SSGCID: Target Page

BaboA.17369.a

ATP synthase beta chain, putative

CENTER ID:	BaboA.17369.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.17369.a.A1.GE34098	full length		1	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_IV005830
RefSeq:	XP_001610512.1
UniProt:	A7AQX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCALFRSISG SIGRCLHRSV LHPTRRFSGN ATTSSHLQKG SISQVIGAIV DVKFDGTPPP ILNALWVDTP EGRLSLEVAQ HLGNGVARTI AMSATEGLTR GQEVVDSGNP ITIPVGESTL GRIMNVTGDP LDGCGPIPDS KRLSIHREAV SFSDQRTDEA LLITGIKVVD LLAPYAKGGK IGLFGGAGVG KTVLIMELIN NVAKKHGGFS VFAGVGERTR EGNELYHEMM ETGVIKRRQL EDGTFDFSGS KAALVYGQMN EPPGARARVA LTGLTVAEYF RDEEGQDVLL FIDNIYRFTQ AGSEVSALLG RIPSAVGYQP TLATDLGALQ ERITTTNKGS ITSVQAVYVP ADDITDPAPA TTFSHLDATT VLSRSIAELG IYPAVDPLDS TSRMLSANIV GQEQYDAARG VQKILQDYKS LQDIIAILGM DELSEQDKFV VARARKVQRF LSQPFQVAEV FTGKPGRFVE LQDTISGVKE ILEGECDDMP EMAFYMVGGL QEAKEKAAEM SKTK

NT Sequence

atgtgtgcct tatttcgtag tatcagcggt agtataggcc gttgcctaca ccgctcggta ttgcatccca cccgccgttt ttccggcaac gccacgacct ccagccacct acagaaaggg agcatatccc aggttatcgg cgctatcgtg gatgtcaaat tcgatggaac gccaccgcct atattgaatg cattatgggt tgacacaccg gaaggacgtc ttagcctgga agtagctcaa cacctcggta acggtgttgc acgcacgatc gcaatgagtg caaccgaagg tcttacgcgc ggtcaggaag ttgtagacag tggtaaccca atcacaatcc cagtagggga atccaccctg ggtcgtatca tgaacgttac cggtgaccct cttgatggtt gcggtcccat cccggattcc aagcggttgt cgatccaccg tgaagccgtg tcgttcagtg accaacgtac agatgaagcc ttgctgatca cgggtatcaa agtggttgat ttgttggcac cctatgccaa gggaggtaaa attggtttgt tcggaggtgc cggtgtaggt aaaacggtcc tgattatgga attgatcaac aatgtcgcca agaaacacgg tgggttctcc gtgttcgctg gtgttggtga acgtacgagg gaaggtaacg aactgtacca tgaaatgatg gaaacgggtg tcatcaagcg ccgtcaactg gaagacggaa catttgactt ctcgggctct aaagctgctt tggtgtacgg acaaatgaac gaaccaccag gtgctagagc ccgtgttgca ctcacgggat tgaccgttgc cgagtatttc cgtgatgaag aggggcagga tgtgctactc ttcatcgata acatctaccg tttcacccag gccggttccg aagtgagtgc gctgttagga agaattccat ccgccgtggg ttatcaacct acattggcca ctgatctcgg agcactccag gaacgcatta ctacaaccaa caagggttcg attacatcag tccaggcagt atacgtccca gccgatgata tcactgatcc cgctccagct accactttct cgcacttgga tgccactaca gtgctttctc gttcaattgc ggagttgggt atttaccctg cggtcgaccc gcttgactca acgtcacgta tgctgtcggc caacattgta ggacaggaac agtacgatgc cgcacgtggt gtacagaaaa ttttacagga ctacaaatca ctgcaggata tcattgccat tctgggtatg gacgagctgt ctgagcagga caagttcgtt gtagcacgcg cccgtaaggt acagcgtttc ctgtctcagc cgttccaagt ggctgaggtg ttcaccggca agcctgggag gttcgttgaa ctacaggata ccatcagcgg tgtcaaggaa atcttggaag gtgagtgtga cgatatgccc gaaatggcgt tctacatggt tggtggtctg caagaagcaa aggaaaaggc tgcagaaatg tcaaagacca agtga

Details for BaboA.17369.a.A1.GE34098

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	57kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

cnnnnnnnan ntttgtttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtgtg ccttatttcg tagtatcagc ggtagtatag gccgttgcct acaccgctcg gtattgcatc ccacccgccg tttttccggc aacgccacga cctccagcca cctacagaaa gggagcatat cccaggttat cggcgctatc gtggatgtca aattcgatgg aacgccaccg cctatattga atgcattatg ggttgacaca ccggaaggac gtcttagcct ggaagtagct caacacctcg gtaacggtgt tgcacgcacg atcgcaatga gtgcaaccga aggtcttacg cgcggtcagg aagttgtaga cagtggtaac ccaatcacaa tcccagtagg ggaatccacc ctgggtcgta tcatgaacgt taccggtgac cctcttgatg gttgcggtcc catcccggat tccaagcggt tgtcgatcca ccgtgaagcc gtgtcgttca gtgaccaacg tacagatgaa gccttgctga tcacgggtat caaagtggtt gatttgttgg caccctatgc caagggaggt aaaattggtt tgttcggagg tgccggtgta ggtaaaacgg tcctgattat ggaattgatc aacaatgtcg ccaagaaaca cggtgggttc tccgtgttcg ctggtgttgg tgaacgtacg agggaaggta acgaactgta ccatgaaatg atggaaacgg gtgtcatcaa gcgccgtcaa ctggaanacg gaacatttga cttctcgggc tctaaagctg ctttggtgta cggacaaatg aacgaaccac cnngngctag agcccgtgnt gcactcacgg gantgacngt tgccgagtat ttncgtgnng aanaggggca ggatgtgcta ctcttcatcg ananntctan ngtttcancn nnnnnntccn aantgagtgn nctgtagana ntncatccnc nnggntntna nnannttngn nnctgatnnn gnnnnnnnga nncntnctnn nnnnnnnnnn nnnnnnnagn ncagnnnnnn cntncnncna ngnannnnat ccnnntcnnn cnnccnnttn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMCALFRSIS GSIGRCLHRS VLHPTRRFSG NATTSSHLQK GSISQVIGAI VDVKFDGTPP PILNALWVDT PEGRLSLEVA QHLGNGVART IAMSATEGLT RGQEVVDSGN PITIPVGEST LGRIMNVTGD PLDGCGPIPD SKRLSIHREA VSFSDQRTDE ALLITGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKKHGGF SVFAGVGERT REGNELYHEM METGVIKRRQ LEDGTFDFSG SKAALVYGQM NEPPGARARV ALTGLTVAEY FRDEEGQDVL LFIDNIYRFT QAGSEVSALL GRIPSAVGYQ PTLATDLGAL QERITTTNKG SITSVQAVYV PADDITDPAP ATTFSHLDAT TVLSRSIAEL GIYPAVDPLD STSRMLSANI VGQEQYDAAR GVQKILQDYK SLQDIIAILG MDELSEQDKF VVARARKVQR FLSQPFQVAE VFTGKPGRFV ELQDTISGVK EILEGECDDM PEMAFYMVGG LQEAKEKAAE MSKTK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgtgcctta tttcgtagta tcagcggtag tataggccgt tgcctacacc gctcggtatt gcatcccacc cgccgttttt ccggcaacgc cacgacctcc agccacctac agaaagggag catatcccag gttatcggcg ctatcgtgga tgtcaaattc gatggaacgc caccgcctat attgaatgca ttatgggttg acacaccgga aggacgtctt agcctggaag tagctcaaca cctcggtaac ggtgttgcac gcacgatcgc aatgagtgca accgaaggtc ttacgcgcgg tcaggaagtt gtagacagtg gtaacccaat cacaatccca gtaggggaat ccaccctggg tcgtatcatg aacgttaccg gtgaccctct tgatggttgc ggtcccatcc cggattccaa gcggttgtcg atccaccgtg aagccgtgtc gttcagtgac caacgtacag atgaagcctt gctgatcacg ggtatcaaag tggttgattt gttggcaccc tatgccaagg gaggtaaaat tggtttgttc ggaggtgccg gtgtaggtaa aacggtcctg attatggaat tgatcaacaa tgtcgccaag aaacacggtg ggttctccgt gttcgctggt gttggtgaac gtacgaggga aggtaacgaa ctgtaccatg aaatgatgga aacgggtgtc atcaagcgcc gtcaactgga agacggaaca tttgacttct cgggctctaa agctgctttg gtgtacggac aaatgaacga accaccaggt gctagagccc gtgttgcact cacgggattg accgttgccg agtatttccg tgatgaagag gggcaggatg tgctactctt catcgataac atctaccgtt tcacccaggc cggttccgaa gtgagtgcgc tgttaggaag aattccatcc gccgtgggtt atcaacctac attggccact gatctcggag cactccagga acgcattact acaaccaaca agggttcgat tacatcagtc caggcagtat acgtcccagc cgatgatatc actgatcccg ctccagctac cactttctcg cacttggatg ccactacagt gctttctcgt tcaattgcgg agttgggtat ttaccctgcg gtcgacccgc ttgactcaac gtcacgtatg ctgtcggcca acattgtagg acaggaacag tacgatgccg cacgtggtgt acagaaaatt ttacaggact acaaatcact gcaggatatc attgccattc tgggtatgga cgagctgtct gagcaggaca agttcgttgt agcacgcgcc cgtaaggtac agcgtttcct gtctcagccg ttccaagtgg ctgaggtgtt caccggcaag cctgggaggt tcgttgaact acaggatacc atcagcggtg tcaaggaaat cttggaaggt gagtgtgacg atatgcccga aatggcgttc tacatggttg gtggtctgca agaagcaaag gaaaaggctg cagaaatgtc aaagaccaag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa