BagrA.00720.a

VirB11 protein (VirB type IV secretion machinery)

CENTER ID: BagrA.00720.a
ORGANISM: Bartonella grahamii as4aup
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BagrA.00720.a.B1.GE35684 full length 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_130713
PATRIC ID: fig|634504.3.peg.1550
RefSeq: YP_002972366.1
UniProt: C6AES2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSNLHTMSD ETIAIVLTKL EPIRAFLKDE SLFEIVINRP YQVMTEGIDG WKTIEAPALS FNELMGIAKV VASYSKQNIS DKNPILSATL PGNERIQVVI PPAVEKNTIS MTIRKPSSQS FSLEELANKG LFSLCEQISF TPLNDYLSRF NELKIIEHNL AHAYCNKDFV SFLNQAVKCQ KNILIAGKTG SGKTTLSKAL IAKIPQEERI ITIEDTQELV IPHPNHVSMF YSKDGQGLAS VGPKELLESS LRMRPDRILL QELRDGTAFY YIRNVNSGHP GSITTVHAST ALAAFEQMTL LVKESDGGGD LERDDIRGLL ISMIDIIIQC KRVEGKFKVT EIYYDPFKQR NIFGGD
NT Sequence
atgaactcaa acttacatac catgagcgat gaaaccatcg cgattgttct cacaaaactt gaacccatcc gtgctttttt gaaagatgaa agtctttttg aaattgttat caatcgtccc taccaagtaa tgacggaagg tattgatgga tggaaaacta tagaagcacc ggctctctct tttaatgagc ttatggggat tgcaaaagtt gtcgcgtcct attccaagca aaacatatca gacaagaatc caatattatc ggctactctg ccaggtaatg agcgcattca agttgttatt ccgccggctg tcgaaaaaaa cacgattagt atgacaattc gcaaaccatc atcgcaaagt ttttctctcg aagagctcgc gaataaaggt ctgttttcgc tctgtgaaca gatatctttc acgccattaa atgattatct ttctcgtttt aacgaactca aaatcatcga gcataactta gctcatgcct actgcaataa agactttgtc tcctttttaa atcaagctgt aaaatgccag aaaaatattt taattgcagg aaaaactggt tcgggtaaaa caacattatc aaaagcatta atcgccaaaa ttcctcaaga agagcgtatt attaccattg aagatacaca agaattggtg ataccgcacc ctaatcatgt ctcgatgttc tattcaaaag atggacaagg cttagcctct gttggaccca aagagttgct tgaatcatct ttacgaatgc gtcctgatcg tattcttttg caagaacttc gagatggtac agccttttat tatatccgca atgtcaattc aggtcatcca ggttccatta caacggttca tgcctcaaca gcccttgctg cctttgagca aatgaccctt ttggtcaaag aaagtgacgg aggaggtgat ttagagcgtg atgatattcg aggactgttg atttcaatga ttgatatcat catccaatgc aaacgggttg aaggaaagtt taaggtcaca gaaatttatt atgatccctt caaacaacga aacatctttg gaggagacta a
Details for BagrA.00720.a.B1.GE35684
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MSDETIAIVL TKLEPIRAFL KDESLFEIVI NRPYQVMTEG IDGWKTIEAP ALSFNELMGI AKVVASYSKQ NISDKNPILS ATLPGNERIQ VVIPPAVEKN TISMTIRKPS SQSFSLEELA NKGLFSLCEQ ISFTPLNDYL SRFNELKIIE HNLAHAYCNK DFVSFLNQAV KCQKNILIAG KTGSGKTTLS KALIAKIPQE ERIITIEDTQ ELVIPHPNHV SMFYSKDGQG LASVGPKELL ESSLRMRPDR ILLQELRDGT AFYYIRNVNS GHPGSITTVH ASTALAAFEQ MTLLVKESDG GGDLERDDIR GLLISMIDII IQCKRVEGKF KVTEIYYDPF
Validated NT Sequence
tgaagggatc ataataaatt tctgtgacct taaactttcc ttcaacccgt ttgcattgga tgatgatatc aatcattgaa atcaacagtc ctcgaatatc atcacgctct aaatcacctc ctccgtcact ttctttgacc aaaagggtca tttgctcaaa ggcagcaagg gctgttgagg catgaaccgt tgtaatggaa cctggatgac ctgaattgac attgcggata taataaaagg ctgtaccatc tcgaagttct tgcaaaagaa tacgatcagg acgcattcgt aaagatgatt caagcaactc tttgggtcca acagaggcta agccttgtcc atcttttgaa tagaacatcg agacatgatt agggtgcggt atcaccaatt cttgtgtatc ttcaatggta ataatacgct cttcttgagg aattttggcg attaatgctt ttgataatgt tgttttaccc gaaccagttt ttcctgcaat taaaatattt ttctggcatt ttacagcttg atttaaaaag gagacaaagt ctttattgca gtaggcatga gctaagttat gctcgatgat tttgagttcg ttaaaacgag aaagataatc atttaatggc gtgaaagata tctgttcaca gagcgaaaac agacctttat tcgcgagctc ttcgagagaa aaactttgcg atgatggttt gcgaattgtc atactaatcg tgtttttttc gacagccggc ggaataacaa cttgaatgcg ctcattacct ggcagagtag ccgataatat tggattcttg tctgatatgt tttgcttgga ataggacgcg acaacttttg caatccccat aagctcatta aaagagagag ccggtgcttc tatagttttc catccatcaa taccttccgt cattacttgg tagggacgat tgataacaat ttcaaaaaga ctttcatctt tcaaaaaagc acggatgggt tcaagttttg tgagaacaat cgcgatggtt tcatcgctca tggtatgta
Expected Protein Sequence
MAHHHHHHMN SNLHTMSDET IAIVLTKLEP IRAFLKDESL FEIVINRPYQ VMTEGIDGWK TIEAPALSFN ELMGIAKVVA SYSKQNISDK NPILSATLPG NERIQVVIPP AVEKNTISMT IRKPSSQSFS LEELANKGLF SLCEQISFTP LNDYLSRFNE LKIIEHNLAH AYCNKDFVSF LNQAVKCQKN ILIAGKTGSG KTTLSKALIA KIPQEERIIT IEDTQELVIP HPNHVSMFYS KDGQGLASVG PKELLESSLR MRPDRILLQE LRDGTAFYYI RNVNSGHPGS ITTVHASTAL AAFEQMTLLV KESDGGGDLE RDDIRGLLIS MIDIIIQCKR VEGKFKVTEI YYDPFKQRNI FGGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact caaacttaca taccatgagc gatgaaacca tcgcgattgt tctcacaaaa cttgaaccca tccgtgcttt tttgaaagat gaaagtcttt ttgaaattgt tatcaatcgt ccctaccaag taatgacgga aggtattgat ggatggaaaa ctatagaagc accggctctc tcttttaatg agcttatggg gattgcaaaa gttgtcgcgt cctattccaa gcaaaacata tcagacaaga atccaatatt atcggctact ctgccaggta atgagcgcat tcaagttgtt attccgccgg ctgtcgaaaa aaacacgatt agtatgacaa ttcgcaaacc atcatcgcaa agtttttctc tcgaagagct cgcgaataaa ggtctgtttt cgctctgtga acagatatct ttcacgccat taaatgatta tctttctcgt tttaacgaac tcaaaatcat cgagcataac ttagctcatg cctactgcaa taaagacttt gtctcctttt taaatcaagc tgtaaaatgc cagaaaaata ttttaattgc aggaaaaact ggttcgggta aaacaacatt atcaaaagca ttaatcgcca aaattcctca agaagagcgt attattacca ttgaagatac acaagaattg gtgataccgc accctaatca tgtctcgatg ttctattcaa aagatggaca aggcttagcc tctgttggac ccaaagagtt gcttgaatca tctttacgaa tgcgtcctga tcgtattctt ttgcaagaac ttcgagatgg tacagccttt tattatatcc gcaatgtcaa ttcaggtcat ccaggttcca ttacaacggt tcatgcctca acagcccttg ctgcctttga gcaaatgacc cttttggtca aagaaagtga cggaggaggt gatttagagc gtgatgatat tcgaggactg ttgatttcaa tgattgatat catcatccaa tgcaaacggg ttgaaggaaa gtttaaggtc acagaaattt attatgatcc cttcaaacaa cgaaacatct ttggaggaga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat