PS01647

PURIFICATION DATe:	10/3/2012
CONCENTRATION:	15.68mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)