SSGCID: Target Page

BaboA.18343.a

GTPase of unknown function, putative

CENTER ID:	BaboA.18343.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.18343.a.A2.GE34062	mature protein		20	629

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_II004000
RefSeq:	XP_001609923.1
UniProt:	A7ATU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRIFALFYT IHFLLVLSSS FVLDSRKSCG FLGSHIHRHH LYDNLETVGS RDQLGTTENP PDDYYGLNSS DDHYGGANSV DSVGGTAELS GSDSAATSNK DAGSIMIPYS VNYPVHHEEL RGVVADERHE KAKEKLEHLG FFSPLASETA HLNTRQRDIS GPSDSHSLTM STLEPESRIW ECMKSSSLLQ QIGTKQASVQ ELTLQSPSRM DYESIRDQWV IGRARIHWFP RYFGKSLGEL SEYIKMCDVI LDVRDARIPY VADSDFLLNV YNNMFTHKPK IIVFTHADLS SINGSDEWAT YYRIKNFWEA QAFNRNIPDP NKKPVTPVIF VNARDGISCI VRLKKLIYKL CHRANQKRLR RGLAARPLRA IALGMPNVGK SALINRLLGR HKARSFNAAG VTRDIRLVRL HPDEYREAAH KIIDVIDTPG VLPANLYNMA FGPVGNKASR KRNRLLGSIY FKLPRTSVQG GHSKSGIDAI HTGLERNVWL IAAANQIVEG AYDNDQAAEN LLEQALHVYR SKRSYVDLPR ILKRIGIDYT PSILEMHPED FSHECLQVAS YKHHGGDLNA AASRILSDFR RGYYGRITLQ LPPDTVSNRY SVPFNGIEEP KPSSTHANRE YSQGLYEGW

NT Sequence

atgagtagga tatttgcttt gttttacaca atccactttc ttttggtgtt gagcagcagt ttcgtgttgg acagtaggaa atcgtgcggg tttttaggca gtcacattca ccgtcatcat ttgtatgaca acctggaaac tgttggttca cgtgaccagc tgggtaccac tgaaaatccc ccggatgatt attatgggct aaatagctcg gatgatcact atggcggagc aaatagcgtt gattcggttg gtggtacagc tgaactttcg ggatctgata gcgctgccac atcaaacaag gatgcgggta gcattatgat cccttactct gtgaactatc cagtccatca tgaggagctt cgtggtgttg ttgctgacga acgccatgaa aaggctaaag agaaattgga gcatttaggt ttcttttcac ctcttgccag cgaaactgca catttgaaca ctaggcagcg tgacatatcg ggaccttccg acagtcactc tttgacgatg tcaactttgg agcctgagag ccgcatatgg gagtgtatga agtcatcttc attgttacag caaattggta ctaagcaggc atcggttcag gaattgacat tacaatctcc tagcaggatg gattacgaga gcatacgtga ccagtgggtt ataggcaggg ctcgcattca ttggtttcca cgttactttg gtaagagtct gggtgagctc tctgagtaca tcaagatgtg tgatgttatt cttgacgttc gcgatgcgag gatcccttat gttgcggatt ccgactttct attgaatgtc tataacaaca tgtttactca caagcctaag ataatcgttt ttactcatgc tgatttaagt tctatcaatg gatctgacga atgggccact tattaccgca ttaagaactt ttgggaagcc caggcgttca ataggaacat tcctgatcct aacaagaagc cggtgacccc tgtgatattt gtcaatgctc gtgacggaat ttcgtgtata gttcgcttaa agaagttgat atacaagtta tgccaccgtg caaatcaaaa acgcttgcgt cgtggtttag ctgcacgccc actgcgtgca attgctttgg gcatgcccaa tgttggcaag agtgcgctta ttaaccgtct tcttggtcgt cacaaagctc gtagtttcaa tgcagcgggt gtaactcgtg acatacgact cgtccgtcta caccctgatg agtacaggga ggcagcccac aagattatag atgtgataga cactcctgga gtgctgcctg ctaatttgta taacatggcc tttgggccag ttggtaacaa ggccagtcgt aagcggaata ggttactggg cagtatatat tttaagctac cgcgcacgag tgttcagggt ggccacagca aaagcggtat tgatgcgatc catacaggtt tagagcgcaa tgtatggctc atagctgctg ctaaccaaat agttgagggt gcttatgaca acgaccaggc agcggaaaac ttgcttgagc aggctttgca tgtatatagg tccaagcgat cgtatgtaga tttaccgcgt atattgaagc gtattggcat tgactatacc cctagcatac tggagatgca tccagaggat tttagccacg agtgcctgca ggttgctagt tataaacatc acggtggtga cctcaatgct gcagccagcc gtatattgtc tgactttagg cgtggttact atggtcgcat aactttgcaa ctaccccctg atactgtgtc taaccgttat tcagtaccat tcaacggtat cgaggaaccc aaaccaagtt ctactcacgc caatcgtgaa tactcccaag gtttatacga gggatggtaa

Details for BaboA.18343.a.A2.GE34062

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	50

Validated AA Sequence

Validated NT Sequence

tttntttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagtttc gtgttggaca gtaggaaatc gtgcgggttt ttaggcagtc acattcaccg tcatcatttg tatgacaacc tggaaactgt tggttcacgt gaccagctgg gtaccactga aaatcccccg gatgattatt atgggctaaa tagctcggat gatcactatg gcggagcaaa tagcgttgat tcggttggtg gtacagctga actttcggga tctgatagcg ctgccacatc aaacaaggat gcgggtagca ttatgatccc ttactctgtg aactatccag tccatcatga ggagcttcgt ggtgttgttg ctgacgaacg ccatgaaaag gctaaagaga aattggagca tttaggtttc ttttcacctc ttgccagcga aactgcacat ttgaacacta ggcagcgtga catatcggga ccttccgaca gtcactcttt gacgatgtca actttggagc ctgagagccg catatgggag tgtatgaagt catcttcatt gttacagcaa attggtacta agcaggcatc ggttcaggaa ttgacattac aatctcctag caggatggat tacgagagca tacgtgacca gtgggttata ggcagggctc gcattcattg gtttccacgt tactttggta agagtctggg tgagctctct gantacatca agatgtgtga tgttattctt gacgttcgcg atgcgaggat cccttatgtt gcggantccg actttctatt gaatgtctat aacaacatgt ttactcacaa gcctaagata atcgttttta ctcntgctga tttaagttct atcaatggat ctgacgaatg ggccacttat taccgcattn agaacttttg ggaagcccnn ngttcannac nttnctgann ctancagaag cngnngaccc ngngannntt ngtcaatgct cnngannnan ttcnngnnnn nttcgcttaa annantnnnn nnnnntnntn cnnncnnnnn antcaaaaan nnnnnnnncn nnnnnnncng nnnnccnann gnnnnnnnnn nnnnngnnnn nnnnnnnnnn anannnnnnn nnnacnnntn nnnnnnnnnn nnnaannnnn nnnnanntnn nnnnnnnnnn nnnggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSFVLDSRK SCGFLGSHIH RHHLYDNLET VGSRDQLGTT ENPPDDYYGL NSSDDHYGGA NSVDSVGGTA ELSGSDSAAT SNKDAGSIMI PYSVNYPVHH EELRGVVADE RHEKAKEKLE HLGFFSPLAS ETAHLNTRQR DISGPSDSHS LTMSTLEPES RIWECMKSSS LLQQIGTKQA SVQELTLQSP SRMDYESIRD QWVIGRARIH WFPRYFGKSL GELSEYIKMC DVILDVRDAR IPYVADSDFL LNVYNNMFTH KPKIIVFTHA DLSSINGSDE WATYYRIKNF WEAQAFNRNI PDPNKKPVTP VIFVNARDGI SCIVRLKKLI YKLCHRANQK RLRRGLAARP LRAIALGMPN VGKSALINRL LGRHKARSFN AAGVTRDIRL VRLHPDEYRE AAHKIIDVID TPGVLPANLY NMAFGPVGNK ASRKRNRLLG SIYFKLPRTS VQGGHSKSGI DAIHTGLERN VWLIAAANQI VEGAYDNDQA AENLLEQALH VYRSKRSYVD LPRILKRIGI DYTPSILEMH PEDFSHECLQ VASYKHHGGD LNAAASRILS DFRRGYYGRI TLQLPPDTVS NRYSVPFNGI EEPKPSSTHA NREYSQGLYE GW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtttcgtg ttggacagta ggaaatcgtg cgggttttta ggcagtcaca ttcaccgtca tcatttgtat gacaacctgg aaactgttgg ttcacgtgac cagctgggta ccactgaaaa tcccccggat gattattatg ggctaaatag ctcggatgat cactatggcg gagcaaatag cgttgattcg gttggtggta cagctgaact ttcgggatct gatagcgctg ccacatcaaa caaggatgcg ggtagcatta tgatccctta ctctgtgaac tatccagtcc atcatgagga gcttcgtggt gttgttgctg acgaacgcca tgaaaaggct aaagagaaat tggagcattt aggtttcttt tcacctcttg ccagcgaaac tgcacatttg aacactaggc agcgtgacat atcgggacct tccgacagtc actctttgac gatgtcaact ttggagcctg agagccgcat atgggagtgt atgaagtcat cttcattgtt acagcaaatt ggtactaagc aggcatcggt tcaggaattg acattacaat ctcctagcag gatggattac gagagcatac gtgaccagtg ggttataggc agggctcgca ttcattggtt tccacgttac tttggtaaga gtctgggtga gctctctgag tacatcaaga tgtgtgatgt tattcttgac gttcgcgatg cgaggatccc ttatgttgcg gattccgact ttctattgaa tgtctataac aacatgttta ctcacaagcc taagataatc gtttttactc atgctgattt aagttctatc aatggatctg acgaatgggc cacttattac cgcattaaga acttttggga agcccaggcg ttcaatagga acattcctga tcctaacaag aagccggtga cccctgtgat atttgtcaat gctcgtgacg gaatttcgtg tatagttcgc ttaaagaagt tgatatacaa gttatgccac cgtgcaaatc aaaaacgctt gcgtcgtggt ttagctgcac gcccactgcg tgcaattgct ttgggcatgc ccaatgttgg caagagtgcg cttattaacc gtcttcttgg tcgtcacaaa gctcgtagtt tcaatgcagc gggtgtaact cgtgacatac gactcgtccg tctacaccct gatgagtaca gggaggcagc ccacaagatt atagatgtga tagacactcc tggagtgctg cctgctaatt tgtataacat ggcctttggg ccagttggta acaaggccag tcgtaagcgg aataggttac tgggcagtat atattttaag ctaccgcgca cgagtgttca gggtggccac agcaaaagcg gtattgatgc gatccataca ggtttagagc gcaatgtatg gctcatagct gctgctaacc aaatagttga gggtgcttat gacaacgacc aggcagcgga aaacttgctt gagcaggctt tgcatgtata taggtccaag cgatcgtatg tagatttacc gcgtatattg aagcgtattg gcattgacta tacccctagc atactggaga tgcatccaga ggattttagc cacgagtgcc tgcaggttgc tagttataaa catcacggtg gtgacctcaa tgctgcagcc agccgtatat tgtctgactt taggcgtggt tactatggtc gcataacttt gcaactaccc cctgatactg tgtctaaccg ttattcagta ccattcaacg gtatcgagga acccaaacca agttctactc acgccaatcg tgaatactcc caaggtttat acgagggatg gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa