SSGCID: Target Page

BaboA.18230.a

MAK16 protein, putative

CENTER ID:	BaboA.18230.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.18230.a.A1.GE34029	full length		1	226

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III001370
RefSeq:	XP_001611272.1
UniProt:	A7AMC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDDVVWQLV REGFCAFRKC TDTKDFCSNA YNVTGLCNRA SCPLANSNYA TVIEDQGTLF LCMKTAERCH TPSEQWERLK LSKNLETSLN QISEATKVGI AEYLTEKCHL RLKRWREVLQ RMRRQALIQK TKLVPIKKKT ERREKIREKK AEIAARLDNA IEQELLNRLS RGTYGELYQF EQDIDGEIEK EEDEEDVDYA RNKSVKRRAM PHMEIEYEED YERDLA

NT Sequence

atgagtgacg atgtggtatg gcaactcgtc agggaaggtt tctgcgcctt taggaaatgt accgatacaa aagacttctg tagtaatgcc tataatgtca cgggattgtg caatcgagca agctgtccac tggcaaatag caattatgcc actgtaatcg aagatcaagg aacacttttt ctttgtatga agactgcgga aaggtgtcat actccttcgg agcaatggga acggcttaaa ctatcgaaga atttggaaac ttcattgaac caaattagtg aagctactaa agtcggtatc gcagaatatc tcaccgaaaa atgccatttg agactgaaaa gatggcgtga agtgcttcaa agaatgagga gacaagcact catacaaaaa acaaaacttg tccctattaa aaagaaaact gaaaggcgtg aaaaaatacg tgaaaagaag gccgaaatcg cagctagatt ggataatgca atcgagcagg aactacttaa tcgcctttcc aggggtacat acggagaatt ataccagttt gaacaagata ttgatggtga aattgaaaag gaagaagacg aggaagatgt agactacgca aggaataaat ctgtaaagag aagagccatg cctcatatgg aaattgaata tgaagaggat tatgaacgtg atctggcata a

Details for BaboA.18230.a.A1.GE34029

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	28kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSDDVVWQL VREGFCAFRK CTDTKDFCSN AYNVTGLCNR ASCPLANSNY ATVIEDQGTL FLCMKTAERC HTPSEQWERL KLSKNLETSL NQISEATKVG IAEYLTEKCH LRLKRWREVL QRMRRQALIQ KTKLVPIKKK TERREKIREK KAEIAARLDN AIEQELLNRL SRGTYGELYQ FEQDIDGEIE KEEDEEDVDY ARNKSVKRRA MPHMEIEYEE DYERDLA

Validated NT Sequence

ttgttnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgagtgacg atgtggtatg gcaactcgtc agggaaggtt tctgcgcctt taggaaatgt accgatacaa aagacttctg tagtaatgcc tataatgtca cgggattgtg caatcgagca agctgtccac tggcaaatag caattatgcc actgtaatcg aagatcaagg aacacttttt ctttgtatga agactgcgga aaggtgtcat actccttcgg agcaatggga acggcttaaa ctatcgaaga atttggaaac ttcattgaac caaattagtg aagctactaa agtcggtatc gcagaatatc tcaccgaaaa atgccatttg agactgaaaa gatggcgtga agtgcttcaa agaatgagga gacaagcact catacaaaaa acaaaacttg tccctattaa aaagaaaact gaaaggcgtg aaaaaatacg tgaaaagaag gccgaaatcg cagctagatt ggataatgca atcgagcagg aactacttaa tcgcctttcc aggggtacat acggagaatt ataccagttt gaacaagata ttgatggtga aattgaaaag gaagaagacg aggaagatgt agactacgca aggaataaat ctgtaaagag aagagccatg cctcatatgg aaattgaata tgaagaggat tatgaacgtg atctggcata ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg nnnnnnnncc cc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgacgat gtggtatggc aactcgtcag ggaaggtttc tgcgccttta ggaaatgtac cgatacaaaa gacttctgta gtaatgccta taatgtcacg ggattgtgca atcgagcaag ctgtccactg gcaaatagca attatgccac tgtaatcgaa gatcaaggaa cactttttct ttgtatgaag actgcggaaa ggtgtcatac tccttcggag caatgggaac ggcttaaact atcgaagaat ttggaaactt cattgaacca aattagtgaa gctactaaag tcggtatcgc agaatatctc accgaaaaat gccatttgag actgaaaaga tggcgtgaag tgcttcaaag aatgaggaga caagcactca tacaaaaaac aaaacttgtc cctattaaaa agaaaactga aaggcgtgaa aaaatacgtg aaaagaaggc cgaaatcgca gctagattgg ataatgcaat cgagcaggaa ctacttaatc gcctttccag gggtacatac ggagaattat accagtttga acaagatatt gatggtgaaa ttgaaaagga agaagacgag gaagatgtag actacgcaag gaataaatct gtaaagagaa gagccatgcc tcatatggaa attgaatatg aagaggatta tgaacgtgat ctggcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa