BaclA.17330.j

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaclA.17330.j
ORGANISM: Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.j.B1.GE35622 full length 1 640
BaclA.17330.j.B2.GE36570 FIC 90 325
BaclA.17330.j.B3.GE36571 FIC and truncated OB-Fold 90 395
BaclA.17330.j.B4.GE37251 BID 406 543
BaclA.17330.j.B5.GE37428 N-term extended FIC 1 325
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.j.B1.PW37228 full length 1 640
BaclA.17330.j.B4.PW37440 BID 406 543
BaclA.17330.j.B4.PW37441 BID 406 543 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|696125.3.peg.236
RefSeq: YP_004158516.1
UniProt: E6YGD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKDSTPNSI SFLQKQPQKF HEAFDAAISL YMLDGLFISY ETLEIVRKYA RKGYSLEQFN NLMDNAGLKN NDVRLNARKV KINSNTLQSS NGYAYNNSRT LKNKYGMEGK AFERICAHDI IQAIVNLKEE PLPEKFDSSY LKYLHKCLFE KTFEGAGCIR EPPFKFSDYL KYLHKCLFEK MFEWAGFTHE LPFKFSDGTA LINNEIKDGL KRIDQMLAEK NNLRGLSREE FIHEASTIFA LLNNLYPFQA GSECTQRIFF EKMAEAAGHK LDFSVVTEKR MRFACHAALP SDGNDIGDIT PMHHLFEDIS NPEKVRILKE FMIHMRDTNV IKYVEMKNKI FVLPNEGSTY TGIYEDCSPD FIMFMVKDNY FVCKKDYFAP ETLKKLRFGK ELTFTASMRD NFENILIPGE KLAPLTEKKI VARIVKNASV QTSREKIERL SKRVYGDSKI LNKDISLINI DSRLGKQHVK QIVDFSKYTS KLAGTVILRI RSPKRRRAQA NYFKLVQEVD SYISTVIATR EKILQEYQKE QERCQQVVEM PSQALQDIIN LPKEDRIKAL NDNPSLNKDI EDLLNKVKIR LSPREHQALD GNHYEKLARS VGISMSKAQK IAEIVQKVQI ARQLTRKYKI HKSRTMGMTR
NT Sequence
atggaaaaag attcaactcc taattctata tcttttttgc aaaaacaacc acaaaaattc cacgaagcgt ttgatgcggc catcagtttg tatatgcttg atgggttatt tatttcttat gaaacactcg aaattgtgag aaaatatgcg cgcaagggtt attcactcga acaatttaat aatttgatgg ataatgctgg actaaagaat aatgatgtga gattaaatgc gcggaaagta aagataaaca gtaatacctt acagtcctct aatggttatg cttataataa tagccgaacg ctgaagaata aatatggaat ggaaggtaag gcttttgaga ggatatgtgc acatgatata atacaagcga tcgttaatct aaaagaagaa ccgttgccgg aaaagtttga ttcctcttat ctaaaatatc ttcataaatg tctgtttgag aagacgtttg aaggggctgg ttgtatccgc gagcctccat ttaaattttc agattatcta aaatatcttc ataaatgttt gtttgagaag atgtttgaat gggctggttt tacccacgag cttccattta aattttcaga tggcacagca ctgatcaata atgaaattaa agacggttta aaaagaattg atcaaatgct tgccgagaag aataatttgc gaggtctatc acgtgaagaa tttattcatg aagcatcaac aatatttgcc ttgctcaata atttatatcc ttttcaggct ggaagtgaat gcacacaacg aatatttttt gaaaaaatgg ccgaggctgc gggacataag cttgatttct cagttgtgac agaaaaacgc atgcgatttg cttgtcatgc tgcattacca agtgatggca acgatatagg tgatataact cctatgcacc atctttttga agatatttcc aatccagaaa aagtacgtat tttaaaggaa tttatgatac acatgcgaga tacgaatgtt atcaaatacg tagagatgaa aaataagatt tttgtcctgc caaatgaagg cagtacctat acaggaatct atgaagattg tagcccagat ttcattatgt ttatggtgaa ggataattac tttgtatgca aaaaagatta ttttgcgcca gaaacattaa agaaattacg gtttggtaaa gagcttactt ttacagcttc aatgcgagac aattttgaga acatactgat cccaggagaa aaattagctc ctttgacgga aaaaaaaatt gtcgcaagaa ttgtaaaaaa tgcttctgtc caaacgagtc gagaaaaaat cgagcgttta tcaaaacgtg tctatgggga ctcgaaaata ttgaacaaag atataagtct gattaatata gattcacgtt tgggtaaaca gcatgtcaaa cagattgtgg atttttctaa atatacttct aagcttgcag gtacagtaat attgcgtata agaagtccaa aacgcagaag agctcaggcg aattacttta agcttgttca ggaagttgat agttacataa gtactgtaat cgctactaga gaaaaaattc ttcaagaata tcaaaaagaa caagaacgtt gccaacaagt agtagaaatg cctagccagg cattgcaaga tattattaat ttgccaaaag aagatcgaat aaaagcgtta aatgacaatc cttcgctgaa caaagatatc gaagatcttt tgaataaagt caaaattcgt ctttcgccaa gagagcatca agcacttgat gggaatcatt atgagaaact cgctaggagc gttggcatat caatgagtaa ggcacaaaaa attgcagaaa ttgttcagaa agttcagata gcaagacagt taaccagaaa atacaaaatt cataagtcga gaacgatggg tatgactcgt taa
Details for BaclA.17330.j.B1.GE35622
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 75kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME KDSTPNSISF LQKQPQKFHE AFDAAISLYM LDGLFISYET LEIVRKYARK GYSLEQFNNL MDNAGLKNND VRLNARKVKI NSNTLQSSNG YAYNNSRTLK NKYGMEGKAF ERICAHDIIQ AIVNLKEEPL PEKFDSSYLK YLHKCLFEKT FEGAGCIREP PFKFSDYLKY LHKCLFEKMF EWAGFTHELP FKFSDGTALI NNEIKDGLKR IDQMLAEKNN LRGLSREEFI HEASTIFALL NNLYPFQAGS ECTQRIFFEK MAEAAGHKLD FSVVTEKRMR FACHAALPSD GNDIGDITPM HHLFEDISNP EKVRILKEFM IHMRDTNVIK YVEMKNKIFV LPNEGSTYTG IYEDCSPDFI MFMVKDNYFV CKKDYFAPET LKKLRFGKEL TFTASMRDNF ENILIPGEKL APLTEKKIVA RIVKNASVQT SREKIERLSK RVYGDSKILN KDISLINIDS RLGKQHVKQI VDFSKYTSKL AGTVILRIRS PKRRRAQANY FKLVQEVDSY ISTVIATREK ILQEYQKEQE RCQQVVEMPS QALQDIINLP KEDRIKALND NPSLNKDIED LLNKVKIRLS PREHQALDGN HYEKLARSVG ISMSKAQKIA EIVQKVQIAR QLTRKYKIHK SRTMGMTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aagattcaac tcctaattct atatcttttt tgcaaaaaca accacaaaaa ttccacgaag cgtttgatgc ggccatcagt ttgtatatgc ttgatgggtt atttatttct tatgaaacac tcgaaattgt gagaaaatat gcgcgcaagg gttattcact cgaacaattt aataatttga tggataatgc tggactaaag aataatgatg tgagattaaa tgcgcggaaa gtaaagataa acagtaatac cttacagtcc tctaatggtt atgcttataa taatagccga acgctgaaga ataaatatgg aatggaaggt aaggcttttg agaggatatg tgcacatgat ataatacaag cgatcgttaa tctaaaagaa gaaccgttgc cggaaaagtt tgattcctct tatctaaaat atcttcataa atgtctgttt gagaagacgt ttgaaggggc tggttgtatc cgcgagcctc catttaaatt ttcagattat ctaaaatatc ttcataaatg tttgtttgag aagatgtttg aatgggctgg ttttacccac gagcttccat ttaaattttc agatggcaca gcactgatca ataatgaaat taaagacggt ttaaaaagaa ttgatcaaat gcttgccgag aagaataatt tgcgaggtct atcacgtgaa gaatttattc atgaagcatc aacaatattt gccttgctca ataatttata tccttttcag gctggaagtg aatgcacaca acgaatattt tttgaaaaaa tggccgaggc tgcgggacat aagcttgatt tctcagttgt gacagaaaaa cgcatgcgat ttgcttgtca tgctgcatta ccaagtgatg gcaacgatat aggtgatata actcctatgc accatctttt tgaagatatt tccaatccag aaaaagtacg tattttaaag gaatttatga tacacatgcg agatacgaat gttatcaaat acgtagagat gaaaaataag atttttgtcc tgccaaatga aggcagtacc tatacaggaa tctatgaaga ttgtagccca gatttcatta tgtttatggt gaaggataat tactttgtat gcaaaaaaga ttattttgcg ccagaaacat taaagaaatt acggtttggt aaagagctta cttttacagc ttcaatgcga gacaattttg agaacatact gatcccagga gaaaaattag ctcctttgac ggaaaaaaaa attgtcgcaa gaattgtaaa aaatgcttct gtccaaacga gtcgagaaaa aatcgagcgt ttatcaaaac gtgtctatgg ggactcgaaa atattgaaca aagatataag tctgattaat atagattcac gtttgggtaa acagcatgtc aaacagattg tggatttttc taaatatact tctaagcttg caggtacagt aatattgcgt ataagaagtc caaaacgcag aagagctcag gcgaattact ttaagcttgt tcaggaagtt gatagttaca taagtactgt aatcgctact agagaaaaaa ttcttcaaga atatcaaaaa gaacaagaac gttgccaaca agtagtagaa atgcctagcc aggcattgca agatattatt aatttgccaa aagaagatcg aataaaagcg ttaaatgaca atccttcgct gaacaaagat atcgaagatc ttttgaataa agtcaaaatt cgtctttcgc caagagagca tcaagcactt gatgggaatc attatgagaa actcgctagg agcgttggca tatcaatgag taaggcacaa aaaattgcag aaattgttca gaaagttcag atagcaagac agttaaccag aaaatacaaa attcataagt cgagaacgat gggtatgact cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BaclA.17330.j.B2.GE36570
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 28kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS NGYAYNNSRT LKNKYGMEGK AFERICAHDI IQAIVNLKEE PLPEKFDSSY LKYLHKCLFE KTFEGAGCIR EPPFKFSDYL KYLHKCLFEK MFEWAGFTHE LPFKFSDGTA LINNEIKDGL KRIDQMLAEK NNLRGLSREE FIHEASTIFA LLNNLYPFQA GSECTQRIFF EKMAEAAGHK LDFSVVTEKR MRFACHAALP SDGNDIGDIT PMHHLFEDIS NPEKVRILKE FMIHM
Validated NT Sequence
ttntttactt tangaaggag anataccatg gctcaccacc accaccacca tatgtctaat ggttatgctt ataataatag ccgaacgctg aagaataaat atggaatgga aggtaaggct tttgagagga tatgtgcaca tgatataata caagcgatcg ttaatctaaa agaagaaccg ttgccggaaa agtttgattc ctcttatcta aaatatcttc ataaatgtct gtttgagaag acgtttgaag gggctggttg tatccgcgag cctccattta aattttcaga ttatctaaaa tatcttcata aatgtttgtt tgagaagatg tttgaatggg ctggttttac ccacgagctt ccatttaaat tttcagatgg cacagcactg atcaataatg aaattaaaga cggtttaaaa agaattgatc aaatgcttgc cgagaagaat aatttgcgag gtctatcacg tgaagaattt attcatgaag catcaacaat atttgccttg ctcaataatt tatatccttt tcaggctgga agtgaatgca cacaacgaat attttttgaa aaaatggccg aggctgcggg acataagctt gatttctcag ttgtgacaga aaaacgcatg cgatttgctt gtcatgctgc attaccaagt gatggcaacg atataggtga tataactcct atgcaccatc tttttgaaga tatttccaat ccagaaaaag tacgtatttt aaaggaattt atgatacaca tgtaagtgag taagatagga tccggctgcn nnnnnccccc nnnnnnnnnn nnnnnnnnna nnaaaannnt nnnnnnncnn nnnnnnnnnn nnnnnannnn nnnnnnnnnn nnnnncnnnn nnnnnnnnng nntnaaannn nnngntnnnn cnnccncnnn nnncgtnncn nnnnnnnnnn nnnnnnnnnn nacncncnnc nnnnnnnnnt nncnnnnnnc nnnc
Expected Protein Sequence
MAHHHHHHMS NGYAYNNSRT LKNKYGMEGK AFERICAHDI IQAIVNLKEE PLPEKFDSSY LKYLHKCLFE KTFEGAGCIR EPPFKFSDYL KYLHKCLFEK MFEWAGFTHE LPFKFSDGTA LINNEIKDGL KRIDQMLAEK NNLRGLSREE FIHEASTIFA LLNNLYPFQA GSECTQRIFF EKMAEAAGHK LDFSVVTEKR MRFACHAALP SDGNDIGDIT PMHHLFEDIS NPEKVRILKE FMIHM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta atggttatgc ttataataat agccgaacgc tgaagaataa atatggaatg gaaggtaagg cttttgagag gatatgtgca catgatataa tacaagcgat cgttaatcta aaagaagaac cgttgccgga aaagtttgat tcctcttatc taaaatatct tcataaatgt ctgtttgaga agacgtttga aggggctggt tgtatccgcg agcctccatt taaattttca gattatctaa aatatcttca taaatgtttg tttgagaaga tgtttgaatg ggctggtttt acccacgagc ttccatttaa attttcagat ggcacagcac tgatcaataa tgaaattaaa gacggtttaa aaagaattga tcaaatgctt gccgagaaga ataatttgcg aggtctatca cgtgaagaat ttattcatga agcatcaaca atatttgcct tgctcaataa tttatatcct tttcaggctg gaagtgaatg cacacaacga atattttttg aaaaaatggc cgaggctgcg ggacataagc ttgatttctc agttgtgaca gaaaaacgca tgcgatttgc ttgtcatgct gcattaccaa gtgatggcaa cgatataggt gatataactc ctatgcacca tctttttgaa gatatttcca atccagaaaa agtacgtatt ttaaaggaat ttatgataca catgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaclA.17330.j.B3.GE36571
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMS NGYAYNNSRT LKNKYGMEGK AFERICAHDI IQAIVNLKEE PLPEKFDSSY LKYLHKCLFE KTFEGAGCIR EPPFKFSDYL KYLHKCLFEK MFEWAGFTHE LPFKFSDGTA LINNEIKDGL KRIDQMLAEK NNLRGLSREE FIHEASTIFA LLNNLYPFQA GSECTQRIFF EKMAEAAGHK LDFSVVTEKR MRFACHAALP SDGNDIGDIT PMHHLFEDIS NPEKVRILKE FMIHMRDTNV IKYVEMKNKI FVLPNEGSTY TGIYEXCSPD FIMFMVKXNY FVCKKIILRX KH
Validated NT Sequence
ttgnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgtctaat ggttatgctt ataataatag ccgaacgctg aagaataaat atggaatgga aggtaaggct tttgagagga tatgtgcaca tgatataata caagcgatcg ttaatctaaa agaagaaccg ttgccggaaa agtttgattc ctcttatcta aaatatcttc ataaatgtct gtttgagaag acgtttgaag gggctggttg tatccgcgag cctccattta aattttcaga ttatctaaaa tatcttcata aatgtttgtt tgagaagatg tttgaatggg ctggttttac ccacgagctt ccatttaaat tttcagatgg cacagcactg atcaataatg aaattaaaga cggtttaaaa agaattgatc aaatgcttgc cgagaagaat aatttgcgag gtctatcacg tgaagaattt attcatgaag catcaacaat atttgccttg ctcaataatt tatatccttt tcaggctgga agtgaatgca cacaacgaat attttttgaa aaaatggccg aggctgcggg acataagctt gatttctcag ttgtgacaga aaaacgcatg cgatttgctt gtcatgctgc attaccaagt gatggcaacg atataggtga tataactcct atgcaccatc tttttgaaga tatttccaat ccagaaaaag tacgtatttt aaaggaattt atgatacaca tgcgagatac gaatgttatc aaatacgtag agatgaaaaa taagattttt gtcctgccna atgaaggcag tacctataca ggaatctatg aagantgtag cccagatttc attatgttta tggtgaagga naattacttt gtatgcaaaa agattatttt gcgccngaaa cattaaagaa attangnttt ggtaaagagc ttacttttac ntnagtgagn ananagnanc nnnnnnnnnn ncc
Expected Protein Sequence
MAHHHHHHMS NGYAYNNSRT LKNKYGMEGK AFERICAHDI IQAIVNLKEE PLPEKFDSSY LKYLHKCLFE KTFEGAGCIR EPPFKFSDYL KYLHKCLFEK MFEWAGFTHE LPFKFSDGTA LINNEIKDGL KRIDQMLAEK NNLRGLSREE FIHEASTIFA LLNNLYPFQA GSECTQRIFF EKMAEAAGHK LDFSVVTEKR MRFACHAALP SDGNDIGDIT PMHHLFEDIS NPEKVRILKE FMIHMRDTNV IKYVEMKNKI FVLPNEGSTY TGIYEDCSPD FIMFMVKDNY FVCKKDYFAP ETLKKLRFGK ELTFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta atggttatgc ttataataat agccgaacgc tgaagaataa atatggaatg gaaggtaagg cttttgagag gatatgtgca catgatataa tacaagcgat cgttaatcta aaagaagaac cgttgccgga aaagtttgat tcctcttatc taaaatatct tcataaatgt ctgtttgaga agacgtttga aggggctggt tgtatccgcg agcctccatt taaattttca gattatctaa aatatcttca taaatgtttg tttgagaaga tgtttgaatg ggctggtttt acccacgagc ttccatttaa attttcagat ggcacagcac tgatcaataa tgaaattaaa gacggtttaa aaagaattga tcaaatgctt gccgagaaga ataatttgcg aggtctatca cgtgaagaat ttattcatga agcatcaaca atatttgcct tgctcaataa tttatatcct tttcaggctg gaagtgaatg cacacaacga atattttttg aaaaaatggc cgaggctgcg ggacataagc ttgatttctc agttgtgaca gaaaaacgca tgcgatttgc ttgtcatgct gcattaccaa gtgatggcaa cgatataggt gatataactc ctatgcacca tctttttgaa gatatttcca atccagaaaa agtacgtatt ttaaaggaat ttatgataca catgcgagat acgaatgtta tcaaatacgt agagatgaaa aataagattt ttgtcctgcc aaatgaaggc agtacctata caggaatcta tgaagattgt agcccagatt tcattatgtt tatggtgaag gataattact ttgtatgcaa aaaagattat tttgcgccag aaacattaaa gaaattacgg tttggtaaag agcttacttt tacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaclA.17330.j.B4.GE37251
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLI PGEKLAPLTE KKIVARIVKN ASVQTSREKI ERLSKRVYGD SKILNKDISL INIDSRLGKQ HVKQIVDFSK YTSKLAGTVI LRIRSPKRRR AQANYFKLVQ EVDSYISTVI ATREKILQEY QKEQERCQQV VEMPSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgatcc caggagaaaa attagctcct ttgacggaaa aaaaaattgt cgcaagaatt gtaaaaaatg cttctgtcca aacgagtcga gaaaaaatcg agcgtttatc aaaacgtgtc tatggggact cgaaaatatt gaacaaagat ataagtctga ttaatataga ttcacgtttg ggtaaacagc atgtcaaaca gattgtggat ttttctaaat atacttctaa gcttgcaggt acagtaatat tgcgtataag aagtccaaaa cgcagaagag ctcaggcgaa ttactttaag cttgttcagg aagttgatag ttacataagt actgtaatcg ctactagaga aaaaattctt caagaatatc aaaaagaaca agaacgttgc caacaagtag tagaaatgcc tagccagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaclA.17330.j.B5.GE37428
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 39kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME KDSTPNSISF LQKQPQKFHE AFDAAISLYM LDGLFISYET LEIVRKYARK GYSLEQFNNL MDNAGLKNND VRLNARKVKI NSNTLQSSNG YAYNNSRTLK NKYGMEGKAF ERICAHDIIQ AIVNLKEEPL PEKFDSSYLK YLHKCLFEKT FEGAGCIREP PFKFSDYLKY LHKCLFEKMF EWAGFTHELP FKFSDGTALI NNEIKDGLKR IDQMLAEKNN LRGLSREEFI HEASTIFALL NNLYPFQAGS ECTQRIFFEK MAXAAGHKLD FSVVTEKRMR FACHAALPSD GNDXXXITPM XHLFEXXSXX KXRIXXNL
Validated NT Sequence
attttgnnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggaa aaagattcaa ctcctaattc tatatctttt ttgcaaaaac aaccacaaaa attccacgaa gcgtttgatg cggccatcag tttgtatatg cttgatgggt tatttatttc ttatgaaaca ctcgaaattg tgagaaaata tgcgcgcaag ggttattcac tcgaacaatt taataatttg atggataatg ctggactaaa gaataatgat gtgagattaa atgcgcggaa agtaaagata aacagtaata ccttacagtc ctctaatggt tatgcttata ataatagccg aacgctgaag aataaatatg gaatggaagg taaggctttt gagaggatat gtgcacatga tataatacaa gcgatcgtta atctaaaaga agaaccgttg ccggaaaagt ttgattcctc ttatctaaaa tatcttcata aatgtctgtt tgagaagacg tttgaagggg ctggttgtat ccgcgagcct ccatttaaat tttcagatta tctaaaatat cttcataaat gtttgtttga gaagatgttt gaatgggctg gttttaccca cgagcttcca tttaaatttt cagatggcac agcactgatc aataatgaaa ttaaagacgg tttaaaaaga attgatcaaa tgcttgccga gaagaataat ttgcgaggtc tatcacgtga agaatttatt catgaagcat caacaatatt tgccttgctc aataatttat atccttttca ggctggaagt gaatgcacac aacgaatatt ttttgaaaaa atggccgang ctgcgggaca taagcttgat ttctcagttg tgacagaaaa acgcatgcga tttgcttgtc atgctgcatt accaagtgat ggcaacgata tngnnnanat aactcctatg cancatcttt ttgaagannt ttcnnncnga aaantacgta tttnannnaa tttatgannc ncatgtaagt gagnannann nnancnnnnn nnnnnnnncc
Expected Protein Sequence
MAHHHHHHME KDSTPNSISF LQKQPQKFHE AFDAAISLYM LDGLFISYET LEIVRKYARK GYSLEQFNNL MDNAGLKNND VRLNARKVKI NSNTLQSSNG YAYNNSRTLK NKYGMEGKAF ERICAHDIIQ AIVNLKEEPL PEKFDSSYLK YLHKCLFEKT FEGAGCIREP PFKFSDYLKY LHKCLFEKMF EWAGFTHELP FKFSDGTALI NNEIKDGLKR IDQMLAEKNN LRGLSREEFI HEASTIFALL NNLYPFQAGS ECTQRIFFEK MAEAAGHKLD FSVVTEKRMR FACHAALPSD GNDIGDITPM HHLFEDISNP EKVRILKEFM IHM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aagattcaac tcctaattct atatcttttt tgcaaaaaca accacaaaaa ttccacgaag cgtttgatgc ggccatcagt ttgtatatgc ttgatgggtt atttatttct tatgaaacac tcgaaattgt gagaaaatat gcgcgcaagg gttattcact cgaacaattt aataatttga tggataatgc tggactaaag aataatgatg tgagattaaa tgcgcggaaa gtaaagataa acagtaatac cttacagtcc tctaatggtt atgcttataa taatagccga acgctgaaga ataaatatgg aatggaaggt aaggcttttg agaggatatg tgcacatgat ataatacaag cgatcgttaa tctaaaagaa gaaccgttgc cggaaaagtt tgattcctct tatctaaaat atcttcataa atgtctgttt gagaagacgt ttgaaggggc tggttgtatc cgcgagcctc catttaaatt ttcagattat ctaaaatatc ttcataaatg tttgtttgag aagatgtttg aatgggctgg ttttacccac gagcttccat ttaaattttc agatggcaca gcactgatca ataatgaaat taaagacggt ttaaaaagaa ttgatcaaat gcttgccgag aagaataatt tgcgaggtct atcacgtgaa gaatttattc atgaagcatc aacaatattt gccttgctca ataatttata tccttttcag gctggaagtg aatgcacaca acgaatattt tttgaaaaaa tggccgaggc tgcgggacat aagcttgatt tctcagttgt gacagaaaaa cgcatgcgat ttgcttgtca tgctgcatta ccaagtgatg gcaacgatat aggtgatata actcctatgc accatctttt tgaagatatt tccaatccag aaaaagtacg tattttaaag gaatttatga tacacatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaclA.17330.j.B1.PW37228
PURIFICATION DATe: 12/10/2012
CONCENTRATION: 2.03mg/ml
OBSERVED MW: 65kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHME KDSTPNSISF LQKQPQKFHE AFDAAISLYM LDGLFISYET LEIVRKYARK GYSLEQFNNL MDNAGLKNND VRLNARKVKI NSNTLQSSNG YAYNNSRTLK NKYGMEGKAF ERICAHDIIQ AIVNLKEEPL PEKFDSSYLK YLHKCLFEKT FEGAGCIREP PFKFSDYLKY LHKCLFEKMF EWAGFTHELP FKFSDGTALI NNEIKDGLKR IDQMLAEKNN LRGLSREEFI HEASTIFALL NNLYPFQAGS ECTQRIFFEK MAEAAGHKLD FSVVTEKRMR FACHAALPSD GNDIGDITPM HHLFEDISNP EKVRILKEFM IHMRDTNVIK YVEMKNKIFV LPNEGSTYTG IYEDCSPDFI MFMVKDNYFV CKKDYFAPET LKKLRFGKEL TFTASMRDNF ENILIPGEKL APLTEKKIVA RIVKNASVQT SREKIERLSK RVYGDSKILN KDISLINIDS RLGKQHVKQI VDFSKYTSKL AGTVILRIRS PKRRRAQANY FKLVQEVDSY ISTVIATREK ILQEYQKEQE RCQQVVEMPS QALQDIINLP KEDRIKALND NPSLNKDIED LLNKVKIRLS PREHQALDGN HYEKLARSVG ISMSKAQKIA EIVQKVQIAR QLTRKYKIHK SRTMGMTR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA AAGATTCAAC TCCTAATTCT ATATCTTTTT TGCAAAAACA ACCACAAAAA TTCCACGAAG CGTTTGATGC GGCCATCAGT TTGTATATGC TTGATGGGTT ATTTATTTCT TATGAAACAC TCGAAATTGT GAGAAAATAT GCGCGCAAGG GTTATTCACT CGAACAATTT AATAATTTGA TGGATAATGC TGGACTAAAG AATAATGATG TGAGATTAAA TGCGCGGAAA GTAAAGATAA ACAGTAATAC CTTACAGTCC TCTAATGGTT ATGCTTATAA TAATAGCCGA ACGCTGAAGA ATAAATATGG AATGGAAGGT AAGGCTTTTG AGAGGATATG TGCACATGAT ATAATACAAG CGATCGTTAA TCTAAAAGAA GAACCGTTGC CGGAAAAGTT TGATTCCTCT TATCTAAAAT ATCTTCATAA ATGTCTGTTT GAGAAGACGT TTGAAGGGGC TGGTTGTATC CGCGAGCCTC CATTTAAATT TTCAGATTAT CTAAAATATC TTCATAAATG TTTGTTTGAG AAGATGTTTG AATGGGCTGG TTTTACCCAC GAGCTTCCAT TTAAATTTTC AGATGGCACA GCACTGATCA ATAATGAAAT TAAAGACGGT TTAAAAAGAA TTGATCAAAT GCTTGCCGAG AAGAATAATT TGCGAGGTCT ATCACGTGAA GAATTTATTC ATGAAGCATC AACAATATTT GCCTTGCTCA ATAATTTATA TCCTTTTCAG GCTGGAAGTG AATGCACACA ACGAATATTT TTTGAAAAAA TGGCCGAGGC TGCGGGACAT AAGCTTGATT TCTCAGTTGT GACAGAAAAA CGCATGCGAT TTGCTTGTCA TGCTGCATTA CCAAGTGATG GCAACGATAT AGGTGATATA ACTCCTATGC ACCATCTTTT TGAAGATATT TCCAATCCAG AAAAAGTACG TATTTTAAAG GAATTTATGA TACACATGCG AGATACGAAT GTTATCAAAT ACGTAGAGAT GAAAAATAAG ATTTTTGTCC TGCCAAATGA AGGCAGTACC TATACAGGAA TCTATGAAGA TTGTAGCCCA GATTTCATTA TGTTTATGGT GAAGGATAAT TACTTTGTAT GCAAAAAAGA TTATTTTGCG CCAGAAACAT TAAAGAAATT ACGGTTTGGT AAAGAGCTTA CTTTTACAGC TTCAATGCGA GACAATTTTG AGAACATACT GATCCCAGGA GAAAAATTAG CTCCTTTGAC GGAAAAAAAA ATTGTCGCAA GAATTGTAAA AAATGCTTCT GTCCAAACGA GTCGAGAAAA AATCGAGCGT TTATCAAAAC GTGTCTATGG GGACTCGAAA ATATTGAACA AAGATATAAG TCTGATTAAT ATAGATTCAC GTTTGGGTAA ACAGCATGTC AAACAGATTG TGGATTTTTC TAAATATACT TCTAAGCTTG CAGGTACAGT AATATTGCGT ATAAGAAGTC CAAAACGCAG AAGAGCTCAG GCGAATTACT TTAAGCTTGT TCAGGAAGTT GATAGTTACA TAAGTACTGT AATCGCTACT AGAGAAAAAA TTCTTCAAGA ATATCAAAAA GAACAAGAAC GTTGCCAACA AGTAGTAGAA ATGCCTAGCC AGGCATTGCA AGATATTATT AATTTGCCAA AAGAAGATCG AATAAAAGCG TTAAATGACA ATCCTTCGCT GAACAAAGAT ATCGAAGATC TTTTGAATAA AGTCAAAATT CGTCTTTCGC CAAGAGAGCA TCAAGCACTT GATGGGAATC ATTATGAGAA ACTCGCTAGG AGCGTTGGCA TATCAATGAG TAAGGCACAA AAAATTGCAG AAATTGTTCA GAAAGTTCAG ATAGCAAGAC AGTTAACCAG AAAATACAAA ATTCATAAGT CGAGAACGAT GGGTATGACT CGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for BaclA.17330.j.B4.PW37440
PURIFICATION DATe: 2/5/2014
CONCENTRATION: 6.4mg/ml
OBSERVED MW: 17kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLI PGEKLAPLTE KKIVARIVKN ASVQTSREKI ERLSKRVYGD SKILNKDISL INIDSRLGKQ HVKQIVDFSK YTSKLAGTVI LRIRSPKRRR AQANYFKLVQ EVDSYISTVI ATREKILQEY QKEQERCQQV VEMPSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGATCC CAGGAGAAAA ATTAGCTCCT TTGACGGAAA AAAAAATTGT CGCAAGAATT GTAAAAAATG CTTCTGTCCA AACGAGTCGA GAAAAAATCG AGCGTTTATC AAAACGTGTC TATGGGGACT CGAAAATATT GAACAAAGAT ATAAGTCTGA TTAATATAGA TTCACGTTTG GGTAAACAGC ATGTCAAACA GATTGTGGAT TTTTCTAAAT ATACTTCTAA GCTTGCAGGT ACAGTAATAT TGCGTATAAG AAGTCCAAAA CGCAGAAGAG CTCAGGCGAA TTACTTTAAG CTTGTTCAGG AAGTTGATAG TTACATAAGT ACTGTAATCG CTACTAGAGA AAAAATTCTT CAAGAATATC AAAAAGAACA AGAACGTTGC CAACAAGTAG TAGAAATGCC TAGCCAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for BaclA.17330.j.B4.PW37441
PURIFICATION DATe: 2/5/2014
CONCENTRATION: 6.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLI PGEKLAPLTE KKIVARIVKN ASVQTSREKI ERLSKRVYGD SKILNKDISL INIDSRLGKQ HVKQIVDFSK YTSKLAGTVI LRIRSPKRRR AQANYFKLVQ EVDSYISTVI ATREKILQEY QKEQERCQQV VEMPSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGATCC CAGGAGAAAA ATTAGCTCCT TTGACGGAAA AAAAAATTGT CGCAAGAATT GTAAAAAATG CTTCTGTCCA AACGAGTCGA GAAAAAATCG AGCGTTTATC AAAACGTGTC TATGGGGACT CGAAAATATT GAACAAAGAT ATAAGTCTGA TTAATATAGA TTCACGTTTG GGTAAACAGC ATGTCAAACA GATTGTGGAT TTTTCTAAAT ATACTTCTAA GCTTGCAGGT ACAGTAATAT TGCGTATAAG AAGTCCAAAA CGCAGAAGAG CTCAGGCGAA TTACTTTAAG CTTGTTCAGG AAGTTGATAG TTACATAAGT ACTGTAATCG CTACTAGAGA AAAAATTCTT CAAGAATATC AAAAAGAACA AGAACGTTGC CAACAAGTAG TAGAAATGCC TAGCCAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT