BaboA.18322.a
apurinic/apyrimidinic endonuclease Apn1
| CENTER ID: | BaboA.18322.a |
| ORGANISM: | Babesia bovis T2Bo |
| ASSOCIATED DISEASE: | Babesiosis (atypical) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaboA.18322.a.A1.GE34066 | full length | 1 | 374 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaboA.18322.a.A1.PS01573 | full length | 1 | 374 | ||
| BaboA.18322.a.A1.PS01574 | full length | 1 | 374 |
Structures
10NH
DEPOSITED: 1/28/2026
DETERMINATION: XRay
CLONE: BaboA.18322.a.B2.GE45625
PROTEIN: BaboA.18322.a.B2.PW39407
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | PiroplasmaDB:BBOV_III005900 |
| RefSeq: | XP_001611720.1 |
| UniProt: | A7ANL8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTTDSFVSD ISDDGSTSSK SSTTKTKKTG ISKGSTKKPL QTKLSVKSST EKPKPWVTLP KLDPNEDRDA AFAEIAAASA ASGLYIGAHI STAGGLDNSV INAYNICGQA FALFLKNQRR WDSPPLADAT VKKFTANIEK YKYDIRYVLP HGSYLINIAN PDYEKRMKSY HHFVDDIQRC EKLGITLYNF HPGSTVGMCE KPEGIRNIAN CINMAMKETS SAKIVLENAA GQKNVIGSTF EDLRDIINLV ENKDRVAVCL DTCHLFAAGY DIRTKDKFEA VMRSFDEIIG LKYLVAVHLN DCKSDLGSGL DRHENIGIGK LTRETFEFIA NSGYFRNMPI ILETPDIHGD ETIYKQEVKV MYGLVEGNKD GSTS
NT Sequence
atgtccacaa cggactcctt tgtgagtgac atttctgacg acggctctac atcttccaaa tcttccacta ccaagaccaa gaagactggc atttcgaagg gttccacgaa gaagccattg cagactaagc ttagtgtcaa gtcatctact gagaagccga agccttgggt aactttacca aagttagacc ctaatgaaga ccgtgacgca gcatttgctg agatagctgc tgcaagtgca gcttctgggc tttatatagg tgctcacata tccactgctg gtggtttgga caactctgtg attaacgctt acaacatttg tggccaggct tttgctttat tcctcaagaa tcagcgtcgt tgggacagtc ctcctcttgc tgacgctact gttaagaagt ttacggccaa cattgagaaa tataaatatg acatccgcta cgtgttgcct catggttctt atttgataaa cattgccaac cctgactacg agaagcgtat gaagtcatat caccacttcg ttgatgatat tcaacgttgt gagaagctgg gcatcacatt gtacaacttt catccaggct ctactgttgg catgtgtgaa aaacctgagg gtattcgcaa catcgcgaac tgcattaaca tggctatgaa ggagactagc tctgcaaaga ttgttttgga gaatgctgct ggtcagaaga acgttatagg ttcaactttt gaggatttac gtgacattat taatttagtt gagaacaagg atagggttgc tgtatgcctg gacacgtgtc atctgtttgc tgctggatac gacattcgaa ctaaggataa gtttgaggca gttatgcgtt cttttgatga gataattggt ttgaaatacc ttgttgctgt acatttgaat gactgcaagt ctgaccttgg cagtggtttg gatcgtcatg agaacattgg tattgggaag ctgactcgtg aaacgtttga gttcattgcc aatagtggat atttccgcaa tatgccaata attttagaga ctcctgatat acacggtgac gaaactatat acaagcagga agttaaggtg atgtacggac ttgttgaagg gaacaaagat ggttccactt cctaa
Details for BaboA.18322.a.A1.GE34066
| HARVESTED ON: | 6/4/2012 |
| SEQUENCED ON: | 6/5/2012 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MSTTDSFVSD ISDDGSTSSK SSTTKTKKTG ISKGSTKKPL QTKLSVKSST EKPKPWVTLP KLDPNEDRDA AFAEIAAASA ASGLYIGAHI STAGGLDNSV INAYNICGQA FALFLKNQRR WDSPPLADAT VKKFTANIEK YKYDIRYVLP HGSYLINIAN PDYEKRMKSY HHFVDDIQRC EKLGITLYNF HPGSTVGMCE KPEGIRNIAN CINMAMKETS SAKIVLENAA GQKNVIGSTF EDLRDIINLV ENKDRVAVCL DTCHLFAAGY DIRTKDKFEA VMRSFDEIIG LKYLVAVHLN DCKSDLGSGL DRHENIGIGK LTRETFEFIA NSGYFRNMPI ILETPDIHGD ETIYKQEVKV MYGLVEGNKD GSTS
Validated NT Sequence
aggaagtgga accatctttg ttcccttcaa caagtccgta catcacctta acttcctgct tgtatatagt ttcgtcaccg tgtatatcag gagtctctaa aattattggc atattgcgga aatatccact attggcaatg aactcaaacg tttcacgagt cagcttccca ataccaatgt tctcatgacg atccaaacca ctgccaaggt cagacttgca gtcattcaaa tgtacagcaa caaggtattt caaaccaatt atctcatcaa aagaacgcat aactgcctca aacttatcct tagttcgaat gtcgtatcca gcagcaaaca gatgacacgt gtccaggcat acagcaaccc tatccttgtt ctcaactaaa ttaataatgt cacgtaaatc ctcaaaagtt gaacctataa cgttcttctg accagcagca ttctccaaaa caatctttgc agagctagtc tccttcatag ccatgttaat gcagttcgcg atgttgcgaa taccctcagg tttttcacac atgccaacag tagagcctgg atgaaagttg tacaatgtga tgcccagctt ctcacaacgt tgaatatcat caacgaagtg gtgatatgac ttcatacgct tctcgtagtc agggttggca atgtttatca aataagaacc atgaggcaac acgtagcgga tgtcatattt atatttctca atgttggccg taaacttctt aacagtagcg tcagcaagag gaggactgtc ccaacgacgc tgattcttga ggaataaagc aaaagcctgg ccacaaatgt tgtaagcgtt aatcacagag ttgtccaaac caccagcagt ggatatgtga gcacctatat aaagcccaga agctgcactt gcagcagcta tctcagcaaa tgctgcgtca cggtcttcat tagggtctaa ctttggtaaa gttacccaag gcttcggctt ctcagtagat gacttgacac taagcttagt ctgcaatggc ttcttcgtgg aacccttcga aatgccagtc ttcttggtct tggtagtgga agatttggaa gatgtagagc cgtcgtcaga aatgtcactc acaaaggagt ccgttgtgga catcgaacca ggaccctggg tctgagcttc caggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSFVS DISDDGSTSS KSSTTKTKKT GISKGSTKKP LQTKLSVKSS TEKPKPWVTL PKLDPNEDRD AAFAEIAAAS AASGLYIGAH ISTAGGLDNS VINAYNICGQ AFALFLKNQR RWDSPPLADA TVKKFTANIE KYKYDIRYVL PHGSYLINIA NPDYEKRMKS YHHFVDDIQR CEKLGITLYN FHPGSTVGMC EKPEGIRNIA NCINMAMKET SSAKIVLENA AGQKNVIGST FEDLRDIINL VENKDRVAVC LDTCHLFAAG YDIRTKDKFE AVMRSFDEII GLKYLVAVHL NDCKSDLGSG LDRHENIGIG KLTRETFEFI ANSGYFRNMP IILETPDIHG DETIYKQEVK VMYGLVEGNK DGSTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccacaacg gactcctttg tgagtgacat ttctgacgac ggctctacat cttccaaatc ttccactacc aagaccaaga agactggcat ttcgaagggt tccacgaaga agccattgca gactaagctt agtgtcaagt catctactga gaagccgaag ccttgggtaa ctttaccaaa gttagaccct aatgaagacc gtgacgcagc atttgctgag atagctgctg caagtgcagc ttctgggctt tatataggtg ctcacatatc cactgctggt ggtttggaca actctgtgat taacgcttac aacatttgtg gccaggcttt tgctttattc ctcaagaatc agcgtcgttg ggacagtcct cctcttgctg acgctactgt taagaagttt acggccaaca ttgagaaata taaatatgac atccgctacg tgttgcctca tggttcttat ttgataaaca ttgccaaccc tgactacgag aagcgtatga agtcatatca ccacttcgtt gatgatattc aacgttgtga gaagctgggc atcacattgt acaactttca tccaggctct actgttggca tgtgtgaaaa acctgagggt attcgcaaca tcgcgaactg cattaacatg gctatgaagg agactagctc tgcaaagatt gttttggaga atgctgctgg tcagaagaac gttataggtt caacttttga ggatttacgt gacattatta atttagttga gaacaaggat agggttgctg tatgcctgga cacgtgtcat ctgtttgctg ctggatacga cattcgaact aaggataagt ttgaggcagt tatgcgttct tttgatgaga taattggttt gaaatacctt gttgctgtac atttgaatga ctgcaagtct gaccttggca gtggtttgga tcgtcatgag aacattggta ttgggaagct gactcgtgaa acgtttgagt tcattgccaa tagtggatat ttccgcaata tgccaataat tttagagact cctgatatac acggtgacga aactatatac aagcaggaag ttaaggtgat gtacggactt gttgaaggga acaaagatgg ttccacttcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BaboA.18322.a.A1.PS01573
| PURIFICATION DATe: | 7/31/2012 |
| CONCENTRATION: | 18.01mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSTTDSFVSD ISDDGSTSSK SSTTKTKKTG ISKGSTKKPL QTKLSVKSST EKPKPWVTLP KLDPNEDRDA AFAEIAAASA ASGLYIGAHI STAGGLDNSV INAYNICGQA FALFLKNQRR WDSPPLADAT VKKFTANIEK YKYDIRYVLP HGSYLINIAN PDYEKRMKSY HHFVDDIQRC EKLGITLYNF HPGSTVGMCE KPEGIRNIAN CINMAMKETS SAKIVLENAA GQKNVIGSTF EDLRDIINLV ENKDRVAVCL DTCHLFAAGY DIRTKDKFEA VMRSFDEIIG LKYLVAVHLN DCKSDLGSGL DRHENIGIGK LTRETFEFIA NSGYFRNMPI ILETPDIHGD ETIYKQEVKV MYGLVEGNKD GSTS
Validated NT Sequence
aggaagtgga accatctttg ttcccttcaa caagtccgta catcacctta acttcctgct tgtatatagt ttcgtcaccg tgtatatcag gagtctctaa aattattggc atattgcgga aatatccact attggcaatg aactcaaacg tttcacgagt cagcttccca ataccaatgt tctcatgacg atccaaacca ctgccaaggt cagacttgca gtcattcaaa tgtacagcaa caaggtattt caaaccaatt atctcatcaa aagaacgcat aactgcctca aacttatcct tagttcgaat gtcgtatcca gcagcaaaca gatgacacgt gtccaggcat acagcaaccc tatccttgtt ctcaactaaa ttaataatgt cacgtaaatc ctcaaaagtt gaacctataa cgttcttctg accagcagca ttctccaaaa caatctttgc agagctagtc tccttcatag ccatgttaat gcagttcgcg atgttgcgaa taccctcagg tttttcacac atgccaacag tagagcctgg atgaaagttg tacaatgtga tgcccagctt ctcacaacgt tgaatatcat caacgaagtg gtgatatgac ttcatacgct tctcgtagtc agggttggca atgtttatca aataagaacc atgaggcaac acgtagcgga tgtcatattt atatttctca atgttggccg taaacttctt aacagtagcg tcagcaagag gaggactgtc ccaacgacgc tgattcttga ggaataaagc aaaagcctgg ccacaaatgt tgtaagcgtt aatcacagag ttgtccaaac caccagcagt ggatatgtga gcacctatat aaagcccaga agctgcactt gcagcagcta tctcagcaaa tgctgcgtca cggtcttcat tagggtctaa ctttggtaaa gttacccaag gcttcggctt ctcagtagat gacttgacac taagcttagt ctgcaatggc ttcttcgtgg aacccttcga aatgccagtc ttcttggtct tggtagtgga agatttggaa gatgtagagc cgtcgtcaga aatgtcactc acaaaggagt ccgttgtgga catcgaacca ggaccctggg tctgagcttc caggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSFVS DISDDGSTSS KSSTTKTKKT GISKGSTKKP LQTKLSVKSS TEKPKPWVTL PKLDPNEDRD AAFAEIAAAS AASGLYIGAH ISTAGGLDNS VINAYNICGQ AFALFLKNQR RWDSPPLADA TVKKFTANIE KYKYDIRYVL PHGSYLINIA NPDYEKRMKS YHHFVDDIQR CEKLGITLYN FHPGSTVGMC EKPEGIRNIA NCINMAMKET SSAKIVLENA AGQKNVIGST FEDLRDIINL VENKDRVAVC LDTCHLFAAG YDIRTKDKFE AVMRSFDEII GLKYLVAVHL NDCKSDLGSG LDRHENIGIG KLTRETFEFI ANSGYFRNMP IILETPDIHG DETIYKQEVK VMYGLVEGNK DGSTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCACAACG GACTCCTTTG TGAGTGACAT TTCTGACGAC GGCTCTACAT CTTCCAAATC TTCCACTACC AAGACCAAGA AGACTGGCAT TTCGAAGGGT TCCACGAAGA AGCCATTGCA GACTAAGCTT AGTGTCAAGT CATCTACTGA GAAGCCGAAG CCTTGGGTAA CTTTACCAAA GTTAGACCCT AATGAAGACC GTGACGCAGC ATTTGCTGAG ATAGCTGCTG CAAGTGCAGC TTCTGGGCTT TATATAGGTG CTCACATATC CACTGCTGGT GGTTTGGACA ACTCTGTGAT TAACGCTTAC AACATTTGTG GCCAGGCTTT TGCTTTATTC CTCAAGAATC AGCGTCGTTG GGACAGTCCT CCTCTTGCTG ACGCTACTGT TAAGAAGTTT ACGGCCAACA TTGAGAAATA TAAATATGAC ATCCGCTACG TGTTGCCTCA TGGTTCTTAT TTGATAAACA TTGCCAACCC TGACTACGAG AAGCGTATGA AGTCATATCA CCACTTCGTT GATGATATTC AACGTTGTGA GAAGCTGGGC ATCACATTGT ACAACTTTCA TCCAGGCTCT ACTGTTGGCA TGTGTGAAAA ACCTGAGGGT ATTCGCAACA TCGCGAACTG CATTAACATG GCTATGAAGG AGACTAGCTC TGCAAAGATT GTTTTGGAGA ATGCTGCTGG TCAGAAGAAC GTTATAGGTT CAACTTTTGA GGATTTACGT GACATTATTA ATTTAGTTGA GAACAAGGAT AGGGTTGCTG TATGCCTGGA CACGTGTCAT CTGTTTGCTG CTGGATACGA CATTCGAACT AAGGATAAGT TTGAGGCAGT TATGCGTTCT TTTGATGAGA TAATTGGTTT GAAATACCTT GTTGCTGTAC ATTTGAATGA CTGCAAGTCT GACCTTGGCA GTGGTTTGGA TCGTCATGAG AACATTGGTA TTGGGAAGCT GACTCGTGAA ACGTTTGAGT TCATTGCCAA TAGTGGATAT TTCCGCAATA TGCCAATAAT TTTAGAGACT CCTGATATAC ACGGTGACGA AACTATATAC AAGCAGGAAG TTAAGGTGAT GTACGGACTT GTTGAAGGGA ACAAAGATGG TTCCACTTCC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for BaboA.18322.a.A1.PS01574
| PURIFICATION DATe: | 7/31/2012 |
| CONCENTRATION: | 5.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSTTDSFVSD ISDDGSTSSK SSTTKTKKTG ISKGSTKKPL QTKLSVKSST EKPKPWVTLP KLDPNEDRDA AFAEIAAASA ASGLYIGAHI STAGGLDNSV INAYNICGQA FALFLKNQRR WDSPPLADAT VKKFTANIEK YKYDIRYVLP HGSYLINIAN PDYEKRMKSY HHFVDDIQRC EKLGITLYNF HPGSTVGMCE KPEGIRNIAN CINMAMKETS SAKIVLENAA GQKNVIGSTF EDLRDIINLV ENKDRVAVCL DTCHLFAAGY DIRTKDKFEA VMRSFDEIIG LKYLVAVHLN DCKSDLGSGL DRHENIGIGK LTRETFEFIA NSGYFRNMPI ILETPDIHGD ETIYKQEVKV MYGLVEGNKD GSTS
Validated NT Sequence
aggaagtgga accatctttg ttcccttcaa caagtccgta catcacctta acttcctgct tgtatatagt ttcgtcaccg tgtatatcag gagtctctaa aattattggc atattgcgga aatatccact attggcaatg aactcaaacg tttcacgagt cagcttccca ataccaatgt tctcatgacg atccaaacca ctgccaaggt cagacttgca gtcattcaaa tgtacagcaa caaggtattt caaaccaatt atctcatcaa aagaacgcat aactgcctca aacttatcct tagttcgaat gtcgtatcca gcagcaaaca gatgacacgt gtccaggcat acagcaaccc tatccttgtt ctcaactaaa ttaataatgt cacgtaaatc ctcaaaagtt gaacctataa cgttcttctg accagcagca ttctccaaaa caatctttgc agagctagtc tccttcatag ccatgttaat gcagttcgcg atgttgcgaa taccctcagg tttttcacac atgccaacag tagagcctgg atgaaagttg tacaatgtga tgcccagctt ctcacaacgt tgaatatcat caacgaagtg gtgatatgac ttcatacgct tctcgtagtc agggttggca atgtttatca aataagaacc atgaggcaac acgtagcgga tgtcatattt atatttctca atgttggccg taaacttctt aacagtagcg tcagcaagag gaggactgtc ccaacgacgc tgattcttga ggaataaagc aaaagcctgg ccacaaatgt tgtaagcgtt aatcacagag ttgtccaaac caccagcagt ggatatgtga gcacctatat aaagcccaga agctgcactt gcagcagcta tctcagcaaa tgctgcgtca cggtcttcat tagggtctaa ctttggtaaa gttacccaag gcttcggctt ctcagtagat gacttgacac taagcttagt ctgcaatggc ttcttcgtgg aacccttcga aatgccagtc ttcttggtct tggtagtgga agatttggaa gatgtagagc cgtcgtcaga aatgtcactc acaaaggagt ccgttgtgga catcgaacca ggaccctggg tctgagcttc caggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSFVS DISDDGSTSS KSSTTKTKKT GISKGSTKKP LQTKLSVKSS TEKPKPWVTL PKLDPNEDRD AAFAEIAAAS AASGLYIGAH ISTAGGLDNS VINAYNICGQ AFALFLKNQR RWDSPPLADA TVKKFTANIE KYKYDIRYVL PHGSYLINIA NPDYEKRMKS YHHFVDDIQR CEKLGITLYN FHPGSTVGMC EKPEGIRNIA NCINMAMKET SSAKIVLENA AGQKNVIGST FEDLRDIINL VENKDRVAVC LDTCHLFAAG YDIRTKDKFE AVMRSFDEII GLKYLVAVHL NDCKSDLGSG LDRHENIGIG KLTRETFEFI ANSGYFRNMP IILETPDIHG DETIYKQEVK VMYGLVEGNK DGSTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCACAACG GACTCCTTTG TGAGTGACAT TTCTGACGAC GGCTCTACAT CTTCCAAATC TTCCACTACC AAGACCAAGA AGACTGGCAT TTCGAAGGGT TCCACGAAGA AGCCATTGCA GACTAAGCTT AGTGTCAAGT CATCTACTGA GAAGCCGAAG CCTTGGGTAA CTTTACCAAA GTTAGACCCT AATGAAGACC GTGACGCAGC ATTTGCTGAG ATAGCTGCTG CAAGTGCAGC TTCTGGGCTT TATATAGGTG CTCACATATC CACTGCTGGT GGTTTGGACA ACTCTGTGAT TAACGCTTAC AACATTTGTG GCCAGGCTTT TGCTTTATTC CTCAAGAATC AGCGTCGTTG GGACAGTCCT CCTCTTGCTG ACGCTACTGT TAAGAAGTTT ACGGCCAACA TTGAGAAATA TAAATATGAC ATCCGCTACG TGTTGCCTCA TGGTTCTTAT TTGATAAACA TTGCCAACCC TGACTACGAG AAGCGTATGA AGTCATATCA CCACTTCGTT GATGATATTC AACGTTGTGA GAAGCTGGGC ATCACATTGT ACAACTTTCA TCCAGGCTCT ACTGTTGGCA TGTGTGAAAA ACCTGAGGGT ATTCGCAACA TCGCGAACTG CATTAACATG GCTATGAAGG AGACTAGCTC TGCAAAGATT GTTTTGGAGA ATGCTGCTGG TCAGAAGAAC GTTATAGGTT CAACTTTTGA GGATTTACGT GACATTATTA ATTTAGTTGA GAACAAGGAT AGGGTTGCTG TATGCCTGGA CACGTGTCAT CTGTTTGCTG CTGGATACGA CATTCGAACT AAGGATAAGT TTGAGGCAGT TATGCGTTCT TTTGATGAGA TAATTGGTTT GAAATACCTT GTTGCTGTAC ATTTGAATGA CTGCAAGTCT GACCTTGGCA GTGGTTTGGA TCGTCATGAG AACATTGGTA TTGGGAAGCT GACTCGTGAA ACGTTTGAGT TCATTGCCAA TAGTGGATAT TTCCGCAATA TGCCAATAAT TTTAGAGACT CCTGATATAC ACGGTGACGA AACTATATAC AAGCAGGAAG TTAAGGTGAT GTACGGACTT GTTGAAGGGA ACAAAGATGG TTCCACTTCC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA