SSGCID: Target Page

BagrA.01256.a

TrwD protein (Trw type IV secretion machinery)

CENTER ID:	BagrA.01256.a
ORGANISM:	Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BagrA.01256.a.B1.GE35686	full length		1	353

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|634504.3.peg.2114
RefSeq:	YP_002972719.1
UniProt:	C6AAT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVLSHVNP IQKDQAVFQL LQPLDCFLED PSITELSICR PCEVWTKSFK GWQVHDVPEL TTTFLQTLIT AIIVYNGMAP KSINYVLLPG GQRGTIIQTP AVVDGALSFV IRKHSLIVKT LEELKEEGAF DDFSDVSFNK PSQKEANNLL SKQDFTRLEP FEVQLLQHKR DGKILEFLQE CVFHKRNIII AGKTGSGKTT FARSLIEKVP VEERIITIED VHELFLPNHP NHVHMIYGNN VGRVSAEECL DACMRQSPDR IFLAELRGNE AWEYLNSLNT GHPGSITTTH ANSALQTFER CATLIKRSEV GRQLELEMIK IVLYTTVDVV LFFRDRKLSE VFYDPIFAKS KIA

NT Sequence

atgtctacag tcttgtcaca cgttaacccg atacagaaag atcaagcagt ttttcagctt ctacaaccgc ttgattgttt cttagaagat ccctcgatca ctgaattatc gatttgccgc ccctgtgaag tatggacgaa aagctttaag ggttggcaag tacatgacgt accagagtta acaaccactt ttttgcaaac gctcattaca gctattatcg tttataacgg tatggctccc aaaagtatta attatgtgct attgcctggt ggacaacgtg gtacaattat acaaacgcca gctgtcgttg atggtgctct ttcttttgta attcgtaaac attctcttat agttaagacg ttagaagaac tcaaagaaga aggtgcattt gatgatttct ccgacgtaag ttttaacaaa ccttcgcaaa aagaagcaaa taacttgtta tcgaagcagg acttcacacg gttggagcca tttgaagttc aacttttgca acataaacgt gatgggaaaa ttcttgaatt tttacaagaa tgtgtctttc acaaacgcaa cattattatt gcaggcaaga caggatctgg taaaacaaca tttgctcgct ctttaattga aaaggtccct gtagaggagc gtatcatcac aattgaagat gttcatgagc tttttctacc caatcatccc aatcatgtac atatgattta tggtaacaat gtaggccgtg tttcagctga agaatgccta gatgcgtgta tgcgtcagtc gcccgaccga atttttctcg ctgaattgcg aggtaatgaa gcatgggaat atctcaattc actcaacact ggtcatcccg gatcgattac aacaacgcat gccaacagcg ctttgcagac atttgaacga tgtgccacct tgattaaaag atcagaagtt ggccggcaac tcgagttaga aatgataaaa atcgttctct atacaacagt tgacgtagtg ttgttcttta gagatagaaa actctctgaa gtcttttacg acccgatttt tgctaaatca aaaattgctt aa

Details for BagrA.01256.a.B1.GE35686

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

aaaatcgggt cgtaaaagac ttcagagagt tttctatctc taaagaacaa cactacgtca actgttgtat agagaacgat ttttatcatt tctaactcga gttgccggcc aacttctgat cttttaatca aggtggcaca tcgttcaaat gtctgcaaag cgctgttggc atgcgttgtt gtaatcgatc cgggatgacc agtgttgagt gaattgagat attcccatgc ttcattacct cgcaattcag cgagaaaaat tcggtcgggc gactgacgca tacacgcatc taggcattct tcagctgaaa cacggcctac attgttacca taaatcatat gtacatgatt gggatgattg ggtagaaaaa gctcatgaac atcttcaatt gtgatgatac gctcctctac agggaccttt tcaattaaag agcgagcaaa tgttgtttta ccagatcctg tcttgcctgc aataataatg ttgcgtttgt gaaagacaca ttcttgtaaa aattcaagaa ttttcccatc acgtttatgt tgcaaaagtt gaacttcaaa tggctccaac cgtgtgaagt cctgcttcga taacaagtta tttgcttctt tttgcgaagg tttgttaaaa cttacgtcgg agaaatcatc aaatgcacct tcttctttga gttcttctaa cgtcttaact ataagagaat gtttacgaat tacaaaagaa agagcaccat caacgacagc tggcgtttgt ataattgtac cacgttgtcc accaggcaat agcacataat taatactttt gggagccata ccgttataaa cgataatagc tgtaatgagc gtttgcaaaa aagtggttgt taactctggt acgtcatgta cttgccaacc cttaaagctt ttcgtccata cttcacaggg gcggcaaatc gataattcag tgatcgaggg atcttctaag aaacaatcaa gcggttgtag aagctgaaaa actgcttgat ctttctgtat cgggttaacg tgtgacaaga ctgtagacat a

Expected Protein Sequence

MAHHHHHHMS TVLSHVNPIQ KDQAVFQLLQ PLDCFLEDPS ITELSICRPC EVWTKSFKGW QVHDVPELTT TFLQTLITAI IVYNGMAPKS INYVLLPGGQ RGTIIQTPAV VDGALSFVIR KHSLIVKTLE ELKEEGAFDD FSDVSFNKPS QKEANNLLSK QDFTRLEPFE VQLLQHKRDG KILEFLQECV FHKRNIIIAG KTGSGKTTFA RSLIEKVPVE ERIITIEDVH ELFLPNHPNH VHMIYGNNVG RVSAEECLDA CMRQSPDRIF LAELRGNEAW EYLNSLNTGH PGSITTTHAN SALQTFERCA TLIKRSEVGR QLELEMIKIV LYTTVDVVLF FRDRKLSEVF YDPIFAKSKI A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta cagtcttgtc acacgttaac ccgatacaga aagatcaagc agtttttcag cttctacaac cgcttgattg tttcttagaa gatccctcga tcactgaatt atcgatttgc cgcccctgtg aagtatggac gaaaagcttt aagggttggc aagtacatga cgtaccagag ttaacaacca cttttttgca aacgctcatt acagctatta tcgtttataa cggtatggct cccaaaagta ttaattatgt gctattgcct ggtggacaac gtggtacaat tatacaaacg ccagctgtcg ttgatggtgc tctttctttt gtaattcgta aacattctct tatagttaag acgttagaag aactcaaaga agaaggtgca tttgatgatt tctccgacgt aagttttaac aaaccttcgc aaaaagaagc aaataacttg ttatcgaagc aggacttcac acggttggag ccatttgaag ttcaactttt gcaacataaa cgtgatggga aaattcttga atttttacaa gaatgtgtct ttcacaaacg caacattatt attgcaggca agacaggatc tggtaaaaca acatttgctc gctctttaat tgaaaaggtc cctgtagagg agcgtatcat cacaattgaa gatgttcatg agctttttct acccaatcat cccaatcatg tacatatgat ttatggtaac aatgtaggcc gtgtttcagc tgaagaatgc ctagatgcgt gtatgcgtca gtcgcccgac cgaatttttc tcgctgaatt gcgaggtaat gaagcatggg aatatctcaa ttcactcaac actggtcatc ccggatcgat tacaacaacg catgccaaca gcgctttgca gacatttgaa cgatgtgcca ccttgattaa aagatcagaa gttggccggc aactcgagtt agaaatgata aaaatcgttc tctatacaac agttgacgta gtgttgttct ttagagatag aaaactctct gaagtctttt acgacccgat ttttgctaaa tcaaaaattg cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat