BaclA.17330.e

Bep8 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaclA.17330.e
ORGANISM: Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.e.B3.GE37018 FIC and truncated OB-Fold 10 297
BaclA.17330.e.B4.GE37019 BID 308 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|696125.3.peg.596
RefSeq: YP_004158893.1
UniProt: E6YHH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTSSDISLA RNYAYPNSKT LKNKYGIMDF KKLEKKCTHD AKKVIVNLHQ EPLPEKFDSS YLKYLHKCLF EDAFEWAGHT RDLPFTFKDG TTAQMLMMKM PNSNIFFVAS NKIQESLQEF DQMLVAKNNL QGLSREEFID EAAKLFSSLN YIHPFRGGNE PTQQMFFEKL AEAAGHRIDF SIVTQERMIR ACTDAITLKG DEAHEAMKHL FEDISNPEKV RVLKELLNHI PRLECKRLDN EYVVMPREGM TYTGIYKGGS LDSIIVETVD SCVICHKDYL TPEQIKALKP GNRVTFTVPI NKDLDQILIP AEKLAPLREE EIIKKIKNTD CIKESRKGID HLLKLVYGDP TILDKHMNLI NKDPEMGQQL AEQIRNSPKS ISKLAGFKIW RIRSPKRRVA ERNISSLSRA ITQHVQIVKS VKDKIFIEYK RKQKRLEQVV KIPSKEVRES LNLPKDIQKK ALESSPSLHR DLCDFLCKVN SRLSMSELEH IRDTNHKILA ESIGISQSKA KIITQTFNKG VKLYKQFETI KADRSKIAAM TI
NT Sequence
atgacaacat cttccgatat atccttagcc cgtaattatg cctatcctaa cagtaaaaca ttaaagaata aatatggaat aatggatttt aaaaaacttg aaaagaaatg tacacatgat gcaaaaaaag taatcgtcaa tttgcatcaa gaaccgctac cagaaaagtt tgattcttct tatttaaaat atctccataa atgcttattt gaagacgcat ttgaatgggc cggacatacc cgcgatctcc cctttacatt taaagacggc acaactgccc aaatgttaat gatgaaaatg ccgaattcaa atattttttt tgtagctagt aacaaaattc aagaaagctt acaggaattt gaccaaatgc ttgttgcaaa gaataatttg caaggtttat cgcgcgaaga atttattgat gaggcagcaa aacttttttc ttctctcaac tatatacatc ctttcagagg tggtaatgaa cctacacaac aaatgttttt tgaaaagctt gcggaagctg caggtcatag aattgatttt tcaattgtga cacaagaacg catgatacgt gcttgtactg atgcgataac tctaaaaggt gatgaagctc atgaagcgat gaaacattta tttgaagata tttccaatcc agaaaaagta cgtgttttaa aggaattact caatcacata cctaggcttg agtgcaaacg tttggataat gaatatgttg taatgccacg tgaaggtatg acctatacag gtatttacaa aggaggtagt ttagattcta ttatagttga aacagtggat tcttgcgtca tatgtcataa agattatctt acaccagaac aaatcaaggc attaaaaccc ggtaatcggg ttacctttac agttccaata aataaagatt tagatcaaat tcttatccca gcagaaaagt tagctccttt gagggaagag gaaataatca aaaagattaa aaatactgat tgtattaaag aaagccgaaa aggaattgac catttattaa agcttgttta tggagatcca acaatattag ataagcatat gaatcttatt aataaagatc ctgaaatggg tcaacagctt gccgaacaga ttagaaattc tcctaaatct atttctaaac tcgcaggctt caaaatatgg cgtataagga gtccaaaacg cagggtagct gaaagaaata tttcaagcct cagtcgagca attacacaac atgtgcaaat tgtaaaatct gttaaagata aaatttttat agagtataaa agaaaacaaa aacgacttga gcaagtagta aaaataccta gtaaagaagt gcgagagagt cttaatttgc caaaggatat acaaaaaaaa gctttagagt cttctccttc acttcacaga gatctttgtg attttctgtg taaagtcaat tctcgtcttt caatgagtga gctagaacat attcgtgata ctaatcataa aatactggct gaaagtattg gcatatcaca aagtaaagcg aagataatta cacaaacttt taataaagga gtaaaattat acaaacaatt tgaaacaatc aaagctgacc gttcgaaaat agcggctatg accatctga
Details for BaclA.17330.e.B3.GE37018
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RNYAYPNSKT LKNKYGIMDF KKLEKKCTHD AKKVIVNLHQ EPLPEKFDSS YLKYLHKCLF EDAFEWAGHT RDLPFTFKDG TTAQMLMMKM PNSNIFFVAS NKIQESLQEF DQMLVAKNNL QGLSREEFID EAAKLFSSLN YIHPFRGGNE PTQQMFFEKL AEAAGHRIDF SIVTQERMIR ACTDAITLKG DEAHEAMKHL FEDISNPEKV RVLKELLNHI PRLECKRLDN EYVVMPREXM TYTGIYKGXS LDSIIVETVD SCVICHKXYL TPEQIKAXKP GNRVTFT
Validated NT Sequence
ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatggcccg taattatgcc tatcctaaca gtaaaacatt aaagaataaa tatggaataa tggattttaa aaaacttgaa aagaaatgta cacatgatgc aaaaaaagta atcgtcaatt tgcatcaaga accgctacca gaaaagtttg attcttctta tttaaaatat ctccataaat gcttatttga agacgcattt gaatgggccg gacatacccg cgatctcccc tttacattta aagacggcac aactgcccaa atgttaatga tgaaaatgcc gaattcaaat attttttttg tagctagtaa caaaattcaa gaaagcttac aggaatttga ccaaatgctt gttgcaaaga ataatttgca aggtttatcg cgcgaagaat ttattgatga ggcagcaaaa cttttttctt ctctcaacta tatacatcct ttcagaggtg gtaatgaacc tacacaacaa atgttttttg aaaagcttgc ggaagctgca ggtcatagaa ttgatttttc aattgtgaca caagaacgca tgatacgtgc ttgtactgat gcgataactc taaaaggtga tgaagctcat gaagcgatga aacatttatt tgaagatatt tccaatccag aaaaagtacg tgttttaaag gaattactca atcacatacc taggcttgag tgcaaacgtt tggataatga atatgttgta atgccacgtg aangtatgac ctatacaggt atttacaaag gangtagttt agattctatt atagttgaaa cagtggattc ttgcgtcata tgtcataaag antatcttac accagaacaa atcaaggcan taaaacccgg taatcgggtt acctttacat aagtgagtaa nataggatcc ggctgctaac aaagcccgaa angaanctga nttggctgct gcnnngntga ncaatantag nataancccn tngnggcntc taanngnnnn nnnnnagng
Expected Protein Sequence
MAHHHHHHAR NYAYPNSKTL KNKYGIMDFK KLEKKCTHDA KKVIVNLHQE PLPEKFDSSY LKYLHKCLFE DAFEWAGHTR DLPFTFKDGT TAQMLMMKMP NSNIFFVASN KIQESLQEFD QMLVAKNNLQ GLSREEFIDE AAKLFSSLNY IHPFRGGNEP TQQMFFEKLA EAAGHRIDFS IVTQERMIRA CTDAITLKGD EAHEAMKHLF EDISNPEKVR VLKELLNHIP RLECKRLDNE YVVMPREGMT YTGIYKGGSL DSIIVETVDS CVICHKDYLT PEQIKALKPG NRVTFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcccgta attatgccta tcctaacagt aaaacattaa agaataaata tggaataatg gattttaaaa aacttgaaaa gaaatgtaca catgatgcaa aaaaagtaat cgtcaatttg catcaagaac cgctaccaga aaagtttgat tcttcttatt taaaatatct ccataaatgc ttatttgaag acgcatttga atgggccgga catacccgcg atctcccctt tacatttaaa gacggcacaa ctgcccaaat gttaatgatg aaaatgccga attcaaatat tttttttgta gctagtaaca aaattcaaga aagcttacag gaatttgacc aaatgcttgt tgcaaagaat aatttgcaag gtttatcgcg cgaagaattt attgatgagg cagcaaaact tttttcttct ctcaactata tacatccttt cagaggtggt aatgaaccta cacaacaaat gttttttgaa aagcttgcgg aagctgcagg tcatagaatt gatttttcaa ttgtgacaca agaacgcatg atacgtgctt gtactgatgc gataactcta aaaggtgatg aagctcatga agcgatgaaa catttatttg aagatatttc caatccagaa aaagtacgtg ttttaaagga attactcaat cacataccta ggcttgagtg caaacgtttg gataatgaat atgttgtaat gccacgtgaa ggtatgacct atacaggtat ttacaaagga ggtagtttag attctattat agttgaaaca gtggattctt gcgtcatatg tcataaagat tatcttacac cagaacaaat caaggcatta aaacccggta atcgggttac ctttacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaclA.17330.e.B4.GE37019
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPAEKLAPLR EEEIIKKIKN TDCIKESRKG IDHLLKLVYG DPTILDKHMN LINKDPEMGQ QLAEQIRNSP KSISKLAGFK IWRIRSPKRR VAERNISSLS RAITQHVQIV KSVKDKIFIE YKRKQKRLEQ VVKIPSK
Validated NT Sequence
atcttactca cttatttact aggtattttt actacttgct caagtcgttt ttgttttctt ttatactcta taaaaatttt atctttaaca gattttacaa tttgcacatg ttgtgtaatt gctcgactga ggcttgaaat atttctttca gctaccctgc gttttggact ccttatacgc catattttga agcctgcgag tttagaaata gatttaggag aatttctaat ctgttcggca agctgttgac ccatttcagg atctttatta ataagattca tatgcttatc taatattgtt ggatctccat aaacaagctt taataaatgg tcaattcctt ttcggctttc tttaatacaa tcagtatttt taatcttttt gattatttcc tcttccctca aaggagctaa cttttctgct gggataagca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHLI PAEKLAPLRE EEIIKKIKNT DCIKESRKGI DHLLKLVYGD PTILDKHMNL INKDPEMGQQ LAEQIRNSPK SISKLAGFKI WRIRSPKRRV AERNISSLSR AITQHVQIVK SVKDKIFIEY KRKQKRLEQV VKIPSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttatcc cagcagaaaa gttagctcct ttgagggaag aggaaataat caaaaagatt aaaaatactg attgtattaa agaaagccga aaaggaattg accatttatt aaagcttgtt tatggagatc caacaatatt agataagcat atgaatctta ttaataaaga tcctgaaatg ggtcaacagc ttgccgaaca gattagaaat tctcctaaat ctatttctaa actcgcaggc ttcaaaatat ggcgtataag gagtccaaaa cgcagggtag ctgaaagaaa tatttcaagc ctcagtcgag caattacaca acatgtgcaa attgtaaaat ctgttaaaga taaaattttt atagagtata aaagaaaaca aaaacgactt gagcaagtag taaaaatacc tagtaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at