SSGCID: Target Page

BaclA.17330.p

Bep3 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.p
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
BaclA.17330.p.B2.GE36580	FIC cryptic	165	395
BaclA.17330.p.B3.GE36581	FIC cryptic and truncated OB-Fold	165	462
BaclA.17330.p.B4.GE37256	BID	473	604

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.594
RefSeq:	YP_004158891.1
UniProt:	E6YHH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKTKKGTIM PNSKLAGYPT LTKEELQKRQ KILQSVISAY TIRNTPLHSN TLITLQGYAK GRYSLSEFNV IMDTNLKAIL NEVTDGNINS VSPDTINAHH AIRAIINLHQ NFPGIRNIET ANYCTLIIDE LQKRRTIIDS AINTHVIENI SLHPSTLIAL EEYTKGNYSV NEFDIVMDSI ETTILDNLKN VNIDSATLNT INAHHRMKAI IALNEESLPE KFDSSYLKYL HGRLFREQFE PTGHISDHPL TSKDGITASM LTVKILNSNN VFTEDKKIQE SLQKFDQMLV AKNNLKGLSR KEFIDEAAKL FSSLSRIHPF KEGNEITQRM FFEKLAEAAG HQLDFSVVTK ERMVRAYSDT IEKKDNVRSE VIEHLFEDIS NPEKVSILKE LIYNEFNIKC KDLNNTNIIM PRDDIPYLGI YKGSIGNAIM VDTENSCVVC LKDYLTPKKL KGLKIGDRLI FKTLNKKNLD TILIPEEQLP PLTEDEIVQE LVKNTIVQEK LEKIERYSKT VYKDSKILHE HIIMMNKNPE NYRFFSDQIV SDPKSISDAA NKKFRSNTDQ KDIYKLGNAI DDYIMTVKHR KSLMVEQHNA QQKRLGTIVT MPSKAMQDVL NLPKNMQKET LKSSPLLYKE LNNFISDVTF RLLPEEHKAL CNADYKTLSE SIGISESKAK TIIKLVTQSK ELCESLKPVR PEQPTEMNIN KTNSIRM

NT Sequence

atgaaaaaaa cgaaaaaagg aacaattatg ccaaattcaa aattagctgg ttatccaact ctaacaaaag aagaattaca aaagcgccaa aaaatccttc agtcggtcat tagtgcctat actattagaa atactccttt gcactctaat acactcatca ctttacaagg gtacgcaaag ggacgttatt cattaagtga atttaatgtt atcatggaca ctaatttaaa agctattctg aatgaggtaa cagatggaaa cataaacagt gtgtctcctg atactataaa tgcacatcat gcaataagag caattattaa tctacaccaa aattttccag gtattcgaaa tatagagaca gctaattatt gtactctaat aatagatgaa ttacaaaaac gtcgcacaat tattgattca gccattaata cccatgttat tgaaaatatt tctttgcatc ctagtacact catagcttta gaagaatata caaagggaaa ctattcagta aatgaatttg atattgttat ggatagtatt gaaacaacga ttctggataa tttaaagaat gtgaatatag atagtgcgac tcttaatact ataaatgcgc atcatagaat gaaagcaatc attgccttaa acgaagagtc tttgccagaa aagtttgatt cttcttattt aaaatatctt catggacgtt tatttaggga acagtttgaa ccgactggac atattagcga tcatccactg acatctaaag atggtataac tgcaagcatg ctaacagtga aaatactgaa ctcaaataat gtgtttacag aagataaaaa aatccaagaa agcttacaga aatttgatca aatgcttgtt gcaaagaata atttaaaagg cttatcgcgc aaagaattta ttgatgaggc agcaaaactt ttttcttctc tcagccgtat acatccattt aaagaaggca acgaaattac acaacgaatg ttttttgaaa agcttgcaga agctgcaggt catcaacttg atttttcagt tgtgacaaaa gaacgcatgg tacgtgccta cagtgatacg atagagaaaa aagataatgt aaggagtgaa gttatagaac atctttttga ggatatttcc aatccagaaa aagtaagtat tttaaaggaa cttatctata acgaatttaa tattaagtgc aaagatctaa ataatacaaa tattataatg ccacgtgatg atatacccta tttaggtatt tataaaggaa gtattggaaa tgctattatg gttgatacag agaattcttg tgtcgtatgc cttaaagatt atctcacacc gaaaaaactc aaaggattaa agattggtga taggctcatt tttaaaactt taaacaaaaa aaatttagat acaattctca tcccagaaga acaattacct cctttgacgg aagatgaaat tgttcaagag cttgtaaaaa ataccattgt ccaagaaaaa ttagaaaaga tcgagcgata ttcaaaaact gtgtataaag attcaaaaat actgcatgaa catataataa tgatgaataa aaatccagaa aattatagat ttttttccga tcagattgtt tctgatccaa aaagtatttc tgatgcagca aataaaaaat tcagatctaa cacagatcaa aaggacattt ataaacttgg taacgcaatt gatgattata ttatgactgt aaaacatcgt aaaagtctaa tggttgaaca gcataatgca caacaaaaac gccttggaac aatagtaaca atgcctagca aagcaatgca agatgttctt aacttgccaa aaaatatgca gaaagaaact ttaaagtcct ctcctctcct ttacaaagaa ctcaataatt ttataagtga tgttaccttt cgtcttttac cagaagagca taaagcgctc tgtaatgctg attataaaac actctctgaa agcattggta tatcagaaag taaagcgaag acaattataa aacttgttac ccaatccaaa gagttgtgtg aatcattgaa accagtcaga cctgagcagc caacagaaat gaatataaac aaaacaaatt ctataagaat gtag

Details for BaclA.17330.p.B2.GE36580

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK GNYSVNEFDI VMDSIETTIL DNLKNVNIDS ATLNTINAHH RMKAIIALNE ESLPEKFDSS YLKYLHGRLF REQFEPTGHI SDHPLTSKDG ITASMLTVKI LNSNNVFTED KKIQESLQKF DQMLVAKNNL KGLSRKEFID EAAKLFSSLS RIHPFKEGNE ITQRMFFEKL AEAAGHQLDF SVVTKERMVR AYSDTIEKKD NVRSEVIEHL FEDISNPEKV SILKELIYNE

Validated NT Sequence

ttgnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgaaggga aactattcag taaatgaatt tgatattgtt atggatagta ttgaaacaac gattctggat aatttaaaga atgtgaatat agatagtgcg actcttaata ctataaatgc gcatcataga atgaaagcaa tcattgcctt aaacgaagag tctttgccag aaaagtttga ttcttcttat ttaaaatatc ttcatggacg tttatttagg gaacagtttg aaccgactgg acatattagc gatcatccac tgacatctaa agatggtata actgcaagca tgctaacagt gaaaatactg aactcaaata atgtgtttac agaagataaa aaaatccaag aaagcttaca gaaatttgat caaatgcttg ttgcaaagaa taatttaaaa ggcttatcgc gcaaagaatt tattgatgag gcagcaaaac ttttttcttc tctcagccgt atacatccat ttaaagaagg caacgaaatt acacaacgaa tgttttttga aaagcttgca gaagctgcag gtcatcaact tgatttttca gttgtgacaa aagaacgcat ggtacgtgcc tacagtgata cgatagagaa aaaagataat gtaaggagtg aagttataga acatcttttt gaggatattt ccaatccaga aaaagtaagt attttaaagg aacttatcta taacgaataa gtgagtaaga taggatccgg ntnnnnnnnn ccnnnnnnnn nnnannnntn nnnnnnnnnn nnnnnnnnnn nnnggnnnnt ntnntnntnt nnttatncnn nnnnnnnann ttnnnncncn nncnnnnnnn nntnnnnnnt ttannttnnt ttnnntnnnn cnnnncancn nccnnnnnnn nnnnnnncnn nnnnnnnnnn nnncnnnntn nnnncgtnna c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg gaaactattc agtaaatgaa tttgatattg ttatggatag tattgaaaca acgattctgg ataatttaaa gaatgtgaat atagatagtg cgactcttaa tactataaat gcgcatcata gaatgaaagc aatcattgcc ttaaacgaag agtctttgcc agaaaagttt gattcttctt atttaaaata tcttcatgga cgtttattta gggaacagtt tgaaccgact ggacatatta gcgatcatcc actgacatct aaagatggta taactgcaag catgctaaca gtgaaaatac tgaactcaaa taatgtgttt acagaagata aaaaaatcca agaaagctta cagaaatttg atcaaatgct tgttgcaaag aataatttaa aaggcttatc gcgcaaagaa tttattgatg aggcagcaaa acttttttct tctctcagcc gtatacatcc atttaaagaa ggcaacgaaa ttacacaacg aatgtttttt gaaaagcttg cagaagctgc aggtcatcaa cttgattttt cagttgtgac aaaagaacgc atggtacgtg cctacagtga tacgatagag aaaaaagata atgtaaggag tgaagttata gaacatcttt ttgaggatat ttccaatcca gaaaaagtaa gtattttaaa ggaacttatc tataacgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaclA.17330.p.B3.GE36581

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	96

Validated AA Sequence

Validated NT Sequence

anattttgnt tactttaaga aggaganata ccatggctca ccaccaccac caccatatga agggaaacta ttcagtaaat gaatttgata ttgttatgga tagtattgaa acaacgattc tggataattt aaagaatgtg aatatagata gtgcgactct taatactata aatgcgcatc atagaatgaa agcaatcatt gccttaaacg aagagtcttt gccagaaaag tttgattctt cttatttaaa atatcttcat ggacgtttat ttagggaaca gtttgaaccg actggacata ttagcgatca tccactgaca tctaaagatg gtataactgc aagcatgcta acagtgaaaa tactgaactc aaataatgtg tttacagaag ataaaaaaat ccaagaaagc ttacagaaat ttgatcaaat gcttgttgca aagaataatt taaaaggctt atcgcgcaaa gaatttattg atgaggcagc aaaacttttt tcttctctca gccgtataca tccatttaaa gaaggcaacg aaattacaca acgaatgttt tttgaaaagc ttgcagaagc tgcaggtcat caacttgatt tttcagttgt gacaaaagaa cgcatggtac gtgcctacag tgatacgata gagaaaaaag ataatgtaag gagtgaagtt atagaacatc tttttgagga tatttccaat ccagaaaaag taagtatttt aaaggaactt atctataacg aatttaatat taagtgcaaa gatctaaata atacaaatat tataatgcca cgtgatgata taccctattt aggtatttat aaaggaagta ttggaaatgc tattatggtt gatacagaga attcttgtgt cgtatgcctt aaagantatc tcacaccgaa aaactcaaag gantaaagan tggtgatagg ntcattttta aataagtgag nanannnnnn nnnnnnnnnn cccccccnnn nnnnnnnnnn nnnnnnnnna anaaaaaann nnaannnnnn nncnnnnnnn cgnnnnnnnn nntntntctc ntnnnntatt nncccctnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg gaaactattc agtaaatgaa tttgatattg ttatggatag tattgaaaca acgattctgg ataatttaaa gaatgtgaat atagatagtg cgactcttaa tactataaat gcgcatcata gaatgaaagc aatcattgcc ttaaacgaag agtctttgcc agaaaagttt gattcttctt atttaaaata tcttcatgga cgtttattta gggaacagtt tgaaccgact ggacatatta gcgatcatcc actgacatct aaagatggta taactgcaag catgctaaca gtgaaaatac tgaactcaaa taatgtgttt acagaagata aaaaaatcca agaaagctta cagaaatttg atcaaatgct tgttgcaaag aataatttaa aaggcttatc gcgcaaagaa tttattgatg aggcagcaaa acttttttct tctctcagcc gtatacatcc atttaaagaa ggcaacgaaa ttacacaacg aatgtttttt gaaaagcttg cagaagctgc aggtcatcaa cttgattttt cagttgtgac aaaagaacgc atggtacgtg cctacagtga tacgatagag aaaaaagata atgtaaggag tgaagttata gaacatcttt ttgaggatat ttccaatcca gaaaaagtaa gtattttaaa ggaacttatc tataacgaat ttaatattaa gtgcaaagat ctaaataata caaatattat aatgccacgt gatgatatac cctatttagg tatttataaa ggaagtattg gaaatgctat tatggttgat acagagaatt cttgtgtcgt atgccttaaa gattatctca caccgaaaaa actcaaagga ttaaagattg gtgataggct catttttaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for BaclA.17330.p.B4.GE37256

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	16kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IPEEQLPPLT EDEIVQELVK NTIVQEKLEK IERYSKTVYK DSKILHEHII MMNKNPENYR FFSDQIVSDP KSISDAANKK FRSNTDQKDI YKLGNAIDDY IMTVKHRKSL MVEQHNAQQK RLGTIVTMPS K

Validated NT Sequence

cnnnanattt tnnttacttt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgctcatcc cagaagaaca attacctcct ttgacggaag atgaaattgt tcaagagctt gtaaaaaata ccattgtcca agaaaaatta gaaaagatcg agcgatattc aaaaactgtg tataaagatt caaaaatact gcatgaacat ataataatga tgaataaaaa tccagaaaat tatagatttt tttccgatca gattgtttct gatccaaaaa gtatttctga tgcagcaaat aaaaaattca gatctaacac agatcaaaag gacatttata aacttggtaa cgcaattgat gattatatta tgactgtaaa acatcgtaaa agtctaatgg ttgaacagca taatgcacaa caaaaacgcc ttggaacaat agtaacaatg cctagcaaat aagtgagtaa gataggatcc ggctgctaac annnncnnaa ngannnnnnn nnnnnnnnnn nnnnnnnnnn nntnancgnn ngnnntcnnc nacnnnncnc naccnntacn nanacgnnan gnnggacgna nacnnnnang cnnnnntgnn cnncngcnnn nnnnncnann cnnancnntn annnnnnncn nnnnnnanat cnnnannnnn nnnnnnnnnn ccacctnnnn gannnnnana gcannnnnnn cnnnnnnncc cnncnnngtg gnngancaaa ccnngnnnnn ncnttcgann tatc

Expected Protein Sequence

MAHHHHHHLI PEEQLPPLTE DEIVQELVKN TIVQEKLEKI ERYSKTVYKD SKILHEHIIM MNKNPENYRF FSDQIVSDPK SISDAANKKF RSNTDQKDIY KLGNAIDDYI MTVKHRKSLM VEQHNAQQKR LGTIVTMPSK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcatcc cagaagaaca attacctcct ttgacggaag atgaaattgt tcaagagctt gtaaaaaata ccattgtcca agaaaaatta gaaaagatcg agcgatattc aaaaactgtg tataaagatt caaaaatact gcatgaacat ataataatga tgaataaaaa tccagaaaat tatagatttt tttccgatca gattgtttct gatccaaaaa gtatttctga tgcagcaaat aaaaaattca gatctaacac agatcaaaag gacatttata aacttggtaa cgcaattgat gattatatta tgactgtaaa acatcgtaaa agtctaatgg ttgaacagca taatgcacaa caaaaacgcc ttggaacaat agtaacaatg cctagcaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat