BaclA.17330.g
Bep1 protein (VirB-translocated Bartonella effector protein)
| CENTER ID: | BaclA.17330.g |
| ORGANISM: | Bartonella clarridgeiae CIP 104772 / 73 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaclA.17330.g.B1.GE35681 | full length | 1 | 558 | ||
| BaclA.17330.g.B2.GE37021 | FIC | 21 | 242 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaclA.17330.g.B1.PW36267 | full length | 1 | 558 |
Structures
4NPS
DEPOSITED: 11/22/2013
DETERMINATION: XRay
CLONE: BaclA.17330.g.B1.GE35681
PROTEIN: BaclA.17330.g.B1.PW36267
Publications by SSGCID
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
de Beer T, Dehio C, Edwards T, Harms A, Myler P, Phan I, Schirmer T, Dranow D, Tamegger S, Dietz N
Microorganisms - 2021
volume 9, issue 8, pages 1645
PMID: 34442725; PMCID: PMC8401265
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171090 |
| PATRIC ID: | fig|696125.3.peg.65 |
| RefSeq: | YP_004158348.1 |
| UniProt: | E6YFW2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKTLSNAAI ATESISNQAL PSHYNYPNSL TLKNKYGITN HKDFTNKCAH DSAKAAINLR QEEPPKKFDS SYLKYLHKQL FKETFEWAGH TRDLPFTFAD GTVGVMPEMI RSNWRTEQPI IFATGNKVQD GLKNIDKMLI EKNNLQGLSH KEFIENIAEI FACLNYTHPF REGNGRTQRI FCEKLAQAAG YYLDFSVVTK ERMSEASITA AQDSNLEPMK KLFDDISNPI KTAVLKECIN SLKNIDHKNI NDCIIVAADK GLTYKGIYTD NSPDSIILKT DNIYIICSKN DLAPEQLKTL KLGDECTITV PMKQNIENTL IPKEKLPPLT KNAIIENIKK DTLIQAKLRQ VTHLSKLVYG SSKILNNQLE LINTNQKSGE QLSAQITSSP QSISKLAGIK IFGMKNSMRK KAEDNIIKLA NSIKSYGSAV KNVETTMIQQ HKSEQKRLSK TVKLPSTKLQ NILNLPEEKQ KEVLLEEKSL ASSIGKELTD FISSLNARLS PSERKMILES NHQQFAKNVG ISESQAETII KTAQKAKEVY QKMQKPAVDL TKHLIVSG
NT Sequence
atggaaaaaa cactctcaaa tgcagcaata gcaacagaat caatatccaa ccaagcttta cctagtcatt ataactatcc taatagtctc acactgaaaa ataaatatgg aataacaaac cataaagact ttactaataa atgtgcgcat gattcagcga aagcagctat taatttgcgt caagaagaac cgcctaaaaa atttgattct tcctatttaa aatatcttca caaacaatta tttaaggaaa cgtttgaatg ggctggacat acccgtgatc ttccatttac atttgcagat ggtacagttg gcgttatgcc agaaatgata agatcaaatt ggagaacaga acaaccaatt atttttgcaa ccggcaataa agtgcaagat ggtttaaaaa atatagataa gatgcttatc gaaaagaata atttacaagg cttatcgcac aaagaattta ttgagaatat agcagaaata tttgcttgcc ttaactatac gcatcctttt agagaaggca atggacgtac acaacggata ttttgcgaaa agcttgctca agctgcaggt tattatcttg acttttcagt tgtgacaaaa gaacgtatgt ctgaagctag cattacagca gcgcaagata gcaatctaga acctatgaaa aagctttttg atgatatctc caaccccata aaaacggctg ttttgaagga atgtataaat agcttaaaaa atattgatca caagaatatt aatgattgta ttattgtagc agcagataaa ggtttaacct ataagggaat ctatacagat aatagtccag atagtattat actcaaaacg gataatatct acatcatctg tagcaaaaat gatctcgcac cagaacaact aaaaacatta aagcttggtg atgaatgcac cattacagtt ccaatgaaac aaaatattga aaacactctc attccaaaag aaaaactacc ccctttaaca aagaatgcaa taattgaaaa tattaaaaaa gatactctta tccaagcaaa actacgacaa gttacacatt tatcaaaact tgtttacggc agctcaaaaa tattaaataa ccagctagag ttgattaata caaatcaaaa gtcaggcgaa cagctttccg cacaaatcac gtcttctcct caatctattt ctaagcttgc aggtataaaa atattcggta tgaaaaactc gatgcgtaaa aaagctgaag ataatattat aaagctcgct aactcaatta aaagttatgg gtctgctgta aaaaatgttg aaacaacaat gattcaacag cacaaatcag aacaaaaacg cctttcaaaa acagtaaaac tacccagcac aaaactgcaa aacattctta acttaccaga agaaaagcag aaagaagttt tattagagga aaaatcgctt gcttcttcga ttggcaaaga gctcactgat tttataagtt cactcaatgc tcgtctctca ccaagcgagc gtaaaatgat cctggaaagt aatcatcaac aatttgctaa aaatgttggc atatcagaaa gccaagcaga aacaatcatc aaaactgctc aaaaagctaa agaagtatac caaaaaatgc aaaaaccagc agttgatctt acaaaacatc ttattgtatc aggctaa
Details for BaclA.17330.g.B1.GE35681
| HARVESTED ON: | 7/10/2012 |
| SEQUENCED ON: | 2/22/2016 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHME KTLSNAAIAT ESISNQALPS HYNYPNSLTL KNKYGITNHK DFTNKCAHDS AKAAINLRQE EPPKKFDSSY LKYLHKQLFK ETFEWAGHTR DLPFTFADGT VGVMPEMIRS NWRTEQPIIF ATGNKVQDGL KNIDKMLIEK NNLQGLSHKE FIENIAEIFA CLNYTHPFRE GNGRTQRIFC EKLAQAAGYY LDFSVVTKER MSEASITAAQ DSNLEPMKKL FDDISNPIKT AVLKECINSL KNIDHKNIND CIIVAADKGL TYKGIYTDNS PDSIILKTDN IYIICSKNDL APEQLKTLKL GDECTITVPM KQNIENTLIP KEKLXPLTKN AIIENIKKDT LIQAKLRQVT HLSKLVYGSS KILNNQLELI XXNQKSGEQL SAQITSSPQS ISKLAGIKIF GMKNSMRKKA EDNIIKLANS IKSYGSAVKN VETTMIQQHK SEQKRLSKTV KLPSTKLQNI LNLPEEKQKE VLLEEKSLAS SIGKELTDFI SSLNARLSPS ERKMILESNH QQFAKNVGIS ESQAETIIKT AQKAKEVYQK MQKPAVDL
Validated NT Sequence
atggctcacc accaccacca ccatatggaa aaaacactct caaatgcagc aatagcaaca gaatcaatat ccaaccaagc tttacctagt cattataact atcctaatag tctcacactg aaaaataaat atggaataac aaaccataaa gactttacta ataaatgtgc gcatgattca gcgaaagcag ctattaattt gcgtcaagaa gaaccgccta aaaaatttga ttcttcctat ttaaaatatc ttcacaaaca attatttaag gaaacgtttg aatgggctgg acatacccgt gatcttccat ttacatttgc agatggtaca gttggcgtta tgccagaaat gataagatca aattggagaa cagaacaacc aattattttt gcaaccggca ataaagtgca agatggttta aaaaatatag ataagatgct tatcgaaaag aataatttac aaggcttatc gcacaaagaa tttattgaga atatagcaga aatatttgct tgccttaact atacgcatcc ttttagagaa ggcaatggac gtacacaacg gatattttgc gaaaagcttg ctcaagctgc aggttattat cttgactttt cagttgtgac aaaagaacgt atgtctgaag ctagcattac agcagcgcaa gatagcaatc tagaacctat gaaaaagctt tttgatgata tctccaaccc cataaaaacg gctgttttga aggaatgtat aaatagctta aaaaatattg atcacaagaa tattaatgat tgtattattg tagcagcaga taaaggttta acctataagg gaatctatac agataatagt ccagatagta ttatactcaa aacggataat atctacatca tctgtagcaa aaatgatctc gcaccagaac aactaaaaac attaaagctt ggtgatgaat gcaccattac agttccaatg aaacaaaata ttgaaaacac tctcattcca aaagaaaaac tacnnccttt aacaaagaat gcaataattg aaaatattaa aaaagatact cttatccaag caaaactacg acaagttaca catttatcaa aacttgttta cggcagctca aaaatattaa ataaccagct agagttgatt nnnnnnaatc aaaagtcagg cgaacagctt tccgcacaaa tcacgtcttc tcctcaatct atttctaagc ttgcaggtat aaaaatattc ggtatgaaaa actcgatgcg taaaaaagct gaagataata ttataaagct cgctaactca attaaaagtt atgggtctgc tgtaaaaaat gttgaaacaa caatgattca acagcacaaa tcagaacaaa aacgcctttc aaaaacagta aaactaccca gcacaaaact gcaaaacatt cttaacttac cagaagaaaa gcagaaagaa gttttattag aggaaaaatc gcttgcttct tcgattggca aagagctcac tgattttata agttcactca atgctcgtct ctcaccaagc gagcgtaaaa tgatcctgga aagtaatcat caacaatttg ctaaaaatgt tggcatatca gaaagccaag cagaaacaat catcaaaact gctcaaaaag ctaaagaagt ataccaaaaa atgcaaaaac cagcagttga tcttaca
Expected Protein Sequence
MAHHHHHHME KTLSNAAIAT ESISNQALPS HYNYPNSLTL KNKYGITNHK DFTNKCAHDS AKAAINLRQE EPPKKFDSSY LKYLHKQLFK ETFEWAGHTR DLPFTFADGT VGVMPEMIRS NWRTEQPIIF ATGNKVQDGL KNIDKMLIEK NNLQGLSHKE FIENIAEIFA CLNYTHPFRE GNGRTQRIFC EKLAQAAGYY LDFSVVTKER MSEASITAAQ DSNLEPMKKL FDDISNPIKT AVLKECINSL KNIDHKNIND CIIVAADKGL TYKGIYTDNS PDSIILKTDN IYIICSKNDL APEQLKTLKL GDECTITVPM KQNIENTLIP KEKLPPLTKN AIIENIKKDT LIQAKLRQVT HLSKLVYGSS KILNNQLELI NTNQKSGEQL SAQITSSPQS ISKLAGIKIF GMKNSMRKKA EDNIIKLANS IKSYGSAVKN VETTMIQQHK SEQKRLSKTV KLPSTKLQNI LNLPEEKQKE VLLEEKSLAS SIGKELTDFI SSLNARLSPS ERKMILESNH QQFAKNVGIS ESQAETIIKT AQKAKEVYQK MQKPAVDLTK HLIVSG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaacactctc aaatgcagca atagcaacag aatcaatatc caaccaagct ttacctagtc attataacta tcctaatagt ctcacactga aaaataaata tggaataaca aaccataaag actttactaa taaatgtgcg catgattcag cgaaagcagc tattaatttg cgtcaagaag aaccgcctaa aaaatttgat tcttcctatt taaaatatct tcacaaacaa ttatttaagg aaacgtttga atgggctgga catacccgtg atcttccatt tacatttgca gatggtacag ttggcgttat gccagaaatg ataagatcaa attggagaac agaacaacca attatttttg caaccggcaa taaagtgcaa gatggtttaa aaaatataga taagatgctt atcgaaaaga ataatttaca aggcttatcg cacaaagaat ttattgagaa tatagcagaa atatttgctt gccttaacta tacgcatcct tttagagaag gcaatggacg tacacaacgg atattttgcg aaaagcttgc tcaagctgca ggttattatc ttgacttttc agttgtgaca aaagaacgta tgtctgaagc tagcattaca gcagcgcaag atagcaatct agaacctatg aaaaagcttt ttgatgatat ctccaacccc ataaaaacgg ctgttttgaa ggaatgtata aatagcttaa aaaatattga tcacaagaat attaatgatt gtattattgt agcagcagat aaaggtttaa cctataaggg aatctataca gataatagtc cagatagtat tatactcaaa acggataata tctacatcat ctgtagcaaa aatgatctcg caccagaaca actaaaaaca ttaaagcttg gtgatgaatg caccattaca gttccaatga aacaaaatat tgaaaacact ctcattccaa aagaaaaact acccccttta acaaagaatg caataattga aaatattaaa aaagatactc ttatccaagc aaaactacga caagttacac atttatcaaa acttgtttac ggcagctcaa aaatattaaa taaccagcta gagttgatta atacaaatca aaagtcaggc gaacagcttt ccgcacaaat cacgtcttct cctcaatcta tttctaagct tgcaggtata aaaatattcg gtatgaaaaa ctcgatgcgt aaaaaagctg aagataatat tataaagctc gctaactcaa ttaaaagtta tgggtctgct gtaaaaaatg ttgaaacaac aatgattcaa cagcacaaat cagaacaaaa acgcctttca aaaacagtaa aactacccag cacaaaactg caaaacattc ttaacttacc agaagaaaag cagaaagaag ttttattaga ggaaaaatcg cttgcttctt cgattggcaa agagctcact gattttataa gttcactcaa tgctcgtctc tcaccaagcg agcgtaaaat gatcctggaa agtaatcatc aacaatttgc taaaaatgtt ggcatatcag aaagccaagc agaaacaatc atcaaaactg ctcaaaaagc taaagaagta taccaaaaaa tgcaaaaacc agcagttgat cttacaaaac atcttattgt atcaggctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaclA.17330.g.B2.GE37021
| HARVESTED ON: | 6/12/2013 |
| SEQUENCED ON: | 6/11/2013 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMP SHYNYPNSLT LKNKYGITNH KDFTNKCAHD SAKAAINLRQ EEPPKKFDSS YLKYLHKQLF KETFEWAGHT RDLPFTFADG TVGVMPEMIR SNWRTEQPII FATGNKVQDG LKNIDKMLIE KNNLQGLSHK EFIENIAEIF ACLNYTHPFR EGNGRTQRIF CEKLAQAAGY YLDFSVVTKE RMSEASITAA QDSNLEPMKK LFDDISNPIK TAVLKECINS L
Validated NT Sequence
atcttactca cttataagct atttatacat tccttcaaaa cagccgtttt tatggggttg gagatatcat caaaaagctt tttcataggt tctagattgc tatcttgcgc tgctgtaatg ctagcttcag acatacgttc ttttgtcaca actgaaaagt caagataata acctgcagct tgagcaagct tttcgcaaaa tatccgttgt gtacgtccat tgccttctct aaaaggatgc gtatagttaa ggcaagcaaa tatttctgct atattctcaa taaattcttt gtgcgataag ccttgtaaat tattcttttc gataagcatc ttatctatat tttttaaacc atcttgcact ttattgccgg ttgcaaaaat aattggttgt tctgttctcc aatttgatct tatcatttct ggcataacgc caactgtacc atctgcaaat gtaaatggaa gatcacgggt atgtccagcc cattcaaacg tttccttaaa taattgtttg tgaagatatt ttaaatagga agaatcaaat tttttaggcg gttcttcttg acgcaaatta atagctgctt tcgctgaatc atgcgcacat ttattagtaa agtctttatg gtttgttatt ccatatttat ttttcagtgt gagactatta ggatagttat aatgactagg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHPS HYNYPNSLTL KNKYGITNHK DFTNKCAHDS AKAAINLRQE EPPKKFDSSY LKYLHKQLFK ETFEWAGHTR DLPFTFADGT VGVMPEMIRS NWRTEQPIIF ATGNKVQDGL KNIDKMLIEK NNLQGLSHKE FIENIAEIFA CLNYTHPFRE GNGRTQRIFC EKLAQAAGYY LDFSVVTKER MSEASITAAQ DSNLEPMKKL FDDISNPIKT AVLKECINSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctagtc attataacta tcctaatagt ctcacactga aaaataaata tggaataaca aaccataaag actttactaa taaatgtgcg catgattcag cgaaagcagc tattaatttg cgtcaagaag aaccgcctaa aaaatttgat tcttcctatt taaaatatct tcacaaacaa ttatttaagg aaacgtttga atgggctgga catacccgtg atcttccatt tacatttgca gatggtacag ttggcgttat gccagaaatg ataagatcaa attggagaac agaacaacca attatttttg caaccggcaa taaagtgcaa gatggtttaa aaaatataga taagatgctt atcgaaaaga ataatttaca aggcttatcg cacaaagaat ttattgagaa tatagcagaa atatttgctt gccttaacta tacgcatcct tttagagaag gcaatggacg tacacaacgg atattttgcg aaaagcttgc tcaagctgca ggttattatc ttgacttttc agttgtgaca aaagaacgta tgtctgaagc tagcattaca gcagcgcaag atagcaatct agaacctatg aaaaagcttt ttgatgatat ctccaacccc ataaaaacgg ctgttttgaa ggaatgtata aatagcttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaclA.17330.g.B1.PW36267
| PURIFICATION DATe: | 9/26/2012 |
| CONCENTRATION: | 26.4mg/ml |
| OBSERVED MW: | 65kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME KTLSNAAIAT ESISNQALPS HYNYPNSLTL KNKYGITNHK DFTNKCAHDS AKAAINLRQE EPPKKFDSSY LKYLHKQLFK ETFEWAGHTR DLPFTFADGT VGVMPEMIRS NWRTEQPIIF ATGNKVQDGL KNIDKMLIEK NNLQGLSHKE FIENIAEIFA CLNYTHPFRE GNGRTQRIFC EKLAQAAGYY LDFSVVTKER MSEASITAAQ DSNLEPMKKL FDDISNPIKT AVLKECINSL KNIDHKNIND CIIVAADKGL TYKGIYTDNS PDSIILKTDN IYIICSKNDL APEQLKTLKL GDECTITVPM KQNIENTLIP KEKLXPLTKN AIIENIKKDT LIQAKLRQVT HLSKLVYGSS KILNNQLELI XXNQKSGEQL SAQITSSPQS ISKLAGIKIF GMKNSMRKKA EDNIIKLANS IKSYGSAVKN VETTMIQQHK SEQKRLSKTV KLPSTKLQNI LNLPEEKQKE VLLEEKSLAS SIGKELTDFI SSLNARLSPS ERKMILESNH QQFAKNVGIS ESQAETIIKT AQKAKEVYQK MQKPAVDL
Validated NT Sequence
atggctcacc accaccacca ccatatggaa aaaacactct caaatgcagc aatagcaaca gaatcaatat ccaaccaagc tttacctagt cattataact atcctaatag tctcacactg aaaaataaat atggaataac aaaccataaa gactttacta ataaatgtgc gcatgattca gcgaaagcag ctattaattt gcgtcaagaa gaaccgccta aaaaatttga ttcttcctat ttaaaatatc ttcacaaaca attatttaag gaaacgtttg aatgggctgg acatacccgt gatcttccat ttacatttgc agatggtaca gttggcgtta tgccagaaat gataagatca aattggagaa cagaacaacc aattattttt gcaaccggca ataaagtgca agatggttta aaaaatatag ataagatgct tatcgaaaag aataatttac aaggcttatc gcacaaagaa tttattgaga atatagcaga aatatttgct tgccttaact atacgcatcc ttttagagaa ggcaatggac gtacacaacg gatattttgc gaaaagcttg ctcaagctgc aggttattat cttgactttt cagttgtgac aaaagaacgt atgtctgaag ctagcattac agcagcgcaa gatagcaatc tagaacctat gaaaaagctt tttgatgata tctccaaccc cataaaaacg gctgttttga aggaatgtat aaatagctta aaaaatattg atcacaagaa tattaatgat tgtattattg tagcagcaga taaaggttta acctataagg gaatctatac agataatagt ccagatagta ttatactcaa aacggataat atctacatca tctgtagcaa aaatgatctc gcaccagaac aactaaaaac attaaagctt ggtgatgaat gcaccattac agttccaatg aaacaaaata ttgaaaacac tctcattcca aaagaaaaac tacnnccttt aacaaagaat gcaataattg aaaatattaa aaaagatact cttatccaag caaaactacg acaagttaca catttatcaa aacttgttta cggcagctca aaaatattaa ataaccagct agagttgatt nnnnnnaatc aaaagtcagg cgaacagctt tccgcacaaa tcacgtcttc tcctcaatct atttctaagc ttgcaggtat aaaaatattc ggtatgaaaa actcgatgcg taaaaaagct gaagataata ttataaagct cgctaactca attaaaagtt atgggtctgc tgtaaaaaat gttgaaacaa caatgattca acagcacaaa tcagaacaaa aacgcctttc aaaaacagta aaactaccca gcacaaaact gcaaaacatt cttaacttac cagaagaaaa gcagaaagaa gttttattag aggaaaaatc gcttgcttct tcgattggca aagagctcac tgattttata agttcactca atgctcgtct ctcaccaagc gagcgtaaaa tgatcctgga aagtaatcat caacaatttg ctaaaaatgt tggcatatca gaaagccaag cagaaacaat catcaaaact gctcaaaaag ctaaagaagt ataccaaaaa atgcaaaaac cagcagttga tcttaca
Expressed Protein Sequence
MAHHHHHHME KTLSNAAIAT ESISNQALPS HYNYPNSLTL KNKYGITNHK DFTNKCAHDS AKAAINLRQE EPPKKFDSSY LKYLHKQLFK ETFEWAGHTR DLPFTFADGT VGVMPEMIRS NWRTEQPIIF ATGNKVQDGL KNIDKMLIEK NNLQGLSHKE FIENIAEIFA CLNYTHPFRE GNGRTQRIFC EKLAQAAGYY LDFSVVTKER MSEASITAAQ DSNLEPMKKL FDDISNPIKT AVLKECINSL KNIDHKNIND CIIVAADKGL TYKGIYTDNS PDSIILKTDN IYIICSKNDL APEQLKTLKL GDECTITVPM KQNIENTLIP KEKLPPLTKN AIIENIKKDT LIQAKLRQVT HLSKLVYGSS KILNNQLELI NTNQKSGEQL SAQITSSPQS ISKLAGIKIF GMKNSMRKKA EDNIIKLANS IKSYGSAVKN VETTMIQQHK SEQKRLSKTV KLPSTKLQNI LNLPEEKQKE VLLEEKSLAS SIGKELTDFI SSLNARLSPS ERKMILESNH QQFAKNVGIS ESQAETIIKT AQKAKEVYQK MQKPAVDLTK HLIVSG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA AAACACTCTC AAATGCAGCA ATAGCAACAG AATCAATATC CAACCAAGCT TTACCTAGTC ATTATAACTA TCCTAATAGT CTCACACTGA AAAATAAATA TGGAATAACA AACCATAAAG ACTTTACTAA TAAATGTGCG CATGATTCAG CGAAAGCAGC TATTAATTTG CGTCAAGAAG AACCGCCTAA AAAATTTGAT TCTTCCTATT TAAAATATCT TCACAAACAA TTATTTAAGG AAACGTTTGA ATGGGCTGGA CATACCCGTG ATCTTCCATT TACATTTGCA GATGGTACAG TTGGCGTTAT GCCAGAAATG ATAAGATCAA ATTGGAGAAC AGAACAACCA ATTATTTTTG CAACCGGCAA TAAAGTGCAA GATGGTTTAA AAAATATAGA TAAGATGCTT ATCGAAAAGA ATAATTTACA AGGCTTATCG CACAAAGAAT TTATTGAGAA TATAGCAGAA ATATTTGCTT GCCTTAACTA TACGCATCCT TTTAGAGAAG GCAATGGACG TACACAACGG ATATTTTGCG AAAAGCTTGC TCAAGCTGCA GGTTATTATC TTGACTTTTC AGTTGTGACA AAAGAACGTA TGTCTGAAGC TAGCATTACA GCAGCGCAAG ATAGCAATCT AGAACCTATG AAAAAGCTTT TTGATGATAT CTCCAACCCC ATAAAAACGG CTGTTTTGAA GGAATGTATA AATAGCTTAA AAAATATTGA TCACAAGAAT ATTAATGATT GTATTATTGT AGCAGCAGAT AAAGGTTTAA CCTATAAGGG AATCTATACA GATAATAGTC CAGATAGTAT TATACTCAAA ACGGATAATA TCTACATCAT CTGTAGCAAA AATGATCTCG CACCAGAACA ACTAAAAACA TTAAAGCTTG GTGATGAATG CACCATTACA GTTCCAATGA AACAAAATAT TGAAAACACT CTCATTCCAA AAGAAAAACT ACCCCCTTTA ACAAAGAATG CAATAATTGA AAATATTAAA AAAGATACTC TTATCCAAGC AAAACTACGA CAAGTTACAC ATTTATCAAA ACTTGTTTAC GGCAGCTCAA AAATATTAAA TAACCAGCTA GAGTTGATTA ATACAAATCA AAAGTCAGGC GAACAGCTTT CCGCACAAAT CACGTCTTCT CCTCAATCTA TTTCTAAGCT TGCAGGTATA AAAATATTCG GTATGAAAAA CTCGATGCGT AAAAAAGCTG AAGATAATAT TATAAAGCTC GCTAACTCAA TTAAAAGTTA TGGGTCTGCT GTAAAAAATG TTGAAACAAC AATGATTCAA CAGCACAAAT CAGAACAAAA ACGCCTTTCA AAAACAGTAA AACTACCCAG CACAAAACTG CAAAACATTC TTAACTTACC AGAAGAAAAG CAGAAAGAAG TTTTATTAGA GGAAAAATCG CTTGCTTCTT CGATTGGCAA AGAGCTCACT GATTTTATAA GTTCACTCAA TGCTCGTCTC TCACCAAGCG AGCGTAAAAT GATCCTGGAA AGTAATCATC AACAATTTGC TAAAAATGTT GGCATATCAG AAAGCCAAGC AGAAACAATC ATCAAAACTG CTCAAAAAGC TAAAGAAGTA TACCAAAAAA TGCAAAAACC AGCAGTTGAT CTTACAAAAC ATCTTATTGT ATCAGGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT