SSGCID: Target Page

BaclA.17330.c

Bep3 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.c
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.c.B3.GE37012	FIC and truncated OB-Fold		98	392
BaclA.17330.c.B4.GE37013	BID		406	543

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.c.AE6.PW37662	fused antitoxin FIC		1	310

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.603
RefSeq:	YP_004158900.1
UniProt:	E6YHI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPIKLFNNSV SLTAEELQKR RRIVDSAIHT HTIENITLHS DTLLTLERYA EGDYSLDEFN IIMDTVERII LDDMQITITE EPQVEETIIS SNFNSPSPYN YIYPRSWVLK NKHGIKDNQV LGAVSGHHVV KAILNLHCEP LPERFDSSYL KYLHKRLFEN TFEWAGNTRD VTFTFADGTA AIMPTMKKLN SNNSFLTSER IPKELKNIDK ILAEHDNLKG LSRQDFVHKA TGIFTFLNHI HPFRDGNGRA QRMFFEKLAE AAGHQLDFSV ITAERMVVSS MVSITDTGNI GDIRAIGHML EDISNPENVY VLKEFMNSMN DIERKNAQKK IILTPNKDEI YTGIYENDSP NSILLKTDRD YIAKPLYMIF KKDYFSPEQL KTLKVGNQFS FTVPIKENVK DLENLLIPKE RLAFLTEEQI VERITNHHDV QSKREEINFY AKRVYRKLKK FNAKIEMISQ NTQFGLELVK QITNSPESIS KLAGKKILGI GNSKHEIAKQ NIYILVQKID EYVNTVKRVK SVILGEYLIE QQRLATVVEM PSKEIQNIFN LPKDMQKEAL KSSPSLHEEL NNFIKKIRCR LTLKEYKLLR DANYKELAES VGISENRAKQ IKEIFTQSKI LQNSLKQVRF GNAQTMNMVS

NT Sequence

atgccaataa aactatttaa taattctgta tctttgacag cagaagaatt acaaaaacgt cgccgaatcg ttgattcggc gattcacact cacactattg aaaatattac tttgcattct gatacacttc taactttaga aagatatgca gaaggagatt attcattaga tgaatttaat attattatgg atactgttga acgaattatt ctggacgata tgcagattac aattacagaa gaaccgcaag tggaagaaac cataatatca tctaacttta attccccatc accttacaat tatatctatc ctagaagttg ggtactgaaa aataaacacg gaataaaaga caatcaagtt cttggtgcag taagtggaca tcacgtggtg aaggcaatcc ttaacctaca ctgtgaacct ctaccagaac ggtttgattc ttcttatcta aaatatcttc ataaacgctt gtttgaaaat acatttgaat gggctggaaa tactcgcgat gttacattta catttgcaga tggaacagct gctattatgc caacaatgaa aaaattgaac tcaaacaatt cttttctaac aagtgagaga attcccaaag aactaaaaaa tatagacaaa atacttgcag agcacgataa ccttaaagga ctatcgcgtc aagattttgt tcataaagca acgggtatat ttacttttct taatcacata catccattca gagatggtaa tgggcgtgca caacgaatgt tttttgaaaa actcgctgaa gctgcaggtc atcaacttga tttttcagtt atcacagcag aacgtatggt ggtttccagt atggtttcta taacggatac tggtaatata ggtgatataa gagctatagg acatatgctt gaagatattt ctaatccaga aaacgtatat gttttaaagg aattcatgaa ctccatgaat gatatcgagc gtaaaaatgc gcagaaaaaa attattttaa cgccaaataa agatgaaatt tatacaggca tctatgaaaa tgatagccca aattctattc tactcaaaac agatcgtgat tatattgcaa aaccgcttta tatgatcttt aagaaagatt atttttcacc agaacaactt aagacattaa aagtcggtaa tcagttttcc tttacagttc caataaaaga aaatgtaaaa gatttagaaa accttctcat tccaaaagaa agattagctt ttttaactga agagcaaatt gttgaaagga ttacaaatca tcacgatgtc caatcaaaac gagaagagat caatttttat gcaaagcgtg tttatcgcaa gttaaaaaaa tttaacgcaa agatagagat gattagtcaa aatacacagt ttggtctaga gcttgttaag cagattacta attctcctga atctatatct aaattagcag gtaagaaaat attaggcata ggaaactcaa aacacgaaat agctaaacaa aatatttata tacttgttca aaaaattgat gaatatgtga atactgtaaa acgtgtgaaa agtgtaattc ttggagagta tctaattgaa caacaacgtc ttgcgacagt agtggaaatg cccagcaaag aaatacaaaa tatttttaac ttgccaaaag atatgcagaa agaagctttg aagtcctctc cttcgctgca cgaagaactc aataatttta taaaaaaaat ccgttgccgt cttacattaa aagagtataa attgctccgt gatgctaact ataaagaact cgctgaaagc gttggcatat cagaaaatag agcaaagcaa attaaagaaa tctttacgca aagcaaaata ttacaaaatt cattgaaaca agtcagattt ggtaacgcac aaacaatgaa tatggttagc taa

Details for BaclA.17330.c.B3.GE37012

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP YNYIYPRSWV LKNKHGIKDN QVLGAVSGHH VVKAILNLHC EPLPERFDSS YLKYLHKRLF ENTFEWAGNT RDVTFTFADG TAAIMPTMKK LNSNNSFLTS ERIPKELKNI DKILAEHDNL KGLSRQDFVH KATGIFTFLN HIHPFRDGNG RAQRMFFEKL AEAAGHQLDF SVITAERMVV SSMVSITDTG NIGDIRAIGH MLEDISNPEN VYVLKEFMNS MNDIERKNAQ KKIILTXNKD EIYTGIYEND SPNSILLKTD RDYIAKPLYM IFKKXIFHQN NLRH

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgcct tacaattata tctatcctag aagttgggta ctgaaaaata aacacggaat aaaagacaat caagttcttg gtgcagtaag tggacatcac gtggtgaagg caatccttaa cctacactgt gaacctctac cagaacggtt tgattcttct tatctaaaat atcttcataa acgcttgttt gaaaatacat ttgaatgggc tggaaatact cgcgatgtta catttacatt tgcagatgga acagctgcta ttatgccaac aatgaaaaaa ttgaactcaa acaattcttt tctaacaagt gagagaattc ccaaagaact aaaaaatata gacaaaatac ttgcagagca cgataacctt aaaggactat cgcgtcaaga ttttgttcat aaagcaacgg gtatatttac ttttcttaat cacatacatc cattcagaga tggtaatggg cgtgcacaac gaatgttttt tgaaaaactc gctgaagctg caggtcatca acttgatttt tcagttatca cagcagaacg tatggtggtt tccagtatgg tttctataac ggatactggt aatataggtg atataagagc tataggacat atgcttgaag atatttctaa tccagaaaac gtatatgttt taaaggaatt catgaactcc atgaatgata tcgagcgtaa aaatgcgcag aaaaaaatta ttttaacgcn naataaagat gaaatttata caggcatcta tgaaaatgat agcccaaatt ctattctact caaaacagat cgtgattata ttgcaaaacc gctttatatg atctttaaaa agantatttt tcaccagaac aacttaagac attaaaagtc ggtaatcagt tttcctttac ataagtgagt aaganaggan cnngctgcta acaaagcccn aaaggaagct ganttggctg ctgcnancnc tgancatanc tagcataacc ccttggggnn nnaannnnnn nnnnnng

Expected Protein Sequence

MAHHHHHHPY NYIYPRSWVL KNKHGIKDNQ VLGAVSGHHV VKAILNLHCE PLPERFDSSY LKYLHKRLFE NTFEWAGNTR DVTFTFADGT AAIMPTMKKL NSNNSFLTSE RIPKELKNID KILAEHDNLK GLSRQDFVHK ATGIFTFLNH IHPFRDGNGR AQRMFFEKLA EAAGHQLDFS VITAERMVVS SMVSITDTGN IGDIRAIGHM LEDISNPENV YVLKEFMNSM NDIERKNAQK KIILTPNKDE IYTGIYENDS PNSILLKTDR DYIAKPLYMI FKKDYFSPEQ LKTLKVGNQF SFT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccttaca attatatcta tcctagaagt tgggtactga aaaataaaca cggaataaaa gacaatcaag ttcttggtgc agtaagtgga catcacgtgg tgaaggcaat ccttaaccta cactgtgaac ctctaccaga acggtttgat tcttcttatc taaaatatct tcataaacgc ttgtttgaaa atacatttga atgggctgga aatactcgcg atgttacatt tacatttgca gatggaacag ctgctattat gccaacaatg aaaaaattga actcaaacaa ttcttttcta acaagtgaga gaattcccaa agaactaaaa aatatagaca aaatacttgc agagcacgat aaccttaaag gactatcgcg tcaagatttt gttcataaag caacgggtat atttactttt cttaatcaca tacatccatt cagagatggt aatgggcgtg cacaacgaat gttttttgaa aaactcgctg aagctgcagg tcatcaactt gatttttcag ttatcacagc agaacgtatg gtggtttcca gtatggtttc tataacggat actggtaata taggtgatat aagagctata ggacatatgc ttgaagatat ttctaatcca gaaaacgtat atgttttaaa ggaattcatg aactccatga atgatatcga gcgtaaaaat gcgcagaaaa aaattatttt aacgccaaat aaagatgaaa tttatacagg catctatgaa aatgatagcc caaattctat tctactcaaa acagatcgtg attatattgc aaaaccgctt tatatgatct ttaagaaaga ttatttttca ccagaacaac ttaagacatt aaaagtcggt aatcagtttt cctttacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BaclA.17330.c.B4.GE37013

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IPKERLAFLT EEQIVERITN HHDVQSKREE INFYAKRVYR KLKKFNAKIE MISQNTQFGL ELVKQITNSP ESISKLAGKK ILGIGNSKHE IAKQNIYILV QKIDEYVNTV KRVKSVILGE YLIEQQRLAT VVEMPSK

Validated NT Sequence

atcttactca cttatttgct gggcatttcc actactgtcg caagacgttg ttgttcaatt agatactctc caagaattac acttttcaca cgttttacag tattcacata ttcatcaatt ttttgaacaa gtatataaat attttgttta gctatttcgt gttttgagtt tcctatgcct aatattttct tacctgctaa tttagatata gattcaggag aattagtaat ctgcttaaca agctctagac caaactgtgt attttgacta atcatctcta tctttgcgtt aaattttttt aacttgcgat aaacacgctt tgcataaaaa ttgatctctt ctcgttttga ttggacatcg tgatgatttg taatcctttc aacaatttgc tcttcagtta aaaaagctaa tctttctttt ggaatgagca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHLI PKERLAFLTE EQIVERITNH HDVQSKREEI NFYAKRVYRK LKKFNAKIEM ISQNTQFGLE LVKQITNSPE SISKLAGKKI LGIGNSKHEI AKQNIYILVQ KIDEYVNTVK RVKSVILGEY LIEQQRLATV VEMPSK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcattc caaaagaaag attagctttt ttaactgaag agcaaattgt tgaaaggatt acaaatcatc acgatgtcca atcaaaacga gaagagatca atttttatgc aaagcgtgtt tatcgcaagt taaaaaaatt taacgcaaag atagagatga ttagtcaaaa tacacagttt ggtctagagc ttgttaagca gattactaat tctcctgaat ctatatctaa attagcaggt aagaaaatat taggcatagg aaactcaaaa cacgaaatag ctaaacaaaa tatttatata cttgttcaaa aaattgatga atatgtgaat actgtaaaac gtgtgaaaag tgtaattctt ggagagtatc taattgaaca acaacgtctt gcgacagtag tggaaatgcc cagcaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BaclA.17330.c.AE6.PW37662

PURIFICATION DATe:	3/3/2015
CONCENTRATION:	18mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GCCAATAAAA CTATTTAATA ATTCTGTATC TTTGACAGCA GAAGAATTAC AAAAACGTCG CCGAATCGTT GATTCGGCGA TTCACACTCA CACTATTGAA AATATTACTT TGCATTCTGA TACACTTCTA ACTTTAGAAA GATATGCAGA AGGAGATTAT TCATTAGATG AATTTAATAT TATTATGGAT ACTGTTGAAC GAATTATTCT GGACGATATG CAGATTACAA TTACAGAAGA ACCGCAAGTG GAAGAAACCA TAATATCATC TAACTTTAAT TCCCCATCAC CTTACAATTA TATCTATCCT AGAAGTTGGG TACTGAAAAA TAAACACGGA ATAAAAGACA ATCAAGTTCT TGGTGCAGTA AGTGGACATC ACGTGGTGAA GGCAATCCTT AACCTACACT GTGAACCTCT ACCAGAACGG TTTGATTCTT CTTATCTAAA ATATCTTCAT AAACGCTTGT TTGAAAATAC ATTTGAATGG GCTGGAAATA CTCGCGATGT TACATTTACA TTTGCAGATG GAACAGCTGC TATTATGCCA ACAATGAAAA AATTGAACTC AAACAATTCT TTTCTAACAA GTGAGAGAAT TCCCAAAGAA CTAAAAAATA TAGACAAAAT ACTTGCAGAG CACGATAACC TTAAAGGACT ATCGCGTCAA GATTTTGTTC ATAAAGCAAC GGGTATATTT ACTTTTCTTA ATCACATACA TCCATTCAGA GATGGTAATG GGCGTGCACA ACGAATGTTT TTTGAAAAAC TCGCTGAAGC TGCAGGTCAT CAACTTGATT TTTCAGTTAT CACAGCAGAA CGTATGGTGG TTTCCAGTAT GGTTTCTATA ACGGATACTG GTAATATAGG TGATATAAGA GCTATAGGAC ATATGCTTGA AGATATTTCT AATCCAGAAA ACGTATATGG GCACCACCAT CATCATCATT AACGGATCCG AATTCGAGCT CCGTCGACAA GCTTGCGGCC GCACTCGAGC ACCACCACCA CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGAT