BaclA.17330.a

Bep8 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaclA.17330.a
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	HSQC satisfactory
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.a.B1.GE35675	full length		1	544
BaclA.17330.a.B2.GE37010	FIC		10	230

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.17330.a.B1.PS01627	full length		1	544

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_171090
PATRIC ID:	fig\|696125.3.peg.595
RefSeq:	YP_004158892.1
UniProt:	E6YHH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTISSDISLA RNYAYPNSKT LKNKYGIMDF KKLEKKCTHD AKKVIVNLHQ EPLPEKFDSS YLKYLHKCLF EEAFEWAGHT RDLPFTFKDG TTAQMLMMKM PNSNIFFVAG NKIQESLQEF DQMLVAKNNL QGLSREEFID EAAKLFSSLN YIHPFRGGNG PTQRVFFEKL AEAAGHRIDF SVITKERMIR TCINAIMLKG DEAHEAMKHL FEDISNPEKV RVLKELFNHI PRLECKRLDN EYVVMPREGM TYTGIYKGGS LDSIIVETVD SCVICHKDYL TPEQIKALKF SDEITFTVPI NKDLDQILIP AEKLAPLREE EIIKKIENAT YVQKSRIKIE FLSKLVYGDP TILDKHMNLI NKDPEMGQQL AEQIRNSPKS ISKLEGFKIG LIRSPKREVA ERNISEFSKA IIEHVDIVQY TKRKILKEHQ KEKKRVEQVI KRPSQEVQKI LELQPNKWEE ALKTESPEVL HKDLRGFLNK INSRLSISER KNIDSADCET LAESIGISDS KAKIIIQAFH QGTELYKQLE IIKTDRSKVV TMAI

NT Sequence

atgacaatat cttccgatat atccttagcc cgtaattatg cctatcctaa cagtaaaaca ttaaagaata aatatggaat aatggatttt aaaaaacttg aaaagaaatg cacacatgat gcaaaaaaag taatcgtcaa tttgcatcaa gaaccgctac cagaaaagtt tgattcttct tatttaaaat atctccataa atgcttattt gaagaggcat ttgaatgggc cggacatacc cgcgatctcc cctttacatt taaagacggc acaactgccc aaatgttaat gatgaaaatg ccgaattcaa atattttttt tgtagctggt aacaaaattc aagaaagctt acaggaattt gaccaaatgc ttgttgcaaa gaataatttg caaggtttat cgcgcgaaga atttattgat gaggcagcaa agcttttttc ttctctcaac tatatacatc ctttcagagg tggtaatgga cctacacagc gagtattttt tgaaaagctt gcggaagctg caggtcatag aattgatttt tcagttataa caaaagagcg gatgatacgc acctgtatta atgcgataat gctaaagggt gatgaagctc atgaagcgat gaaacattta tttgaagata tttccaatcc agaaaaagta cgtgttttaa aggaattatt caatcacata cctaggcttg agtgcaaacg tttggataat gaatatgttg taatgccacg tgaaggtatg acctatacag gtatttataa aggaggtagt ttagattcta tcatagttga aacagtggat tcttgcgtca tatgtcataa agattatctt acaccagaac aaatcaaggc attaaaattc agtgacgaaa ttacctttac agttccaata aataaagatt tagatcaaat tcttatccca gcagaaaagt tagctccttt gagggaagag gaaataatca agaagattga aaatgctact tatgttcaaa aaagccgaat aaaaatcgag tttttatcaa agcttgttta tggagatcca acaatattag ataagcatat gaatcttatt aataaagatc ctgaaatggg tcaacagctt gccgaacaga ttagaaattc tcctaaatct atttctaagc ttgaaggttt caaaataggg cttataagga gtccaaaacg cgaggtagct gaaagaaata tttcagaatt tagtaaagca attatagaac acgtggatat tgtacaatat actaaaagga aaattcttaa agagcatcaa aaagaaaaaa aacgtgttga acaagtaata aaaaggccta gccaagaagt gcaaaagatt cttgaattgc aaccaaataa atgggaagaa gctttaaaga cagagagtcc tgaagtgctt cataaagatc tccgcggttt tttgaataaa atcaattctc gtctttctat aagcgagcgg aaaaatatcg attctgctga ttgtgaaaca ttggctgaaa gtattggcat atcagacagt aaagcaaaga taattataca agcttttcat caaggcacag aattatacaa acagcttgaa ataattaaaa ctgaccgctc gaaagtagtg actatggcca tctga

Details for BaclA.17330.a.B1.GE35675

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	63kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MDFKKLEKKC THDAKKVIVN LHQEPLPEKF DSSYLKYLHK CLFEEAFEWA GHTRDLPFTF KDGTTAQMLM MKMPNSNIFF VAGNKIQESL QEFDQMLVAK NNLQGLSREE FIDEAAKLFS SLNYIHPFRG GNGPTQRVFF EKLAEAAGHR IDFSVITKER MIRTCINAIM LKGDEAHEAM KHLFEDISNP EKVRVLKELF NHIPRLECKR LDNEYVVMPR EGMTYTGIYK GGSLDSIIVE TVXSCVICHK DYLTPEQIKA LKFSDEITFT VPINKDLDQI LIPAEKLAPX REEEXIKKIE NATYVQKSRI KIEFLSKLVY GDPTILDKHM NLINKDPEMG QQLAEQIRNS PKSISKLEGF KIGLIRSPKR EVAERNISEF SKAIIEHVDI VQYTKRKILK EHQKEKKRVE QVIKRPSQEV QKILELQPNK WEEALKTESP EVLHKDLRGF LNKINSRLSI SERKNIDSAD CETLAESIGI SDSKAKIIIQ AFHQGTELYK QLEIIKTDRS KVVTMAI

Validated NT Sequence

atcttactca cttagatggc catagtcact actttcgagc ggtcagtttt aattatttca agctgtttgt ataattctgt gccttgatga aaagcttgta taattatctt tgctttactg tctgatatgc caatactttc agccaatgtt tcacaatcag cagaatcgat atttttccgc tcgcttatag aaagacgaga attgatttta ttcaaaaaac cgcggagatc tttatgaagc acttcaggac tctctgtctt taaagcttct tcccatttat ttggttgcaa ttcaagaatc ttttgcactt cttggctagg cctttttatt acttgttcaa cacgtttttt ttctttttga tgctctttaa gaattttcct tttagtatat tgtacaatat ccacgtgttc tataattgct ttactaaatt ctgaaatatt tctttcagct acctcgcgtt ttggactcct tataagccct attttgaaac cttcaagctt agaaatagat ttaggagaat ttctaatctg ttcggcaagc tgttgaccca tttcaggatc tttattaata agattcatat gcttatctaa tattgttgga tctccataaa caagctttga taaaaactcg atttttattc ggcttttttg aacataagta gcattttcaa tcttcttgat tanttcctct tccctnnaag gagctaactt ttctgctggg ataagaattt gatctaaatc tttatttatt ggaactgtaa aggtaatttc gtcactgaat tttaatgcct tgatttgttc tggtgtaaga taatctttat gacatatgac gcaagaatnc actgtttcaa ctatgataga atctaaacta cctcctttat aaatacctgt ataggtcata ccttcacgtg gcattacaac atattcatta tccaaacgtt tgcactcaag cctaggtatg tgattgaata attcctttaa aacacgtact ttttctggat tggaaatatc ttcaaataaa tgtttcatcg cttcatgagc ttcatcaccc tttagcatta tcgcattaat acaggtgcgt atcatccgct cttttgttat aactgaaaaa tcaattctat gacctgcagc ttccgcaagc ttttcaaaaa atactcgctg tgtaggtcca ttaccacctc tgaaaggatg tatatagttg agagaagaaa aaagctttgc tgcctcatca ataaattctt cgcgcgataa accttgcaaa ttattctttg caacaagcat ttggtcaaat tcctgtaagc tttcttgaat tttgttacca gctacaaaaa aaatatttga attcggcatt ttcatcatta acatttgggc agttgtgccg tctttaaatg taaaggggag atcgcgggta tgtccggccc attcaaatgc ctcttcaaat aagcatttat ggagatattt taaataagaa gaatcaaact tttctggtag cggttcttga tgcaaattga cgattacttt ttttgcatca tgtgtgcatt tcttttcaag ttttttaaaa tccattattc catatttatt ctttaatgtt ttactgttag gataggcata attacgggct aaggatatat cggaa

Expected Protein Sequence

MAHHHHHHMT ISSDISLARN YAYPNSKTLK NKYGIMDFKK LEKKCTHDAK KVIVNLHQEP LPEKFDSSYL KYLHKCLFEE AFEWAGHTRD LPFTFKDGTT AQMLMMKMPN SNIFFVAGNK IQESLQEFDQ MLVAKNNLQG LSREEFIDEA AKLFSSLNYI HPFRGGNGPT QRVFFEKLAE AAGHRIDFSV ITKERMIRTC INAIMLKGDE AHEAMKHLFE DISNPEKVRV LKELFNHIPR LECKRLDNEY VVMPREGMTY TGIYKGGSLD SIIVETVDSC VICHKDYLTP EQIKALKFSD EITFTVPINK DLDQILIPAE KLAPLREEEI IKKIENATYV QKSRIKIEFL SKLVYGDPTI LDKHMNLINK DPEMGQQLAE QIRNSPKSIS KLEGFKIGLI RSPKREVAER NISEFSKAII EHVDIVQYTK RKILKEHQKE KKRVEQVIKR PSQEVQKILE LQPNKWEEAL KTESPEVLHK DLRGFLNKIN SRLSISERKN IDSADCETLA ESIGISDSKA KIIIQAFHQG TELYKQLEII KTDRSKVVTM AI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa tatcttccga tatatcctta gcccgtaatt atgcctatcc taacagtaaa acattaaaga ataaatatgg aataatggat tttaaaaaac ttgaaaagaa atgcacacat gatgcaaaaa aagtaatcgt caatttgcat caagaaccgc taccagaaaa gtttgattct tcttatttaa aatatctcca taaatgctta tttgaagagg catttgaatg ggccggacat acccgcgatc tcccctttac atttaaagac ggcacaactg cccaaatgtt aatgatgaaa atgccgaatt caaatatttt ttttgtagct ggtaacaaaa ttcaagaaag cttacaggaa tttgaccaaa tgcttgttgc aaagaataat ttgcaaggtt tatcgcgcga agaatttatt gatgaggcag caaagctttt ttcttctctc aactatatac atcctttcag aggtggtaat ggacctacac agcgagtatt ttttgaaaag cttgcggaag ctgcaggtca tagaattgat ttttcagtta taacaaaaga gcggatgata cgcacctgta ttaatgcgat aatgctaaag ggtgatgaag ctcatgaagc gatgaaacat ttatttgaag atatttccaa tccagaaaaa gtacgtgttt taaaggaatt attcaatcac atacctaggc ttgagtgcaa acgtttggat aatgaatatg ttgtaatgcc acgtgaaggt atgacctata caggtattta taaaggaggt agtttagatt ctatcatagt tgaaacagtg gattcttgcg tcatatgtca taaagattat cttacaccag aacaaatcaa ggcattaaaa ttcagtgacg aaattacctt tacagttcca ataaataaag atttagatca aattcttatc ccagcagaaa agttagctcc tttgagggaa gaggaaataa tcaagaagat tgaaaatgct acttatgttc aaaaaagccg aataaaaatc gagtttttat caaagcttgt ttatggagat ccaacaatat tagataagca tatgaatctt attaataaag atcctgaaat gggtcaacag cttgccgaac agattagaaa ttctcctaaa tctatttcta agcttgaagg tttcaaaata gggcttataa ggagtccaaa acgcgaggta gctgaaagaa atatttcaga atttagtaaa gcaattatag aacacgtgga tattgtacaa tatactaaaa ggaaaattct taaagagcat caaaaagaaa aaaaacgtgt tgaacaagta ataaaaaggc ctagccaaga agtgcaaaag attcttgaat tgcaaccaaa taaatgggaa gaagctttaa agacagagag tcctgaagtg cttcataaag atctccgcgg ttttttgaat aaaatcaatt ctcgtctttc tataagcgag cggaaaaata tcgattctgc tgattgtgaa acattggctg aaagtattgg catatcagac agtaaagcaa agataattat acaagctttt catcaaggca cagaattata caaacagctt gaaataatta aaactgaccg ctcgaaagta gtgactatgg ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BaclA.17330.a.B2.GE37010

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	27kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA RNYAYPNSKT LKNKYGIMDF KKLEKKCTHD AKKVIVNLHQ EPLPEKFDSS YLKYLHKCLF EEAFEWAGHT RDLPFTFKDG TTAQMLMMKM PNSNIFFVAG NKIQESLQEF DQMLVAKNNL QGLSREEFID EAAKLFSSLN YIHPFRGGNG PTQRVFFEKL AEAAGHRIDF SVITKERMIR TCINAIMLKG DEAHEAMKHL FEDISNPEKV RVLKELFNHI

Validated NT Sequence

atcttactca cttatatgtg attgaataat tcctttaaaa cacgtacttt ttctggattg gaaatatctt caaataaatg tttcatcgct tcatgagctt catcaccctt tagcattatc gcattaatac aggtgcgtat catccgctct tttgttataa ctgaaaaatc aattctatga cctgcagctt ccgcaagctt ttcaaaaaat actcgctgtg taggtccatt accacctctg aaaggatgta tatagttgag agaagaaaaa agctttgctg cctcatcaat aaattcttcg cgcgataaac cttgcaaatt attctttgca acaagcattt ggtcaaattc ctgtaagctt tcttgaattt tgttaccagc tacaaaaaaa atatttgaat tcggcatttt catcattaac atttgggcag ttgtgccgtc tttaaatgta aaggggagat cgcgggtatg tccggcccat tcaaatgcct cttcaaataa gcatttatgg agatatttta aataagaaga atcaaacttt tctggtagcg gttcttgatg caaattgacg attacttttt ttgcatcatg tgtgcatttc ttttcaagtt ttttaaaatc cattattcca tatttattct ttaatgtttt actgttagga taggcataat tacgggccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHAR NYAYPNSKTL KNKYGIMDFK KLEKKCTHDA KKVIVNLHQE PLPEKFDSSY LKYLHKCLFE EAFEWAGHTR DLPFTFKDGT TAQMLMMKMP NSNIFFVAGN KIQESLQEFD QMLVAKNNLQ GLSREEFIDE AAKLFSSLNY IHPFRGGNGP TQRVFFEKLA EAAGHRIDFS VITKERMIRT CINAIMLKGD EAHEAMKHLF EDISNPEKVR VLKELFNHI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcccgta attatgccta tcctaacagt aaaacattaa agaataaata tggaataatg gattttaaaa aacttgaaaa gaaatgcaca catgatgcaa aaaaagtaat cgtcaatttg catcaagaac cgctaccaga aaagtttgat tcttcttatt taaaatatct ccataaatgc ttatttgaag aggcatttga atgggccgga catacccgcg atctcccctt tacatttaaa gacggcacaa ctgcccaaat gttaatgatg aaaatgccga attcaaatat tttttttgta gctggtaaca aaattcaaga aagcttacag gaatttgacc aaatgcttgt tgcaaagaat aatttgcaag gtttatcgcg cgaagaattt attgatgagg cagcaaagct tttttcttct ctcaactata tacatccttt cagaggtggt aatggaccta cacagcgagt attttttgaa aagcttgcgg aagctgcagg tcatagaatt gatttttcag ttataacaaa agagcggatg atacgcacct gtattaatgc gataatgcta aagggtgatg aagctcatga agcgatgaaa catttatttg aagatatttc caatccagaa aaagtacgtg ttttaaagga attattcaat cacatatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BaclA.17330.a.B1.PS01627

PURIFICATION DATe:	9/20/2012
CONCENTRATION:	19.6mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA TATCTTCCGA TATATCCTTA GCCCGTAATT ATGCCTATCC TAACAGTAAA ACATTAAAGA ATAAATATGG AATAATGGAT TTTAAAAAAC TTGAAAAGAA ATGCACACAT GATGCAAAAA AAGTAATCGT CAATTTGCAT CAAGAACCGC TACCAGAAAA GTTTGATTCT TCTTATTTAA AATATCTCCA TAAATGCTTA TTTGAAGAGG CATTTGAATG GGCCGGACAT ACCCGCGATC TCCCCTTTAC ATTTAAAGAC GGCACAACTG CCCAAATGTT AATGATGAAA ATGCCGAATT CAAATATTTT TTTTGTAGCT GGTAACAAAA TTCAAGAAAG CTTACAGGAA TTTGACCAAA TGCTTGTTGC AAAGAATAAT TTGCAAGGTT TATCGCGCGA AGAATTTATT GATGAGGCAG CAAAGCTTTT TTCTTCTCTC AACTATATAC ATCCTTTCAG AGGTGGTAAT GGACCTACAC AGCGAGTATT TTTTGAAAAG CTTGCGGAAG CTGCAGGTCA TAGAATTGAT TTTTCAGTTA TAACAAAAGA GCGGATGATA CGCACCTGTA TTAATGCGAT AATGCTAAAG GGTGATGAAG CTCATGAAGC GATGAAACAT TTATTTGAAG ATATTTCCAA TCCAGAAAAA GTACGTGTTT TAAAGGAATT ATTCAATCAC ATACCTAGGC TTGAGTGCAA ACGTTTGGAT AATGAATATG TTGTAATGCC ACGTGAAGGT ATGACCTATA CAGGTATTTA TAAAGGAGGT AGTTTAGATT CTATCATAGT TGAAACAGTG GATTCTTGCG TCATATGTCA TAAAGATTAT CTTACACCAG AACAAATCAA GGCATTAAAA TTCAGTGACG AAATTACCTT TACAGTTCCA ATAAATAAAG ATTTAGATCA AATTCTTATC CCAGCAGAAA AGTTAGCTCC TTTGAGGGAA GAGGAAATAA TCAAGAAGAT TGAAAATGCT ACTTATGTTC AAAAAAGCCG AATAAAAATC GAGTTTTTAT CAAAGCTTGT TTATGGAGAT CCAACAATAT TAGATAAGCA TATGAATCTT ATTAATAAAG ATCCTGAAAT GGGTCAACAG CTTGCCGAAC AGATTAGAAA TTCTCCTAAA TCTATTTCTA AGCTTGAAGG TTTCAAAATA GGGCTTATAA GGAGTCCAAA ACGCGAGGTA GCTGAAAGAA ATATTTCAGA ATTTAGTAAA GCAATTATAG AACACGTGGA TATTGTACAA TATACTAAAA GGAAAATTCT TAAAGAGCAT CAAAAAGAAA AAAAACGTGT TGAACAAGTA ATAAAAAGGC CTAGCCAAGA AGTGCAAAAG ATTCTTGAAT TGCAACCAAA TAAATGGGAA GAAGCTTTAA AGACAGAGAG TCCTGAAGTG CTTCATAAAG ATCTCCGCGG TTTTTTGAAT AAAATCAATT CTCGTCTTTC TATAAGCGAG CGGAAAAATA TCGATTCTGC TGATTGTGAA ACATTGGCTG AAAGTATTGG CATATCAGAC AGTAAAGCAA AGATAATTAT ACAAGCTTTT CATCAAGGCA CAGAATTATA CAAACAGCTT GAAATAATTA AAACTGACCG CTCGAAAGTA GTGACTATGG CCATCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT