BaclA.17330.h

Bep7 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaclA.17330.h
ORGANISM: Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.h.B1.GE35620 full length 1 567
BaclA.17330.h.B3.GE36569 FIC and truncated OB-Fold 32 322
BaclA.17330.h.B4.GE37023 BID 333 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|696125.3.peg.600
RefSeq: YP_004158897.1
UniProt: E6YHH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKTLNKHFP PPTLQELNTL YFNKTEKLEA LPDNYTYSNS NTLKNKYGIK DFIRFVQKCE HDVAEAVVNL RQEALPKKFD SSYLKYLHKR LFENTFEWAG HTRDLPFKFE DGTVAVMRKI GSSSSIVSLA VGDKIKECLE KIDRTLSKKN NLQGLSREEF VNDAGEIFAF LTNVYPFREG NGLTQQIFFE KLAEAAGHKL DFSVATKERM TRIYSDAIML KGDVDNTTTV KHLFEDISNP EKVRILKEFI NYRIRVDVDS CNINDQIIVA PNEGDTYTGT YEADSPNSIM IKTMDSCVIC HKDYLTPEQL KALKFGDKIT FIAPITKNLD KILIPAEKLA PLTEKKIIET IINNIYILKN REEIEHLSKL VYGNSKILDQ NLHLIHKNPE TSKQLAEQIE NSPQSISKFA GFKIWIIKSP KRKIAEKNFS KLSQAIEKYV DTIESTKNRI LKEHQVEQRR VKQAIEMPSE AVLNILNSPK DKWNKYLESS PLLREEFNNF MSKIVLRLST SEHKALNDAD YKTLAESIGI SENRARIITE IFNKAKEICI EFRAFQINKL KIMMIEN
NT Sequence
atgaaaaaaa ctctaaataa gcattttccc cctcctacat tgcaagagct aaatacttta tacttcaata aaacagaaaa acttgaagca ttacctgata attataccta ttctaacagc aatacactaa aaaataaata tggaataaaa gactttatac gctttgttca aaaatgtgag catgatgtag cagaagcagt tgtcaattta cgtcaagaag cactaccaaa aaagtttgat tcctcctatt taaaatacct tcataaacgc ttatttgaaa atacgtttga atgggctggg catacccgcg atctcccatt taagtttgaa gatggtacag ttgccgtcat gagaaaaatc ggtagttcat cctcgatagt ttctctagca gttggtgata aaattaaaga atgtttagaa aaaatcgata gaacactttc taaaaaaaac aatttacaag gattatcgcg cgaagagttt gttaatgatg caggggaaat atttgctttt cttactaatg tatatccatt tagagaaggg aatgggctta cacaacaaat attttttgaa aagcttgcag aagctgcagg tcataagctt gatttttcag ttgcgacaaa agaacgcatg acacgtatct atagtgatgc aataatgcta aaaggtgatg tagataatac aactactgtg aagcatctgt ttgaagatat ttccaatcca gaaaaagtac gtattttaaa ggaattcatc aactacagaa taagagtaga tgttgactcc tgtaatataa atgatcaaat tattgtagca ccaaatgaag gagataccta cacgggcacc tatgaagctg atagcccaaa ttccattatg attaaaacaa tggattcttg tgttatatgc cataaagatt atctcacacc agaacaactt aaggcattaa aatttggtga taaaatcacc tttatagctc caataaccaa aaatttagac aagattctca ttccagcgga gaaattagct cctttaacgg aaaaaaaaat aatcgaaacg attataaata atatctatat tttaaaaaat cgagaagaaa tcgagcattt atcaaaactt gtctatggca actcaaaaat attagatcaa aatctgcatc ttattcataa gaatcctgaa acaagtaaac agcttgccga acagattgaa aattctcctc aatccatttc taagtttgcg ggttttaaaa tatggataat aaagagtcca aaacgcaaaa tagctgaaaa gaatttttca aaacttagtc aagcaattga aaaatatgta gatactatag aatctactaa aaatagaatt cttaaagagc atcaagtaga acaaagacgt gttaaacaag caatagaaat gcctagcgaa gcagtattaa atattcttaa ttcaccaaag gataaatgga acaaatattt agagtcttcc cctttacttc gagaagaatt caataacttt atgagtaaaa tcgttttgcg tctttcaaca agcgagcata aagcacttaa tgatgctgat tataaaacac tcgctgaaag cattggcata tcagaaaata gagcgagaat aattacagaa atttttaata aagccaaaga aatatgcatc gaatttcgag ctttccaaat aaataaattg aaaataatga tgatagaaaa ttaa
Details for BaclA.17330.h.B1.GE35620
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 66kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MRKIGSSSSI VSLAVGDKIK ECLEKIDRTL SKKNNLQGLS REEFVNDAGE IFAFLTNVYP FREGNGLTQQ IFFEKLAEAA GHKLDFSVAT KERMTRIYSD AIMLKGDVDN TTTVKHLFED ISNPEKVRIL KEFINYRIRV DVDSCNINDQ IIVAPNEGXT YTGTYEADSP NSIMIKTMDS CVICHKDYLT PEQLKALKFG DKITFIAPIT KNLDKILIPA EKLAPLTEKK IIETIINNIY ILKNREEIEH LSKLVYGNSK ILDQNLHLIH KNPETSKQLA EQIENSPQSI SKFAGFKIWI IKSPKRKIAE KNFSKLSQAI EKYVDTIEST KNRILKEHQV EQRRVKQAIE MPSEAVLNIL NSPKDKWNKY LESSPLLREE FNNFMSKIVL RLSTSEHKAL NDADYKTLAE SIGISENRAR IITEIFNKAK EICIEFRAFQ INKLKIMMIE N
Validated NT Sequence
atcttactca cttaattttc tatcatcatt attttcaatt tatttatttg gaaagctcga aattcgatgc atatttcttt ggctttatta aaaatttctg taattattct cgctctattt tctgatatgc caatgctttc agcgagtgtt ttataatcag catcattaag tgctttatgc tcgcttgttg aaagacgcaa aacgatttta ctcataaagt tattgaattc ttctcgaagt aaaggggaag actctaaata tttgttccat ttatcctttg gtgaattaag aatatttaat actgcttcgc taggcatttc tattgcttgt ttaacacgtc tttgttctac ttgatgctct ttaagaattc tatttttagt agattctata gtatctacat atttttcaat tgcttgacta agttttgaaa aattcttttc agctattttg cgttttggac tctttattat ccatatttta aaacccgcaa acttagaaat ggattgagga gaattttcaa tctgttcggc aagctgttta cttgtttcag gattcttatg aataagatgc agattttgat ctaatatttt tgagttgcca tagacaagtt ttgataaatg ctcgatttct tctcgatttt ttaaaatata gatattattt ataatcgttt cgattatttt tttttccgtt aaaggagcta atttctccgc tggaatgaga atcttgtcta aatttttggt tattggagct ataaaggtga ttttatcacc aaattttaat gccttaagtt gttctggtgt gagataatct ttatggcata taacacaaga atccattgtt ttaatcataa tggaatttgg gctatcagct tcataggtgc ccgtgtaggt ntctccttca ttnggtgcta caataatttg atcatttata ttacaggagt caacatctac tcttattctg tagttgatga attcctttaa aatacgtact ttttctggat tggaaatatc ttcaaacaga tgcttcacag tagttgtatt atctacatca ccttttagca ttattgcatc actatagata cgtgtcatgc gttcttttgt cgcaactgaa aaatcaagct tatgacctgc agcttctgca agcttttcaa aaaatatttg ttgtgtaagc ccattccctt ctctaaatgg atatacatta gtaagaaaag caaatatttc ccctgcatca ttaacaaact cttcgcgcga taatccttgt aaattgtttt ttttagaaag tgttctatcg attttttcta aacattcttt aattttatca ccaactgcta gagaaactat cgaggatgaa ctaccgattt ttctcatgac ggcaactgta ccatcttcaa acttaaatgg gagatcgcgg gtatgcccag cccattcaaa cgtattttca aataagcgtt tatgaaggta ttttaaatag gaggaatcaa acttttttgg tagtgcttct tgacgtaaat tgacaactgc ttctgctaca tcatgctcac atttttgaac aaagcgtata aagtctttta ttccatattt attttttagt gtattgctgt tagaataggt ataattatca ggtaatgctt caagtttttc tgttttattg aagtataaag tatttagctc ttgcaatgta ggagggggaa aatgcttatt tagagttt
Expected Protein Sequence
MAHHHHHHMK KTLNKHFPPP TLQELNTLYF NKTEKLEALP DNYTYSNSNT LKNKYGIKDF IRFVQKCEHD VAEAVVNLRQ EALPKKFDSS YLKYLHKRLF ENTFEWAGHT RDLPFKFEDG TVAVMRKIGS SSSIVSLAVG DKIKECLEKI DRTLSKKNNL QGLSREEFVN DAGEIFAFLT NVYPFREGNG LTQQIFFEKL AEAAGHKLDF SVATKERMTR IYSDAIMLKG DVDNTTTVKH LFEDISNPEK VRILKEFINY RIRVDVDSCN INDQIIVAPN EGDTYTGTYE ADSPNSIMIK TMDSCVICHK DYLTPEQLKA LKFGDKITFI APITKNLDKI LIPAEKLAPL TEKKIIETII NNIYILKNRE EIEHLSKLVY GNSKILDQNL HLIHKNPETS KQLAEQIENS PQSISKFAGF KIWIIKSPKR KIAEKNFSKL SQAIEKYVDT IESTKNRILK EHQVEQRRVK QAIEMPSEAV LNILNSPKDK WNKYLESSPL LREEFNNFMS KIVLRLSTSE HKALNDADYK TLAESIGISE NRARIITEIF NKAKEICIEF RAFQINKLKI MMIEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaactctaaa taagcatttt ccccctccta cattgcaaga gctaaatact ttatacttca ataaaacaga aaaacttgaa gcattacctg ataattatac ctattctaac agcaatacac taaaaaataa atatggaata aaagacttta tacgctttgt tcaaaaatgt gagcatgatg tagcagaagc agttgtcaat ttacgtcaag aagcactacc aaaaaagttt gattcctcct atttaaaata ccttcataaa cgcttatttg aaaatacgtt tgaatgggct gggcataccc gcgatctccc atttaagttt gaagatggta cagttgccgt catgagaaaa atcggtagtt catcctcgat agtttctcta gcagttggtg ataaaattaa agaatgttta gaaaaaatcg atagaacact ttctaaaaaa aacaatttac aaggattatc gcgcgaagag tttgttaatg atgcagggga aatatttgct tttcttacta atgtatatcc atttagagaa gggaatgggc ttacacaaca aatatttttt gaaaagcttg cagaagctgc aggtcataag cttgattttt cagttgcgac aaaagaacgc atgacacgta tctatagtga tgcaataatg ctaaaaggtg atgtagataa tacaactact gtgaagcatc tgtttgaaga tatttccaat ccagaaaaag tacgtatttt aaaggaattc atcaactaca gaataagagt agatgttgac tcctgtaata taaatgatca aattattgta gcaccaaatg aaggagatac ctacacgggc acctatgaag ctgatagccc aaattccatt atgattaaaa caatggattc ttgtgttata tgccataaag attatctcac accagaacaa cttaaggcat taaaatttgg tgataaaatc acctttatag ctccaataac caaaaattta gacaagattc tcattccagc ggagaaatta gctcctttaa cggaaaaaaa aataatcgaa acgattataa ataatatcta tattttaaaa aatcgagaag aaatcgagca tttatcaaaa cttgtctatg gcaactcaaa aatattagat caaaatctgc atcttattca taagaatcct gaaacaagta aacagcttgc cgaacagatt gaaaattctc ctcaatccat ttctaagttt gcgggtttta aaatatggat aataaagagt ccaaaacgca aaatagctga aaagaatttt tcaaaactta gtcaagcaat tgaaaaatat gtagatacta tagaatctac taaaaataga attcttaaag agcatcaagt agaacaaaga cgtgttaaac aagcaataga aatgcctagc gaagcagtat taaatattct taattcacca aaggataaat ggaacaaata tttagagtct tcccctttac ttcgagaaga attcaataac tttatgagta aaatcgtttt gcgtctttca acaagcgagc ataaagcact taatgatgct gattataaaa cactcgctga aagcattggc atatcagaaa atagagcgag aataattaca gaaattttta ataaagccaa agaaatatgc atcgaatttc gagctttcca aataaataaa ttgaaaataa tgatgataga aaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaclA.17330.h.B3.GE36569
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP DNYTYSNSNT LKNKYGIKDF IRFVQKCEHD VAEAVVNLRQ EALPKKFDSS YLKYLHKRLF ENTFEWAGHT RDLPFKFEDG TVAVMRKIGS SSSIVSLAVG DKIKECLEKI DRTLSKKNNL QGLSREEFVN DAGEIFAFLT NVYPFREGNG LTQQIFFEKL AEAAGHKLDF SVATKERMTR IYSDAIMLKG DVDNTTTVKH LFEDISNPEK VRILKEFINY RIRVDVDSCN INDQIIVAPN EGDTYTGTYE ADSPNSIMIK TMDSCVICHK DYLTPEQLKX LKFGDKITFI
Validated NT Sequence
anattttgnn nnntttaaga aggagatata ccatggctca ccaccaccac caccatatgc ctgataatta tacctattct aacagcaata cactaaaaaa taaatatgga ataaaagact ttatacgctt tgttcaaaaa tgtgagcatg atgtagcaga agcagttgtc aatttacgtc aagaagcact accaaaaaag tttgattcct cctatttaaa ataccttcat aaacgcttat ttgaaaatac gtttgaatgg gctgggcata cccgcgatct cccatttaag tttgaagatg gtacagttgc cgtcatgaga aaaatcggta gttcatcctc gatagtttct ctagcagttg gtgataaaat taaagaatgt ttagaaaaaa tcgatagaac actttctaaa aaaaacaatt tacaaggatt atcgcgcgaa gagtttgtta atgatgcagg ggaaatattt gcttttctta ctaatgtata tccatttaga gaagggaatg ggcttacaca acaaatattt tttgaaaagc ttgcagaagc tgcaggtcat aagcttgatt tttcagttgc gacaaaagaa cgcatgacac gtatctatag tgatgcaata atgctaaaag gtgatgtaga taatacaact actgtgaagc atctgtttga agatatttcc aatccagaaa aagtacgtat tttaaaggaa ttcatcaact acagaataag agtagatgtt gactcctgta atataaatga tcaaattatt gtagcaccaa atgaaggaga tacctacacg ggcacctatg aagctgatag cccaaattcc attatgatta aaacaatgga ttcttgtgtt atatgccata aagattatct cacaccngaa caacttaagg nattaaaatt tggtgataaa atcaccttta tataagtgag taaganagga nnnnnnnnnn nnnncccc
Expected Protein Sequence
MAHHHHHHMP DNYTYSNSNT LKNKYGIKDF IRFVQKCEHD VAEAVVNLRQ EALPKKFDSS YLKYLHKRLF ENTFEWAGHT RDLPFKFEDG TVAVMRKIGS SSSIVSLAVG DKIKECLEKI DRTLSKKNNL QGLSREEFVN DAGEIFAFLT NVYPFREGNG LTQQIFFEKL AEAAGHKLDF SVATKERMTR IYSDAIMLKG DVDNTTTVKH LFEDISNPEK VRILKEFINY RIRVDVDSCN INDQIIVAPN EGDTYTGTYE ADSPNSIMIK TMDSCVICHK DYLTPEQLKA LKFGDKITFI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg ataattatac ctattctaac agcaatacac taaaaaataa atatggaata aaagacttta tacgctttgt tcaaaaatgt gagcatgatg tagcagaagc agttgtcaat ttacgtcaag aagcactacc aaaaaagttt gattcctcct atttaaaata ccttcataaa cgcttatttg aaaatacgtt tgaatgggct gggcataccc gcgatctccc atttaagttt gaagatggta cagttgccgt catgagaaaa atcggtagtt catcctcgat agtttctcta gcagttggtg ataaaattaa agaatgttta gaaaaaatcg atagaacact ttctaaaaaa aacaatttac aaggattatc gcgcgaagag tttgttaatg atgcagggga aatatttgct tttcttacta atgtatatcc atttagagaa gggaatgggc ttacacaaca aatatttttt gaaaagcttg cagaagctgc aggtcataag cttgattttt cagttgcgac aaaagaacgc atgacacgta tctatagtga tgcaataatg ctaaaaggtg atgtagataa tacaactact gtgaagcatc tgtttgaaga tatttccaat ccagaaaaag tacgtatttt aaaggaattc atcaactaca gaataagagt agatgttgac tcctgtaata taaatgatca aattattgta gcaccaaatg aaggagatac ctacacgggc acctatgaag ctgatagccc aaattccatt atgattaaaa caatggattc ttgtgttata tgccataaag attatctcac accagaacaa cttaaggcat taaaatttgg tgataaaatc acctttatat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaclA.17330.h.B4.GE37023
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPAEKLAPLT EKKIIETIIN NIYILKNREE IEHLSKLVYG NSKILDQNLH LIHKNPETSK QLAEQIENSP QSISKFAGFK IWIIKSPKRK IAEKNFSKLS QAIEKYVDTI ESTKNRILKE HQVEQRRVKQ AIEMPSE
Validated NT Sequence
atcttactca cttattcgct aggcatttct attgcttgtt taacacgtct ttgttctact tgatgctctt taagaattct atttttagta gattctatag tatctacata tttttcaatt gcttgactaa gttttgaaaa attcttttca gctattttgc gttttggact ctttattatc catattttaa aacccgcaaa cttagaaatg gattgaggag aattttcaat ctgttcggca agctgtttac ttgtttcagg attcttatga ataagatgca gattttgatc taatattttt gagttgccat agacaagttt tgataaatgc tcgatttctt ctcgattttt taaaatatag atattattta taatcgtttc gattattttt ttttccgtta aaggagctaa tttctccgct ggaatgagca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHLI PAEKLAPLTE KKIIETIINN IYILKNREEI EHLSKLVYGN SKILDQNLHL IHKNPETSKQ LAEQIENSPQ SISKFAGFKI WIIKSPKRKI AEKNFSKLSQ AIEKYVDTIE STKNRILKEH QVEQRRVKQA IEMPSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcattc cagcggagaa attagctcct ttaacggaaa aaaaaataat cgaaacgatt ataaataata tctatatttt aaaaaatcga gaagaaatcg agcatttatc aaaacttgtc tatggcaact caaaaatatt agatcaaaat ctgcatctta ttcataagaa tcctgaaaca agtaaacagc ttgccgaaca gattgaaaat tctcctcaat ccatttctaa gtttgcgggt tttaaaatat ggataataaa gagtccaaaa cgcaaaatag ctgaaaagaa tttttcaaaa cttagtcaag caattgaaaa atatgtagat actatagaat ctactaaaaa tagaattctt aaagagcatc aagtagaaca aagacgtgtt aaacaagcaa tagaaatgcc tagcgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at