SSGCID: Target Page

BagrA.17330.c

BepF protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BagrA.17330.c
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.17330.c.B1.GE35690	full length		1	608
BagrA.17330.c.B2.GE37024	BID1		273	419

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.1564
RefSeq:	YP_002972375.1
UniProt:	C6AET1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKRNPSPFI TEMIEKFQQQ TEQSPTRTPP PQVSQSLPKP TVQKHPENLS HHPKVAPPPP PRMKNPVQTS TIPPSTKNQE TPPPQKKVAP PKPPRAKERQ QHKPTAQEFP SLQTKVAPPP PPRMREKTQT GTTIPNSKVP SSKIQENSSP HTKAAPQRPP REKDRQQNKT TAQEFPSLQT KVAPPPPPRM REKTQTGTTI PSSKIPSSKI QENSSPHTKA APQRPPREKD RQQSSSSSQD SGTYAVPPSR KPHRMENRKQ SDAVIPKPAS PHTAIEQPKP SHGQKNSINT MERELLLVAY QEEIQFLCEK VYGNKLILEE RIEAIKENSD MGEQLLWDIT ERPKSISKLA GRKVLGIKNH ARRKAEENLP TLRTAINDYV YTSKYTKSEK ISQASHTEQQ HQRQAQEEEL INQHLQNPLN SEKKIEPLSN QEIARRVQQD PSVRYGQREI EYWCEIVYND PFILQYRIED MQKIPEIGEE LVFQIENPSR SFAPLAGKQI LGIKNGARKA AEENLSTLCT VIKDYAETVK QLRETIVQNH QEEQQKRHQP LNDPDKKLQK QQNLSQPPKL PERSTAKQHQ EVVETSKQED QPHVRPRRAE TSKAIALS

NT Sequence

atgaaaaaaa gaaatccttc cccttttatc accgaaatga tagaaaaatt tcaacaacag actgaacaat cccccaccag aaccccccct ccccaagttt ctcaaagttt gccaaaacca actgtacaaa aacacccaga aaatttatca caccacccaa aggttgctcc accgcccccc ccacgcatga aaaacccagt gcaaaccagc acaatacctc ctagcaccaa aaaccaagag acgcctcccc cacaaaaaaa agtggctccg ccaaaacctc cgcgtgcaaa agagagacaa caacataaac caacagctca agagtttccc tccctacaga caaaagtggc tccgccccct cctccacgca tgagagaaaa aacacaaacc ggcacaacaa tccccaactc aaaagtcccc agttcaaaaa tccaagaaaa ctcctctccg cacacaaaag cagcgccaca aagacctcca cgcgaaaaag ataggcaaca aaacaaaaca acagctcaag agttcccctc cctacagaca aaagtggctc cgccccctcc tccacgcatg agagaaaaaa cacaaaccgg cacaacaatc cccagttcaa aaatccccag ttcaaaaatc caagaaaact cctctccgca cacaaaagca gcaccacaaa gacctccacg cgaaaaagat aggcaacaaa gtagttcatc atcacaagat agtggaacat atgcagtacc tccttcacga aaaccacacc gtatggaaaa cagaaaacaa agcgatgctg tcatcccaaa acctgcctct ccacacacag caattgaaca gccaaaaccg tctcacggac aaaaaaattc tattaataca atggaaagag agctattgct cgtagcatat caagaagaaa tacaattttt gtgcgaaaag gtttatggca acaagctgat tttagaagaa agaatagaag caatcaaaga aaactccgat atgggagaac agctcttatg ggatattaca gaacggccta agtctatttc taagcttgct ggtagaaaag ttcttggcat aaaaaatcat gcgcgcagaa aagctgaaga aaatcttcct accctacgta ctgccattaa cgattatgtg tatacttcaa aatatacaaa aagtgaaaag atttcacaag cttctcacac agaacaacaa caccagcgac aagcacaaga ggaagagcta ataaatcaac atttacaaaa tccactcaat tcagaaaaga aaatagagcc tctctcaaat caggaaattg cccgcagagt tcaacaagat ccatcggttc gatatggtca aagagaaatc gaatattggt gcgaaattgt ttataatgat ccatttattt tgcaatatag aatagaagat atgcaaaaaa ttccagagat aggagaagag cttgtatttc aaattgaaaa cccttctaga tcttttgctc cgcttgctgg taagcaaatc cttggcataa aaaatggagc acgcaaagcc gctgaagaaa atctttctac cctgtgtacc gtcattaagg attatgctga aactgtaaaa cagttaagag aaaccattgt acaaaaccat caggaagaac aacaaaaacg tcatcaaccg cttaacgatc ccgataaaaa gttgcaaaag cagcaaaact tatcgcagcc cccaaagtta cctgaacgct caacagcaaa gcagcatcaa gaggttgtag aaacttctaa acaagaagac cagccacatg ttcgaccgcg cagagccgaa acatcaaaag caatagcttt gtcgtga

Details for BagrA.17330.c.B1.GE35690

HARVESTED ON:	7/10/2012
SEQUENCED ON:	2/22/2016
EXPECTED MW:	70kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	45

Validated AA Sequence

MGEQLLWDIT ERXKSISKLX GRKVLGIKNH ARRKAEEXLP TXRTAINDYV YTSKYTKSEK ISQASHTEQQ HQRQAQEEEX INQHLQNPLN SEKKIEPLSN QEIARRVQQD PSVRYGQREI EYWCEIVYND PFILQYRIED MQKIPEIGEE LVFQIENPSR SFAPLAGKQI LGIKNGARKA AEENLSTLCT VIKDYAETVK QLRETIVQNH QEEQQKRHQP LNDPDKKLQK QQNLSQPPKL PERSTAKQHQ EVVETSKQED QPHVRPRRAE TSKAIALS

Validated NT Sequence

atcttactca cttacgacaa agctattgct tttgatgttt cggctctgcg cggtcgaaca tgtggctggt cttcttgttt agaagtttct acaacctctt gatgctgctt tgctgttgag cgttcaggta actttggggg ctgcgataag ttttgctgct tttgcaactt tttatcggga tcgttaagcg gttgatgacg tttttgttgt tcttcctgat ggttttgtac aatggtttct cttaactgtt ttacagtttc agcataatcc ttaatgacgg tacacagggt agaaagattt tcttcagcgg ctttgcgtgc tccatttttt atgccaagga tttgcttacc agcaagcgga gcaaaagatc tagaagggtt ttcaatttga aatacaagct cttctcctat ctctggaatt ttttgcatat cttctattct atattgcaaa ataaatggat cattataaac aatttcgcac caatattcga tttctctttg accatatcga accgatggat cttgttgaac tctgcgggca atttcctgat ttgagagagg ctctattttc ttttctgaat tgagtggatt ttgtaaatgt tgatttatta nctcttcctc ttgtgcttgt cgctggtgtt gttgttctgt gtgagaagct tgtgaaatct tttcactttt tgtatatttt gaagtataca cataatcgtt aatggcagta cgtanggtag gaagantttc ttcagctttt ctgcgcgcat gattttttat gccaagaact tttctaccan caagcttaga aatagacttn ngccgttctg taatatccca taagagctgt tctcccatat cnganttttc tttgattgnn tctattcntt cttctaanat cagct

Expected Protein Sequence

MAHHHHHHMK KRNPSPFITE MIEKFQQQTE QSPTRTPPPQ VSQSLPKPTV QKHPENLSHH PKVAPPPPPR MKNPVQTSTI PPSTKNQETP PPQKKVAPPK PPRAKERQQH KPTAQEFPSL QTKVAPPPPP RMREKTQTGT TIPNSKVPSS KIQENSSPHT KAAPQRPPRE KDRQQNKTTA QEFPSLQTKV APPPPPRMRE KTQTGTTIPS SKIPSSKIQE NSSPHTKAAP QRPPREKDRQ QSSSSSQDSG TYAVPPSRKP HRMENRKQSD AVIPKPASPH TAIEQPKPSH GQKNSINTME RELLLVAYQE EIQFLCEKVY GNKLILEERI EAIKENSDMG EQLLWDITER PKSISKLAGR KVLGIKNHAR RKAEENLPTL RTAINDYVYT SKYTKSEKIS QASHTEQQHQ RQAQEEELIN QHLQNPLNSE KKIEPLSNQE IARRVQQDPS VRYGQREIEY WCEIVYNDPF ILQYRIEDMQ KIPEIGEELV FQIENPSRSF APLAGKQILG IKNGARKAAE ENLSTLCTVI KDYAETVKQL RETIVQNHQE EQQKRHQPLN DPDKKLQKQQ NLSQPPKLPE RSTAKQHQEV VETSKQEDQP HVRPRRAETS KAIALS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaagaaatcc ttcccctttt atcaccgaaa tgatagaaaa atttcaacaa cagactgaac aatcccccac cagaaccccc cctccccaag tttctcaaag tttgccaaaa ccaactgtac aaaaacaccc agaaaattta tcacaccacc caaaggttgc tccaccgccc cccccacgca tgaaaaaccc agtgcaaacc agcacaatac ctcctagcac caaaaaccaa gagacgcctc ccccacaaaa aaaagtggct ccgccaaaac ctccgcgtgc aaaagagaga caacaacata aaccaacagc tcaagagttt ccctccctac agacaaaagt ggctccgccc cctcctccac gcatgagaga aaaaacacaa accggcacaa caatccccaa ctcaaaagtc cccagttcaa aaatccaaga aaactcctct ccgcacacaa aagcagcgcc acaaagacct ccacgcgaaa aagataggca acaaaacaaa acaacagctc aagagttccc ctccctacag acaaaagtgg ctccgccccc tcctccacgc atgagagaaa aaacacaaac cggcacaaca atccccagtt caaaaatccc cagttcaaaa atccaagaaa actcctctcc gcacacaaaa gcagcaccac aaagacctcc acgcgaaaaa gataggcaac aaagtagttc atcatcacaa gatagtggaa catatgcagt acctccttca cgaaaaccac accgtatgga aaacagaaaa caaagcgatg ctgtcatccc aaaacctgcc tctccacaca cagcaattga acagccaaaa ccgtctcacg gacaaaaaaa ttctattaat acaatggaaa gagagctatt gctcgtagca tatcaagaag aaatacaatt tttgtgcgaa aaggtttatg gcaacaagct gattttagaa gaaagaatag aagcaatcaa agaaaactcc gatatgggag aacagctctt atgggatatt acagaacggc ctaagtctat ttctaagctt gctggtagaa aagttcttgg cataaaaaat catgcgcgca gaaaagctga agaaaatctt cctaccctac gtactgccat taacgattat gtgtatactt caaaatatac aaaaagtgaa aagatttcac aagcttctca cacagaacaa caacaccagc gacaagcaca agaggaagag ctaataaatc aacatttaca aaatccactc aattcagaaa agaaaataga gcctctctca aatcaggaaa ttgcccgcag agttcaacaa gatccatcgg ttcgatatgg tcaaagagaa atcgaatatt ggtgcgaaat tgtttataat gatccattta ttttgcaata tagaatagaa gatatgcaaa aaattccaga gataggagaa gagcttgtat ttcaaattga aaacccttct agatcttttg ctccgcttgc tggtaagcaa atccttggca taaaaaatgg agcacgcaaa gccgctgaag aaaatctttc taccctgtgt accgtcatta aggattatgc tgaaactgta aaacagttaa gagaaaccat tgtacaaaac catcaggaag aacaacaaaa acgtcatcaa ccgcttaacg atcccgataa aaagttgcaa aagcagcaaa acttatcgca gcccccaaag ttacctgaac gctcaacagc aaagcagcat caagaggttg tagaaacttc taaacaagaa gaccagccac atgttcgacc gcgcagagcc gaaacatcaa aagcaatagc tttgtcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BagrA.17330.c.B2.GE37024

HARVESTED ON:	6/12/2013
SEQUENCED ON:	7/9/2013
EXPECTED MW:	18kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHTA IEQPKPSHGQ KNSINTMERE LLLVAYQEEI QFLCEKVYGN KLILEERIEA IKENSDMGEQ LLWDITERPK SISKLAGRKV LGIKNHARRK AEENLPTLRT AINDYVYTSK YTKSEKISQA SHTEQQHQRQ AQEEELINQH LQNPL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacagcaa ttgaacagcc aaaaccgtct cacggacaaa aaaattctat taatacaatg gaaagagagc tattgctcgt agcatatcaa gaagaaatac aatttttgtg cgaaaaggtt tatggcaaca agctgatttt agaagaaaga atagaagcaa tcaaagaaaa ctccgatatg ggagaacagc tcttatggga tattacagaa cggcctaagt ctatttctaa gcttgctggt agaaaagttc ttggcataaa aaatcatgcg cgcagaaaag ctgaagaaaa tcttcctacc ctacgtactg ccattaacga ttatgtgtat acttcaaaat atacaaaaag tgaaaagatt tcacaagctt ctcacacaga acaacaacac cagcgacaag cacaagagga agagctaata aatcaacatt tacaaaatcc actctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat