SSGCID: Target Page

BagrA.01664.a

Two-component sensor histidine kinase BatS (BatR-BatS two-component system)

CENTER ID:	BagrA.01664.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.01664.a.B2.GE35924	kinase domain		333	594
BagrA.01664.a.B3.GE36582	periplasmic domain		68	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.62
RefSeq:	YP_002971124.1
UniProt:	C6AB80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDNSQLETL QKTTVNGTSS TPFLMFARLH LRTQRLFRQL LFSSLTRRIV ILNIAALAIL VTGILYLNQF RDGLIEAKIK SLCTQGKIIA GAIAASATVD TNSILIDPQK LLELQTGESV TPAPQSTDSW DFPINPEQVA PLLRRLITPK TTRARIYDRD ATLLLDSRVL YSSGEVFSYD LPPLKTEQSM WDRLTSWYSS TFYDKGIALD REQTKTSGIA YPEVYRALNG SLATAKRRNR QGQLIVSVAV PVQRYRAVVG ALLLSTTGSD IDNIVKGERL VIFKVFAVVG SVLLVLSLFL AHTIAHPLSK LSASANRVRN GNNQRVEIPD FSMREDEIGH LSTSIRDMTN ALYMRIETIE RFAADVSHEL KNPLTSLRSA VETLPLAKNE EAQEKLLEII QHDVRRLDRL ITDISDASRL DAELARETAE IVDMTPLLES LVHAVQEVYR NTQTIDISLN IVPRPHGKAY LVLGHELRLG QVISNLLENA RSFIPRNRGK ICITMKSHAS TLILTIEDNG PGIRSENIER IFERFYTDRP NEDAFGQNSG LGLSISRQII EAHNGTITAE NIIDSTLGKC KTGARFIIML PLAK

NT Sequence

atgacagaca attctcaact ggaaacttta caaaaaacaa ccgttaatgg tacatcctca acgccatttc ttatgtttgc ccgtttgcat ctccgaacac aacgtctctt tagacaattg cttttttcca gcctcacacg tcgcattgtt atattaaaca ttgctgccct tgccatttta gttacaggca ttttatacct taaccaattt cgcgatggtt taattgaagc aaaaattaaa agtttatgta cccaaggaaa aattattgct ggggctattg ctgcttccgc aacagtagat acaaattcta tcctcattga tccccaaaaa ctcttagagt tacagactgg tgaaagcgtt acacccgccc ctcaatcaac cgattcttgg gatttcccta ttaaccctga gcaagttgcc cctcttttac ggcgtctcat cacacctaaa acgacaagag cacgtatcta tgatcgtgat gctactctcc ttcttgattc gcgcgttctt tattctagtg gagaagtttt tagttatgat ttacccccac ttaaaacaga acaaagcatg tgggatcgcc ttacttcatg gtattcaagc acattttatg acaaaggtat tgctcttgac agagaacaaa caaaaaccag tggtattgct tatccagaag tgtaccgcgc attaaacgga tctcttgcta cggctaaaag acgcaacaga caaggtcaat taattgtttc tgttgccgtt cctgtgcaac gctatcgcgc agtggttgga gctcttcttc tttcaaccac tggttctgat attgataaca ttgtaaaagg tgaaagactg gttatcttta aagtttttgc cgttgtaggc agcgtacttt tggttctttc tctctttttg gcccatacga ttgcccatcc attaagtaaa ttatctgcct cggctaatcg agtgcgtaat ggcaataatc agcgtgtaga aattcctgat ttttcaatgc gtgaagatga aattggtcac ctttcaacct ctattcgtga tatgactaat gccctttata tgcgtattga aaccattgaa cgctttgctg ctgatgtaag ccacgaatta aaaaatcctc tcacctctct acgcagtgct gttgaaacgc tccctctggc taaaaatgaa gaagcacaag agaaattgct tgaaattatt caacatgatg tgcgccgtct tgatcggttg attacagata tttctgatgc atcgcggctc gatgcagagc ttgcacgaga aaccgcagag attgttgata tgacaccgct tttggaaagt cttgttcatg ctgttcagga agtgtatcgt aatacgcaaa ccattgatat aagccttaat attgtcccac gtcctcatgg aaaagcctat cttgtattgg gacatgaact ccgtttaggg caagtgattt caaatcttct tgaaaatgca cgttctttta ttcctcgtaa tcgtggcaaa atttgcatta ctatgaaaag tcatgcttca acccttattt taaccattga agataatggc ccaggtattc gttcagaaaa tattgaacgc atttttgagc gtttttatac agatcgacca aacgaagatg cttttggaca aaactctgga cttggacttt ctattagtcg gcaaatcatc gaagctcata atgggacaat tacagccgaa aatattattg attctacact tgggaagtgt aaaactggtg cacgttttat tattatgctt cccctcgcta aataa

Details for BagrA.01664.a.B2.GE35924

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR EDEIGHLSTS IRDMTNALYM RIETIERFAA DVSHELKNPL TSLRSAVETL PLAKNEEAQE KLLEIIQHDV RRLDRLITDI SDASRLDAEL ARETAEIVDM TPLLESLVHA VQEVYRNTQT IDISLNIVPR PHGKAYLVLG HELRLGQVIS NLLENARSFI PRNRGKICIT MKSHASTLIL TIEDNGPGIR SENIERIFER FYTDRPNEDA FGQNSGLGLS ISRQIIEAHN GTITAENIID STLGKCKTGA RFIIMLPLAK

Validated NT Sequence

tcttactcac ttatttagcg aggggaagca taataataaa acgtgcacca gttttacact tcccaagtgt agaatcaata atattttcgg ctgtaattgt cccattatga gcttcgatga tttgccgact aatagaaagt ccaagtccag agttttgtcc aaaagcatct tcgtttggtc gatctgtata aaaacgctca aaaatgcgtt caatattttc tgaacgaata cctgggccat tatcttcaat ggttaaaata agggttgaag catgactttt catagtaatg caaattttgc cacgattacg aggaataaaa gaacgtgcat tttcaagaag atttgaaatc acttgcccta aacggagttc atgtcccaat acaagatagg cttttccatg aggacgtggg acaatattaa ggcttatatc aatggtttgc gtattacgat acacttcctg aacagcatga acaagacttt ccaaaagcgg tgtcatatca acaatctctg cggtttctcg tgcaagctct gcatcgagcc gcgatgcatc agaaatatct gtaatcaacc gatcaagacg gcgcacatca tgttgaataa tttcaagcaa tttctcttgt gcttcttcat ttttagccag agggagcgtt tcaacagcac tgcgtagaga ggtgagagga ttttttaatt cgtggcttac atcagcagca aagcgttcaa tggtttcaat acgcatataa agggcattag tcatatcacg aatagaggtt gaaaggtgac caatttcatc ttcacgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg aagatgaaat tggtcacctt tcaacctcta ttcgtgatat gactaatgcc ctttatatgc gtattgaaac cattgaacgc tttgctgctg atgtaagcca cgaattaaaa aatcctctca cctctctacg cagtgctgtt gaaacgctcc ctctggctaa aaatgaagaa gcacaagaga aattgcttga aattattcaa catgatgtgc gccgtcttga tcggttgatt acagatattt ctgatgcatc gcggctcgat gcagagcttg cacgagaaac cgcagagatt gttgatatga caccgctttt ggaaagtctt gttcatgctg ttcaggaagt gtatcgtaat acgcaaacca ttgatataag ccttaatatt gtcccacgtc ctcatggaaa agcctatctt gtattgggac atgaactccg tttagggcaa gtgatttcaa atcttcttga aaatgcacgt tcttttattc ctcgtaatcg tggcaaaatt tgcattacta tgaaaagtca tgcttcaacc cttattttaa ccattgaaga taatggccca ggtattcgtt cagaaaatat tgaacgcatt tttgagcgtt tttatacaga tcgaccaaac gaagatgctt ttggacaaaa ctctggactt ggactttcta ttagtcggca aatcatcgaa gctcataatg ggacaattac agccgaaaat attattgatt ctacacttgg gaagtgtaaa actggtgcac gttttattat tatgcttccc ctcgctaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BagrA.01664.a.B3.GE36582

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	84
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRDGLIEAK IESLRTQGKI IAGAIAASAT VDTNSILIDP QKLLELQAGE SVTPTPQSTD TWDFPINPEQ IAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGIVSS YDLPAIETKQ SPWKRLYSWF SHALYGGGLA LDREQAKNNG DIYPEVYQAL NGSSATAQRR NKHGQLIVSV AVPVQRYRAV VGALLLSTLS SDIDNIVKGE R

Validated NT Sequence

attttntnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgaac caatttcgcg atggtttgat tgaagcaaaa attgaaagtt tgcgcaccca aggaaaaatc attgctgggg ctattgctgc ttctgcaaca gtagatacta attctatttt aattgatccc caaaaacttt tggaattaca agctggagaa agtgttacac caacacccca atcaaccgac acttgggatt ttcctattaa tccggagcaa atagctccac ttttacgccg ccttatcaca ccgaaaacaa caagagcacg tatttatgat cgagatgcca ctttgcttct tgattcacgt gttctttatt ccagtggaat agtttctagc tatgatcttc ccgctattga aacaaaacaa agtccctgga aacgcttata ttcatggttt tcacatgcgc tctatggtgg tggccttgct cttgatagag agcaagcaaa aaataatggt gatatctatc cagaagttta tcaagcatta aatggttctt ccgctactgc ccaacgacga aacaagcatg gacaactgat tgtttcggtt gctgttcctg tgcaacgcta tcgtgcagta gttggtgctc ttcttctttc gaccctcagc tcagacattg ataacattgt aaaaggtgaa agataagtga gtaagatagg atccggnnnn nnnnnnnccc cnnnnnnnnn nnngnnnnnn aaanaannnn nnnnnnnnnn gnnnngnnnn cnnnnnnnnn nnnnnncgcc nnnnnnnnnn nncnnnnnnn nnncatntnt ccnnnatnnn nnnncnannn nnnntngtnn nnntattcta gnncggnncn nnnnannnnc tnannnnntn naannnnnnn nnnancnncn ntnnnnannn ncagnnnnnn na

Expected Protein Sequence

MAHHHHHHMN QFRDGLIEAK IKSLCTQGKI IAGAIAASAT VDTNSILIDP QKLLELQTGE SVTPAPQSTD SWDFPINPEQ VAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGEVFS YDLPPLKTEQ SMWDRLTSWY SSTFYDKGIA LDREQTKTSG IAYPEVYRAL NGSLATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTTG SDIDNIVKGE R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc aatttcgcga tggtttaatt gaagcaaaaa ttaaaagttt atgtacccaa ggaaaaatta ttgctggggc tattgctgct tccgcaacag tagatacaaa ttctatcctc attgatcccc aaaaactctt agagttacag actggtgaaa gcgttacacc cgcccctcaa tcaaccgatt cttgggattt ccctattaac cctgagcaag ttgcccctct tttacggcgt ctcatcacac ctaaaacgac aagagcacgt atctatgatc gtgatgctac tctccttctt gattcgcgcg ttctttattc tagtggagaa gtttttagtt atgatttacc cccacttaaa acagaacaaa gcatgtggga tcgccttact tcatggtatt caagcacatt ttatgacaaa ggtattgctc ttgacagaga acaaacaaaa accagtggta ttgcttatcc agaagtgtac cgcgcattaa acggatctct tgctacggct aaaagacgca acagacaagg tcaattaatt gtttctgttg ccgttcctgt gcaacgctat cgcgcagtgg ttggagctct tcttctttca accactggtt ctgatattga taacattgta aaaggtgaaa gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat