SSGCID: Target Page

BaduA.01191.a

BdWA1_001462-T1; Dihydrofolate reductase-thymidylate synthase (BdDHFR-TS)

CENTER ID:	BaduA.01191.a
ORGANISM:	Babesia duncani
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaduA.01191.a.MB11.GE45936	full length		1	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BdWA1_001462

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLSTKYEGF SPIVMFVATD VKGGIGFEGK IPWPHIPMDF SFFFRGTCYV EPEILNRYPE IQNVVIFGRK TYESIPANVF PLKKRHNVII SRTLTHVPGA SVFNNLDDAL RWVNESKRFH KTIIMGGVEI YKLALETGLV EKIYLTRIGN EFKADRFFPD ITKDFEIVGI SQTFGTDFTT FDFVIYEKKG LNQLDHVTKS FDEMLLTGKP LKTVAPLYKA CPNIRIRYHQ EFQYLDICAD VLSTGTLKEN RTGIDALSKF GYQMRFDLSQ SFPLLTTKKV FLRGITEELI WFINGSTNGN DLLKKNVRIW ELNGKRSFLD NLGFYNREEH DLGPVYGFQW RHFGATYTDM HADYKGQGID QLVNVINSIK NDPNSRRLIV CSWNVSDVPK MALPPCHLLF QFYVAQGKLS CMLHQRSCDL GLGVPFNIAS YSILTAMIAQ VCNLQLGEFV HNLADAHIYV NHIDALKEQL TRVPYPFPLL KLNKDITDIC DFKLEDVKVE GYSCHPTIKM EMAA

NT Sequence

atggacttaa gtaccaaata cgaggggttc agtcccattg ttatgttcgt tgccactgat gttaaaggag gaattggatt tgaaggtaaa attccatggc cacacatccc tatggatttc tcctttttct ttagaggaac ttgttacgtt gagcccgaaa tcctgaatcg gtacccagag atccagaatg tcgttatatt tgggcgtaag acatacgaga gcattcccgc taatgtattc cctctaaaga aacgtcacaa tgtcatcatt agcagaacat tgactcatgt gcccggcgct tctgtgttta ataatctaga cgatgcgctc cgatgggtaa acgaatcgaa aaggtttcac aaaacaatca tcatgggtgg agttgaaatc tacaagctgg cattggaaac tgggctcgtg gaaaagattt accttacccg aattggtaat gaatttaaag cagatcgatt ctttcccgat atcaccaaag attttgaaat tgtgggcatt agtcagactt ttggaaccga tttcacaacg tttgattttg taatttacga aaaaaagggt ttgaatcaat tggatcatgt taccaaatca tttgatgaaa tgttgctgac gggaaagcca ttaaaaaccg tggctccact atacaaggca tgcccaaaca ttagaattcg atatcaccaa gagtttcagt atcttgatat ctgtgcagat gtattaagta ctggtacatt aaaggaaaat cgaacaggta tagatgctct atctaaattt gggtatcaaa tgagatttga tttatctcaa tcatttccac tccttacaac taaaaaggta ttcttacgtg gaatcactga agaattgatt tggtttatta atggctctac caatggcaat gatttgttaa agaaaaatgt acgcatttgg gaactaaatg gtaaacgttc cttccttgat aatctcgggt tttacaatcg agaagaacac gatttgggtc ccgtttacgg ctttcaatgg cgccactttg gggcaaccta cacggatatg catgctgatt acaagggaca aggaatagat caactggtga atgtaattaa ttcgataaag aacgatccaa acagtcgccg tttgattgtt tgttcctgga atgtcagtga tgtaccaaaa atggcactac caccatgtca tttactattt caattttatg ttgcacaagg gaaactttca tgcatgttac atcaaagatc atgcgattta ggacttggag taccgtttaa tattgcttca tatagcatac ttacagcgat gattgcccag gtatgcaatt tacaacttgg ggaattcgtg cacaacttgg ctgatgcgca tatttacgtt aaccatatag acgctttaaa ggaacaattg acgcgcgttc cgtacccatt cccattgctc aaactcaaca aggacattac ggacatttgt gactttaagc tcgaggatgt caaggtcgag ggatacagct gccatcccac cattaagatg gaaatggccg cctaa

Details for BaduA.01191.a.MB11.GE45936

HARVESTED ON:	6/18/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	104kDa
OBSERVED MW:	104kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS DLSTKYEGFS PIVMFVATDV KGGIGFEGKI PWPHIPMDFS FFFRGTCYVE PEILNRYPEI QNVVIFGRKT YESIPANVFP LKKRHNVIIS RTLTHVPGAS VFNNLDDALR WVNESKRFHK TIIMGGVEIY KLALETGLVE KIYLTRIGNE FKADRFFPDI TKDFEIVGIS QTFGTDFTTF DFVIYEKKGL NQLDHVTKSF DEMLLTGKPL KTVAPLYKAC PNIRIRYHQE FQYLDICADV LSTGTLKENR TGIDALSKFG YQMRFDLSQS FPLLTTKKVF LRGITEELIW FINGSTNGND LLKKNVRIWE LNGKRSFLDN LGFYNREEHD LGPVYGFQWR HFGATYTDMH ADYKGQGIDQ LVNVINSIKN DPNSRRLIVC SWNVSDVPKM ALPPCHLLFQ FYVAQGKLSC MLHQRSCDLG LGVPFNIASY SILTAMIAQV CNLQLGEFVH NLADAHIYVN HIDALKEQLT RVPYPFPLLK LNKDITDICD FKLEDVKVEG YSCHPTIKME MAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggatct gagcaccaag tacgagggct ttagcccgat tgtgatgttc gttgcgaccg acgtgaaggg tggcatcggt ttcgaaggca aaatcccgtg gccgcacatt ccgatggatt ttagcttctt tttccgtggc acctgctacg ttgagccgga aattctgaac cgttatccgg agatccagaa cgtggttatt ttcggtcgta aaacctatga aagcatcccg gcgaacgtgt ttccgctgaa gaaacgtcac aacgttatca ttagccgtac cctgacccat gtgccgggtg cgagcgtttt caacaacctg gacgatgcgc tgcgttgggt gaacgagagc aagcgttttc acaaaaccat cattatgggt ggcgtggaaa tctacaagct ggcgctggag accggcctgg ttgaaaaaat ctatctgacc cgtattggta acgagttcaa ggcggaccgt ttcttcccgg atattaccaa agactttgaa atcgttggta ttagccagac ctttggcacc gatttcacca cctttgactt cgtgatctac gagaagaaag gcctgaacca actggatcac gttaccaaga gcttcgacga aatgctgctg accggcaagc cgctgaaaac cgtggcgccg ctgtataaag cgtgcccgaa catccgtatt cgttaccacc aggagttcca atatctggat atttgcgcgg acgttctgag caccggtacc ctgaaggaaa accgtaccgg catcgatgcg ctgagcaaat ttggttacca gatgcgtttc gacctgagcc aaagctttcc gctgctgacc accaagaaag tgttcctgcg tggcattacc gaggaactga tctggtttat taacggtagc accaacggca acgatctgct gaagaaaaac gttcgtatct gggagctgaa cggcaagcgt agcttcctgg ataacctggg tttttacaac cgtgaggaac acgacctggg tccggtgtat ggctttcagt ggcgtcactt cggtgcgacc tacaccgata tgcacgcgga ctataagggt cagggcattg atcaactggt gaacgttatc aacagcatta aaaacgaccc gaacagccgt cgtctgatcg tttgcagctg gaacgtgagc gatgttccga aaatggcgct gccgccgtgc cacctgctgt ttcagttcta cgtggcgcaa ggcaaactga gctgcatgct gcaccagcgt agctgcgacc tgggtctggg cgttccgttc aacatcgcga gctatagcat tctgaccgcg atgatcgcgc aggtgtgcaa cctgcaactg ggcgagtttg ttcacaacct ggcggatgcg cacatttacg tgaaccacat cgacgcgctg aaggaacaac tgacccgtgt tccgtatccg ttcccgctgc tgaagctgaa caaagacatc accgatattt gcgactttaa gctggaggac gtgaaagttg aaggttacag ctgccacccg accatcaaaa tggaaatggc ggcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa