SSGCID: Target Page

BaduA.20981.a

bifunctional 3'5'-cyclic nucleotide phosphodiesterase/3'5'-cyclic nucleotide phosphodiesterase (BdWA1_002875)

CENTER ID:	BaduA.20981.a
ORGANISM:	Babesia duncani
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaduA.20981.a.UX2.GE45894	soluble domain		214	666
BaduA.20981.a.A3.GE45944	AF3 structured domain (PDEase)		335	621

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BdWA1_002875
UniProt:	A0AAD9PHZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHAKDPQCES FTESIFASKF LLFKCKALEN LYGLNVSEWV AQRSFLLTAC TMVLVLVSWV LGTISIQMGA FKAHQRPIHC VFHPLMLITL ILFMAPIISS RYKRHYAELV LLTTLGLVMA VWMGIATWLF IHLESPTSSL HAPVIVGGLL LIINVLVPTR FRYVWMLHLE FVLLSACAIV AKCTEIYTLW AGVTWHIIAH TATALLLLLG IYYNELHTRQ EFARWFYALN SRSCQNLVTR GSPVHQVGIL NEQQSSQMQT NPVCYKTSMQ HKGSLESMPT INVIPCKDLG ARGPLADNQL HATTTLESTL QSTARLNGNL YDWNFDILTT FKNRSNPLVA VGLELLKDFR SRYKIPQPKL AAALGALERL YNPNVPYHNA MHGAMVAQKV ACLSRLVGLH EYQNVLERAT IIIAALGHDA GHPGRTNAHL KTMRHPLAAI FGESSILEKF HASCTLSILR MEQYDILRYI PQHLVQGVRR DIVEYILATD MADHEPFLEH FQPIWTCKKF DMQANVGLVT KMVIKAADIS AATVNWEQSQ EWTWRLLAEF YTQGNVERNA GLPIIPLYDS NMQEDIAKLQ AQFFKGIVLP LYTLAFQKSA PTICMQQLQD NIKRWEKLQD SKAKLEPKQF NMQESCTIQL EWILPQLVGE LQSDHSHDAT IRDPRL

NT Sequence

atgcatgcaa aggatcccca gtgtgaatca ttcaccgagt ccatctttgc atcaaagttc cttttattca aatgtaaagc tctagagaat ttatatgggc tcaacgtatc ggaatgggta gcacaaaggt catttctatt gactgcatgc acaatggttt tggttttggt ttcttgggtc ttgggtacga tttcaataca aatgggagct ttcaaggcgc atcaacgccc aatccactgt gtattccatc cgcttatgct aattacatta atcctattta tggctccaat aatatcaagt cggtacaaac gccactatgc ggaactcgta ctacttacaa ctcttggatt ggtcatggcg gtttggatgg gcattgcgac gtggctcttt attcacttgg agtcacctac ttcaagtttg catgcacccg ttattgtggg ggggttactc ttgattataa atgtacttgt accaacaagg tttcgctatg tttggatgct acatttggaa tttgtattgc tatcggcttg tgctattgta gcaaaatgta cagaaattta cacgttatgg gcaggtgtaa catggcatat tatcgcccac acggccactg ctctcttgtt gctcttggga atctattaca atgagctcca tactcgccaa gagtttgctc gatggttcta cgctctcaat agtcgctctt gtcaaaatct cgtcacccgt ggatcacctg tacaccaagt tggaatatta aacgagcaac aatcgtccca aatgcaaacc aacccagtat gctacaaaac tagcatgcag cataaaggat ccctcgagtc catgccaacc atcaacgtca tcccttgcaa ggatttgggt gctcgcggac cactagcgga taatcaattg catgcaacca ccacactgga atcaacgctc cagtcgacgg cacgattaaa tggcaacttg tacgactgga attttgacat tttaaccaca tttaaaaatc gcagcaaccc cctcgttgca gttggtttgg aattgctcaa ggactttagg tcaaggtaca agataccgca accaaagctt gccgccgcgc ttggggctct agagcgactg tacaatccaa acgtgcccta tcacaatgca atgcatggcg ccatggtagc ccaaaaggta gcctgccttt ctagattggt tggtttgcat gagtaccaaa atgtcctgga acgagctaca attataatag cagcactagg acatgatgct ggacatcctg gacgtacaaa tgcacattta aagacaatgc ggcatccgct tgcagctata tttggagaat ctagtatatt ggaaaagttc catgcatcat gcaccctatc gatattaaga atggagcaat atgacatttt acgatacatc ccccagcatc tggtacaggg cgttcgcagg gatattgtag agtatattct agccactgat atggcggatc atgagccatt tctagaacat tttcaaccaa tatggacttg caaaaaattc gacatgcaag caaatgttgg gcttgtaacc aaaatggtaa ttaaagcagc ggatatatca gcagcaactg tcaattggga gcaaagtcaa gaatggacat ggcgtttatt ggctgaattt tatacccagg gaaatgtgga gcgcaatgca ggattgccaa tcattccatt atacgattca aacatgcaag aggatattgc aaaattacaa gcccaatttt tcaagggtat agtattgccg ctatatactc tagcgttcca aaaatcagcc ccaacaatat gcatgcaaca gttgcaagat aatattaaaa gatgggaaaa attacaagat tcaaaagcaa agctagaacc aaaacaattt aatatgcaag aatcgtgtac tatacagctc gagtggatac tccctcaact agtaggtgaa ttgcaaagtg atcactcaca tgatgcaaca atacgcgacc caaggctata g

Details for BaduA.20981.a.UX2.GE45894

HARVESTED ON:	4/24/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SNELHTRQEF ARWFYALNSR SCQNLVTRGS PVHQVGILNE QQSSQMQTNP VCYKTSMQHK GSLESMPTIN VIPCKDLGAR GPLADNQLHA TTTLESTLQS TARLNGNLYD WNFDILTTFK NRSNPLVAVG LELLKDFRSR YKIPQPKLAA ALGALERLYN PNVPYHNAMH GAMVAQKVAC LSRLVGLHEY QNVLERATII IAALGHDAGH PGRTNAHLKT MRHPLAAIFG ESSILEKFHA SCTLSILRME QYDILRYIPQ HLVQGVRRDI VEYILATDMA DHEPFLEHFQ PIWTCKKFDM QANVGLVTKM VIKAADISAA TVNWEQSQEW TWRLLAEFYT QGNVERNAGL PIIPLYDSNM QEDIAKLQAQ FFKGIVLPLY TLAFQKSAPT ICMQQLQDNI KRWEKLQDSK AKLEPKQFNM QESCTIQLEW ILPQLVGELQ SDHSHDATIR DPRL

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga acgaactgca cacacgtcag gaattcgccc gctggtttta tgccttaaac tcgcgttcat gccagaacct ggtaacgcgc ggctctccag tgcatcaggt cggtattctg aatgaacagc agtcatcaca gatgcagaca aatcccgttt gttataagac gtcaatgcaa cacaagggct ctcttgaaag catgcctaca ataaatgtaa ttccgtgtaa agacctggga gcacggggtc ctctggccga caaccagctg cacgccacta caaccttaga gtccactctg caaagcactg cccgcctgaa cgggaatctg tatgattgga acttcgatat ccttactact ttcaagaaca gatctaatcc attggtcgcg gtcggcctcg agcttctgaa agatttccgg agccgttata aaatccctca gcccaaatta gcagcggcac tcggagcgct ggaacgttta tataacccca atgtcccgta ccataacgcc atgcacgggg cgatggtggc tcagaaagtt gcttgtttaa gtcgtttagt cgggctgcac gaatatcaga acgtgttgga gcgcgccacc ataatcattg ccgcgttagg tcacgacgcc ggtcacccag gtcggaccaa cgcccacctt aaaaccatgc gccacccctt agcggcgatt ttcggtgaga gctcaatcct ggagaaattt cacgctagtt gtacactgtc tattcttcgc atggaacagt acgatatcct gcgttatatt cctcaacact tggttcaagg ggtccgtcgc gacatagtgg aatacatcct ggcaaccgac atggcagacc acgaaccttt ccttgagcac ttccagccga tttggacctg taagaagttt gatatgcagg ctaacgtagg tctggtgaca aagatggtta taaaggctgc agacattagc gcggcgacgg taaactggga acagtcgcag gagtggactt ggcgcctgct ggcggagttc tacactcaag gtaacgtaga acgtaacgcc gggttaccga taatcccgct gtatgactct aatatgcagg aagacatagc gaagctgcag gcacagttct ttaaagggat tgttcttcct ctgtacaccc tggcatttca gaagagcgca cctacgattt gtatgcagca actgcaggac aacataaagc gttgggagaa gctgcaggac agcaaggcta aactcgagcc gaagcagttc aacatgcagg agagctgcac catccaactg gaatggattc ttccgcagct ggttggagag ctgcagtccg accattcgca cgacgctacg attagagatc cgcgcctctg actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat

Details for BaduA.20981.a.A3.GE45944

HARVESTED ON:	6/18/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SSNPLVAVGL ELLKDFRSRY KIPQPKLAAA LGALERLYNP NVPYHNAMHG AMVAQKVACL SRLVGLHEYQ NVLERATIII AALGHDAGHP GRTNAHLKTM RHPLAAIFGE SSILEKFHAS CTLSILRMEQ YDILRYIPQH LVQGVRRDIV EYILATDMAD HEPFLEHFQP IWTCKKFDMQ ANVGLVTKMV IKAADISAAT VNWEQSQEWT WRLLAEFYTQ GNVERNAGLP IIPLYDSNMQ EDIAKLQAQF FKGIVLPLYT LAFQKSAPTI CMQQLQDNIK RWEKLQDS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgag caaccccctc gttgcagttg gtttggaatt gctcaaggac tttaggtcaa ggtacaagat accgcaacca aagcttgccg ccgcgcttgg ggctctagag cgactgtaca atccaaacgt gccctatcac aatgcaatgc atggcgccat ggtagcccaa aaggtagcct gcctttctag attggttggt ttgcatgagt accaaaatgt cctggaacga gctacaatta taatagcagc actaggacat gatgctggac atcctggacg tacaaatgca catttaaaga caatgcggca tccgcttgca gctatatttg gagaatctag tatattggaa aagttccatg catcatgcac cctatcgata ttaagaatgg agcaatatga cattttacga tacatccccc agcatctggt acagggcgtt cgcagggata ttgtagagta tattctagcc actgatatgg cggatcatga gccatttcta gaacattttc aaccaatatg gacttgcaaa aaattcgaca tgcaagcaaa tgttgggctt gtaaccaaaa tggtaattaa agcagcggat atatcagcag caactgtcaa ttgggagcaa agtcaagaat ggacatggcg tttattggct gaattttata cccagggaaa tgtggagcgc aatgcaggat tgccaatcat tccattatac gattcaaaca tgcaagagga tattgcaaaa ttacaagccc aatttttcaa gggtatagta ttgccgctat atactctagc gttccaaaaa tcagccccaa caatatgcat gcaacagttg caagataata ttaaaagatg ggaaaaatta caagattcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa