BaboA.17991.a

glutamate dehydrogenase, putative

CENTER ID: BaboA.17991.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.17991.a.A1.GE34038 full length 1 455
BaboA.17991.a.AE1.GE43217 full length 1 455
BaboA.17991.a.AE1.GE43400 full length 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.17991.a.A1.PW34648 full length 1 455

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_IV010390
RefSeq: XP_001610960.1
UniProt: A7AS73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKVTPEEL SALCDALRVR VQQRNNGEYE FIQAFDEVME SLRPIFLEDV RYLDVLEHLS EPERVVMFRV PWTTDSGDKM FNRGFRVQFN SCLGPYKGGL RLHPSVNISI LKFLGFEQIF KNSLTGLNMG GAKGGSDFDP SGKSDNEIRN FCQSFMTELH RHIGPDTDIP AGDIGVGARE IGYLFGQYRR LHNGFDGALT GKGLQWGGSN IRPEATGYGA VYFGCAVLED IFKDTIAGKR CIVSGSGNVA QYTVEKLIEL GAVPITMSDS SGYIIEPEGI TLEGLRYIMA FKNPHSRRIS EYLNYSKTAT FHPGDKPWGE SADIAFPCAT QNEILLRDAE TLVKGGVKLV VEGANMPTHS EAVHYLKENG VILCPGKAAN AGGVLVSGLE MAQNSQRVQW TREKVGTKLK EAIYNIYAQC KAACNRVNAG CDLVAGANIA GFLRVADAFI DQGYI
NT Sequence
atgtcgcgta aagtgacgcc ggaagagctt tcagcgctct gcgacgctct tcgcgttcgt gtgcagcagc gtaataacgg tgaatatgag tttatccaag cgttcgacga ggtaatggaa tctttgcgtc caattttctt ggaagacgtg agatatctcg atgtgttgga acacctgtca gaacccgagc gagttgttat gttccgtgtt ccctggacaa ctgactctgg cgataagatg ttcaaccgtg gatttagggt acaattcaat tcatgtctgg gtccttacaa gggaggactt cgtcttcatc catccgtaaa cattagtatt ttaaagttcc tcggatttga acagatattt aagaacagcc ttacgggttt gaatatgggt ggtgccaagg gaggctctga tttcgatcct tctgggaagt cagacaatga aattaggaat ttctgccagt cctttatgac tgagcttcac aggcacatcg gtcccgatac cgatattcct gctggcgata taggtgttgg tgctcgcgag atcggttacc tctttgggca gtaccgtaga ctccacaacg gttttgacgg agcacttacc ggaaagggtt tgcaatgggg aggctccaat attcgacctg aagccaccgg ctacggcgct gtatactttg gctgcgcagt ccttgaagat atatttaagg acaccattgc tggaaagcgt tgcatcgtga gcggtagtgg caatgtagct cagtacactg tcgaaaagct gatagaactt ggagcggtgc caattactat gagtgactct agtggataca ttatagagcc agagggtata actcttgagg gacttcgata catcatggca ttcaagaacc cacacagccg taggatcagt gagtatttga attactctaa aactgcaact ttccaccctg gtgataaacc atggggagaa tctgcggata tagcattccc ttgtgcaact cagaatgaaa ttttacttcg tgatgccgaa acactggtta agggtggtgt taaactcgtg gttgagggtg ctaacatgcc aacacattca gaagctgtgc actatcttaa ggaaaatggt gtaattttgt gccctgggaa agctgctaat gccggtggtg tcttggttag cggtttggaa atggctcaaa acagtcaacg tgtgcagtgg actcgtgaga aggtgggtac taagcttaag gaggccatat ataacatcta cgcccaatgc aaagcggcgt gtaatagggt caatgccggc tgtgaccttg tagccggtgc caacattgct ggattcctca gggtggctga cgccttcatt gatcagggtt atatttga
Details for BaboA.17991.a.A1.GE34038
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSRKVTPEEL SALCDALRVR VQQRNNGEYE FIQAFDEVME SLRPIFLEDV RYLDVLEHLS EPERVVMFRV PWTTDSGDKM FNRGFRVQFN SCLGPYKGGL RLHPSVNISI LKFLGFEQIF KNSLTGLNMG GAKGGSDFDP SGKSDNEIRN FCQSFMTELH RHIGPDTDIP AGDIGVGARE IGYLFGQYRR LHNGFDGALT GKGLQWGGSN IRPEATGYGA VYFGCAVLED IFKDTIAGKR CIVSGSGNVA QYTVEKLIEL GAVPITMSDS SGYIIEPEGI TLEGLRYIMA FKNPHSRRIS EYLNYSKTAT FHPGDKPWGE SADIAFPCAT QNEILLRDAE TLVKGGVKLV VEGANMPTHS EAVHYLKENG VILCPGKAAN AGGVLVSGLE MAQNSQRVQW TREKVGTKLK EAIYNIYAQC KAACNRVNAG CDLVAGANIA GFLRVADAFI DQG
Validated NT Sequence
aaccctgatc aatgaaggcg tcagccaccc tgaggaatcc agcaatgttg gcaccggcta caaggtcaca gccggcattg accctattac acgccgcttt gcattgggcg tagatgttat atatggcctc cttaagctta gtacccacct tctcacgagt ccactgcaca cgttgactgt tttgagccat ttccaaaccg ctaaccaaga caccaccggc attagcagct ttcccagggc acaaaattac accattttcc ttaagatagt gcacagcttc tgaatgtgtt ggcatgttag caccctcaac cacgagttta acaccaccct taaccagtgt ttcggcatca cgaagtaaaa tttcattctg agttgcacaa gggaatgcta tatccgcaga ttctccccat ggtttatcac cagggtggaa agttgcagtt ttagagtaat tcaaatactc actgatccta cggctgtgtg ggttcttgaa tgccatgatg tatcgaagtc cctcaagagt tataccctct ggctctataa tgtatccact agagtcactc atagtaattg gcaccgctcc aagttctatc agcttttcga cagtgtactg agctacattg ccactaccgc tcacgatgca acgctttcca gcaatggtgt ccttaaatat atcttcaagg actgcgcagc caaagtatac agcgccgtag ccggtggctt caggtcgaat attggagcct ccccattgca aaccctttcc ggtaagtgct ccgtcaaaac cgttgtggag tctacggtac tgcccaaaga ggtaaccgat ctcgcgagca ccaacaccta tatcgccagc aggaatatcg gtatcgggac cgatgtgcct gtgaagctca gtcataaagg actggcagaa attcctaatt tcattgtctg acttcccaga aggatcgaaa tcagagcctc ccttggcacc acccatattc aaacccgtaa ggctgttctt aaatatctgt tcaaatccga ggaactttaa aatactaatg tttacggatg gatgaagacg aagtcctccc ttgtaaggac ccagacatga attgaattgt accctaaatc cacggttgaa catcttatcg ccagagtcag ttgtccaggg aacacggaac ataacaactc gctcgggttc tgacaggtgt tccaacacat cgagatatct cacgtcttcc aagaaaattg gacgcaaaga ttccattacc tcgtcgaacg cttggataaa ctcatattca ccgttattac gctgctgcac acgaacgcga agagcgtcgc agagcgctga aagctcttcc ggcgtcactt tacgcgacat cgaaccagga ccctgggtct gagcttccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKVTPEE LSALCDALRV RVQQRNNGEY EFIQAFDEVM ESLRPIFLED VRYLDVLEHL SEPERVVMFR VPWTTDSGDK MFNRGFRVQF NSCLGPYKGG LRLHPSVNIS ILKFLGFEQI FKNSLTGLNM GGAKGGSDFD PSGKSDNEIR NFCQSFMTEL HRHIGPDTDI PAGDIGVGAR EIGYLFGQYR RLHNGFDGAL TGKGLQWGGS NIRPEATGYG AVYFGCAVLE DIFKDTIAGK RCIVSGSGNV AQYTVEKLIE LGAVPITMSD SSGYIIEPEG ITLEGLRYIM AFKNPHSRRI SEYLNYSKTA TFHPGDKPWG ESADIAFPCA TQNEILLRDA ETLVKGGVKL VVEGANMPTH SEAVHYLKEN GVILCPGKAA NAGGVLVSGL EMAQNSQRVQ WTREKVGTKL KEAIYNIYAQ CKAACNRVNA GCDLVAGANI AGFLRVADAF IDQGYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcgtaaa gtgacgccgg aagagctttc agcgctctgc gacgctcttc gcgttcgtgt gcagcagcgt aataacggtg aatatgagtt tatccaagcg ttcgacgagg taatggaatc tttgcgtcca attttcttgg aagacgtgag atatctcgat gtgttggaac acctgtcaga acccgagcga gttgttatgt tccgtgttcc ctggacaact gactctggcg ataagatgtt caaccgtgga tttagggtac aattcaattc atgtctgggt ccttacaagg gaggacttcg tcttcatcca tccgtaaaca ttagtatttt aaagttcctc ggatttgaac agatatttaa gaacagcctt acgggtttga atatgggtgg tgccaaggga ggctctgatt tcgatccttc tgggaagtca gacaatgaaa ttaggaattt ctgccagtcc tttatgactg agcttcacag gcacatcggt cccgataccg atattcctgc tggcgatata ggtgttggtg ctcgcgagat cggttacctc tttgggcagt accgtagact ccacaacggt tttgacggag cacttaccgg aaagggtttg caatggggag gctccaatat tcgacctgaa gccaccggct acggcgctgt atactttggc tgcgcagtcc ttgaagatat atttaaggac accattgctg gaaagcgttg catcgtgagc ggtagtggca atgtagctca gtacactgtc gaaaagctga tagaacttgg agcggtgcca attactatga gtgactctag tggatacatt atagagccag agggtataac tcttgaggga cttcgataca tcatggcatt caagaaccca cacagccgta ggatcagtga gtatttgaat tactctaaaa ctgcaacttt ccaccctggt gataaaccat ggggagaatc tgcggatata gcattccctt gtgcaactca gaatgaaatt ttacttcgtg atgccgaaac actggttaag ggtggtgtta aactcgtggt tgagggtgct aacatgccaa cacattcaga agctgtgcac tatcttaagg aaaatggtgt aattttgtgc cctgggaaag ctgctaatgc cggtggtgtc ttggttagcg gtttggaaat ggctcaaaac agtcaacgtg tgcagtggac tcgtgagaag gtgggtacta agcttaagga ggccatatat aacatctacg cccaatgcaa agcggcgtgt aatagggtca atgccggctg tgaccttgta gccggtgcca acattgctgg attcctcagg gtggctgacg ccttcattga tcagggttat atttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaboA.17991.a.AE1.GE43217
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 51kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 70
PERCENT COVERAGE: 78
Validated AA Sequence
MCWNTCXNPS ELLCSVFPGQ LTLAIRLVNS MECFTIFQMF NRGFRVQFNS CLGPYKGGLR LHPSVNIXIL KFLGFEQIFK NSLTGLNMGG AKGGSDFXPS GKSDNEIXNF CXSFMTELXR HIGMLYCXRF IYYXVPIPIF LLAIXVLXXA RSVTSLGSTV DSTTVLTEHL PERVCNGEAP IFDXKPPATX LYTLAAQSLX IYLRTPLLXK RCIVSGSGNV AQYTVEKXIX XGAVPITMSD XSGYIIEPEG ITLEGLRYIM AFKNPHSRRI SEYLNYSKTA TFHXGDKPWG ESADIAFPCA TQNEILLRDA ETLVKGGVKL VVEGANMPTH SEAVHYLKEN GVILCPGKAA NAGKWIWRYL YLL
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag caatataacc ctgatcaatg aaggcgtcag ccaccctgag gaatccagca atgttggcac cggctacaag gtcacagccg gcattgaccc tattacacgc cgctttgcat tgggcgtaga tgttatatat ggcctcctta agcttagtac ccaccttctc acgagtccac tgcacacgtt gactgttttg agccatttcc aaaccgctaa ccaagacacc acctataaaa ggtataaata tcgccagatc cacttaccgg cattagcagc tttcccaggg cacaaaatta caccattttc cttaagatag tgcacagctt ctgaatgtgt tggcatgtta gcaccctcaa ccacgagttt aacaccaccc ttaaccagtg tttcggcatc acgaagtaaa atttcattct gagttgcaca agggaatgct atatccgcag attctcccca tggtttatca ccanngtgga aagttgcagt tttagagtaa ttcaaatact cactgatcct acggctgtgt gggttcttga atgccatgat gtatcgaagt ccctcaagag ttataccctc tggctctata atgtatccac tanagtcact catagtaatt ggcaccgctc cnnnntctat cancttttcg acagtgtact gagctacatt gccactaccg ctcacgatgc aacgctttnc cagcaatggt gtccttaaat atatnttcaa ggactgcgca gccaaagtat acagcgncgt agccggtggc ttcnngtcga atattggagc ctccccattg caaacccttt ccggtaagtg ctccgtcaaa accgttgtgg agtctacggt actgcccaaa gaggtaacng atctcgcgan cancaacacn tatatcgcca gcaggaatat cggtatcggg acntaatagt atataaaacg tanacagtat aacataccga tgtgcctgng aagctcagtc ataaaggant ggcagaaatt cntaatttca ttgtctgact tcccagaagg atngaaatca gagcctccct tggcaccacc catattcaaa cccgtaaggc tgttcttaaa tatctgttca aatccgagga actttaaaat antaatgttt acggatggat gaagacgaag tcctcccttg taaggaccca gacatgaatt gaattgtacc ctaaatccac ggttgaacat ctggaatata gtgaagcatt ccattgagtt taccaacctt atcgccagag tcagttgtcc agggaacacg gaacataaca actcgctcgg gttntgacag gtgttccaac acatcgagat atctcacgtc ttccaagaaa attggacgca aagattccat tacctcgcta aacatgtgca atgtatagat aatgttactt actcgaacgc ttggataaan tcatattcac cgttattacg ctgctgcaca cgaacgcgaa gagcgtcgca gagcgctgaa agctcttccg gcgtcacttt acgcgacatc atgggagaga
Expected Protein Sequence
MSRKVTPEEL SALCDALRVR VQQRNNGEYE FIQAFDEVME SLRPIFLEDV RYLDVLEHLS EPERVVMFRV PWTTDSGDKM FNRGFRVQFN SCLGPYKGGL RLHPSVNISI LKFLGFEQIF KNSLTGLNMG GAKGGSDFDP SGKSDNEIRN FCQSFMTELH RHIGPDTDIP AGDIGVGARE IGYLFGQYRR LHNGFDGALT GKGLQWGGSN IRPEATGYGA VYFGCAVLED IFKDTIAGKR CIVSGSGNVA QYTVEKLIEL GAVPITMSDS SGYIIEPEGI TLEGLRYIMA FKNPHSRRIS EYLNYSKTAT FHPGDKPWGE SADIAFPCAT QNEILLRDAE TLVKGGVKLV VEGANMPTHS EAVHYLKENG VILCPGKAAN AGGVLVSGLE MAQNSQRVQW TREKVGTKLK EAIYNIYAQC KAACNRVNAG CDLVAGANIA GFLRVADAFI DQGYIGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcgcgtaaa gtgacgccgg aagagctttc agcgctctgc gacgctcttc gcgttcgtgt gcagcagcgt aataacggtg aatatgagtt tatccaagcg ttcgacgagg taatggaatc tttgcgtcca attttcttgg aagacgtgag atatctcgat gtgttggaac acctgtcaga acccgagcga gttgttatgt tccgtgttcc ctggacaact gactctggcg ataagatgtt caaccgtgga tttagggtac aattcaattc atgtctgggt ccttacaagg gaggacttcg tcttcatcca tccgtaaaca ttagtatttt aaagttcctc ggatttgaac agatatttaa gaacagcctt acgggtttga atatgggtgg tgccaaggga ggctctgatt tcgatccttc tgggaagtca gacaatgaaa ttaggaattt ctgccagtcc tttatgactg agcttcacag gcacatcggt cccgataccg atattcctgc tggcgatata ggtgttggtg ctcgcgagat cggttacctc tttgggcagt accgtagact ccacaacggt tttgacggag cacttaccgg aaagggtttg caatggggag gctccaatat tcgacctgaa gccaccggct acggcgctgt atactttggc tgcgcagtcc ttgaagatat atttaaggac accattgctg gaaagcgttg catcgtgagc ggtagtggca atgtagctca gtacactgtc gaaaagctga tagaacttgg agcggtgcca attactatga gtgactctag tggatacatt atagagccag agggtataac tcttgaggga cttcgataca tcatggcatt caagaaccca cacagccgta ggatcagtga gtatttgaat tactctaaaa ctgcaacttt ccaccctggt gataaaccat ggggagaatc tgcggatata gcattccctt gtgcaactca gaatgaaatt ttacttcgtg atgccgaaac actggttaag ggtggtgtta aactcgtggt tgagggtgct aacatgccaa cacattcaga agctgtgcac tatcttaagg aaaatggtgt aattttgtgc cctgggaaag ctgctaatgc cggtggtgtc ttggttagcg gtttggaaat ggctcaaaac agtcaacgtg tgcagtggac tcgtgagaag gtgggtacta agcttaagga ggccatatat aacatctacg cccaatgcaa agcggcgtgt aatagggtca atgccggctg tgaccttgta gccggtgcca acattgctgg attcctcagg gtggctgacg ccttcattga tcagggttat attgggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for BaboA.17991.a.AE1.GE43400
HARVESTED ON: data unavailable
SEQUENCED ON: 10/1/2019
EXPECTED MW: 51kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 70
PERCENT COVERAGE: 78
Validated AA Sequence
MCWNTCXNPS ELLCSVFPGQ LTLAIRLVNS MECFTIFQMF NRGFRVQFNS CLGPYKGGLR LHPSVNIXIL KFLGFEQIFK NSLTGLNMGG AKGGSDFXPS GKSDNEIXNF CXSFMTELXR HIGMLYCXRF IYYXVPIPIF LLAIXVLXXA RSVTSLGSTV DSTTVLTEHL PERVCNGEAP IFDXKPPATX LYTLAAQSLX IYLRTPLLXK RCIVSGSGNV AQYTVEKXIX XGAVPITMSD XSGYIIEPEG ITLEGLRYIM AFKNPHSRRI SEYLNYSKTA TFHXGDKPWG ESADIAFPCA TQNEILLRDA ETLVKGGVKL VVEGANMPTH SEAVHYLKEN GVILCPGKAA NAGKWIWRYL YLL
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag caatataacc ctgatcaatg aaggcgtcag ccaccctgag gaatccagca atgttggcac cggctacaag gtcacagccg gcattgaccc tattacacgc cgctttgcat tgggcgtaga tgttatatat ggcctcctta agcttagtac ccaccttctc acgagtccac tgcacacgtt gactgttttg agccatttcc aaaccgctaa ccaagacacc acctataaaa ggtataaata tcgccagatc cacttaccgg cattagcagc tttcccaggg cacaaaatta caccattttc cttaagatag tgcacagctt ctgaatgtgt tggcatgtta gcaccctcaa ccacgagttt aacaccaccc ttaaccagtg tttcggcatc acgaagtaaa atttcattct gagttgcaca agggaatgct atatccgcag attctcccca tggtttatca ccanngtgga aagttgcagt tttagagtaa ttcaaatact cactgatcct acggctgtgt gggttcttga atgccatgat gtatcgaagt ccctcaagag ttataccctc tggctctata atgtatccac tanagtcact catagtaatt ggcaccgctc cnnnntctat cancttttcg acagtgtact gagctacatt gccactaccg ctcacgatgc aacgctttnc cagcaatggt gtccttaaat atatnttcaa ggactgcgca gccaaagtat acagcgncgt agccggtggc ttcnngtcga atattggagc ctccccattg caaacccttt ccggtaagtg ctccgtcaaa accgttgtgg agtctacggt actgcccaaa gaggtaacng atctcgcgan cancaacacn tatatcgcca gcaggaatat cggtatcggg acntaatagt atataaaacg tanacagtat aacataccga tgtgcctgng aagctcagtc ataaaggant ggcagaaatt cntaatttca ttgtctgact tcccagaagg atngaaatca gagcctccct tggcaccacc catattcaaa cccgtaaggc tgttcttaaa tatctgttca aatccgagga actttaaaat antaatgttt acggatggat gaagacgaag tcctcccttg taaggaccca gacatgaatt gaattgtacc ctaaatccac ggttgaacat ctggaatata gtgaagcatt ccattgagtt taccaacctt atcgccagag tcagttgtcc agggaacacg gaacataaca actcgctcgg gttntgacag gtgttccaac acatcgagat atctcacgtc ttccaagaaa attggacgca aagattccat tacctcgcta aacatgtgca atgtatagat aatgttactt actcgaacgc ttggataaan tcatattcac cgttattacg ctgctgcaca cgaacgcgaa gagcgtcgca gagcgctgaa agctcttccg gcgtcacttt acgcgacatc atgggagaga
Expected Protein Sequence
MSRKVTPEEL SALCDALRVR VQQRNNGEYE FIQAFDEVME SLRPIFLEDV RYLDVLEHLS EPERVVMFRV PWTTDSGDKM FNRGFRVQFN SCLGPYKGGL RLHPSVNISI LKFLGFEQIF KNSLTGLNMG GAKGGSDFDP SGKSDNEIRN FCQSFMTELH RHIGPDTDIP AGDIGVGARE IGYLFGQYRR LHNGFDGALT GKGLQWGGSN IRPEATGYGA VYFGCAVLED IFKDTIAGKR CIVSGSGNVA QYTVEKLIEL GAVPITMSDS SGYIIEPEGI TLEGLRYIMA FKNPHSRRIS EYLNYSKTAT FHPGDKPWGE SADIAFPCAT QNEILLRDAE TLVKGGVKLV VEGANMPTHS EAVHYLKENG VILCPGKAAN AGGVLVSGLE MAQNSQRVQW TREKVGTKLK EAIYNIYAQC KAACNRVNAG CDLVAGANIA GFLRVADAFI DQGYIGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcgcgtaaa gtgacgccgg aagagctttc agcgctctgc gacgctcttc gcgttcgtgt gcagcagcgt aataacggtg aatatgagtt tatccaagcg ttcgacgagg taatggaatc tttgcgtcca attttcttgg aagacgtgag atatctcgat gtgttggaac acctgtcaga acccgagcga gttgttatgt tccgtgttcc ctggacaact gactctggcg ataagatgtt caaccgtgga tttagggtac aattcaattc atgtctgggt ccttacaagg gaggacttcg tcttcatcca tccgtaaaca ttagtatttt aaagttcctc ggatttgaac agatatttaa gaacagcctt acgggtttga atatgggtgg tgccaaggga ggctctgatt tcgatccttc tgggaagtca gacaatgaaa ttaggaattt ctgccagtcc tttatgactg agcttcacag gcacatcggt cccgataccg atattcctgc tggcgatata ggtgttggtg ctcgcgagat cggttacctc tttgggcagt accgtagact ccacaacggt tttgacggag cacttaccgg aaagggtttg caatggggag gctccaatat tcgacctgaa gccaccggct acggcgctgt atactttggc tgcgcagtcc ttgaagatat atttaaggac accattgctg gaaagcgttg catcgtgagc ggtagtggca atgtagctca gtacactgtc gaaaagctga tagaacttgg agcggtgcca attactatga gtgactctag tggatacatt atagagccag agggtataac tcttgaggga cttcgataca tcatggcatt caagaaccca cacagccgta ggatcagtga gtatttgaat tactctaaaa ctgcaacttt ccaccctggt gataaaccat ggggagaatc tgcggatata gcattccctt gtgcaactca gaatgaaatt ttacttcgtg atgccgaaac actggttaag ggtggtgtta aactcgtggt tgagggtgct aacatgccaa cacattcaga agctgtgcac tatcttaagg aaaatggtgt aattttgtgc cctgggaaag ctgctaatgc cggtggtgtc ttggttagcg gtttggaaat ggctcaaaac agtcaacgtg tgcagtggac tcgtgagaag gtgggtacta agcttaagga ggccatatat aacatctacg cccaatgcaa agcggcgtgt aatagggtca atgccggctg tgaccttgta gccggtgcca acattgctgg attcctcagg gtggctgacg ccttcattga tcagggttat attgggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for BaboA.17991.a.A1.PW34648
PURIFICATION DATe: 7/1/2012
CONCENTRATION: 27mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MSRKVTPEEL SALCDALRVR VQQRNNGEYE FIQAFDEVME SLRPIFLEDV RYLDVLEHLS EPERVVMFRV PWTTDSGDKM FNRGFRVQFN SCLGPYKGGL RLHPSVNISI LKFLGFEQIF KNSLTGLNMG GAKGGSDFDP SGKSDNEIRN FCQSFMTELH RHIGPDTDIP AGDIGVGARE IGYLFGQYRR LHNGFDGALT GKGLQWGGSN IRPEATGYGA VYFGCAVLED IFKDTIAGKR CIVSGSGNVA QYTVEKLIEL GAVPITMSDS SGYIIEPEGI TLEGLRYIMA FKNPHSRRIS EYLNYSKTAT FHPGDKPWGE SADIAFPCAT QNEILLRDAE TLVKGGVKLV VEGANMPTHS EAVHYLKENG VILCPGKAAN AGGVLVSGLE MAQNSQRVQW TREKVGTKLK EAIYNIYAQC KAACNRVNAG CDLVAGANIA GFLRVADAFI DQG
Validated NT Sequence
aaccctgatc aatgaaggcg tcagccaccc tgaggaatcc agcaatgttg gcaccggcta caaggtcaca gccggcattg accctattac acgccgcttt gcattgggcg tagatgttat atatggcctc cttaagctta gtacccacct tctcacgagt ccactgcaca cgttgactgt tttgagccat ttccaaaccg ctaaccaaga caccaccggc attagcagct ttcccagggc acaaaattac accattttcc ttaagatagt gcacagcttc tgaatgtgtt ggcatgttag caccctcaac cacgagttta acaccaccct taaccagtgt ttcggcatca cgaagtaaaa tttcattctg agttgcacaa gggaatgcta tatccgcaga ttctccccat ggtttatcac cagggtggaa agttgcagtt ttagagtaat tcaaatactc actgatccta cggctgtgtg ggttcttgaa tgccatgatg tatcgaagtc cctcaagagt tataccctct ggctctataa tgtatccact agagtcactc atagtaattg gcaccgctcc aagttctatc agcttttcga cagtgtactg agctacattg ccactaccgc tcacgatgca acgctttcca gcaatggtgt ccttaaatat atcttcaagg actgcgcagc caaagtatac agcgccgtag ccggtggctt caggtcgaat attggagcct ccccattgca aaccctttcc ggtaagtgct ccgtcaaaac cgttgtggag tctacggtac tgcccaaaga ggtaaccgat ctcgcgagca ccaacaccta tatcgccagc aggaatatcg gtatcgggac cgatgtgcct gtgaagctca gtcataaagg actggcagaa attcctaatt tcattgtctg acttcccaga aggatcgaaa tcagagcctc ccttggcacc acccatattc aaacccgtaa ggctgttctt aaatatctgt tcaaatccga ggaactttaa aatactaatg tttacggatg gatgaagacg aagtcctccc ttgtaaggac ccagacatga attgaattgt accctaaatc cacggttgaa catcttatcg ccagagtcag ttgtccaggg aacacggaac ataacaactc gctcgggttc tgacaggtgt tccaacacat cgagatatct cacgtcttcc aagaaaattg gacgcaaaga ttccattacc tcgtcgaacg cttggataaa ctcatattca ccgttattac gctgctgcac acgaacgcga agagcgtcgc agagcgctga aagctcttcc ggcgtcactt tacgcgacat cgaaccagga ccctgggtct gagcttccag g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKVTPEE LSALCDALRV RVQQRNNGEY EFIQAFDEVM ESLRPIFLED VRYLDVLEHL SEPERVVMFR VPWTTDSGDK MFNRGFRVQF NSCLGPYKGG LRLHPSVNIS ILKFLGFEQI FKNSLTGLNM GGAKGGSDFD PSGKSDNEIR NFCQSFMTEL HRHIGPDTDI PAGDIGVGAR EIGYLFGQYR RLHNGFDGAL TGKGLQWGGS NIRPEATGYG AVYFGCAVLE DIFKDTIAGK RCIVSGSGNV AQYTVEKLIE LGAVPITMSD SSGYIIEPEG ITLEGLRYIM AFKNPHSRRI SEYLNYSKTA TFHPGDKPWG ESADIAFPCA TQNEILLRDA ETLVKGGVKL VVEGANMPTH SEAVHYLKEN GVILCPGKAA NAGGVLVSGL EMAQNSQRVQ WTREKVGTKL KEAIYNIYAQ CKAACNRVNA GCDLVAGANI AGFLRVADAF IDQGYI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCGTAAA GTGACGCCGG AAGAGCTTTC AGCGCTCTGC GACGCTCTTC GCGTTCGTGT GCAGCAGCGT AATAACGGTG AATATGAGTT TATCCAAGCG TTCGACGAGG TAATGGAATC TTTGCGTCCA ATTTTCTTGG AAGACGTGAG ATATCTCGAT GTGTTGGAAC ACCTGTCAGA ACCCGAGCGA GTTGTTATGT TCCGTGTTCC CTGGACAACT GACTCTGGCG ATAAGATGTT CAACCGTGGA TTTAGGGTAC AATTCAATTC ATGTCTGGGT CCTTACAAGG GAGGACTTCG TCTTCATCCA TCCGTAAACA TTAGTATTTT AAAGTTCCTC GGATTTGAAC AGATATTTAA GAACAGCCTT ACGGGTTTGA ATATGGGTGG TGCCAAGGGA GGCTCTGATT TCGATCCTTC TGGGAAGTCA GACAATGAAA TTAGGAATTT CTGCCAGTCC TTTATGACTG AGCTTCACAG GCACATCGGT CCCGATACCG ATATTCCTGC TGGCGATATA GGTGTTGGTG CTCGCGAGAT CGGTTACCTC TTTGGGCAGT ACCGTAGACT CCACAACGGT TTTGACGGAG CACTTACCGG AAAGGGTTTG CAATGGGGAG GCTCCAATAT TCGACCTGAA GCCACCGGCT ACGGCGCTGT ATACTTTGGC TGCGCAGTCC TTGAAGATAT ATTTAAGGAC ACCATTGCTG GAAAGCGTTG CATCGTGAGC GGTAGTGGCA ATGTAGCTCA GTACACTGTC GAAAAGCTGA TAGAACTTGG AGCGGTGCCA ATTACTATGA GTGACTCTAG TGGATACATT ATAGAGCCAG AGGGTATAAC TCTTGAGGGA CTTCGATACA TCATGGCATT CAAGAACCCA CACAGCCGTA GGATCAGTGA GTATTTGAAT TACTCTAAAA CTGCAACTTT CCACCCTGGT GATAAACCAT GGGGAGAATC TGCGGATATA GCATTCCCTT GTGCAACTCA GAATGAAATT TTACTTCGTG ATGCCGAAAC ACTGGTTAAG GGTGGTGTTA AACTCGTGGT TGAGGGTGCT AACATGCCAA CACATTCAGA AGCTGTGCAC TATCTTAAGG AAAATGGTGT AATTTTGTGC CCTGGGAAAG CTGCTAATGC CGGTGGTGTC TTGGTTAGCG GTTTGGAAAT GGCTCAAAAC AGTCAACGTG TGCAGTGGAC TCGTGAGAAG GTGGGTACTA AGCTTAAGGA GGCCATATAT AACATCTACG CCCAATGCAA AGCGGCGTGT AATAGGGTCA ATGCCGGCTG TGACCTTGTA GCCGGTGCCA ACATTGCTGG ATTCCTCAGG GTGGCTGACG CCTTCATTGA TCAGGGTTAT ATTTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA