SSGCID: Target Page

BaboA.18229.a

vacuolar ATP synthase subunit D, putative

CENTER ID:	BaboA.18229.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.18229.a.A1.GE34032	full length		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III004650
RefSeq:	XP_001611596.1
UniProt:	A7AN94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQILKQKRT NAHLGYSLLK RKSDALASKF RKLLKDTIQG KEKVIEGFNE ASYALSNAVW SAGDFKSLVV ESVGRSAVTL RVRTENVAGV IIPHFELKID PTVDVIANIG LTTGGHVIHS VKTAHLEFLE TLAELASLQV SFMMLEQEIK MTNRRVNALD NLVIPTIDNN LEYIKRELDE LEREEFYRLK MVRNMNQDDD IPKRASTDSQ DVHETGQSEP QTIFESVDED IVV

NT Sequence

atgcttcaaa tactgaagca aaagagaact aacgcgcatc taggatattc gctactaaaa agaaagagtg atgctttagc atcgaagttc agaaagctac ttaaggatac catacagggc aaggaaaagg tgatagaggg atttaacgaa gcgtcgtacg cactctctaa tgccgtgtgg tcagcaggag atttcaaatc actggtagtg gaatctgtag gtagatctgc cgttacactg cgagtacgca cggaaaatgt agccggagtg atcataccac actttgaatt aaaaatcgac cctacagtcg atgttatcgc aaacattgga ttaacaaccg gtggacatgt tatacattcc gtgaaaacag cacatctaga gttcttggaa accctggctg aactggcatc actccaggta tccttcatga tgctggagca ggaaattaaa atgacaaaca ggcgtgtaaa cgcacttgat aatctggtaa taccgacaat cgacaacaac ctggagtaca tcaagcgtga attagatgaa ctagaacgtg aagagttcta tagactgaaa atggtgcgca acatgaacca agatgatgat atcccaaaac gggcgtcaac ggattcacaa gatgtacacg aaactggcca atccgagcca cagacaatat tcgagtcagt agatgaagat atcgttgttt ag

Details for BaboA.18229.a.A1.GE34032

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLQILKQKR TNAHLGYSLL KRKSDALASK FRKLLKDTIQ GKEKVIEGFN EASYALSNAV WSAGDFKSLV VESVGRSAVT LRVRTENVAG VIIPHFELKI DPTVDVIANI GLTTGGHVIH SVKTAHLEFL ETLAELASLQ VSFMMLEQEI KMTNRRVNAL DNLVIPTIDN NLEYIKRELD ELEREEFYRL KMVRNMNQDD DIPKRASTDS QDVHETGQSE PQTIFESVDE DIVV

Validated NT Sequence

ttattaaaca acgatatctt catctactga ctcgaatatt gtctgtggct cggattggcc agtttcgtgt acatcttgtg aatccgttga cgcccgtttt gggatatcat catcttggtt catgttgcgc accattttca gtctatagaa ctcttcacgt tctagttcat ctaattcacg cttgatgtac tccaggttgt tgtcgattgt cggtattacc agattatcaa gtgcgtttac acgcctgttt gtcattttaa tttcctgctc cagcatcatg aaggatacct ggagtgatgc cagttcagcc agggtttcca agaactctag atgtgctgtt ttcacggaat gtataacatg tccaccggtt gttaatccaa tgtttgcgat aacatcgact gtagggtcga tttttaattc aaagtgtggt atgatcactc cggctacatt ttccgtgcgt actcgcagtg taacggcaga tctacctaca gattccacta ccagtgattt gaaatctcct gctgaccaca cggcattaga gagtgcgtac gacgcttcgt taaatccctc tatcaccttt tccttgccct gtatggtatc cttaagtagc tttctgaact tcgatgctaa agcatcactc tttcttttta gtagcgaata tcctagatgc gcgttagttc tcttttgctt cagtatttga agcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttcaaata ctgaagcaaa agagaactaa cgcgcatcta ggatattcgc tactaaaaag aaagagtgat gctttagcat cgaagttcag aaagctactt aaggatacca tacagggcaa ggaaaaggtg atagagggat ttaacgaagc gtcgtacgca ctctctaatg ccgtgtggtc agcaggagat ttcaaatcac tggtagtgga atctgtaggt agatctgccg ttacactgcg agtacgcacg gaaaatgtag ccggagtgat cataccacac tttgaattaa aaatcgaccc tacagtcgat gttatcgcaa acattggatt aacaaccggt ggacatgtta tacattccgt gaaaacagca catctagagt tcttggaaac cctggctgaa ctggcatcac tccaggtatc cttcatgatg ctggagcagg aaattaaaat gacaaacagg cgtgtaaacg cacttgataa tctggtaata ccgacaatcg acaacaacct ggagtacatc aagcgtgaat tagatgaact agaacgtgaa gagttctata gactgaaaat ggtgcgcaac atgaaccaag atgatgatat cccaaaacgg gcgtcaacgg attcacaaga tgtacacgaa actggccaat ccgagccaca gacaatattc gagtcagtag atgaagatat cgttgtttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa