BaclA.17330.b

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaclA.17330.b
ORGANISM: Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.b.B1.GE35676 full length 1 440
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaclA.17330.b.B1.PS01656 full length 1 440

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|696125.3.peg.244
RefSeq: YP_004158525.1
UniProt: E6YGE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARSDKIEEG LKIIDQKLSR KNNWRNLSRE EFVHNASVTF AFLHNLQPFV AGNGCAQRIF FEKIAEAAGH KLDFSVVTEA RMQFAGSAAL SFGDNEGNVA PLRHLFEDIS NPEKVSVLKE FIQYTSDIKG EDIRDKVIVM PNEGETYTGF YKSCSLDSIM LMVKDSYVLC KKDYLPPEKL KTLKLGDSIT FTASISKDCE NILIPEERLA SLTEEEIIEK IQKDFFVQKC RKEIKSLSKL IYGNSEMLNE HMDMINIGSS AGEQNVRYAV TDLPKPILKS SGTKMLGRAD PKYQQTEKNV FYLDQKINSY ISAVLHSRKR ILKEHSLEQE RCGQVVKMPC KTVQDILSMS GNISIEILNS DFLLCKHLQD FMCKVGSRLS QSERKAIGDN NYNVLAESIG ISVSKAKRVT QIIKRAQEVC QKEKVFKADR SRIMATGFSK
NT Sequence
ttggcaagga gtgataaaat tgaagaaggt ttgaaaataa tcgaccaaaa gctttccagg aagaataatt ggcggaattt atcgcgtgaa gaatttgttc ataacgcatc agtaacattt gcatttctcc ataatttaca gccttttgta gctgggaatg gatgcgcaca acgaatattt tttgaaaaaa tcgctgaggc tgcaggtcat aagcttgact tctcggttgt gacagaagca cgtatgcaat ttgccggtag tgctgcgtta tcattcggtg ataatgaagg caatgtagcc cctctgcgtc atctttttga ggatatttcc aatccagaaa aagtgagtgt tttgaaagaa ttcatacaat atacaagtga tattaaaggt gaagatatta gagataaagt tattgtaatg ccaaatgaag gcgaaaccta tacgggattc tataaaagtt gtagtctaga ttctattatg ctcatggtga aagatagtta tgttttatgc aaaaaagatt atcttccacc agaaaaatta aagacattaa aactcggtga tagtattacc tttacagctt caatatcaaa agattgtgaa aacattctca ttccagaaga aagactagcc tctttgaccg aagaagaaat tatcgaaaaa attcaaaaag atttctttgt tcaaaagtgt cgaaaagaaa tcaaaagttt atcaaaactt atttatggca attcagaaat gttgaacgaa catatggata tgattaatat aggttcaagc gcgggtgaac agaatgtcag atatgctgtt acggatttgc ctaaacctat tttaaagtct tcaggtacta aaatgttagg cagagcagat ccaaaatacc aacaaactga aaagaatgtt ttctatctcg atcaaaaaat taatagttat ataagcgccg ttctacattc taggaaacga attcttaaag agcattcatt agaacaagaa cgttgcggac aggtagtaaa aatgccttgc aaaacggtac aagatatttt aagtatgtca gggaatatta gtatagaaat cttaaattct gactttttgt tgtgcaaaca tctccaagat tttatgtgta aagttggatc tcgtctttca caaagcgagc gtaaagcaat tggtgataat aattataatg tacttgctga aagtatcggc atatcagtaa gtaaagcaaa gagagttacg caaattatta agcgagccca agaggtatgc cagaaagaaa aagtattcaa agctgatcgc tcgagaatta tggctacagg attttccaag taa
Details for BaclA.17330.b.B1.GE35676
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHML ARSDKIEEGL KIIDQKLSRK NNWRNLSREE FVHNASVTFA FLHNLQPFVA GNGCAQRIFF EKIAEAAGHK LDFSVVTEAR MQFAGSAALS FGDNEGNVAP LRHLFEDISN PEKVSVLKEF IQYTSDIKGE DIRDKVIVMP NEGETYTGFY KSCSLDSIML MVKDSYVLCK KDYLPPEKLK TLKLGDSITF TASISKDCEN ILIPEERLAS LTEEEIIEKI QKDFFVQKCR KEIKSLSKLI YGNSEMLNEH MDMINIGSSA GEQNVRYAVT DLPKPILKSS GTKMLGRADP KYQQTEKNVF YLDQKINSYI SAVLHSRKRI LKEHSLEQER CGQVVKMPCK TVQDILSMSG NISIEILNSD FLLCKHLQDF MCKVGSRLSQ SERKAIGDNN YNVLAESIGI SVSKAKRVTQ IIKRAQEVCQ KEK
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gcaaggagtg ataaaattga agaaggtttg aaaataatcg accaaaagct ttccaggaag aataattggc ggaatttatc gcgtgaagaa tttgttcata acgcatcagt aacatttgca tttctccata atttacagcc ttttgtagct gggaatggat gcgcacaacg aatatttttt gaaaaaatcg ctgaggctgc aggtcataag cttgacttct cggttgtgac agaagcacgt atgcaatttg ccggtagtgc tgcgttatca ttcggtgata atgaaggcaa tgtagcccct ctgcgtcatc tttttgagga tatttccaat ccagaaaaag tgagtgtttt gaaagaattc atacaatata caagtgatat taaaggtgaa gatattagag ataaagttat tgtaatgcca aatgaaggcg aaacctatac gggattctat aaaagttgta gtctagattc tattatgctc atggtgaaag atagttatgt tttatgcaaa aaagattatc ttccaccaga aaaattaaag acattaaaac tcggtgatag tattaccttt acagcttcaa tatcaaaaga ttgtgaaaac attctcattc cagaagaaag actagcctct ttgaccgaag aagaaattat cgaaaaaatt caaaaagatt tctttgttca aaagtgtcga aaagaaatca aaagtttatc aaaacttatt tatggcaatt cagaaatgtt gaacgaacat atggatatga ttaatatagg ttcaagcgcg ggtgaacaga atgtcagata tgctgttacg gatttgccta aacctatttt aaagtcttca ggtactaaaa tgttaggcag agcagatcca aaataccaac aaactgaaaa gaatgttttc tatctcgatc aaaaaattaa tagttatata agcgccgttc tacattctag gaaacgaatt cttaaagagc attcattaga acaagaacgt tgcggacagg tagtaaaaat gccttgcaaa acggtacaag atattttaag tatgtcaggg aatattagta tagaaatctt aaattctgac tttttgttgt gcaaacatct ccaagatttt atgtgtaaag ttggatctcg tctttcacaa agcgagcgta aagcaattgg tgataataat tataatgtac ttgctgaaag tatcggcata tcagtaagta aagcaaagag agttacgcaa attattaagc gagcccaaga ggtatgccag aaagaaaaag t
Expected Protein Sequence
MAHHHHHHML ARSDKIEEGL KIIDQKLSRK NNWRNLSREE FVHNASVTFA FLHNLQPFVA GNGCAQRIFF EKIAEAAGHK LDFSVVTEAR MQFAGSAALS FGDNEGNVAP LRHLFEDISN PEKVSVLKEF IQYTSDIKGE DIRDKVIVMP NEGETYTGFY KSCSLDSIML MVKDSYVLCK KDYLPPEKLK TLKLGDSITF TASISKDCEN ILIPEERLAS LTEEEIIEKI QKDFFVQKCR KEIKSLSKLI YGNSEMLNEH MDMINIGSSA GEQNVRYAVT DLPKPILKSS GTKMLGRADP KYQQTEKNVF YLDQKINSYI SAVLHSRKRI LKEHSLEQER CGQVVKMPCK TVQDILSMSG NISIEILNSD FLLCKHLQDF MCKVGSRLSQ SERKAIGDNN YNVLAESIGI SVSKAKRVTQ IIKRAQEVCQ KEKVFKADRS RIMATGFSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg caaggagtga taaaattgaa gaaggtttga aaataatcga ccaaaagctt tccaggaaga ataattggcg gaatttatcg cgtgaagaat ttgttcataa cgcatcagta acatttgcat ttctccataa tttacagcct tttgtagctg ggaatggatg cgcacaacga atattttttg aaaaaatcgc tgaggctgca ggtcataagc ttgacttctc ggttgtgaca gaagcacgta tgcaatttgc cggtagtgct gcgttatcat tcggtgataa tgaaggcaat gtagcccctc tgcgtcatct ttttgaggat atttccaatc cagaaaaagt gagtgttttg aaagaattca tacaatatac aagtgatatt aaaggtgaag atattagaga taaagttatt gtaatgccaa atgaaggcga aacctatacg ggattctata aaagttgtag tctagattct attatgctca tggtgaaaga tagttatgtt ttatgcaaaa aagattatct tccaccagaa aaattaaaga cattaaaact cggtgatagt attaccttta cagcttcaat atcaaaagat tgtgaaaaca ttctcattcc agaagaaaga ctagcctctt tgaccgaaga agaaattatc gaaaaaattc aaaaagattt ctttgttcaa aagtgtcgaa aagaaatcaa aagtttatca aaacttattt atggcaattc agaaatgttg aacgaacata tggatatgat taatataggt tcaagcgcgg gtgaacagaa tgtcagatat gctgttacgg atttgcctaa acctatttta aagtcttcag gtactaaaat gttaggcaga gcagatccaa aataccaaca aactgaaaag aatgttttct atctcgatca aaaaattaat agttatataa gcgccgttct acattctagg aaacgaattc ttaaagagca ttcattagaa caagaacgtt gcggacaggt agtaaaaatg ccttgcaaaa cggtacaaga tattttaagt atgtcaggga atattagtat agaaatctta aattctgact ttttgttgtg caaacatctc caagatttta tgtgtaaagt tggatctcgt ctttcacaaa gcgagcgtaa agcaattggt gataataatt ataatgtact tgctgaaagt atcggcatat cagtaagtaa agcaaagaga gttacgcaaa ttattaagcg agcccaagag gtatgccaga aagaaaaagt attcaaagct gatcgctcga gaattatggc tacaggattt tccaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaclA.17330.b.B1.PS01656
PURIFICATION DATe: 10/20/2012
CONCENTRATION: 5.71mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML ARSDKIEEGL KIIDQKLSRK NNWRNLSREE FVHNASVTFA FLHNLQPFVA GNGCAQRIFF EKIAEAAGHK LDFSVVTEAR MQFAGSAALS FGDNEGNVAP LRHLFEDISN PEKVSVLKEF IQYTSDIKGE DIRDKVIVMP NEGETYTGFY KSCSLDSIML MVKDSYVLCK KDYLPPEKLK TLKLGDSITF TASISKDCEN ILIPEERLAS LTEEEIIEKI QKDFFVQKCR KEIKSLSKLI YGNSEMLNEH MDMINIGSSA GEQNVRYAVT DLPKPILKSS GTKMLGRADP KYQQTEKNVF YLDQKINSYI SAVLHSRKRI LKEHSLEQER CGQVVKMPCK TVQDILSMSG NISIEILNSD FLLCKHLQDF MCKVGSRLSQ SERKAIGDNN YNVLAESIGI SVSKAKRVTQ IIKRAQEVCQ KEK
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gcaaggagtg ataaaattga agaaggtttg aaaataatcg accaaaagct ttccaggaag aataattggc ggaatttatc gcgtgaagaa tttgttcata acgcatcagt aacatttgca tttctccata atttacagcc ttttgtagct gggaatggat gcgcacaacg aatatttttt gaaaaaatcg ctgaggctgc aggtcataag cttgacttct cggttgtgac agaagcacgt atgcaatttg ccggtagtgc tgcgttatca ttcggtgata atgaaggcaa tgtagcccct ctgcgtcatc tttttgagga tatttccaat ccagaaaaag tgagtgtttt gaaagaattc atacaatata caagtgatat taaaggtgaa gatattagag ataaagttat tgtaatgcca aatgaaggcg aaacctatac gggattctat aaaagttgta gtctagattc tattatgctc atggtgaaag atagttatgt tttatgcaaa aaagattatc ttccaccaga aaaattaaag acattaaaac tcggtgatag tattaccttt acagcttcaa tatcaaaaga ttgtgaaaac attctcattc cagaagaaag actagcctct ttgaccgaag aagaaattat cgaaaaaatt caaaaagatt tctttgttca aaagtgtcga aaagaaatca aaagtttatc aaaacttatt tatggcaatt cagaaatgtt gaacgaacat atggatatga ttaatatagg ttcaagcgcg ggtgaacaga atgtcagata tgctgttacg gatttgccta aacctatttt aaagtcttca ggtactaaaa tgttaggcag agcagatcca aaataccaac aaactgaaaa gaatgttttc tatctcgatc aaaaaattaa tagttatata agcgccgttc tacattctag gaaacgaatt cttaaagagc attcattaga acaagaacgt tgcggacagg tagtaaaaat gccttgcaaa acggtacaag atattttaag tatgtcaggg aatattagta tagaaatctt aaattctgac tttttgttgt gcaaacatct ccaagatttt atgtgtaaag ttggatctcg tctttcacaa agcgagcgta aagcaattgg tgataataat tataatgtac ttgctgaaag tatcggcata tcagtaagta aagcaaagag agttacgcaa attattaagc gagcccaaga ggtatgccag aaagaaaaag t
Expressed Protein Sequence
MAHHHHHHML ARSDKIEEGL KIIDQKLSRK NNWRNLSREE FVHNASVTFA FLHNLQPFVA GNGCAQRIFF EKIAEAAGHK LDFSVVTEAR MQFAGSAALS FGDNEGNVAP LRHLFEDISN PEKVSVLKEF IQYTSDIKGE DIRDKVIVMP NEGETYTGFY KSCSLDSIML MVKDSYVLCK KDYLPPEKLK TLKLGDSITF TASISKDCEN ILIPEERLAS LTEEEIIEKI QKDFFVQKCR KEIKSLSKLI YGNSEMLNEH MDMINIGSSA GEQNVRYAVT DLPKPILKSS GTKMLGRADP KYQQTEKNVF YLDQKINSYI SAVLHSRKRI LKEHSLEQER CGQVVKMPCK TVQDILSMSG NISIEILNSD FLLCKHLQDF MCKVGSRLSQ SERKAIGDNN YNVLAESIGI SVSKAKRVTQ IIKRAQEVCQ KEKVFKADRS RIMATGFSK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGG CAAGGAGTGA TAAAATTGAA GAAGGTTTGA AAATAATCGA CCAAAAGCTT TCCAGGAAGA ATAATTGGCG GAATTTATCG CGTGAAGAAT TTGTTCATAA CGCATCAGTA ACATTTGCAT TTCTCCATAA TTTACAGCCT TTTGTAGCTG GGAATGGATG CGCACAACGA ATATTTTTTG AAAAAATCGC TGAGGCTGCA GGTCATAAGC TTGACTTCTC GGTTGTGACA GAAGCACGTA TGCAATTTGC CGGTAGTGCT GCGTTATCAT TCGGTGATAA TGAAGGCAAT GTAGCCCCTC TGCGTCATCT TTTTGAGGAT ATTTCCAATC CAGAAAAAGT GAGTGTTTTG AAAGAATTCA TACAATATAC AAGTGATATT AAAGGTGAAG ATATTAGAGA TAAAGTTATT GTAATGCCAA ATGAAGGCGA AACCTATACG GGATTCTATA AAAGTTGTAG TCTAGATTCT ATTATGCTCA TGGTGAAAGA TAGTTATGTT TTATGCAAAA AAGATTATCT TCCACCAGAA AAATTAAAGA CATTAAAACT CGGTGATAGT ATTACCTTTA CAGCTTCAAT ATCAAAAGAT TGTGAAAACA TTCTCATTCC AGAAGAAAGA CTAGCCTCTT TGACCGAAGA AGAAATTATC GAAAAAATTC AAAAAGATTT CTTTGTTCAA AAGTGTCGAA AAGAAATCAA AAGTTTATCA AAACTTATTT ATGGCAATTC AGAAATGTTG AACGAACATA TGGATATGAT TAATATAGGT TCAAGCGCGG GTGAACAGAA TGTCAGATAT GCTGTTACGG ATTTGCCTAA ACCTATTTTA AAGTCTTCAG GTACTAAAAT GTTAGGCAGA GCAGATCCAA AATACCAACA AACTGAAAAG AATGTTTTCT ATCTCGATCA AAAAATTAAT AGTTATATAA GCGCCGTTCT ACATTCTAGG AAACGAATTC TTAAAGAGCA TTCATTAGAA CAAGAACGTT GCGGACAGGT AGTAAAAATG CCTTGCAAAA CGGTACAAGA TATTTTAAGT ATGTCAGGGA ATATTAGTAT AGAAATCTTA AATTCTGACT TTTTGTTGTG CAAACATCTC CAAGATTTTA TGTGTAAAGT TGGATCTCGT CTTTCACAAA GCGAGCGTAA AGCAATTGGT GATAATAATT ATAATGTACT TGCTGAAAGT ATCGGCATAT CAGTAAGTAA AGCAAAGAGA GTTACGCAAA TTATTAAGCG AGCCCAAGAG GTATGCCAGA AAGAAAAAGT ATTCAAAGCT GATCGCTCGA GAATTATGGC TACAGGATTT TCCAAGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T