SSGCID: Target Page

BaclA.17330.d

Bep10 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.d
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.d.B2.GE37014	FIC		23	262
BaclA.17330.d.B3.GE37015	FIC and truncated OB-Fold		23	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.983
RefSeq:	YP_004159283.1
UniProt:	E6YIM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIKNAADLK QAKQLLLEQS HNYTYNNGVL KNKLNIQNNE AFRKQCAHLV AKEFINAHQE PPPKNIDVSY LKKLHQRLFC NIFDWAGKTR NESIKMSDGT IASTSTSDGK KNQALVFASN EKLENCLNEL NSKLVDRNNK VSVDRNNKVS SENKTQEEKK RGLSEDITTI YALLNHARPF VQGNGVTQHL FMQKLSQAEG IKIDLAAVTP KRMEIANVEA ANDNLEPMKH LFEDASNPEK AHVLKETLNG IKSKNQDKTL AEALVTTPIP GQTYTGTFES RQLDAIVIKT ENSLIVCNRH VIPRHKFKNL KIGDTITFKA QEDLNKVFIP GREIPDLTED KVTEMAILSS SVQEQKKEVD HFAACVFGHR EKISSVMERV YKNPNFSQQL SIQILHNPKS IAKLAGFQKF GIKSPKRKEA EENIFKLSEA IKDLADSLRI SKEEVIKNHK EEQQRVGKTI PLPTEEVKLF LSMSSKERVN VLKTEGNPTL RRELASFLEQ VNSRLSKDEH KMISNNDYAS FAQSLDMPEK FAKQIMETVK QAKEMQNDIK LIKPPRQQKA MAIAS

NT Sequence

atgtcgataa aaaacgccgc tgatttgaaa caagcaaagc aattattatt ggaacaaagc cataattata cctataacaa cggtgtatta aaaaataaac ttaatataca aaacaatgag gcctttagaa agcaatgtgc gcatttagta gcaaaagaat ttattaatgc gcaccaagaa ccgccaccaa aaaatattga cgtttcttat ctaaaaaagc ttcatcagcg cttattttgc aacatatttg attgggctgg aaaaactcgt aatgagtcaa ttaaaatgtc agatggtaca attgctagta ctagcacatc agacggaaaa aaaaatcaag ctcttgtttt tgcatctaat gaaaaactcg aaaactgctt aaatgagctg aattcgaagc ttgttgatag aaataataaa gtctcagttg atagaaataa taaagtctca tcggaaaata aaacgcaaga agaaaaaaag agaggattat ccgaggatat aacgactatt tatgcattac tcaaccatgc gcgtcccttt gtacagggta atggagttac acaacacttg tttatgcaaa aactctctca agctgagggt attaagatag atcttgcagc tgtgacacca aaacgtatgg aaattgcaaa tgtcgaggca gcaaatgaca atttagagcc tatgaaacat ctgtttgaag atgcttctaa tccagaaaaa gctcatgttt taaaagaaac cttaaatgga attaagtcca aaaaccaaga taaaactctc gcagaagcac ttgttacaac gccaattcca gggcaaacct acacaggtac ctttgaaagt cgtcaactgg acgctattgt gatcaaaaca gaaaattctt tgattgtatg taatagacac gtcattccaa ggcacaaatt taaaaattta aaaataggtg atacaataac atttaaggct caagaagact taaacaaagt ttttatccca ggaagagaaa tacctgattt aacagaagat aaagttactg aaatggctat attgagctct tctgttcaag agcaaaaaaa agaagttgat cattttgcag catgtgtttt tggacacagg gaaaagatca gctctgtaat ggaaagagtt tataaaaacc caaatttttc acaacaactt tccatacaga ttctacataa tcctaaatct attgctaagc ttgcaggttt tcaaaaattt ggaataaaaa gtccaaaacg taaagaagct gaagaaaata tttttaaact cagtgaagca attaaagatt tagcggattc tctgagaatc tctaaggaag aagttattaa aaatcataaa gaagaacagc agcgcgtagg aaaaacaata ccgctgccta cagaagaagt aaagttgttt cttagtatgt catcaaaaga acgtgtaaat gttttaaaaa cagagggaaa tcctactctt cgcagagaat tggctagttt tcttgagcaa gtcaattctc gtctttcaaa agatgaacat aaaatgatta gtaataacga ttatgcatca tttgctcaaa gtcttgacat gccagagaaa tttgcaaagc aaattatgga aactgttaag caagccaaag aaatgcaaaa tgatattaaa ttaatcaaac ctcctcgcca acaaaaagcg atggctatag ccagctga

Details for BaclA.17330.d.B2.GE37014

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/19/2013
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY TYNNGVLKNK LNIQNNEAFR KQCAHLVAKE FINAHQEPPP KNIDVSYLKK LHQRLFCNIF DWAGKTRNES IKMSDGTIAS TSTSDGKKNQ ALVFASNEKL ENCLNELNSK LVDRNNKVSV DRNNKVSSEN KTQEEKKRGL SEDITTIYAL LNHARPFVQG NGVTQHLFMQ KLSQAEGIKI DLAAVTPKRM EIANVEAAND NLEPMKHLFE DASNPEKAHV LKETLNGIKS KNQDKTLAE

Validated NT Sequence

atggctcacc accaccacca ccatatgtat acctataaca acggtgtatt aaaaaataaa cttaatatac aaaacaatga ggcctttaga aagcaatgtg cgcatttagt agcaaaagaa tttattaatg cgcaccaaga accgccacca aaaaatattg acgtttctta tctaaaaaag cttcatcagc gcttattttg caacatattt gattgggctg gaaaaactcg taatgagtca attaaaatgt cagatggtac aattgctagt actagcacat cagacggaaa aaaaaatcaa gctcttgttt ttgcatctaa tgaaaaactc gaaaactgct taaatgagct gaattcgaag cttgttgata gaaataataa agtctcagtt gatagaaata ataaagtctc atcggaaaat aaaacgcaag aagaaaaaaa gagaggatta tccgaggata taacgactat ttatgcatta ctcaaccatg cgcgtccctt tgtacagggt aatggagtta cacaacactt gtttatgcaa aaactctctc aagctgaggg tattaagata gatcttgcag ctgtgacacc aaaacgtatg gaaattgcaa atgtcgaggc agcaaatgac aatttagagc ctatgaaaca tctgtttgaa gatgcttcta atccagaaaa agctcatgtt ttaaaagaaa ccttaaatgg aattaagtcc aaaaaccaag ataaaactct cgcagaataa gtgagtaaga t

Expected Protein Sequence

MAHHHHHHYT YNNGVLKNKL NIQNNEAFRK QCAHLVAKEF INAHQEPPPK NIDVSYLKKL HQRLFCNIFD WAGKTRNESI KMSDGTIAST STSDGKKNQA LVFASNEKLE NCLNELNSKL VDRNNKVSVD RNNKVSSENK TQEEKKRGLS EDITTIYALL NHARPFVQGN GVTQHLFMQK LSQAEGIKID LAAVTPKRME IANVEAANDN LEPMKHLFED ASNPEKAHVL KETLNGIKSK NQDKTLAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatacct ataacaacgg tgtattaaaa aataaactta atatacaaaa caatgaggcc tttagaaagc aatgtgcgca tttagtagca aaagaattta ttaatgcgca ccaagaaccg ccaccaaaaa atattgacgt ttcttatcta aaaaagcttc atcagcgctt attttgcaac atatttgatt gggctggaaa aactcgtaat gagtcaatta aaatgtcaga tggtacaatt gctagtacta gcacatcaga cggaaaaaaa aatcaagctc ttgtttttgc atctaatgaa aaactcgaaa actgcttaaa tgagctgaat tcgaagcttg ttgatagaaa taataaagtc tcagttgata gaaataataa agtctcatcg gaaaataaaa cgcaagaaga aaaaaagaga ggattatccg aggatataac gactatttat gcattactca accatgcgcg tccctttgta cagggtaatg gagttacaca acacttgttt atgcaaaaac tctctcaagc tgagggtatt aagatagatc ttgcagctgt gacaccaaaa cgtatggaaa ttgcaaatgt cgaggcagca aatgacaatt tagagcctat gaaacatctg tttgaagatg cttctaatcc agaaaaagct catgttttaa aagaaacctt aaatggaatt aagtccaaaa accaagataa aactctcgca gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BaclA.17330.d.B3.GE37015

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	34kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

atcttactca cttacttaaa tgttattgta tcacctattt ttaaattttt aaatttgtgc cttggaatga cgtgtctatt acatacaatc aaagaatttt ctgttttgat cacaatagcg tccagttgac gactttcaaa ggtacctgtg taggtttgcc ctggaattgg cgttgtaaca agtgcttctg cgagagtttt atcttggttt ttggacttaa ttccatttaa ggtttctttt aaaacatgag ctttttctgg attagaagca tcttcaaaca gatgtttcat aggctctaaa ttgtcatttg ctgcctcgac atttgcaatt tccatacgtt ttggtgtcac agctgcaaga tctatcttaa taccctcagc ttgagagagt ttttgcataa acaagtgttg tgtaactcca ttaccctgta caaagggacg cgcatggttg agtaatgcat aaatagtcgt tatatcctcg gataatcctc tctttttttc ttcttgcgtt ttattttccg atgagacttt attatttcta tcaactgaga ctttattatt tctatcaaca agcttcgaat tcagctcatt taagcagttt tcgagttttt cattagatgc aaaaacaaga gcttgatttt tttttccgtc tgatgtgcta gtactagcaa ttgtaccatc tgacatttta attgactcat tacgagtttt tccagcccaa tcaaatatgt tgcaaaataa gcgctgatga agctttttta gataagaaac gtcaatattt tttggtggcg gttcttggtg cgcattaata aattcttttg ctactaaatg cgcacattgc tttctaaagg cctcattgtt ttgtatatta agtttatttt ttaatacacc gttgttatag gtatacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatacct ataacaacgg tgtattaaaa aataaactta atatacaaaa caatgaggcc tttagaaagc aatgtgcgca tttagtagca aaagaattta ttaatgcgca ccaagaaccg ccaccaaaaa atattgacgt ttcttatcta aaaaagcttc atcagcgctt attttgcaac atatttgatt gggctggaaa aactcgtaat gagtcaatta aaatgtcaga tggtacaatt gctagtacta gcacatcaga cggaaaaaaa aatcaagctc ttgtttttgc atctaatgaa aaactcgaaa actgcttaaa tgagctgaat tcgaagcttg ttgatagaaa taataaagtc tcagttgata gaaataataa agtctcatcg gaaaataaaa cgcaagaaga aaaaaagaga ggattatccg aggatataac gactatttat gcattactca accatgcgcg tccctttgta cagggtaatg gagttacaca acacttgttt atgcaaaaac tctctcaagc tgagggtatt aagatagatc ttgcagctgt gacaccaaaa cgtatggaaa ttgcaaatgt cgaggcagca aatgacaatt tagagcctat gaaacatctg tttgaagatg cttctaatcc agaaaaagct catgttttaa aagaaacctt aaatggaatt aagtccaaaa accaagataa aactctcgca gaagcacttg ttacaacgcc aattccaggg caaacctaca caggtacctt tgaaagtcgt caactggacg ctattgtgat caaaacagaa aattctttga ttgtatgtaa tagacacgtc attccaaggc acaaatttaa aaatttaaaa ataggtgata caataacatt taagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat