SSGCID: Target Page

BaclA.18404.b

Effector protein yopJ (Virulence factor yopJ) (Yop-like family protein)

CENTER ID:	BaclA.18404.b
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.18404.b.B1.GE35682	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.916
RefSeq:	YP_004159216.1
UniProt:	E6YIF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRLFNSIDR ASDSFITEEN DIAREALEGL ITRLESDIID GSWMTKGYMD IDFKMMPALV KQANNKYPEM NLKLAMTFED LCVSVQKTID DGIQSSRFIT NILNRSMPHF VVIDLKTIDD KTSLVLFEAT KLDHKISKIL ISRMKRVIEA SQLPSCYYSI VEMDIQRSFS ECGIFSLALA KKLYHEAEKL ERIHKDNIDG VLCDCESYLP YDKLDTYLPV TFYKHAQGIS RINEYVKSNP EAANKIINKK NETIFERFDR NSIVVNKKNV SVSSHRKRIS EYRSLIR

NT Sequence

atgttgcggt tattcaattc aattgatcga gccagtgatt cattcataac tgaggaaaat gatatcgcaa gagaggcatt agaaggtctt attactcgtt tggagagtga tatcattgat ggtagttgga tgacaaaagg ttatatggat atagatttta aaatgatgcc tgctcttgta aagcaggcaa acaataaata tccagagatg aatcttaaac ttgctatgac atttgaagat ctttgcgtat cagtacaaaa aactatcgat gatgggattc aatcttcaag gtttataacg aatatactga ataggagtat gccccatttt gtggtaattg acttaaaaac catcgatgac aagacttcct tggtattatt tgaggctaca aaacttgatc ataagatttc aaagatactt atatcgagaa tgaaaagagt cattgaagct tcccaattgc cttcttgtta ctattccatt gtggaaatgg atattcagag aagtttctct gagtgtggaa tttttagttt ggctcttgct aaaaagctgt atcatgaggc agaaaagttg gaaagaatac acaaagataa tattgacggt gttttatgtg attgcgagtc atatttacct tatgataagt tggatacata tcttccagtt actttttaca aacatgcaca aggtatcagt cgtattaatg aatatgtgaa atcgaatcca gaggctgcaa ataaaattat taataaaaaa aatgaaacta tttttgaaag gtttgataga aattcaattg tggtcaataa gaaaaatgta tctgtttcat cacatagaaa aaggatttct gaatatagat cattaataag atga

Details for BaclA.18404.b.B1.GE35682

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML RLFNSIDRAS DSFITEENDI AREALEGLIT RLESDIIDGS WMTKGYMDID FKMMPALVKQ ANNKYPEMNL KLAMTFEDLC VSVQKTIDDG IQSSRFITNI LNRSMPHFVV IDLKTIDDKT SLVLFEATKL DHKISKILIS RMKRVIEASQ LPSCYYSIVE MDIQRSFSEC GIFSLALAKK LYHEAEKLER IHKDNIDGVL CDCESYLPYD KLDTYLPVTF YKHAQGISRI NEYVKSNPEA ANKIINKKNE TIFERXDRNS IVVNKKNVSV SSHRKXXSEY RSLIX

Validated NT Sequence

taactttaan aaggaganat accatggctc accaccacca ccaccatatg ttgcggttat tcaattcaat tgatcgagcc agtgattcat tcataactga ggaaaatgat atcgcaagag aggcattaga aggtcttatt actcgtttgg agagtgatat cattgatggt agttggatga caaaaggtta tatggatata gattttaaaa tgatgcctgc tcttgtaaag caggcaaaca ataaatatcc agagatgaat cttaaacttg ctatgacatt tgaagatctt tgcgtatcag tacaaaaaac tatcgatgat gggattcaat cttcaaggtt tataacgaat atactgaata ggagtatgcc ccattttgtg gtaattgact taaaaaccat cgatgacaag acttccttgg tattatttga ggctacaaaa cttgatcata agatttcaaa gatacttata tcgagaatga aaagagtcat tgaagcttcc caattgcctt cttgttacta ttccattgtg gaaatggata ttcagagaag tttctctgag tgtggaattt ttagtttggc tcttgctaaa aagctgtatc atgaggcaga aaagttggaa agaatacaca aagataatat tgacggtgtt ttatgtgatt gcgagtcata tttaccttat gataagttgg atacatatct tccagttact ttttacaaac atgcacaagg tatcagtcgt attaatgaat atgtgaaatc gaatccagag gctgcaaata aaattattaa taaaaaaaat gaaactattt ttgaaaggnt tgatagaaat tcaattgtgg tcaataagaa aaatgtatct gtttcatcac atagaaaaag nanttctgaa tatagatcat taataanata agtgannana nngnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncn tnnnnntnnn nnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc ggttattcaa ttcaattgat cgagccagtg attcattcat aactgaggaa aatgatatcg caagagaggc attagaaggt cttattactc gtttggagag tgatatcatt gatggtagtt ggatgacaaa aggttatatg gatatagatt ttaaaatgat gcctgctctt gtaaagcagg caaacaataa atatccagag atgaatctta aacttgctat gacatttgaa gatctttgcg tatcagtaca aaaaactatc gatgatggga ttcaatcttc aaggtttata acgaatatac tgaataggag tatgccccat tttgtggtaa ttgacttaaa aaccatcgat gacaagactt ccttggtatt atttgaggct acaaaacttg atcataagat ttcaaagata cttatatcga gaatgaaaag agtcattgaa gcttcccaat tgccttcttg ttactattcc attgtggaaa tggatattca gagaagtttc tctgagtgtg gaatttttag tttggctctt gctaaaaagc tgtatcatga ggcagaaaag ttggaaagaa tacacaaaga taatattgac ggtgttttat gtgattgcga gtcatattta ccttatgata agttggatac atatcttcca gttacttttt acaaacatgc acaaggtatc agtcgtatta atgaatatgt gaaatcgaat ccagaggctg caaataaaat tattaataaa aaaaatgaaa ctatttttga aaggtttgat agaaattcaa ttgtggtcaa taagaaaaat gtatctgttt catcacatag aaaaaggatt tctgaatata gatcattaat aagatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat