BaduA.20782.a

BdWA1_000266/7; hypothetical protein; putative type Ia membrane protein

CENTER ID: BaduA.20782.a
ORGANISM: Babesia duncani
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaduA.20782.a.MB21.PW39369 Ben Mamoun construct 20 483

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroPlasmaDB:BdWA1_000266

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFLWLLGFA IIYRKFVVGV GPDEDSDYPE VDVKSSKVNI GISATVDKFF DDMKLMEEDY KAYQDKILGA LNTVRRRLEK AKHLEATDLS DLANIMLDVN KELTKMNSCV SRLAALRPYV EKSINLLHED DKETAKKRLL NFMNPDEMVN VLHMLFSEYK ELNVELVHVK KRGTAPSQSE SLSASQSESL SKSKIESLSK SLSALNQEPG RILEPALAIY DKIMNGGTEI LEEMNKLNLK IQGKQTMSSM EYLYIVQNML HAKEYVMESK QPLTSLGYLG TALDQMQFTY SPTEKSHVEN IKKEVGEILN GIKDFQNKVK NSIDTVEKRV TTISVTDALP KERALEIISA VPAYVQIFKK QMDELKGAVL NDINELDKQL DEKKRIPSKE HEQMEAKIPI LETQVQFFLE FIEAMKYFRV TCEMIPKMMS VDEKKQFRRE LISCDMALKN EKQEYDFMIE QFKKVKERIV QTRPRASRFK RKHSGFSTME PSSLLLVLPV IVSLFY
NT Sequence
atggattttt tgtggttact tggattcgca attatttaca ggaaatttgt cgtgggcgtt ggccctgatg aggactctga ttatcctgaa gtggatgtaa aatcgtcgaa agtaaatatc ggaatatctg caactgttga taaatttttt gatgatatga aattaatgga ggaggattac aaggcatatc aggataagat attaggtgct ttaaatactg taagaaggag acttgaaaaa gcaaaacacc tagaagctac tgatctctct gatttagcca atataatgct tgacgtaaat aaagaattaa cgaaaatgaa ttcatgtgtt tccaggttgg cagcattgcg cccatatgta gagaaaagca taaatctttt gcatgaagat gacaaggaaa cggcaaaaaa aaggttatta aactttatga atccggatga aatggttaat gttttgcaca tgttattttc agaatataag gaattaaatg tagagttagt tcatgttaag aaacgtggta ccgcaccaag ccaatcagaa agtctttcag caagccaatc agaaagtctt tcaaaaagta aaattgaaag cctttcaaaa agtctttcag ccttgaacca agaacctggt agaattttag aaccagcctt ggctatctac gataaaatta tgaatggagg aacggagata cttgaggaaa tgaataaatt aaatctaaaa atccaaggga aacagacaat gtctagtatg gaatatttat atatcgtaca gaatatgtta catgcaaaag aatatgttat ggaatcaaaa cagcctctta cttccttagg gtatttggga actgcactag accaaatgca gtttacttat tcacccactg aaaaatcaca tgtagagaac ataaaaaaag aagttggtga aattttaaat ggtattaaag actttcaaaa taaggtaaaa aacagcattg ataccgtaga aaaacgcgtt acaacaatat cagtgaccga cgcattaccg aaggaacgtg cattggaaat aatatctgcg gtaccggcct atgtgcaaat ttttaaaaaa caaatggatg agctaaaagg tgctgtgtta aacgatataa atgaactaga taaacaatta gatgaaaaga aaagaattcc ctcaaaagag catgagcaaa tggaagcaaa aatacccatt ttagaaaccc aagtacaatt ttttttggaa ttcatagaag caatgaagta tttcagagta acatgtgaaa tgattccaaa aatgatgagt gttgatgaaa agaaacaatt tagacgagag ctgatttcat gtgatatggc tttaaaaaat gaaaaacagg aatacgactt catgatagaa caatttaaaa aagtcaaaga acgcatagtc caaacaagac caagagcttc acgattcaaa aggaaacata gtggtttttc aacaatggaa ccatccagtt tgttattggt gcttcccgtt attgtctctt tgttctatta a
Details for BaduA.20782.a.MB21.PW39369
PURIFICATION DATe: 1/10/2025
CONCENTRATION: 15.1mg/ml
OBSERVED MW: 99kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS VGPDEDSDYP EVDVKSSKVN IGISATVDKF FDDMKLMEED YKAYQDKILG ALNTVRRRLE KAKHLEATDL SDLANIMLDV NKELTKMNSC VSRLAALRPY VEKSINLLHE DDKETAKKRL LNFMNPDEMV NVLHMLFSEY KELNVELVHV KKRGTAPSQS ESLSASQSES LSISQSESLS KSKIESLSKS LSALNQEPGR ILEPALAIYD KIMNGGTEIL EEMNKLNLKI QGKQTMSSME YLYIVQNMLH AKEYVMESKQ PLTSLGYLGT ALDQMQFTYS PTEKSHVENI KKEVGEILNG IKDFQNKVKN SIDTVEKRVT TISVTDALPK ERALEIISAV PAYVQIFKKQ MDELKGAVLN DINELDKQLD EKKRIPSKEH EQMEAKIPIL ETQVQFFLEF IEAMKYFRVT CEMIPKMMGV DEKKQFRREL ILCDMALKNE KQEYDFMIEQ FKKVKERIVQ TRPRASRFKR KH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAACAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTCGG TCCTGACGAG GACAGCGACT ACCCCGAGGT GGACGTGAAG AGCAGCAAGG TGAACATCGG CATCAGCGCC ACCGTGGACA AATTCTTCGA CGACATGAAA CTGATGGAAG AGGACTACAA GGCCTACCAG GACAAAATCC TGGGAGCCCT GAACACAGTG CGGCGGAGAC TCGAGAAGGC CAAGCACCTG GAAGCCACAG ACCTGTCTGA TCTGGCTAAT ATCATGCTGG ATGTGAACAA GGAACTGACA AAGATGAATA GCTGCGTGTC CAGACTGGCC GCCCTGAGAC CCTACGTGGA AAAATCCATC AACCTGCTGC ATGAGGATGA TAAGGAAACC GCCAAAAAGA GACTGCTGAA TTTCATGAAC CCCGATGAGA TGGTGAACGT GCTGCACATG CTGTTCAGCG AGTACAAGGA ACTGAACGTG GAACTCGTGC ACGTGAAGAA GCGGGGCACA GCCCCTTCTC AGAGCGAGAG CCTGAGCGCT AGCCAGTCCG AGTCACTGTC CATCTCTCAG AGCGAATCCC TGAGCAAATC TAAGATCGAG TCTCTGAGCA AGTCCCTGAG CGCCCTCAAC CAGGAGCCCG GCAGAATCCT GGAACCTGCT CTGGCCATCT ACGACAAGAT CATGAACGGA GGAACAGAGA TCCTGGAAGA GATGAACAAG CTGAACCTGA AGATCCAGGG CAAGCAGACC ATGAGCTCTA TGGAATACCT GTACATCGTG CAAAACATGC TGCACGCCAA GGAGTACGTG ATGGAATCTA AGCAACCTCT GACCAGCCTG GGCTACCTGG GCACCGCCCT GGATCAGATG CAGTTCACCT ACAGCCCCAC CGAGAAGTCC CACGTGGAAA ACATCAAGAA GGAAGTGGGC GAGATCCTGA ATGGCATCAA GGACTTCCAG AACAAGGTGA AGAACAGCAT CGACACAGTG GAGAAGCGGG TGACCACCAT CAGCGTGACC GACGCCCTCC CCAAGGAACG GGCCCTGGAA ATCATCAGCG CTGTGCCCGC CTATGTGCAG ATCTTCAAGA AACAGATGGA CGAGCTCAAG GGCGCCGTGC TGAACGACAT CAACGAGCTG GACAAGCAGC TGGACGAGAA AAAAAGAATC CCTAGCAAGG AGCACGAGCA GATGGAAGCC AAGATCCCCA TCCTGGAAAC CCAGGTGCAG TTTTTCCTGG AATTCATCGA AGCTATGAAG TACTTCAGAG TTACCTGCGA GATGATCCCT AAGATGATGG GCGTGGACGA GAAGAAGCAG TTTAGAAGGG AACTGATCCT TTGTGACATG GCCCTGAAGA ACGAGAAACA GGAGTATGAT TTCATGATCG AGCAGTTCAA GAAAGTGAAA GAGAGAATAG TTCAAACAAG ACCTAGAGCC AGCAGATTCA AGAGAAAGCA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA