SSGCID: Target Page

BaclA.17330.k

Bep9 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.k
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.k.B1.GE35623	full length		1	367
BaclA.17330.k.B2.GE37252	BID		128	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.986
RefSeq:	YP_004159285.1
UniProt:	E6YIM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTHNTPATN SEIYENSEIY DNPATDSGIY DTPATNSEIY DNPATDSGIY DTPATNSEIY GNPATDSGIY DTPATNSEIY ENSEIYGNPA IDSGIYDTPA TNSEIYENSE IYGNSATDSE IYENYDTYKK NKELVSLTKK ELIIKIANDP SVQEQATKIR NLSQIVFGKS EILNNEIEQI NNHPSLGKVF HKQVLESYKS FSKLAGRKIF FIKTQERKTA EENIPILSQA IKEYMEVVKH SKDKILQNYQ KERKSFILIQ NNFSKEVTDI LNLPRNMWHK TLEQNNNINV NKELSNFLNQ VNSCLSKDEL AMLRNNEYKL LAKNTNIPEN NAKDFILIFK KAQSLQNEIN RLNQSSPQKM RTMVITH

NT Sequence

atgaaaaccc ataacactcc tgcaacaaat tctgaaatct atgaaaattc tgaaatctat gacaatcctg caacagactc tggaatctat gacactcctg cgacaaattc tgaaatctat gacaatcctg caacagactc tggaatctat gacactcctg cgacaaattc tgaaatctat ggcaatcctg caacagactc tggaatctat gacactcctg cgacaaattc tgaaatctat gaaaattctg aaatctatgg caatcctgca atagactctg gaatctatga cactcctgcg acaaattctg aaatctatga aaattctgaa atctatggca attctgcaac agactctgaa atctatgaga actatgacac atataaaaag aataaagaat tagtttcttt aaccaaaaaa gaactcatca taaagattgc caatgatcct tctgttcaag aacaagcaac aaaaattagg aatttgtcac aaattgtatt tggaaagtcg gaaatattaa acaacgagat agaacagatt aataaccatc caagtttagg gaaagtattt cataaacaag tgctagaatc ttataagtct ttttctaaac ttgcaggtcg taaaatattt ttcataaaaa ctcaggaacg taagacagct gaagaaaata ttccaatact tagtcaagct attaaagaat atatggaagt tgtaaaacat tctaaggaca aaattcttca aaattatcaa aaagagcgaa aatcctttat ccttattcaa aataatttta gcaaagaagt gacagatatt cttaatctac cacgtaatat gtggcacaaa actttagaac agaataacaa tattaatgtt aacaaagagc tttcaaattt tttaaatcaa gttaattctt gtctttcaaa agatgaattg gcaatgctta ggaataatga gtataaacta cttgctaaaa atacgaatat accagaaaac aatgcaaaag atttcatact gatctttaaa aaagctcaaa gcttgcagaa cgaaattaat agattaaacc aatcttctcc tcagaaaatg agaacgatgg ttataacaca ttaa

Details for BaclA.17330.k.B1.GE35623

HARVESTED ON:	7/10/2012
SEQUENCED ON:	2/22/2016
EXPECTED MW:	42kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	84

Validated AA Sequence

MAHHHHHHMK THNTPATNSE IYENSEIYDN PATDSGIYDT PATNSEIYDN PATDSGIYDT PATNSEIYGN PATDSGIYDT PATNSEIYEN SEIYGNPAID SGIYDTPATN SEIYENSEIY GNSATDSEIY ENYDTYKKNK ELVSLTKKEL IIKIANDPSV QEQATKIRNL SQIVFGKSEI LNNEIEQINN HPSLGKVFHK QVLESYKSFS KLAGRKIFFI KTQERKTAEE NIPILSQAIK EYMEVVKHSK DKILQNYQKE RKSFILIQNN FSKEVTDILN LPRNMWHKTL EQNNNINVNK ELSNFLNXVN SCLSXM

Validated NT Sequence

atggctcacc accaccacca ccatatgaaa acccataaca ctcctgcaac aaattctgaa atctatgaaa attctgaaat ctatgacaat cctgcaacag actctggaat ctatgacact cctgcgacaa attctgaaat ctatgacaat cctgcaacag actctggaat ctatgacact cctgcgacaa attctgaaat ctatggcaat cctgcaacag actctggaat ctatgacact cctgcgacaa attctgaaat ctatgaaaat tctgaaatct atggcaatcc tgcaatagac tctggaatct atgacactcc tgcgacaaat tctgaaatct atgaaaattc tgaaatctat ggcaattctg caacagactc tgaaatctat gagaactatg acacatataa aaagaataaa gaattagttt ctttaaccaa aaaagaactc atcataaaga ttgccaatga tccttctgtt caagaacaag caacaaaaat taggaatttg tcacaaattg tatttggaaa gtcggaaata ttaaacaacg agatagaaca gattaataac catccaagtt tagggaaagt atttcataaa caagtgctag aatcttataa gtctttttct aaacttgcag gtcgtaaaat atttttcata aaaactcagg aacgtaagac agctgaagaa aatattccaa tacttagtca agctattaaa gaatatatgg aagttgtaaa acattctaag gacaaaattc ttcaaaatta tcaaaaagag cgaaaatcct ttatccttat tcaaaataat tttagcaaag aagtgacaga tattcttaat ctaccacgta atatgtggca caaaacttta gaacagaata acaatattaa tgttaacaaa gagctttcaa attttttaaa tcnagtnaat tcttgtcttt caaanatga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa cccataacac tcctgcaaca aattctgaaa tctatgaaaa ttctgaaatc tatgacaatc ctgcaacaga ctctggaatc tatgacactc ctgcgacaaa ttctgaaatc tatgacaatc ctgcaacaga ctctggaatc tatgacactc ctgcgacaaa ttctgaaatc tatggcaatc ctgcaacaga ctctggaatc tatgacactc ctgcgacaaa ttctgaaatc tatgaaaatt ctgaaatcta tggcaatcct gcaatagact ctggaatcta tgacactcct gcgacaaatt ctgaaatcta tgaaaattct gaaatctatg gcaattctgc aacagactct gaaatctatg agaactatga cacatataaa aagaataaag aattagtttc tttaaccaaa aaagaactca tcataaagat tgccaatgat ccttctgttc aagaacaagc aacaaaaatt aggaatttgt cacaaattgt atttggaaag tcggaaatat taaacaacga gatagaacag attaataacc atccaagttt agggaaagta tttcataaac aagtgctaga atcttataag tctttttcta aacttgcagg tcgtaaaata tttttcataa aaactcagga acgtaagaca gctgaagaaa atattccaat acttagtcaa gctattaaag aatatatgga agttgtaaaa cattctaagg acaaaattct tcaaaattat caaaaagagc gaaaatcctt tatccttatt caaaataatt ttagcaaaga agtgacagat attcttaatc taccacgtaa tatgtggcac aaaactttag aacagaataa caatattaat gttaacaaag agctttcaaa ttttttaaat caagttaatt cttgtctttc aaaagatgaa ttggcaatgc ttaggaataa tgagtataaa ctacttgcta aaaatacgaa tataccagaa aacaatgcaa aagatttcat actgatcttt aaaaaagctc aaagcttgca gaacgaaatt aatagattaa accaatcttc tcctcagaaa atgagaacga tggttataac acattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BaclA.17330.k.B2.GE37252

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY KKNKELVSLT KKELIIKIAN DPSVQEQATK IRNLSQIVFG KSEILNNEIE QINNHPSLGK VFHKQVLESY KSFSKLAGRK IFFIKTQERK TAEENIPILS QAIKEYMEVV KHSKDKILQN YQKERKSFIL IQNNFSKE

Validated NT Sequence

cnnnnnattt tgtnnnnntt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgtataaaa agaataaaga attagtttct ttaaccaaaa aagaactcat cataaagatt gccaatgatc cttctgttca agaacaagca acaaaaatta ggaatttgtc acaaattgta tttggaaagt cggaaatatt aaacaacgag atagaacaga ttaataacca tccaagttta gggaaagtat ttcataaaca agtgctagaa tcttataagt ctttttctaa acttgcaggt cgtaaaatat ttttcataaa aactcaggaa cgtaagacag ctgaagaaaa tattccaata cttagtcaag ctattaaaga atatatggaa gttgtaaaac attctaagga caaaattctt caaaattatc aaaaagagcg aaaatccttt atccttattc aaaataattt tagcaaagaa taagtgagta agataggatc cggctgctaa caannnnnnn aangaannnn nnnnnnanaa aannnnngan ttnanngnnc nnnnnnnnnn ntgnnnngan gaaaanaann aacaacaant aattnnnnnn nnnnatgnnn tnnnnngtgn nactgccnng acnnntnnan ngaacnagcn nnngtcatat ccccnntnnn nngnngngnn nncntnnnnn tnnnnnnncn nnnngannac nannngnnnn nnangtgnnn tnnnnanann nnnnnnnnnn nnnnctcnng gnnnnnnnnn nntcnnnnnt ncancnnnna nnnnnanccc cnncangggg tnantnnnta nnatnnnatc ccnnnnnnnn nnnnncnnnc nnnannnncc

Expected Protein Sequence

MAHHHHHHYK KNKELVSLTK KELIIKIAND PSVQEQATKI RNLSQIVFGK SEILNNEIEQ INNHPSLGKV FHKQVLESYK SFSKLAGRKI FFIKTQERKT AEENIPILSQ AIKEYMEVVK HSKDKILQNY QKERKSFILI QNNFSKE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattataaaa agaataaaga attagtttct ttaaccaaaa aagaactcat cataaagatt gccaatgatc cttctgttca agaacaagca acaaaaatta ggaatttgtc acaaattgta tttggaaagt cggaaatatt aaacaacgag atagaacaga ttaataacca tccaagttta gggaaagtat ttcataaaca agtgctagaa tcttataagt ctttttctaa acttgcaggt cgtaaaatat ttttcataaa aactcaggaa cgtaagacag ctgaagaaaa tattccaata cttagtcaag ctattaaaga atatatggaa gttgtaaaac attctaagga caaaattctt caaaattatc aaaaagagcg aaaatccttt atccttattc aaaataattt tagcaaagaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat