SSGCID: Target Page

BaclA.01664.a

Two-component sensor kinase BatS (EC 2.7.3.-) (BatR-BatS two-component system)

CENTER ID:	BaclA.01664.a
ORGANISM:	Bartonella clarridgeiae CIP 104772 / 73
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.01664.a.B2.GE35921	kinase domain		329	590
BaclA.01664.a.B3.GE37249	periplasmic domain		64	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaclA.01664.a.B2.PS01682	kinase domain		329	590

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|696125.3.peg.58
RefSeq:	YP_004158339.1
UniProt:	E6YFV3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKNTLLNKS SSNTSPIRFS MFVHLYFRIQ RLLRQLFFSS LTRRIVILNL AALGILVTSI LYLNQFRNGL IEAKIESLRT QGKIIAGAIA ASATIDTNSI LIDPQKLLEL QAGESVTPTL QSTDTWDFPI NPEQVAPLLR RLIAPKTTRA RIYDRDATLL LDSRVLYSSG EVFTYNLPPI QMKQNLWEHL KSFFFNLLYS NGITVDRRQV KDNETIHPEV HLALNGSSAT AKRRNRQGQL IVSVAVPVQR YRAVVGALLL STTGSDIDDI VESEKLIIFK IFAVVGSVFL VLSIFLAHTI AHPLSKLSSS ANRVRHGRSK RIKIPDFSKR EDEIGRLSTS ISDMTNALYT RIEAIERFAA DVSHELKNPL TSLRSAVETL PLTKNKAQQT QLLEIIQHDV HRLDRLITDI SDASRLDAEL ARETAKIVDM SSLLKSLIQA IREVHCNKQN IDINFNIIPR TNGKPYLIVG HELRLGQVIS NLIENARSFV PRKNGKICIT MKNNASNLIL TVEDNGPGIR SENIERIFER FYTDRPSKDA FGQHSGLGLA ISQQIIEAHN GTLTAENIVD PKFKKEKTGA RFIITLPLAK

NT Sequence

atgaaaaaaa atactctact caacaaatca tcaagcaata cttctccgat acgattttct atgtttgtac atttgtattt tcggatacaa cgtcttttaa ggcaattgtt cttttccagt ctcacacgcc gtattgttat tttaaatctt gctgcccttg gcattttagt tacaagtatt ttgtacctta atcaatttcg caatggtttg atcgaagcga aaattgaaag tttgcgcacc caaggaaaaa ttattgctgg agctattgcc gcatctgcaa caatagatac gaattctatt ttaattgacc cccaaaaact tttggaatta caggctggag aaagtgttac accaacactt caatcaactg atacttggga tttccccatt aatccggaac aagttgcccc acttttgcga cgtcttattg caccaaaaac gacaagagca cggatatatg atcgggatgc aactttgctt cttgattcac gtgttcttta ttcgagtggt gaagttttta cctataattt acctcctatt caaatgaaac aaaatctttg ggagcactta aaatcctttt ttttcaatct actctatagc aacggtatta ctgttgatag aagacaagta aaagataatg agactatcca cccagaagtt catctagcat taaatggctc ttctgccact gctaaacgac gtaacaggca aggtcaatta attgtttcgg ttgctgtgcc tgttcaacgt tatcgtgcag tagtcggcgc tcttttgctt tcaactaccg gttcagacat tgatgatatt gtagaaagtg aaaaattgat catttttaaa atctttgccg ttgttggaag tgtgtttttg gttctttcta tatttttagc ccatactatt gctcatccat taagtaaatt atcttcctct gctaatcgtg tgcgccatgg tcgtagtaag cgaataaaaa ttcctgattt ttcaaaacgt gaagatgaaa ttggtcgtct ttccacctct attagcgata tgaccaacgc tctttataca cgcattgaag ctattgaacg ttttgcagct gatgtaagcc atgaattaaa gaaccctctc acctcgctac gaagtgccgt tgaaacacta ccactaacta aaaataaagc acaacagaca caattgcttg aaattatcca acatgatgtg catcgtcttg accgtttaat taccgatatt tctgatgcgt cgcggttaga tgcagaactt gcccgcgaaa ccgccaaaat agttgatatg agctcactat tgaaaagtct tattcaagct attcgtgaag tccattgtaa taaacaaaat attgatatta attttaatat tatcccacga actaatggta aaccctatct tattgtagga catgaattac gtttaggtca ggtgatttct aatctcattg aaaatgcacg ttcctttgtt ccccgtaaaa atggtaaaat ttgtattacg atgaaaaata atgcttcaaa tcttatttta actgttgaag acaatggtcc tggtattcgt tcagaaaata tcgagcgtat ttttgaacgt ttctatactg atcgaccaag taaggatgct tttggacaac attcaggact tggccttgca attagtcagc aaatcattga agctcataat ggaactctca cagctgaaaa tattgttgac cccaaattta aaaaagaaaa aactggtgct cgatttatta tcactcttcc gcttgccaaa taa

Details for BaclA.01664.a.B2.GE35921

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MKREDEIGRL STSISDMTNA LYTRIEAIER FAADVSHELK NPLTSLRSAV ETLPLTKNKA QQTQLLEIIQ HDVHRLDRLI TDISDASRLD AELARETAKI VDMSSLLKSL IQAIREVHCN KQNIDINFNI IPRTNGKPYL IVGHELRLGQ VISNLIENAR SFVPRKNGKI CITMKNNASN LILTVEDNGP GIRSENIERI FERFYTDRPS KDAFGQHSGL GLAISQQIIE AHNGTLTAEN IVDPKFKKEK TGARFIITLP LAK

Validated NT Sequence

atcttactca cttatttggc aagcggaaga gtgataataa atcgagcacc agttttttct tttttaaatt tggggtcaac aatattttca gctgtgagag ttccattatg agcttcaatg atttgctgac taattgcaag gccaagtcct gaatgttgtc caaaagcatc cttacttggt cgatcagtat agaaacgttc aaaaatacgc tcgatatttt ctgaacgaat accaggacca ttgtcttcaa cagttaaaat aagatttgaa gcattatttt tcatcgtaat acaaatttta ccatttttac ggggaacaaa ggaacgtgca ttttcaatga gattagaaat cacctgacct aaacgtaatt catgtcctac aataagatag ggtttaccat tagttcgtgg gataatatta aaattaatat caatattttg tttattacaa tggacttcac gaatagcttg aataagactt ttcaatagtg agctcatatc aactattttg gcggtttcgc gggcaagttc tgcatctaac cgcgacgcat cagaaatatc ggtaattaaa cggtcaagac gatgcacatc atgttggata atttcaagca attgtgtctg ttgtgcttta tttttagtta gtggtagtgt ttcaacggca cttcgtagcg aggtgagagg gttctttaat tcatggctta catcagctgc aaaacgttca atagcttcaa tgcgtgtata aagagcgttg gtcatatcgc taatagaggt ggaaagacga ccaatttcat cttcacgttt catatggtgg tggtggtggt ga

Expected Protein Sequence

MAHHHHHHMK REDEIGRLST SISDMTNALY TRIEAIERFA ADVSHELKNP LTSLRSAVET LPLTKNKAQQ TQLLEIIQHD VHRLDRLITD ISDASRLDAE LARETAKIVD MSSLLKSLIQ AIREVHCNKQ NIDINFNIIP RTNGKPYLIV GHELRLGQVI SNLIENARSF VPRKNGKICI TMKNNASNLI LTVEDNGPGI RSENIERIFE RFYTDRPSKD AFGQHSGLGL AISQQIIEAH NGTLTAENIV DPKFKKEKTG ARFIITLPLA K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gtgaagatga aattggtcgt ctttccacct ctattagcga tatgaccaac gctctttata cacgcattga agctattgaa cgttttgcag ctgatgtaag ccatgaatta aagaaccctc tcacctcgct acgaagtgcc gttgaaacac taccactaac taaaaataaa gcacaacaga cacaattgct tgaaattatc caacatgatg tgcatcgtct tgaccgttta attaccgata tttctgatgc gtcgcggtta gatgcagaac ttgcccgcga aaccgccaaa atagttgata tgagctcact attgaaaagt cttattcaag ctattcgtga agtccattgt aataaacaaa atattgatat taattttaat attatcccac gaactaatgg taaaccctat cttattgtag gacatgaatt acgtttaggt caggtgattt ctaatctcat tgaaaatgca cgttcctttg ttccccgtaa aaatggtaaa atttgtatta cgatgaaaaa taatgcttca aatcttattt taactgttga agacaatggt cctggtattc gttcagaaaa tatcgagcgt atttttgaac gtttctatac tgatcgacca agtaaggatg cttttggaca acattcagga cttggccttg caattagtca gcaaatcatt gaagctcata atggaactct cacagctgaa aatattgttg accccaaatt taaaaaagaa aaaactggtg ctcgatttat tatcactctt ccgcttgcca aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BaclA.01664.a.B3.GE37249

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRNGLIEAK IESLRTQGKI IAGAIAASAT IDTNSILIDP QKLLELQAGE SVTPTLQSTD TWDFPINPEQ VAPLLRRLIA PKTTRARIYD RDATLLLDSR VLYSSGEVFT YNLPPIQMKQ NLWEHLKSFF FNLLYSNGIT VDRRQVKDNE TIHPEVHLAL NGSSATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTTG SDIDDIVESE K

Validated NT Sequence

ttntnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgaatcaa tttcgcaatg gtttgatcga agcgaaaatt gaaagtttgc gcacccaagg aaaaattatt gctggagcta ttgccgcatc tgcaacaata gatacgaatt ctattttaat tgacccccaa aaacttttgg aattacaggc tggagaaagt gttacaccaa cacttcaatc aactgatact tgggatttcc ccattaatcc ggaacaagtt gccccacttt tgcgacgtct tattgcacca aaaacgacaa gagcacggat atatgatcgg gatgcaactt tgcttcttga ttcacgtgtt ctttattcga gtggtgaagt ttttacctat aatttacctc ctattcaaat gaaacaaaat ctttgggagc acttaaaatc cttttttttc aatctactct atagcaacgg tattactgtt gatagaagac aagtaaaaga taatgagact atccacccag aagttcatct agcattaaat ggctcttctg ccactgctaa acgacgtaac aggcaaggtc aattaattgt ttcggttgct gtgcctgttc aacgttatcg tgcagtagtc ggcgctcttt tgctttcaac taccggttca gacattgatg atattgtaga aagtgaaaaa taagtgagta agataggatc cggctgnnnn annncccccn aannnnnnnn nnnnnnnnaa annnnnnnng gaccgncaat gnnnnngccn atttgctgcn naacttctgg tcnnaatgcc ntgatctttt gcnnaccnga cggggccnan aanttcaccn tgacnganat gganaacnan ncannnnngn nacctgcnnc nnngcngggc agcncnantt caacnccnna ccacnangcn ntgangnntg nnnnccnggn gncatcnnat cgngcnnnna tctnnccnna tcngccnann nnnnnggnng nncnannncn cgnngcnnnt nccggnnann nngnnnnnnn nacnacnncc nnnnnnnncc gnnnnnannn tnnnnnnnta cnnnnnnnnt gnnnnnnnnn nnnnnaannn nnnnnntnnn nnncnncnnn nnnnnnnnan nnnnncnnnn ac

Expected Protein Sequence

MAHHHHHHNQ FRNGLIEAKI ESLRTQGKII AGAIAASATI DTNSILIDPQ KLLELQAGES VTPTLQSTDT WDFPINPEQV APLLRRLIAP KTTRARIYDR DATLLLDSRV LYSSGEVFTY NLPPIQMKQN LWEHLKSFFF NLLYSNGITV DRRQVKDNET IHPEVHLALN GSSATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTTGS DIDDIVESEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcaat ttcgcaatgg tttgatcgaa gcgaaaattg aaagtttgcg cacccaagga aaaattattg ctggagctat tgccgcatct gcaacaatag atacgaattc tattttaatt gacccccaaa aacttttgga attacaggct ggagaaagtg ttacaccaac acttcaatca actgatactt gggatttccc cattaatccg gaacaagttg ccccactttt gcgacgtctt attgcaccaa aaacgacaag agcacggata tatgatcggg atgcaacttt gcttcttgat tcacgtgttc tttattcgag tggtgaagtt tttacctata atttacctcc tattcaaatg aaacaaaatc tttgggagca cttaaaatcc ttttttttca atctactcta tagcaacggt attactgttg atagaagaca agtaaaagat aatgagacta tccacccaga agttcatcta gcattaaatg gctcttctgc cactgctaaa cgacgtaaca ggcaaggtca attaattgtt tcggttgctg tgcctgttca acgttatcgt gcagtagtcg gcgctctttt gctttcaact accggttcag acattgatga tattgtagaa agtgaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaclA.01664.a.B2.PS01682

PURIFICATION DATe:	11/8/2012
CONCENTRATION:	90.5mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC GTGAAGATGA AATTGGTCGT CTTTCCACCT CTATTAGCGA TATGACCAAC GCTCTTTATA CACGCATTGA AGCTATTGAA CGTTTTGCAG CTGATGTAAG CCATGAATTA AAGAACCCTC TCACCTCGCT ACGAAGTGCC GTTGAAACAC TACCACTAAC TAAAAATAAA GCACAACAGA CACAATTGCT TGAAATTATC CAACATGATG TGCATCGTCT TGACCGTTTA ATTACCGATA TTTCTGATGC GTCGCGGTTA GATGCAGAAC TTGCCCGCGA AACCGCCAAA ATAGTTGATA TGAGCTCACT ATTGAAAAGT CTTATTCAAG CTATTCGTGA AGTCCATTGT AATAAACAAA ATATTGATAT TAATTTTAAT ATTATCCCAC GAACTAATGG TAAACCCTAT CTTATTGTAG GACATGAATT ACGTTTAGGT CAGGTGATTT CTAATCTCAT TGAAAATGCA CGTTCCTTTG TTCCCCGTAA AAATGGTAAA ATTTGTATTA CGATGAAAAA TAATGCTTCA AATCTTATTT TAACTGTTGA AGACAATGGT CCTGGTATTC GTTCAGAAAA TATCGAGCGT ATTTTTGAAC GTTTCTATAC TGATCGACCA AGTAAGGATG CTTTTGGACA ACATTCAGGA CTTGGCCTTG CAATTAGTCA GCAAATCATT GAAGCTCATA ATGGAACTCT CACAGCTGAA AATATTGTTG ACCCCAAATT TAAAAAAGAA AAAACTGGTG CTCGATTTAT TATCACTCTT CCGCTTGCCA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT