BaclA.17330.m

Bep7 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaclA.17330.m
ORGANISM: Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaclA.17330.m.B1.GE35922 full length 1 550
BaclA.17330.m.B2.GE36574 FIC 16 236
BaclA.17330.m.B3.GE36575 FIC and truncated OB-Fold 16 303
BaclA.17330.m.B4.GE37254 BID 314 451
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_171090
PATRIC ID: fig|696125.3.peg.604
RefSeq: YP_004158901.1
UniProt: E6YHI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKRLQNALR RNKNSSCDYT YSGSNTLKNK HGIQDSLYLD KKSQLLSEKI AVQLRQEPLP KNFDSSYLKY LHKCLFEEMF EWAGHTRDLP FTFADGTTAE MVNVTIPNSD VFFQESQKIA KSLQKFDQAL IEKKQLKDLS REKFVEEAVK LFSFLNYVHP FRDGNGRAQR MFFEKLAEAA GHQLDFSIAT KQRMTFACRD AIPVKGNVNY KTMQHLFEDI SNPKKVHILR NFLCSLPQYE RNYMDSHVVI VPRDNVIYTG RYKNSSSDSI IIVTKDSYIV CCKDHLTPEK LKTLKCGDTV TFAIPGNKDL DQILIPAEKL APLKKEELLE KIRNDTAIQN CEKEIKHLSK LVYGNSQKLN RSIEFIKQTA EDNEKTSNWL LKHLSTGDDL AGLTICGIKT PKRRTAERNI LPLNQKIRTY ISIVESSKNK IMQNHHLEQK RLENSVEMPS KAVQDILVMP QDIRRQKLTS KNCIELQKEL CHFLCKVNSR LSKDQSKAIR KGNYKEIAES IGMSESKAQT LIVIFNETRE VYQQLQKIKM NRSTQMAMAI
NT Sequence
atggtaaaaa gactacagaa tgctttaaga agaaataaaa attcatcttg tgattatact tattctggca gtaacacatt aaaaaataaa catggaatcc aagattctct gtatcttgac aaaaaaagtc agttgctttc agagaaaata gctgttcagt tacgtcaaga gccattacca aaaaactttg attcttccta tttgaaatat cttcataaat gcttatttga ggaaatgttt gaatgggctg gacatacccg cgatcttcca tttacatttg cagatggcac aactgccgaa atggtaaatg taacaatacc aaactcagat gttttttttc aagagagtca aaaaattgca aaaagcttac agaaatttga tcaagctctc attgagaaga aacaattaaa agatttatcg cgtgaaaaat ttgtcgagga ggcagtaaag cttttttctt ttctcaatta tgtacatcca ttcagagatg gtaatggacg tgcacaacga atgttttttg aaaagcttgc agaagctgca ggtcatcaac ttgatttttc aattgctaca aaacaacgca tgacatttgc ctgtagagat gcaatacctg taaaaggtaa tgtaaattat aaaacgatgc aacatctttt cgaagatatc tctaatccaa aaaaagtgca tattttaagg aattttttat gctctttgcc tcaatatgaa cgtaattata tggatagtca cgttgttata gtaccacgtg ataatgtaat ctatacgggt agatataaaa atagtagttc agattctatt ataattgtaa caaaagattc atatattgta tgctgtaaag atcatttaac accagaaaaa ctgaagacat taaaatgcgg tgatacagtt acctttgcaa ttccaggaaa taaagattta gatcaaattc tcattccagc agaaaaatta gctcctttaa agaaagaaga gttactcgaa aagattagaa acgatactgc gattcaaaac tgcgaaaaag aaatcaaaca tttatcaaaa cttgtctatg gcaattcaca aaagttaaat cgcagtatag agtttattaa gcaaacagca gaagataatg aaaaaacctc taactggctt ttaaagcatc tttctaccgg cgatgatctt gcgggtttaa caatatgcgg cataaaaact ccaaaacgca gaacagctga aagaaatatt ttaccactca atcaaaaaat tcgaacatat atttctattg tagaatcttc taaaaataaa attatgcaaa atcatcattt agaacaaaaa cggcttgaaa attcagtaga aatgcctagc aaagcagtgc aggatattct tgttatgcca caggatataa ggagacaaaa gttaacctca aagaattgta ttgaacttca aaaagagctt tgtcattttt tgtgtaaagt caattctcgt ctttcaaaag accagagtaa agcaatcaga aaaggtaatt ataaagaaat cgctgaaagc attggtatgt cagaaagtaa agcacaaact cttatagtaa tttttaatga aacaagagaa gtataccaac aacttcaaaa aatcaaaatg aataggtcaa cacagatggc tatggctatc taa
Details for BaclA.17330.m.B1.GE35922
HARVESTED ON: 8/28/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 64kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMV KRLQNALRRN KNSSCDYTYS GSNTLKNKHG IQDSLYLDKK SQLLSEKIAV QLRQEPLPKN FDSSYLKYLH KCLFEEMFEW AGHTRDLPFT FADGTTAEMV NVTIPNSDVF FQESQKIAKS LQKFDQALIE KKQLKDLSRE KFVEEAVKLF SFLNYVHPFR DGNGRAQRMF FEKLAEAAGH QLDFSIATKQ RMTFACRDAI PVKGNVNYKT MQHLFEDISN PKKVHILRNF LCSLPQYERN YMDSHVVIVP RDNVIYTGRY KNSSSDSIII VTXDSYIVCC KDHLTPEKLX XLKCGXXX
Validated NT Sequence
atggctcacc accaccacca ccatatggta aaaagactac agaatgcttt aagaagaaat aaaaattcat cttgtgatta tacttattct ggcagtaaca cattaaaaaa taaacatgga atccaagatt ctctgtatct tgacaaaaaa agtcagttgc tttcagagaa aatagctgtt cagttacgtc aagagccatt accaaaaaac tttgattctt cctatttgaa atatcttcat aaatgcttat ttgaggaaat gtttgaatgg gctggacata cccgcgatct tccatttaca tttgcagatg gcacaactgc cgaaatggta aatgtaacaa taccaaactc agatgttttt tttcaagaga gtcaaaaaat tgcaaaaagc ttacagaaat ttgatcaagc tctcattgag aagaaacaat taaaagattt atcgcgtgaa aaatttgtcg aggaggcagt aaagcttttt tcttttctca attatgtaca tccattcaga gatggtaatg gacgtgcaca acgaatgttt tttgaaaagc ttgcagaagc tgcaggtcat caacttgatt tttcaattgc tacaaaacaa cgcatgacat ttgcctgtag agatgcaata cctgtaaaag gtaatgtaaa ttataaaacg atgcaacatc ttttcgaaga tatctctaat ccaaaaaaag tgcatatttt aaggaatttt ttatgctctt tgcctcaata tgaacgtaat tatatggata gtcacgttgt tatagtacca cgtgataatg taatctatac gggtagatat aaaaatagta gttcagattc tattataatt gtaacaaang attcatatat tgtatgctgt aaagatcatt taacaccaga aaaactgnnn nnnttaaaat gcggtgnnnn nnttac
Expected Protein Sequence
MAHHHHHHMV KRLQNALRRN KNSSCDYTYS GSNTLKNKHG IQDSLYLDKK SQLLSEKIAV QLRQEPLPKN FDSSYLKYLH KCLFEEMFEW AGHTRDLPFT FADGTTAEMV NVTIPNSDVF FQESQKIAKS LQKFDQALIE KKQLKDLSRE KFVEEAVKLF SFLNYVHPFR DGNGRAQRMF FEKLAEAAGH QLDFSIATKQ RMTFACRDAI PVKGNVNYKT MQHLFEDISN PKKVHILRNF LCSLPQYERN YMDSHVVIVP RDNVIYTGRY KNSSSDSIII VTKDSYIVCC KDHLTPEKLK TLKCGDTVTF AIPGNKDLDQ ILIPAEKLAP LKKEELLEKI RNDTAIQNCE KEIKHLSKLV YGNSQKLNRS IEFIKQTAED NEKTSNWLLK HLSTGDDLAG LTICGIKTPK RRTAERNILP LNQKIRTYIS IVESSKNKIM QNHHLEQKRL ENSVEMPSKA VQDILVMPQD IRRQKLTSKN CIELQKELCH FLCKVNSRLS KDQSKAIRKG NYKEIAESIG MSESKAQTLI VIFNETREVY QQLQKIKMNR STQMAMAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtaa aaagactaca gaatgcttta agaagaaata aaaattcatc ttgtgattat acttattctg gcagtaacac attaaaaaat aaacatggaa tccaagattc tctgtatctt gacaaaaaaa gtcagttgct ttcagagaaa atagctgttc agttacgtca agagccatta ccaaaaaact ttgattcttc ctatttgaaa tatcttcata aatgcttatt tgaggaaatg tttgaatggg ctggacatac ccgcgatctt ccatttacat ttgcagatgg cacaactgcc gaaatggtaa atgtaacaat accaaactca gatgtttttt ttcaagagag tcaaaaaatt gcaaaaagct tacagaaatt tgatcaagct ctcattgaga agaaacaatt aaaagattta tcgcgtgaaa aatttgtcga ggaggcagta aagctttttt cttttctcaa ttatgtacat ccattcagag atggtaatgg acgtgcacaa cgaatgtttt ttgaaaagct tgcagaagct gcaggtcatc aacttgattt ttcaattgct acaaaacaac gcatgacatt tgcctgtaga gatgcaatac ctgtaaaagg taatgtaaat tataaaacga tgcaacatct tttcgaagat atctctaatc caaaaaaagt gcatatttta aggaattttt tatgctcttt gcctcaatat gaacgtaatt atatggatag tcacgttgtt atagtaccac gtgataatgt aatctatacg ggtagatata aaaatagtag ttcagattct attataattg taacaaaaga ttcatatatt gtatgctgta aagatcattt aacaccagaa aaactgaaga cattaaaatg cggtgataca gttacctttg caattccagg aaataaagat ttagatcaaa ttctcattcc agcagaaaaa ttagctcctt taaagaaaga agagttactc gaaaagatta gaaacgatac tgcgattcaa aactgcgaaa aagaaatcaa acatttatca aaacttgtct atggcaattc acaaaagtta aatcgcagta tagagtttat taagcaaaca gcagaagata atgaaaaaac ctctaactgg cttttaaagc atctttctac cggcgatgat cttgcgggtt taacaatatg cggcataaaa actccaaaac gcagaacagc tgaaagaaat attttaccac tcaatcaaaa aattcgaaca tatatttcta ttgtagaatc ttctaaaaat aaaattatgc aaaatcatca tttagaacaa aaacggcttg aaaattcagt agaaatgcct agcaaagcag tgcaggatat tcttgttatg ccacaggata taaggagaca aaagttaacc tcaaagaatt gtattgaact tcaaaaagag ctttgtcatt ttttgtgtaa agtcaattct cgtctttcaa aagaccagag taaagcaatc agaaaaggta attataaaga aatcgctgaa agcattggta tgtcagaaag taaagcacaa actcttatag taatttttaa tgaaacaaga gaagtatacc aacaacttca aaaaatcaaa atgaataggt caacacagat ggctatggct atctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BaclA.17330.m.B2.GE36574
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 27kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS CDYTYSGSNT LKNKHGIQDS LYLDKKSQLL SEKIAVQLRQ EPLPKNFDSS YLKYLHKCLF EEMFEWAGHT RDLPFTFADG TTAEMVNVTI PNSDVFFQES QKIAKSLQKF DQALIEKKQL KDLSREKFVE EAVKLFSFLN YVHPFRDGNG RAQRMFFEKL AEAAGHQLDF SIATKQRMTF ACRDAIPVKG NVNYKTMQHL FEDISNPKKV HILRNFLCSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt gtgattatac ttattctggc agtaacacat taaaaaataa acatggaatc caagattctc tgtatcttga caaaaaaagt cagttgcttt cagagaaaat agctgttcag ttacgtcaag agccattacc aaaaaacttt gattcttcct atttgaaata tcttcataaa tgcttatttg aggaaatgtt tgaatgggct ggacataccc gcgatcttcc atttacattt gcagatggca caactgccga aatggtaaat gtaacaatac caaactcaga tgtttttttt caagagagtc aaaaaattgc aaaaagctta cagaaatttg atcaagctct cattgagaag aaacaattaa aagatttatc gcgtgaaaaa tttgtcgagg aggcagtaaa gcttttttct tttctcaatt atgtacatcc attcagagat ggtaatggac gtgcacaacg aatgtttttt gaaaagcttg cagaagctgc aggtcatcaa cttgattttt caattgctac aaaacaacgc atgacatttg cctgtagaga tgcaatacct gtaaaaggta atgtaaatta taaaacgatg caacatcttt tcgaagatat ctctaatcca aaaaaagtgc atattttaag gaatttttta tgctctttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaclA.17330.m.B3.GE36575
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS CDYTYSGSNT LKNKHGIQDS LYLDKKSQLL SEKIAVQLRQ EPLPKNFDSS YLKYLHKCLF EEMFEWAGHT RDLPFTFADG TTAEMVNVTI PNSDVFFQES QKIAKSLQKF DQALIEKKQL KDLSREKFVE EAVKLFSFLN YVHPFRDGNG RAQRMFFEKL AEAAGHQLDF SIATKQRMTF ACRDAIPVKG NVNYKTMQHL FEDISNPKKV HILRNFLCSL PQYERNYMDS HVVIVPRDNV IYTGRYKNSS SDSIIIVTKD SYIVCCKDHL TPEKLKTLKC GXTVTFA
Validated NT Sequence
ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgtcttgt gattatactt attctggcag taacacatta aaaaataaac atggaatcca agattctctg tatcttgaca aaaaaagtca gttgctttca gagaaaatag ctgttcagtt acgtcaagag ccattaccaa aaaactttga ttcttcctat ttgaaatatc ttcataaatg cttatttgag gaaatgtttg aatgggctgg acatacccgc gatcttccat ttacatttgc agatggcaca actgccgaaa tggtaaatgt aacaatacca aactcagatg ttttttttca agagagtcaa aaaattgcaa aaagcttaca gaaatttgat caagctctca ttgagaagaa acaattaaaa gatttatcgc gtgaaaaatt tgtcgaggag gcagtaaagc ttttttcttt tctcaattat gtacatccat tcagagatgg taatggacgt gcacaacgaa tgttttttga aaagcttgca gaagctgcag gtcatcaact tgatttttca attgctacaa aacaacgcat gacatttgcc tgtagagatg caatacctgt aaaaggtaat gtaaattata aaacgatgca acatcttttc gaagatatct ctaatccaaa aaaagtgcat attttaagga attttttatg ctctttgcct caatatgaac gtaattatat ggatagtcac gttgttatag taccacgtga taatgtaatc tatacgggta gatataaaaa tagtagttca gattctatta taattgtaac aaaagattca tatattgtat gctgtaaaga tcatttaaca ccagaaaaac tgaagacatt aaaatgcggt ganacagtta cctttgcata agtgagtaaa naggancnnn nnnnnnnnnn ccc
Expected Protein Sequence
MAHHHHHHMS CDYTYSGSNT LKNKHGIQDS LYLDKKSQLL SEKIAVQLRQ EPLPKNFDSS YLKYLHKCLF EEMFEWAGHT RDLPFTFADG TTAEMVNVTI PNSDVFFQES QKIAKSLQKF DQALIEKKQL KDLSREKFVE EAVKLFSFLN YVHPFRDGNG RAQRMFFEKL AEAAGHQLDF SIATKQRMTF ACRDAIPVKG NVNYKTMQHL FEDISNPKKV HILRNFLCSL PQYERNYMDS HVVIVPRDNV IYTGRYKNSS SDSIIIVTKD SYIVCCKDHL TPEKLKTLKC GDTVTFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt gtgattatac ttattctggc agtaacacat taaaaaataa acatggaatc caagattctc tgtatcttga caaaaaaagt cagttgcttt cagagaaaat agctgttcag ttacgtcaag agccattacc aaaaaacttt gattcttcct atttgaaata tcttcataaa tgcttatttg aggaaatgtt tgaatgggct ggacataccc gcgatcttcc atttacattt gcagatggca caactgccga aatggtaaat gtaacaatac caaactcaga tgtttttttt caagagagtc aaaaaattgc aaaaagctta cagaaatttg atcaagctct cattgagaag aaacaattaa aagatttatc gcgtgaaaaa tttgtcgagg aggcagtaaa gcttttttct tttctcaatt atgtacatcc attcagagat ggtaatggac gtgcacaacg aatgtttttt gaaaagcttg cagaagctgc aggtcatcaa cttgattttt caattgctac aaaacaacgc atgacatttg cctgtagaga tgcaatacct gtaaaaggta atgtaaatta taaaacgatg caacatcttt tcgaagatat ctctaatcca aaaaaagtgc atattttaag gaatttttta tgctctttgc ctcaatatga acgtaattat atggatagtc acgttgttat agtaccacgt gataatgtaa tctatacggg tagatataaa aatagtagtt cagattctat tataattgta acaaaagatt catatattgt atgctgtaaa gatcatttaa caccagaaaa actgaagaca ttaaaatgcg gtgatacagt tacctttgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaclA.17330.m.B4.GE37254
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLI PAEKLAPLKK EELLEKIRND TAIQNCEKEI KHLSKLVYGN SQKLNRSIEF IKQTAEDNEK TSNWLLKHLS TGDDLAGLTI CGIKTPKRRT AERNILPLNQ KIRTYISIVE SSKNKIMQNH HLEQKRLENS VEMPSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcattc cagcagaaaa attagctcct ttaaagaaag aagagttact cgaaaagatt agaaacgata ctgcgattca aaactgcgaa aaagaaatca aacatttatc aaaacttgtc tatggcaatt cacaaaagtt aaatcgcagt atagagttta ttaagcaaac agcagaagat aatgaaaaaa cctctaactg gcttttaaag catctttcta ccggcgatga tcttgcgggt ttaacaatat gcggcataaa aactccaaaa cgcagaacag ctgaaagaaa tattttacca ctcaatcaaa aaattcgaac atatatttct attgtagaat cttctaaaaa taaaattatg caaaatcatc atttagaaca aaaacggctt gaaaattcag tagaaatgcc tagcaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at