SSGCID: Target Page

BaduA.19515.a

bifunctional HD-PDEase domain/3'5'-cyclic nucleotide phosphodiesterase/3'5'-cyclic nucleotide phosphodiesterase

CENTER ID:	BaduA.19515.a
ORGANISM:	Babesia duncani
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaduA.19515.a.UX1.GE45893	full length		1	498
BaduA.19515.a.A2.GE45943	AF3 structured domain (PDEase)		152	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BdWA1_002302
UniProt:	A0AAD9PJ85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHNTSGIDRV LIRSAVLSVD RYLKQFDDND DICESFEIIK KALVVFDHSR VSYSICGTAL SEFLTLESLS LNSEDISPVN QGNLASFVSR NACTSAATIC SGFDPLQSCK GSKVHRCDDD VDPLSRMNSA NVGPITPGWS IGKSRRTPTT LDTEQMYQKY IGQLGRNWNL DLLALSNLPQ VASAGPIYCM GAALLKDIGE RIHPEFNNLL PPVLKCLQDV YLPNPYHNAL HAATVGHMAK ILASCIGADQ RLEGYEQFAF IMAALSHDAG HPGRTNAFLS QTGNPLAIVY NDNSILENYH AFLAFNIMRS HADFYKIFTG EQWVQIRKRM IQLILATDMK LHFTHVNSVK ERRQSGLFSL DNKDDFWLVL VLCIKAGDVG HNFLPWVDHL VWTHVLFQEF YLQGDEEHLL SMPLLLFFDR TKAADIPQSQ LGFFQGFTKP MLEELIVYDT SPQGYISNVI LKNAMQNLDM WARHSNKTIP QILNEPHDDA QEPPEALF

NT Sequence

atgcataata catctggaat agatcgagtg ctaatcaggt cagctgttct atctgtagat cgctatttga aacaatttga cgacaatgat gatatttgcg agtcttttga gattataaaa aaggctcttg tagtctttga ccacagccga gtaagctata gcatttgtgg aaccgcattg tctgaattcc tgaccctcga atctttgtcc ttgaactctg aagatatatc accagttaac caaggaaact tggcatcctt tgtttctcgt aatgcatgca ctagtgcggc aaccatatgt agcggttttg atcccttgca aagctgtaaa ggctcaaaag tacatagatg cgatgatgat gttgatccac tctctagaat gaattctgca aacgtgggcc caattactcc tggatggtcc attggtaaaa gtcggagaac cccgactacc cttgatacgg aacaaatgta ccaaaaatac attggccagt tgggtcgaaa ttggaatttg gatttattag ccctttcaaa cctcccgcaa gttgcaagtg ctggaccaat ttattgtatg ggagccgcac tattaaaaga cattggagaa cgcattcacc cggaatttaa caatctatta ccccccgttt taaagtgcct acaggatgta tatttaccaa acccctatca caatgcgttg catgccgcaa ctgtgggaca tatggcgaaa attctagctt cgtgcattgg cgcggatcag aggttggagg gttatgaaca gtttgctttt attatggccg cgttaagtca tgatgcgggg catccgggcc gaacaaacgc ctttttatcc caaactggga atcctctggc aatcgtgtac aatgataata gcatactgga aaactatcac gcctttctcg cttttaatat tatgcgttca cacgctgatt tttacaagat attcactggg gaacaatggg tccaaattcg caagcgcatg attcagttga ttctagctac ggatatgaag cttcatttta cccatgtaaa ttccgtcaag gaacggcgtc agagtggact atttagtttg gataataagg acgacttttg gttagtactg gtcttgtgta ttaaagctgg cgatgttggc cataactttt tgccatgggt tgatcatttg gtttggaccc atgttctttt tcaagaattc tatctccagg gagatgagga acatttgtta tcaatgccct tgttgttgtt tttcgacagg acaaaagctg cggatattcc gcagtcacaa ttgggatttt ttcagggttt taccaagcca atgctagagg agcttattgt atatgatact agtccacaag gttatatttc aaatgttatt ttgaaaaatg ccatgcaaaa tttggatatg tgggctcgcc attcaaacaa gacgattcca caaattttaa atgaacccca tgatgatgct caagagccac cagaggctct tttttaa

Details for BaduA.19515.a.UX1.GE45893

HARVESTED ON:	4/24/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHNTSGIDR VLIRSAVLSV DRYLKQFDDN DDICESFEII KKALVVFDHS RVSYSICGTA LSEFLTLESL SLNSEDISPV NQGNLASFVS RNACTSAATI CSGFDPLQSC KGSKVHRCDD DVDPLSRMNS ANVGPITPGW SIGKSRRTPT TLDTEQMYQK YIGQLGRNWN LDLLALSNLP QVASAGPIYC MGAALLKDIG ERIHPEFNNL LPPVLKCLQD VYLPNPYHNA LHAATVGHMA KILASCIGAD QRLEGYEQFA FIMAALSHDA GHPGRTNAFL SQTGNPLAIV YNDNSILENY HAFLAFNIMR SHADFYKIFT GEQWVQIRKR MIQLILATDM KLHFTHVNSV KERRQSGLFS LDNKDDFWLV LVLCIKAGDV GHNFLPWVDH LVWTHVLFQE FYLQGDEEHL LSMPLLLFFD RTKAADIPQS QLGFFQGFTK PMLEELIVYD TSPQGYISNV ILKNAMQNLD MWARHSNKTI PQILNEPHDD AQEPPEALF

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgcacaacac gtccggtatt gaccgcgtct taattcgtag cgcagtgtta agcgtggacc gatacctcaa gcagttcgat gataacgacg acatctgtga aagcttcgaa atcatcaaga aagccttagt tgtgttcgat cattcgcgcg tgtcttactc catatgcggc actgcgctga gtgagtttct cacacttgag agtctgagcc ttaatagcga ggacatctcc ccggtgaatc agggcaatct cgcgagtttc gtaagcagaa acgcctgtac atccgcagct acaatttgct ccggattcga ccctctccag tcatgcaagg gttccaaggt ccaccgttgt gacgacgacg tcgaccccct ttcacggatg aactcggcta atgttggacc tataacgcca ggttggtcta taggaaagtc cagacgcacg ccaacgacgc tggacacaga gcagatgtat cagaagtata tcggtcaact gggaagaaac tggaacctgg acctgctggc tctcagcaat ttgccccagg tggccagcgc gggtccgatc tactgcatgg gagcggcttt acttaaggat atcggtgagc ggatccatcc agagttcaat aaccttctcc caccagtact gaaatgttta caagacgttt acttaccgaa tccgtaccat aacgccctgc acgctgctac cgtcgggcac atggcaaaga tcttggccag ctgtataggt gcagaccaac gcttagaagg ctacgagcaa ttcgcgttca taatggcggc tctgagccac gacgcaggtc accccgggcg gaccaatgca ttcctttcac agaccggaaa cccgctcgct attgtctata acgacaactc gatcttggag aattaccatg cattcctggc attcaacatc atgcggagtc atgccgactt ctataaaatt ttcaccggcg agcagtgggt acagatccgt aaacgaatga tacaactgat cctcgccaca gacatgaaac tgcacttcac gcacgtgaac tcagtgaaag agcgccgcca atctggtctg ttttccttag acaacaaaga tgatttctgg ctggtccttg tactgtgcat aaaggccggt gacgtaggac acaatttctt accgtgggtg gaccacctgg tatggactca cgtcttattc caggagtttt acctgcaagg tgacgaagag caccttttga gcatgccgct gcttttattc tttgatcgta cgaaggccgc tgacatccct caatctcagc tggggttctt ccaaggcttc acaaaaccca tgctggaaga actgatcgtc tacgacacca gcccgcaggg gtacatatct aacgtcatcc tgaagaacgc gatgcagaac ttagacatgt gggcccgaca cagtaataaa acaatcccgc agatacttaa cgagccgcac gacgacgccc aggaaccccc ggaagccctg ttctgactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat

Details for BaduA.19515.a.A2.GE45943

HARVESTED ON:	6/18/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SDTEQMYQKY IGQLGRNWNL DLLALSNLPQ VASAGPIYCM GAALLKDIGE RIHPEFNNLL PPVLKCLQDV YLPNPYHNAL HAATVGHMAK ILASCIGADQ RLEGYEQFAF IMAALSHDAG HPGRTNAFLS QTGNPLAIVY NDNSILENYH AFLAFNIMRS HADFYKIFTG EQWVQIRKRM IQLILATDMK LHFTHVNSVK ERRQSGLFSL DNKDDFWLVL VLCIKAGDVG HNFLPWVDHL VWTHVLFQEF YLQGDEEHLL SMPLLLFFDR TKAADIPQSQ LGFFQGFTKP MLEELIVYDT SPQGYISNVI LKNAMQNLDM WARHSNKTIP QILNE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgga tacggaacaa atgtaccaaa aatacattgg ccagttgggt cgaaattgga atttggattt attagccctt tcaaacctcc cgcaagttgc aagtgctgga ccaatttatt gtatgggagc cgcactatta aaagacattg gagaacgcat tcacccggaa tttaacaatc tattaccccc cgttttaaag tgcctacagg atgtatattt accaaacccc tatcacaatg cgttgcatgc cgcaactgtg ggacatatgg cgaaaattct agcttcgtgc attggcgcgg atcagaggtt ggagggttat gaacagtttg cttttattat ggccgcgtta agtcatgatg cggggcatcc gggccgaaca aacgcctttt tatcccaaac tgggaatcct ctggcaatcg tgtacaatga taatagcata ctggaaaact atcacgcctt tctcgctttt aatattatgc gttcacacgc tgatttttac aagatattca ctggggaaca atgggtccaa attcgcaagc gcatgattca gttgattcta gctacggata tgaagcttca ttttacccat gtaaattccg tcaaggaacg gcgtcagagt ggactattta gtttggataa taaggacgac ttttggttag tactggtctt gtgtattaaa gctggcgatg ttggccataa ctttttgcca tgggttgatc atttggtttg gacccatgtt ctttttcaag aattctatct ccagggagat gaggaacatt tgttatcaat gcccttgttg ttgtttttcg acaggacaaa agctgcggat attccgcagt cacaattggg attttttcag ggttttacca agccaatgct agaggagctt attgtatatg atactagtcc acaaggttat atttcaaatg ttattttgaa aaatgccatg caaaatttgg atatgtgggc tcgccattca aacaagacga ttccacaaat tttaaatgaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa