SSGCID: Target Page

BagrA.17330.a

BepC protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BagrA.17330.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.17330.a.B1.GE35688	full length		1	532

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.1557
RefSeq:	YP_002972371.1
UniProt:	C6AES7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLEQNYLYKG TLTLKNKYGI KDPKKLYERC AHDTAREAVN FRHEPLPQKF DSTYLKLIHW SLFHKTFEWA GKTRDVSFTF EDGTSAHMPA MRPKGYEVPF AVGPQIQKKL KQLEKTLNEK NNLKGLSRQE FAENAAEVFM VLEHAHPFRK GNGRVNRMFM EKLGQAAGHP IDFSFITKGR MTAACIEAMQ YGNPQPMKDL FEDITHPQKS LVLKEFIFQM RNAGLDEINN RIVVAAKEGI PYEGIFRGFS AEGFVMEVEG AFVVGHKDDL PPELVKTLLN GAHLCFQKTN IQSLKETLIP KETLAPLTHE ELFTRISNDA YVEACRKEIE NLSKIVYGKA HALKTKMDIL TADPSLGNQF ADQILQNPQS ISKLAGRKIL DMKSPNRRQA EQTAPQLSQA LRNYTTITEQ TKEEILEQHQ REQKRLSHTV ETPEKNLQNL FALPREQQRE ALSHSRSLRQ ELHHFSRQLH NRLSSEDRKA IQEKDYTRLA CLLGTSESRA KEIANTVRNT KEAQCQTHTL KVNRSSSLAL TG

NT Sequence

atgttagagc aaaattattt atataaaggt accttaacac tcaaaaataa atatggcata aaagatccca aaaaactgta tgaacgctgt gcccatgata ccgccagaga agccgtcaat tttcgccatg aaccacttcc acaaaaattt gattccactt atttaaaatt aattcactgg agcctcttcc acaaaacttt tgaatgggct ggaaaaaccc gcgatgtatc ctttacattt gaagacggaa cctctgccca tatgccggcg atgcgtccca aaggttatga agttcctttt gctgtcggac cgcagattca aaaaaaacta aaacaactcg aaaaaacact taatgagaag aacaatttaa aaggtttatc gcgccaagaa tttgctgaaa atgccgctga agtttttatg gtgctcgagc acgcacatcc tttccgaaaa gggaatgggc gcgtaaatcg aatgtttatg gaaaaacttg gacaagcagc aggacatccc attgactttt ctttcatcac aaaaggacgc atgacagcag cttgtattga agccatgcaa tatggcaatc cccaaccgat gaaagatctt tttgaagata tcacgcatcc gcaaaaatcc cttgttttaa aagaattcat cttccaaatg agaaatgctg gactggatga aattaacaat cgtattgttg ttgccgcaaa agaagggatc ccctatgaag gcatctttag aggtttttca gcagaaggtt ttgtcatgga agtagaaggt gctttcgtcg tcggccacaa agatgatctc cccccagaac tcgtcaaaac attactcaac ggtgcccacc tctgctttca aaaaaccaac attcaaagct taaaagaaac actgatccca aaagaaacat tagcacctct cacacatgaa gagttattca caaggatttc aaatgacgct tatgttgaag catgcagaaa agaaatcgaa aatttatcaa agattgttta tggtaaagcc catgccttaa agaccaaaat ggacatctta acggcagatc caagcttagg aaatcagttt gccgatcaaa ttttacaaaa ccctcaatca atttctaaac ttgcaggaag aaaaatactt gacatgaaaa gtccaaatcg cagacaggcg gaacaaaccg ctccacaact cagccaagcc cttagaaatt ataccacaat aacagaacaa acaaaagaag aaattttaga gcaacatcaa agagaacaaa aacgcttgag ccacactgtc gaaacgcctg aaaaaaattt acaaaacctt tttgccttac caagagaaca acaaagagaa gctttatcgc attctcgttc actgcgacaa gaactccatc acttttcgcg ccaactacac aatcgtctat cttcagaaga tcgtaaagcc atacaagaaa aagattatac cagactagct tgcctacttg ggacatcgga aagcagagca aaagaaattg cgaatactgt aaggaacacc aaagaagcac aatgtcaaac tcacacctta aaagttaatc gctcttcatc acttgctctt accggctaa

Details for BagrA.17330.a.B1.GE35688

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	64

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgtta gagcaaaatt atttatataa aggtacctta acactcaaaa ataaatatgg cataaaagat cccaaaaaac tgtatgaacg ctgtgcccat gataccgcca gagaagccgt caattttcgc catgaaccac ttccacaaaa atttgattcc acttatttaa aattaattca ctggagcctc ttccacaaaa cttttgaatg ggctggaaaa acccgcgatg tatcctttac atttgaagac ggaacctctg cccatatgcc ggcgatgcgt cccaaaggtt atgaagttcc ttttgctgtc ggaccgcaga ttcaaaaaaa actaaaacaa ctcgaaaaaa cacttaatga gaagaacaat ttaaaaggtt tatcgcgcca agaatttgct gaaaatgccg ctgaagtttt tatggtgctc gagcacgcac atcctttccg aaaagggaat gggcgcgtaa atcgaatgtt tatggaaaaa cttggacaag cagcaggaca tcccattgac ttttctttca tcacaaaagg acgcatgaca gcagcttgta ttgaagccat gcaatatggc aatccccaac cgatgaaaga tctttttgaa gatatcacgc atccgcaaaa atcccttgtt ttaaaagaat tcatcttcca aatgagaaat gctggactgg atgaaattaa caatcgtatt gttgttgccg caaaagaagg gatcccctat gaaggcatct ttagaggttt ttcagcagaa ggttttgtca tggaagtaga aggtgctttc gtcgtcggcc acaaagatga tctcccccca gaactcgtca aaacattact caacggtgcc cacctctgct ttcaaaaaac caacattcaa agcttaaaag aaacactgat cccaaaagaa acattagcac ctctcacaca tgaagagtta ttcacaagga tttcaaatga cgcttatgtt gaagcatgca gaaaagaaat cgaaaattta tcaaagattg tttatggtaa agcccatgcc

Expected Protein Sequence

MAHHHHHHML EQNYLYKGTL TLKNKYGIKD PKKLYERCAH DTAREAVNFR HEPLPQKFDS TYLKLIHWSL FHKTFEWAGK TRDVSFTFED GTSAHMPAMR PKGYEVPFAV GPQIQKKLKQ LEKTLNEKNN LKGLSRQEFA ENAAEVFMVL EHAHPFRKGN GRVNRMFMEK LGQAAGHPID FSFITKGRMT AACIEAMQYG NPQPMKDLFE DITHPQKSLV LKEFIFQMRN AGLDEINNRI VVAAKEGIPY EGIFRGFSAE GFVMEVEGAF VVGHKDDLPP ELVKTLLNGA HLCFQKTNIQ SLKETLIPKE TLAPLTHEEL FTRISNDAYV EACRKEIENL SKIVYGKAHA LKTKMDILTA DPSLGNQFAD QILQNPQSIS KLAGRKILDM KSPNRRQAEQ TAPQLSQALR NYTTITEQTK EEILEQHQRE QKRLSHTVET PEKNLQNLFA LPREQQREAL SHSRSLRQEL HHFSRQLHNR LSSEDRKAIQ EKDYTRLACL LGTSESRAKE IANTVRNTKE AQCQTHTLKV NRSSSLALTG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag agcaaaatta tttatataaa ggtaccttaa cactcaaaaa taaatatggc ataaaagatc ccaaaaaact gtatgaacgc tgtgcccatg ataccgccag agaagccgtc aattttcgcc atgaaccact tccacaaaaa tttgattcca cttatttaaa attaattcac tggagcctct tccacaaaac ttttgaatgg gctggaaaaa cccgcgatgt atcctttaca tttgaagacg gaacctctgc ccatatgccg gcgatgcgtc ccaaaggtta tgaagttcct tttgctgtcg gaccgcagat tcaaaaaaaa ctaaaacaac tcgaaaaaac acttaatgag aagaacaatt taaaaggttt atcgcgccaa gaatttgctg aaaatgccgc tgaagttttt atggtgctcg agcacgcaca tcctttccga aaagggaatg ggcgcgtaaa tcgaatgttt atggaaaaac ttggacaagc agcaggacat cccattgact tttctttcat cacaaaagga cgcatgacag cagcttgtat tgaagccatg caatatggca atccccaacc gatgaaagat ctttttgaag atatcacgca tccgcaaaaa tcccttgttt taaaagaatt catcttccaa atgagaaatg ctggactgga tgaaattaac aatcgtattg ttgttgccgc aaaagaaggg atcccctatg aaggcatctt tagaggtttt tcagcagaag gttttgtcat ggaagtagaa ggtgctttcg tcgtcggcca caaagatgat ctccccccag aactcgtcaa aacattactc aacggtgccc acctctgctt tcaaaaaacc aacattcaaa gcttaaaaga aacactgatc ccaaaagaaa cattagcacc tctcacacat gaagagttat tcacaaggat ttcaaatgac gcttatgttg aagcatgcag aaaagaaatc gaaaatttat caaagattgt ttatggtaaa gcccatgcct taaagaccaa aatggacatc ttaacggcag atccaagctt aggaaatcag tttgccgatc aaattttaca aaaccctcaa tcaatttcta aacttgcagg aagaaaaata cttgacatga aaagtccaaa tcgcagacag gcggaacaaa ccgctccaca actcagccaa gcccttagaa attataccac aataacagaa caaacaaaag aagaaatttt agagcaacat caaagagaac aaaaacgctt gagccacact gtcgaaacgc ctgaaaaaaa tttacaaaac ctttttgcct taccaagaga acaacaaaga gaagctttat cgcattctcg ttcactgcga caagaactcc atcacttttc gcgccaacta cacaatcgtc tatcttcaga agatcgtaaa gccatacaag aaaaagatta taccagacta gcttgcctac ttgggacatc ggaaagcaga gcaaaagaaa ttgcgaatac tgtaaggaac accaaagaag cacaatgtca aactcacacc ttaaaagtta atcgctcttc atcacttgct cttaccggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat