BaboA.17242.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD/YgbP)

CENTER ID:	BaboA.17242.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.17242.a.D13.PD00206	Full length( BaboA.17242.a )		60	421

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III003490
OrthoMCL:	OG5_129829
RefSeq:	XP_001611481.1
UniProt:	A7AMX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEYILLHFTE DKEGCLMPVS ALYFLAVYES TFGTPPFHGT KAIPRGNPLF AHNNDLGRCQ KGASAILVCG GVGQRINDVF DNLDSSNANV TILDNCPRAK QFILVHGVPI FIYSFAEFVR SSLVSEVTIA TKPEWHLKVL ELVDCYTQKV PSNEAFNPED ISNRIENPLH KDYIIHVNGR SDSGYMIPRE ASIYPADSAR IEEYRLHLSR YSFVLFDLDS QRCVLSTSSL WPEALSIACS GSPGRYKLVK FCLSGDTRGS TVYNSLTGQN VLGAEFPVVT SSDGIVLVHD AARPMLRRVD MGHLIASARR YGGAIPAIGV VDTIKVGEST GDVNFVFDTP VRSSLFAVQT PQAFVFSVLR TAYEKAMESG TMSMLTDDSS FVERLGTHRV CFVPGHRMNI KLTTSYDLPI CSSYLKEIYF P

NT Sequence

ATGGAATATA TCCTTCTACA TTTCACGGAA GACAAGGAAG GTTGCCTTAT GCCGGTATCG GCGTTATATT TCTTGGCGGT ATATGAATCC ACATTTGGTA CACCTCCGTT CCATGGGACT AAAGCAATTC CTCGTGGCAA TCCATTGTTT GCCCACAATA ACGATCTAGG AAGGTGCCAA AAGGGTGCTT CTGCGATATT GGTTTGCGGT GGTGTTGGCC AACGTATCAA TGATGTCTTT GACAATTTGG ACTCCAGTAA TGCTAACGTT ACTATATTAG ACAACTGCCC ACGGGCTAAA CAGTTCATCC TGGTTCACGG AGTTCCCATT TTTATCTATT CATTTGCTGA GTTTGTAAGA TCATCGTTAG TCAGTGAGGT CACTATAGCT ACAAAGCCTG AATGGCATTT GAAGGTGCTG GAGCTCGTTG ATTGTTACAC TCAGAAGGTT CCTTCTAATG AAGCCTTTAA TCCCGAGGAC ATATCAAATC GCATTGAGAA TCCGTTGCAC AAGGACTACA TAATTCACGT TAATGGCCGT AGCGATTCTG GTTACATGAT TCCTAGGGAG GCTAGTATTT ACCCTGCTGA CAGTGCTAGG ATTGAGGAGT ATCGTTTACA TTTATCGCGG TATTCATTTG TACTATTCGA TCTGGATTCT CAACGTTGTG TTTTGTCTAC ATCATCGCTA TGGCCCGAGG CACTATCTAT AGCTTGCAGT GGTTCCCCGG GTAGATATAA GCTTGTAAAG TTCTGTTTAA GTGGTGACAC TCGTGGTTCA ACTGTGTACA ACTCTCTTAC TGGTCAGAAC GTATTGGGTG CAGAATTCCC AGTGGTTACT AGTTCTGACG GCATCGTATT GGTCCACGAT GCGGCGAGAC CAATGTTACG TCGTGTTGAT ATGGGCCACC TCATAGCGTC TGCTCGTAGG TATGGTGGTG CCATACCGGC TATAGGTGTT GTTGATACTA TAAAAGTTGG TGAAAGCACG GGTGATGTTA ACTTTGTATT TGATACACCG GTTCGGTCAT CACTATTCGC TGTGCAGACT CCGCAGGCTT TTGTTTTTTC TGTGCTCAGG ACAGCCTATG AGAAGGCTAT GGAGTCTGGT ACTATGTCTA TGTTGACTGA CGATTCCAGT TTCGTTGAGC GGCTTGGCAC GCATCGCGTA TGTTTCGTTC CTGGCCATCG CATGAACATT AAGTTGACTA CTAGTTATGA TTTGCCTATA TGCAGCAGTT ATTTGAAGGA GATATATTTT CCG

Details for BaboA.17242.a.D13.PD00206

PURIFICATION DATe:	4/29/2011
CONCENTRATION:	20.35mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGQKGA SAILVCGGVG QRINDVFDNL DSSNANVTIL DNCPRAKQFI LVHGVPIFIY SFAEFVRSSL VSEVTIATKP EWHLKVLELV DCYTQKVPSN EAFNPEDISN RIENPLHKDY IIHVNGRSDS GYMIPREASI YPADSARIEE YRLHLSRYSF VLFDLDSQRC VLSTSSLWPE ALSIACSGSP GRYKLVKFCL SGDTRGSTVY NSLTGQNVLG AEFPVVTSSD GIVLVHDAAR PMLRRVDMGH LIASARRYGG AIPAIGVVDT IKVGESTGDV NFVFDTPVRS SLFAVQTPQA FVFSVLRTAY EKAMESGTMS MLTDDSSFVE RLGTHRVCFV PGHRMNIKLT TSYDLPICSS YLKEIYFP

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGACA GAAAGGTGCG TCTGCCATCC TGGTTTGCGG TGGTGTTGGT CAGCGTATCA ACGATGTGTT CGATAACCTT GACAGCTCTA ACGCTAACGT GACCATCCTG GATAACTGTC CACGTGCTAA ACAGTTTATC CTGGTTCACG GCGTCCCGAT CTTCATCTAC AGCTTCGCTG AATTCGTACG CTCTAGCTTA GTTTCCGAAG TTACCATTGC CACCAAACCT GAATGGCACC TCAAAGTTCT GGAATTAGTT GACTGCTACA CCCAGAAAGT TCCTTCTAAC GAAGCATTCA ACCCAGAAGA CATCTCCAAT CGTATCGAGA ACCCGCTGCA CAAAGACTAC ATAATCCACG TGAACGGCCG TTCTGATAGC GGCTACATGA TCCCACGCGA AGCATCTATC TACCCTGCAG ACTCGGCTCG TATTGAAGAG TACCGTCTGC ACCTGAGCCG TTACAGCTTC GTTCTGTTTG ACCTTGACTC CCAACGCTGC GTTCTCTCTA CGTCCTCCCT CTGGCCTGAA GCACTCTCTA TAGCTTGCTC GGGTAGCCCA GGTCGCTATA AATTAGTTAA ATTCTGTCTG TCCGGCGATA CCCGCGGTAG CACTGTGTAT AACTCTCTGA CCGGCCAGAA TGTGCTGGGT GCCGAATTCC CTGTTGTCAC TAGCAGCGAC GGTATCGTGC TGGTGCATGA CGCTGCACGC CCTATGCTCC GTCGTGTTGA CATGGGTCAT CTGATCGCTA GCGCTCGTCG CTACGGCGGC GCAATCCCGG CTATCGGTGT TGTAGACACT ATCAAAGTAG GCGAATCTAC CGGTGACGTT AATTTCGTGT TCGATACTCC GGTTCGTAGC TCCCTGTTTG CCGTTCAAAC TCCTCAGGCC TTCGTATTCT CCGTGCTGCG CACCGCATAT GAGAAAGCTA TGGAATCCGG CACCATGAGC ATGCTGACTG ACGATAGCTC ATTTGTGGAA CGTTTAGGCA CCCACCGCGT TTGTTTCGTA CCGGGCCACC GCATGAATAT CAAACTCACC ACCTCCTATG ACCTCCCGAT TTGCTCCTCC TATCTGAAAG AAATCTACTT CCCGTAAAAG CTTGCGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGAT