BagrA.17330.b

BepE protein (VirB-translocated Bartonella effector protein)

CENTER ID: BagrA.17330.b
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.17330.b.B1.GE35689 full length 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|634504.3.peg.1561
RefSeq: YP_002972374.1
UniProt: C6AET0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKDHPHPFP SPSIQEIIAL YEKAAAEVTS PESLYKEAPS QSKRLSSIPE KDEERQSLLK EETVHASTNA TMPLTRGGST KRQQQPETTH TTVTSQVTQR AIRVLSEEEI ILLLPHHSLV RTYQEEIQRL SAKAYGNPDI LQEKMQEIQK NPAAGKELSW RMAAHPESIA KLSGTSTLGF KNRTRKQAEE DFSTLCVTVD CYTKAVKQAR ESLLQSPEQE LKNYERLMGK AAVTELLQKP DQREKETLSE AEISTKIQQH SKVKRHYAQI KYWCEVVFGN ANILQSQVET LFQSPEAIET LAQQLVGNPQ SFHKYAGISL CGFKNQARRH AEAGLSHLID AADNYATAVT QVRESILQTE QAKQKRHEPS SKQTQDLHQQ QRLSQSVGRT EHSTVTHEEA TTSTQQRETD IRLRKTVISK EMAFAN
NT Sequence
atgaaaaaag atcaccctca cccttttcca tccccttcaa tacaagaaat tatagcgctc tatgaaaaag cggcggcaga ggttacaagc ccagaaagcc tctataaaga agctccttca caaagcaaaa gactgtcatc cataccggaa aaagatgagg aaagacaatc tcttttaaaa gaagaaactg tacatgcatc aacaaatgca acaatgcctc tcacaagagg cggctctacc aaaaggcagc aacaacctga aacgacacac acaacagtta cttcacaagt tacccaaagg gcaataagag ttctctcaga ggaagaaatc attctattgc ttccacacca ttcattggtt cgaacctatc aagaagaaat tcagcgttta tctgcaaaag cctatggcaa ccccgacatt ttacaagaaa aaatgcagga aattcaaaaa aatcctgctg caggaaagga gctctcatgg cgcatggcag cccatcctga aagtattgct aaactttcag gcaccagcac gttgggcttc aaaaatcgaa cgcgcaaaca agcggaagaa gatttttcca ccctttgcgt caccgttgat tgttatacaa aagctgtaaa acaggcaaga gaaagcctat tgcaatctcc agagcaagaa ctcaaaaact atgaacgttt aatgggaaag gcagcagtca ctgaacttct gcaaaaacca gatcaacgtg aaaaagaaac tctttcagag gcagaaattt ctaccaaaat tcaacaacat tcaaaagtca aaaggcacta tgcccagatc aaatattggt gcgaagttgt ttttggtaac gcaaacattt tgcaatcaca agtggaaact cttttccaaa gccctgaagc aatagaaaca ctcgcacagc aacttgtcgg aaatcctcaa tcttttcaca agtacgctgg tatcagtctt tgtggcttta aaaatcaagc acgcagacat gctgaagctg gtctttctca cttaattgac gctgctgaca attatgcaac ggctgtaaca caagtaagag agagcatttt gcaaaccgaa caggcaaaac aaaagcgcca tgaaccatct tctaaacaga cacaagattt gcatcaacaa caacgcttat cacaatctgt tggacgcact gagcattcaa cagtaactca tgaagaagca acaacctcta ctcaacaaag agaaacagat attcgtcttc gcaaaactgt gatatcaaaa gaaatggctt ttgccaacta a
Details for BagrA.17330.b.B1.GE35689
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 74
Validated AA Sequence
MKKDHPHPFP SPSIQEIIAL YEKAAAEVTS PESLYKEAPS QSKRLSSIPE KDEERQSLLK EETVHASTNA TMPLTRGGST KRQQQPETTH TTVTSQVTQR AIRVLSEEEI ILLLPHHSLV RTYQEEIQRL SAKAYGNPDI LQEKMQEIQK NPAAGKELSW RMAAHPESIA KLSGTSTLGF KNRTRKQAEE DFSTLCVTVD CYTKAVKQAR ESLLQSPEQE LKNYERLMGK AAVTELLQKP DQREKETLSE AEISTKIQQH SKVKRHYAQI KYWCEVVFGN ANILQSQVET LFQSPXAIET LAQXLVXXXX XFXXYAGISL XXXX
Validated NT Sequence
ggctcaccac caccaccacc accatatgaa aaaagatcac cctcaccctt ttccatcccc ttcaatacaa gaaattatag cgctctatga aaaagcggcg gcagaggtta caagcccaga aagcctctat aaagaagctc cttcacaaag caaaagactg tcatccatac cggaaaaaga tgaggaaaga caatctcttt taaaagaaga aactgtacat gcatcaacaa atgcaacaat gcctctcaca agaggcggct ctaccaaaag gcagcaacaa cctgaaacga cacacacaac agttacttca caagttaccc aaagggcaat aagagttctc tcagaggaag aaatcattct attgcttcca caccattcat tggttcgaac ctatcaagaa gaaattcagc gtttatctgc aaaagcctat ggcaaccccg acattttaca agaaaaaatg caggaaattc aaaaaaatcc tgctgcagga aaggagctct catggcgcat ggcagcccat cctgaaagta ttgctaaact ttcaggcacc agcacgttgg gcttcaaaaa tcgaacgcgc aaacaagcgg aagaagattt ttccaccctt tgcgtcaccg ttgattgtta tacaaaagct gtaaaacagg caagagaaag cctattgcaa tctccagagc aagaactcaa aaactatgaa cgtttaatgg gaaaggcagc agtcactgaa cttctgcaaa aaccagatca acgtgaaaaa gaaactcttt cagaggcaga aatttctacc aaaattcaac aacattcaaa agtcaaaagg cactatgccc agatcaaata ttggtgcgaa gttgtttttg gtaacgcaaa cattttgcaa tcacaagtgg aaactctttt ccaaagccct gangcaatag aaacactcgc acagcnnctt gtcnnnnnnn nnnnnncntt tnnnnngtac gctggtatca gtctttnnnn cnnnnnnaa
Expected Protein Sequence
MAHHHHHHMK KDHPHPFPSP SIQEIIALYE KAAAEVTSPE SLYKEAPSQS KRLSSIPEKD EERQSLLKEE TVHASTNATM PLTRGGSTKR QQQPETTHTT VTSQVTQRAI RVLSEEEIIL LLPHHSLVRT YQEEIQRLSA KAYGNPDILQ EKMQEIQKNP AAGKELSWRM AAHPESIAKL SGTSTLGFKN RTRKQAEEDF STLCVTVDCY TKAVKQARES LLQSPEQELK NYERLMGKAA VTELLQKPDQ REKETLSEAE ISTKIQQHSK VKRHYAQIKY WCEVVFGNAN ILQSQVETLF QSPEAIETLA QQLVGNPQSF HKYAGISLCG FKNQARRHAE AGLSHLIDAA DNYATAVTQV RESILQTEQA KQKRHEPSSK QTQDLHQQQR LSQSVGRTEH STVTHEEATT STQQRETDIR LRKTVISKEM AFAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aagatcaccc tcaccctttt ccatcccctt caatacaaga aattatagcg ctctatgaaa aagcggcggc agaggttaca agcccagaaa gcctctataa agaagctcct tcacaaagca aaagactgtc atccataccg gaaaaagatg aggaaagaca atctctttta aaagaagaaa ctgtacatgc atcaacaaat gcaacaatgc ctctcacaag aggcggctct accaaaaggc agcaacaacc tgaaacgaca cacacaacag ttacttcaca agttacccaa agggcaataa gagttctctc agaggaagaa atcattctat tgcttccaca ccattcattg gttcgaacct atcaagaaga aattcagcgt ttatctgcaa aagcctatgg caaccccgac attttacaag aaaaaatgca ggaaattcaa aaaaatcctg ctgcaggaaa ggagctctca tggcgcatgg cagcccatcc tgaaagtatt gctaaacttt caggcaccag cacgttgggc ttcaaaaatc gaacgcgcaa acaagcggaa gaagattttt ccaccctttg cgtcaccgtt gattgttata caaaagctgt aaaacaggca agagaaagcc tattgcaatc tccagagcaa gaactcaaaa actatgaacg tttaatggga aaggcagcag tcactgaact tctgcaaaaa ccagatcaac gtgaaaaaga aactctttca gaggcagaaa tttctaccaa aattcaacaa cattcaaaag tcaaaaggca ctatgcccag atcaaatatt ggtgcgaagt tgtttttggt aacgcaaaca ttttgcaatc acaagtggaa actcttttcc aaagccctga agcaatagaa acactcgcac agcaacttgt cggaaatcct caatcttttc acaagtacgc tggtatcagt ctttgtggct ttaaaaatca agcacgcaga catgctgaag ctggtctttc tcacttaatt gacgctgctg acaattatgc aacggctgta acacaagtaa gagagagcat tttgcaaacc gaacaggcaa aacaaaagcg ccatgaacca tcttctaaac agacacaaga tttgcatcaa caacaacgct tatcacaatc tgttggacgc actgagcatt caacagtaac tcatgaagaa gcaacaacct ctactcaaca aagagaaaca gatattcgtc ttcgcaaaac tgtgatatca aaagaaatgg cttttgccaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat