SSGCID: Target Page

BaclA.17330.n

Bep2 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.n
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.n.B2.GE36576	FIC partial		22	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.915
RefSeq:	YP_004159215.1
UniProt:	E6YIF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKESKRVSIT VTLQQPDLFS PYNYVYPNSN VLKNKYGITD FKSLESRCAH DSAKAVVNLM KEPCPERFDS TYLKYLHKRL FENTFEWAGH TRDVPFAFSD GTSAVVLKMK KSNSNMYFAV GKKIQQNLAK IDRILVEKNN LQGLSREEFV YHAAELFASI NYTHPFREGN GRTQRMFFLK LAEAAGHTLD FSVVTTKRME YVSVKAMQDR NLEPMQHMFE DISNPEKRRI LKQFMSDMNE VDLLKLGCQI VMVPKEDVTY TGYCKSFGSD FITIEMQDVC IVCNKDYFTP EQLRALKLDD KLTFTIAKTA NLENIFIPEE KIATLTEDEI IEKIVKHVSV QAQREKIKHY SKLVYGDSKR LEETIDMINA EPSFAKMFAK QIIDSSESII KFSNIGISIE DPAYNQNKHR GSGIQKVEEN LYKLGNAIED YGNVVEASKN AILKEHQEKQ KRCKQAVKMP SQVVQRILNL PENMWQEALK SVALLSFNKD LCNFMAKVNS RLSPCECKAL NDDNYEHFAQ SIGTSVSKAK TVIRTIKKIK EMQRQLQVAR MKRLNAMNMT C

NT Sequence

atgaaagagt caaagcgagt aagtataacg gtaacgctac aacaacctga cctattctca ccttacaatt acgtctatcc taatagtaat gtgctgaaaa ataaatatgg aatcacggat tttaaaagtt tggagtcgag atgtgcccat gattcagcaa aagcagtcgt taatttgatg aaggaacctt gtccagaacg gtttgattct acctatctaa aatatttgca taaacgctta tttgagaata cgtttgaatg ggctggacac actcgtgatg ttccatttgc attttcagat ggcacaagtg ccgtggtgtt aaagatgaaa aagagcaatt ccaatatgta tttcgcagtt ggtaaaaaga ttcagcaaaa cttagcaaag atagaccgaa tacttgtcga gaagaataat ttacaaggtt tatcgcgtga agaatttgtc tatcatgcag cagaattatt tgcttctatc aattatacac atccttttag agaaggtaat ggacgcacac aacgaatgtt ttttttaaaa cttgccgaag ctgcaggtca tacgcttgac ttctctgtcg tgacaacaaa acgtatggag tatgtgagtg ttaaggcaat gcaagataga aatttagagc ctatgcaaca tatgtttgaa gatatttcca atccagagaa gcggcgtatt ttaaagcaat tcatgagcga tatgaatgag gttgatcttt taaagttggg atgtcagatc gttatggtac caaaagaaga tgtgacctat acggggtatt gtaaaagctt tggttcagat ttcattacaa tagaaatgca agatgtttgc atcgtatgca ataaagatta ttttacacca gaacaattaa gggcattaaa gcttgatgac aagctcactt ttaccattgc aaaaactgca aatcttgaaa acatttttat cccagaggaa aaaatagcta ctttgacgga agatgaaatt attgaaaaga ttgtaaaaca tgtctctgtt caagcacaac gggagaaaat taaacattat tcaaaacttg tttatggcga ttcaaaaagg ttggaagaaa ctatagacat gattaatgca gagccgagtt ttgctaaaat gtttgctaaa caaattatag attcttctga atctattatt aaattttcga atatcggaat aagcatagag gatccggcat acaatcagaa taagcataga ggatccggca tacaaaaagt tgaggagaat ctttataaac ttggtaatgc aattgaagat tatgggaatg ttgtagaagc ttctaaaaat gcaattttaa aggagcatca agaaaaacaa aaacgttgta aacaagcggt aaaaatgccc agccaggtag ttcagcgcat tcttaatttg ccagaaaata tgtggcaaga agctttaaaa tcagtagctc ttttgtcttt taataaagat ctctgtaatt ttatggctaa ggttaattct cgtctttctc catgtgaatg taaagcactc aatgatgata actatgagca tttcgctcaa agtattggta catcagtaag taaagcaaaa actgttataa gaactattaa gaaaatcaaa gaaatgcaga gacaattgca agtggccaga atgaaacggt taaatgcaat gaatatgact tgttaa

Details for BaclA.17330.n.B2.GE36576

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY NYVYPNSNVL KNKYGITDFK SLESRCAHDS AKAVVNLMKE PCPERFDSTY LKYLHKRLFE NTFEWAGHTR DVPFAFSDGT SAVVLKMKKS NSNMYFAVGK KIQQNLAKID RILVEKNNLQ GLSREEFVYH AAELFASINY THPFREGNGR TQRMFFLKLA EAAGHTLDFS VVTTKRMEYV SVKAMQDRNL EPMQHMFEDI SNPEKRRILK QFMSDMNEVD LLKLGCQ

Validated NT Sequence

tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgtacaa ttacgtctat cctaatagta atgtgctgaa aaataaatat ggaatcacgg attttaaaag tttggagtcg agatgtgccc atgattcagc aaaagcagtc gttaatttga tgaaggaacc ttgtccagaa cggtttgatt ctacctatct aaaatatttg cataaacgct tatttgagaa tacgtttgaa tgggctggac acactcgtga tgttccattt gcattttcag atggcacaag tgccgtggtg ttaaagatga aaaagagcaa ttccaatatg tatttcgcag ttggtaaaaa gattcagcaa aacttagcaa agatagaccg aatacttgtc gagaagaata atttacaagg tttatcgcgt gaagaatttg tctatcatgc agcagaatta tttgcttcta tcaattatac acatcctttt agagaaggta atggacgcac acaacgaatg ttttttttaa aacttgccga agctgcaggt catacgcttg acttctctgt cgtgacaaca aaacgtatgg agtatgtgag tgttaaggca atgcaagata gaaatttaga gcctatgcaa catatgtttg aagatatttc caatccagag aagcggcgta ttttaaagca attcatgagc gatatgaatg aggttgatct tttaaagttg ggatgtcagt aagtgagtaa gataggatcc ggctgctana nnncccccnn nnannnnnnn ccnnnnnann nnnnnnnnnn nnnnnnnnnn nnnnnnnctn ctnnnnnnnn nnnnnnctnn cnnnnnnnnn cnnannnann ncgnnnnnnn acnntnnnnn nnncnnnnnn nnncnnnnnt nnnannantc nnnnnnncnn nnnnnnnnnn gcnncncnnc nnnnnnttgn nggtnnccnn nnnnnnnnct nnnnnnannn nntgnctnnn ntnntnnnnc tntttnnnnn nnnnnttntn ntncnncnnn nntcctnnnn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtaca attacgtcta tcctaatagt aatgtgctga aaaataaata tggaatcacg gattttaaaa gtttggagtc gagatgtgcc catgattcag caaaagcagt cgttaatttg atgaaggaac cttgtccaga acggtttgat tctacctatc taaaatattt gcataaacgc ttatttgaga atacgtttga atgggctgga cacactcgtg atgttccatt tgcattttca gatggcacaa gtgccgtggt gttaaagatg aaaaagagca attccaatat gtatttcgca gttggtaaaa agattcagca aaacttagca aagatagacc gaatacttgt cgagaagaat aatttacaag gtttatcgcg tgaagaattt gtctatcatg cagcagaatt atttgcttct atcaattata cacatccttt tagagaaggt aatggacgca cacaacgaat gtttttttta aaacttgccg aagctgcagg tcatacgctt gacttctctg tcgtgacaac aaaacgtatg gagtatgtga gtgttaaggc aatgcaagat agaaatttag agcctatgca acatatgttt gaagatattt ccaatccaga gaagcggcgt attttaaagc aattcatgag cgatatgaat gaggttgatc ttttaaagtt gggatgtcag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat